• 제목/요약/키워드: Cytochrome c oxidase subunit 1

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DNA Barcoding for the Hydrothermal Vent Crab Austinograea Species (Crustacea: Bythograeidae) from the North Fiji Basin, Southwestern Pacific Ocean

  • Lee, Won-Kyung;Ju, Se-Jong;Hou, Bo Kyeng;Kim, Se-Joo
    • Animal Systematics, Evolution and Diversity
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    • 제35권1호
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    • pp.30-32
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    • 2019
  • The brachyuran crab Bythograeidae Williams, 1980 is common in hydrothermal vent fields worldwide and has recorded to sixteen species of six genera. In this study, we firstly determined the cytochrome c oxidase subunit 1 (CO1) DNA barcodes for the fifth species of Austinograea, A. hourdezi, from hydrothermal vent regions of the North Fiji Basin in southwestern Pacific Ocean. All CO1 DNA barcodes of A. hourdezi were identical. The interspecies variations of three bythograeid genera were 10.9-13.3% for Austinograea, 6.6-15.7% for Bythograea, and 9.7% for Gandalfus. These results would be helpful to understand taxonomy of brachyuran crabs living in hydrothermal vent fields using CO1 DNA barcodes.

First Genetic Data of Nebalia koreana (Malacostraca, Leptostraca) with DNA Barcode Divergence among Nebalia Species

  • Song, Ji-Hun;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • 제35권1호
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    • pp.37-39
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    • 2019
  • We determined the cytochrome c oxidase subunit 1 (CO1) sequences of Nebalia koreana Song, Moreira & Min, 2012 (Leptostraca) collected from five locations in South Korea, and this represents the first genetic data of this species. The maximum intra-species variation was 1.2% within Nebalia hessleri Martin, Vetter & Cash-Clark, 1996, while inter-species variation ranged from 9.0% (N. hessleri and Nebalia gerkenae Haney & Martin, 2000) to 34.8% (N. hessleri and Nebalia pseudotroncosoi Song, Moreira & Min, 2013). This result is well agreed with the interspecific relationships among Nebalia species based on morphological characteristics. In conclusion, this study showed the usefulness of CO1 sequences as a DNA barcode within the genus Nebalia Leach, 1814.

One New Species and One Unrecorded Species of the Genus Coleophora (Lepidoptera, Coleophoridae) from Korea

  • Koo, Jun-Mo;Baldizzone, Giorgio;Kim, Jae-Dong;Park, Kyu-Tek;Cho, Soowon
    • Animal Systematics, Evolution and Diversity
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    • 제36권3호
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    • pp.193-201
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    • 2020
  • Family Coleophoridae, commonly known as "casebearers", is one of the largest families of Gelechioidea (Lepidoptera), with more than 1,450 described species worldwide, but it has been poorly known in Korea, with only 32 known species of the genus Coleophora Hübner, 1822. Here we present Coleophora fasciella Koo & Baldizzone, sp. nov., a new species to science, and C. mayrella (Hübner, [1813]), an unrecorded species in Korea. Diagnostic characteristics with descriptions of the genitalia are provided with photos of adults, wing venations, and genitalia of both sexes for the species. Mitochondrial cytochrome c oxidase subunit I(COI) barcode sequences for the two species are also provided.

Infection of Taenia asiatica in a Bai Person in Dali, China

  • Wang, Li;Luo, Xuenong;Hou, Junling;Guo, Aijiang;Zhang, Shaohua;Li, Hailong;Cai, Xuepeng
    • Parasites, Hosts and Diseases
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    • 제54권1호
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    • pp.67-70
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    • 2016
  • We report here a human case of Taenia asiatica infection which was confirmed by genetic analyses in Dali, China. A patient was found to have symptoms of taeniasis with discharge of tapeworm proglottids. By sequencing of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene, we observed nucleotide sequence identity of 99% with T. asiatica and 96% with T. saginata. Using the cytochrome b (cytb) gene, 99% identity with T. asiatica and 96% identity with T. saginata were found. Our findings suggest that taeniasis of people in Dali, China may be mainly caused by T. asiatica.

Molecular Identification of Diphyllobothrium nihonkaiense from 3 Human Cases in Heilongjiang Province with a Brief Literature Review in China

  • Zhang, Weizhe;Che, Fei;Tian, Song;Shu, Jing;Zhang, Xiaoli
    • Parasites, Hosts and Diseases
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    • 제53권6호
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    • pp.683-688
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    • 2015
  • Human diphyllobothriasis is a widespread fish-borne zoonosis caused by the infection with broad tapeworms belonging to the genus Diphyllobothrium. In mainland China, so far 20 human cases of Diphyllobothrium infections have been reported, and the etiologic species were identified as D. latum and D. nihonkaiense based on morphological characteristics or molecular analysis. In the present study, proglottids of diphyllobothriid tapeworms from 3 human cases that occurred in Heilongjiang Province, China were identified as D. nihonkaiense by sequencing mitochondrial cytochrome c oxidase subunit I (cox1) and NADH dehydrogenase subunit 5 (nad5) genes. Two different cox1 gene sequences were obtained. One sequence showed 100% homology with those from humans in Japan. The remaining cox1 gene sequence and 2 different nad5 gene sequences obtained were not described previously, and might reflect endemic genetic characterizations. D. nihonkaiense might also be a major causative species of human diphyllobothriasis in China. Meanwhile, the finding of the first pediatric case of D. nihonkaiense infection in China suggests that infants infected with D. nihonkaiense should not be ignored.

