• Journal of the Korean Wood Science and Technology
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• 제38권2호
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• pp.135-139
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• 2010

Reticulitermes speratus is commonly found in Asia, including Korea and Japan. We recently analyzed the 5' region of mitochondrial cytochrome c oxidase subunit I to perform a phylogenetic analysis of R. speratus KMT1, isolated in Seoul, Korea. Our results, using COXI, suggest that the taxonomy of R. speratus should be reconsidered with regard to the subgenus group. A similar phylogenetic analysis by COXI and COXII demonstrated the reliability of COXI genetic information in a molecular phylogenetic analysis of termites.

• Journal of Species Research
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• 제3권1호
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• pp.27-34
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• 2014

Two species of Tortanus (Eutortanus) are newly recorded from shallow Korean waters: T. (E.) vermiculus Shen, 1955 and T. (E.) komachi Itoh, Ohtsuka and Sato, 2001. As a result of this study, five species are reported in the subgenus of the family Tortanidae in Korea. The sequence of cytochrome c oxidase subunit 1 (CO1) is also provided as a molecular characteristic.

• Animal cells and systems
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• 제13권2호
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• pp.105-112
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• 2009

In this study, the effects of iron on cytochrome c oxidase (CcO) in rat lung mitochondria were examined. Similar to liver mitochondria, iron accumulated considerably in lung mitochondria (more than 2-fold). Likewise, the reactive oxygen species and nitric oxide (NO) content of mitochondria were increased by more than 50% and 100%, respectively. NO might be produced by nitric oxide synthase (NOS), eNOS and iNOS type, with particular contribution by NOS in mitochondria. The respiratory control ratio of iron overloaded lung mitochondria dropped to nearly 50% due to increased state 4. Likewise, cytochrome c oxidase activity was lowered significantly to approximately 50% due to excess iron. Real-time PCR revealed that the expression of isoforms 1 and 2 of subunit IV of CeO was enhanced greatly under excess iron conditions. Taken together, these results show that oxidative phosphorylation within lung mitochondria may be influenced by iron overload through changes in cytochrome c oxidase and NO.

• Animal Systematics, Evolution and Diversity
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• 제27권1호
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• pp.35-46
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• 2011

Three aetideid copepods collected from the East Sea of Korea are described: Bradyidius angustus (Tanaka, 1957), Gaetanus minutus (Sars, 1907), and Aetideus acutus Farran, 1929. The former two species are new to the Korean copepod fauna. The sequences of cytochrome c oxidase subunit 1 were determined to be the molecular characteristics of these three species.

• Parasites, Hosts and Diseases
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• 제45권3호
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• pp.181-190
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• 2007

The phylogenie relationships existing among 14 parasitic Platyhelminthes in the Republic of Korea were investigated via the use of the partial 28S ribosomal DNA (rDNA) D1 region and the partial mitochondrial cytochrome c oxidase subunit 1 (mCOI) DNA sequences. The nucleotide sequences were analyzed by length, G + C %, nucleotide differences and gaps in order to determine the analyzed phylogenie relationships. The phylogenie patterns of the 28S rDNA D1 and mCOI regions were closely related within the same class and order as analyzed by the PAUP 4.0 program, with the exception of a few species. These findings indicate that the 28S rDNA gene sequence is more highly conserved than are the mCOI gene sequences. The 28S rDNA gene may prove useful in studies of the systematics and population genetic structures of parasitic Platyhelminthes.

• Animal Systematics, Evolution and Diversity
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• 제28권4호
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• pp.269-278
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• 2012