First Record of Platorchestia monodi (Crustacea: Amphipoda: Talitridae) from the Pacific Region

  • Kim, Min-Seop;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • 제27권3호
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    • pp.205-212
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    • 2011
  • Platorchestia monodi (Mateus et al., 1986), the beach hopper, commonly lives in sea shores and was previously reported only from the Atlantic regions, such as Mid-Atlantic islands, Brazilian coast, and Israel. We present the first record of this species in the Pacific region. The present species can be clearly distinguished from related species by the shapes of gnathopods 1-2 and development of sexual dimorphism in antenna 2 and pereopod 7. Descriptions of the diagnostic characteristics of the species are provided on the text. In addition, this paper also provided the partial sequence of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene of the species for its molecular characteristics.

Reassessment of the Taxonomic Status of Four Pagurus Species (Crustacea: Decapoda: Paguridae) in Korea Using DNA Barcoding

  • Jung, Jibom;Kim, Won
    • Animal Systematics, Evolution and Diversity
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    • 제36권1호
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    • pp.10-14
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    • 2020
  • Pagurus is the most diverse hermit crab genus in Korea. In this study, the cytochrome c oxidase subunit I (COI) and 16S rRNA of 24 individuals from four Korean Pagurus species (i.e., 7 Pagurus brachiomastus, 8 P. proximus, 8 P. simulans, and 1 P. rectidactylus) were sequenced and analyzed. No genetic difference was found between the COI and 16S rRNA sequences of P. brachiomastus and P. simulans, and the COI sequences of P. rectidactylus and P. quinquelineatus (comparative species from NCBI). Considering the morphological and ecological characteristics together, we assume that P. simulans and P. rectidactylus are subspecies of P. brachiomastus and P. quinquelineatus, respectively. This study should facilitate further research on the taxonomic status of these species.

New record of two cymothoid isopods (Crustacea: Malacostraca: Isopoda) from South Korea

  • Song, Ji-Hun;Min, Gi-Sik
    • Journal of Species Research
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    • 제5권3호
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    • pp.313-317
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    • 2016
  • Two marine isopods, Elaphognathia sugashimaensis (Nunomura, 1981) and Metacirolana shijikiensis Nunomura, 2008, have been reported for the first time in South Korea. Specimens of E. sugashimaensis and M. shijikiensis were collected using light traps from Yeongdeok-gun and Gageo-do Island in South Korea, respectively. The genera Elaphognathia Monod, 1926 and Metacirolana Kussakin, 1979 are new to South Korea. In this paper, we provide descriptions of diagnostic characteristics and illustrations of their morphologies. Additionally, the partial sequences of mitochondrial cytochrome c oxidase subunit 1 (CO1) of two species are provided as molecular characteristics.

First report of the branchiobdellidan Holtodrilus truncatus (Annelida: Clitellata) found on the freshwater atyid shrimp Neocaridina sp. from Korea

  • Ahn, Dong-Ha;Min, Gi-Sik
    • Journal of Species Research
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    • 제5권3호
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    • pp.459-462
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    • 2016
  • The branchiobdellidan species Holtodrilus truncatus (Liang, 1963) is reported for the first time in Korea. The genus Holtodrilus Gelder and Brinkhurst, 1990 is monotypic within the family Xironodrilidae, and the genus and family of this species are also new to the Korean fauna. The branchiobdellidan specimens were collected from the exoskeletons of the freshwater atyid shrimp, Neocaridina sp., sampled from the 'Andeok Valley', Jeju-do Province, South Korea. Holtodrilus truncatus is characterized by its terete body form, 7/7 dental formula (with a large medial tooth and three pairs of smaller lateral teeth), and no trunk appendages. In addition, we determined a partial sequence of the mitochondrial cytochrome c oxidase subunit 1 (CO1) gene as a DNA barcode marker for H. truncatus. The present study is the first record of the branchiobdellidan occurrence on a non-crayfish host (shrimp) in Korea. To date, seven species (four genera in three families) of branchiobdellidans have been reported in the Korean fauna.

New record of limnoriid and asellote species(Crustacea: Malacostraca: Isopoda) from South Korea

  • Song, Ji-Hun;Min, Gi-Sik
    • Journal of Species Research
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    • 제6권3호
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    • pp.237-240
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    • 2017
  • During a scientific survey of Korean isopod fauna, we discovered two marine isopods, Limnoria segnoides Menzies, 1957 and Munna japonica Shimomura and Mawatari, 2001, which are new records to South Korea. Limnoria segnoides and M. japonica were collected by SCUBA diving from the subtidal zone of Jeju Island and Yangyang-gun in South Korea, respectively. In this paper, we provide diagnosis and illustrations of the two isopods. Additionally, we provide GenBank accession numbers of partial sequences of mitochondrial cytochrome c oxidase subunit 1 (CO1) of two species.