Gobiids are hyperdiverse compared with other teleost groups, with about 2,000 species occurring in marine, freshwater, and blackish habitats, and they show a remarkable variety of morphologies and ecology. Testing the effectiveness of DNA barcodes on species that have emerged as a result of radiation remains a major challenge in evolutionary biology. Here, we used the cytochrome c oxidase subunit 1 (COI) sequences from 144 species of gobies and related species to evaluate the performance of distance-based DNA barcoding and to conduct a phylogenetic analysis. The average intra-genus genetic distance was considerably higher than that obtained in previous studies. Additionally, the interspecific divergence at higher taxonomic levels was not significantly different from that at the intragenus level, suggesting that congeneric gobies possess substantial interspecific sequence divergence in their COI gene. However, levels of intragenus divergence varied greatly among genera, and we do not provide sufficient evidence for using COI for cryptic species delimitation. Significantly more nucleotide changes were observed at the third codon position than that at the first and the second codons, revealing that extensive variation in COI reflects synonymous changes and little protein level variation. Despite clear signatures in several genera, the COI sequences did resolve genealogical relationships in the phylogenetic analysis well. Our results support the validity of COI barcoding for gobiid species identification, but the utilization of more gene regions will assist to offer a more robust gobiid species phylogeny.

• 생명과학회지
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• 제26권4호
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• pp.406-413
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• 2016

전복은 전복속(Haliotis)에 속하며 전 세계적으로 식품산업에서 중요한 복족류 연체동물이다. 우리나라에는 6개종; 북방전복(Haliotis discus hannai), 둥근전복(Haliotis discus discus), 왕전복(Haliotis madaka), 말전복(Haliotis gigantea), 오분자기(Haliotis diversicolor diversicolor), 마대오분자기(Haliotis diversicolor supertexta)가 보고되어 있다. 이 연구에서는 우리나라 해역에 서식하는 중ㆍ대형 전복과 4종인 북방전복, 둥근전복, 왕전복, 말전복의 유전학적 유연관계를 분석하기 위하여 미토콘드리아의 cytochrome c oxidase subunit I (COI) 유전자와 Random Amplified Polymorphic DNA (RAPD) 분석법을 실시 하였다. 본 연구의 결과 COI 유전자 분석과 RAPD 분석을 활용하면 4종의 전복 중 북방전복, 둥근전복, 왕전복을 한 그룹으로 나머지 한 그룹을 말전복으로 구분하는 종 분류는 명확히 구분할 수 있었다. 이러한 결과는 전복 교잡육종을 이용한 수출용 전복 신종자 개발에 있어 주요 대상종인 전복과 4종에 대한 유전적 근연 관계를 규정함으로써 향후 교잡육종 연구의 기초 자료를 제공할 수 있을 것으로 사료된다.

• ALGAE
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• 제33권1호
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• pp.49-54
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• 2018

Red algal mitochondrial genomes (mtDNAs) can provide useful information on species identification. mtDNAs of Pyropia / Porphyra (Bangiales, Rhodophyta) have shown diverse variation in their size and gene structure. In particular, the introns and intronic open reading frames found in the ribosomal RNA large subunit gene (rnl) and cytochrome c oxidase subunit 1 gene (cox1) significantly vary the mitochondrial genome size in Pyropia / Porphyra species. In this study, we examined the exon / intron structure of rnl and cox1 genes of Pyropia yezoensis at the intraspecific level. The combined data of rnl and cox1 genes exhibited 12 genotypes for 40 P. yezoensis strains, based on the existence of introns. These genotypes were more effective to identify P. yezoensis strains in comparison to the traditional DNA barcode cox1 marker (5 haplotypes). Therefore, the variation in gene structure of rnl and cox1 can be a novel molecular marker to discriminate the strains of Pyropia species.

• Parasites, Hosts and Diseases
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• 제42권2호
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• pp.71-75
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• 2004

To determine the molecular phylogenie location of Plagiorchis muris, 28S D1 ribosomal DNA (rDNA) and mitochondrial cytochrome C oxidase subunit I (mtCOI) were sequenced and compared with other trematodes in the family Plagiorchiidae. The 28S D1 tree of P. muris was found to be closely related to those of P. elegans and other Plagiorchis species. And, the mtCOI tree also showed that P. muris is in a separate clade with genus Glypthelmins. These results support a phylogenie relationship between members of the Plagiorchiidae, as suggested by morphologic features.

• Animal Systematics, Evolution and Diversity
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• 제35권3호
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• pp.136-139
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• 2019

The pulmonate gastropod Ellobium chinense (Pfeiffer, 1864) is an endangered marine species along the South Korean coasts due to habitat destruction and population declines. We sequenced the cytochrome c oxidase subunit 1 (COI) of 25 E. chinense specimens collected from five coastal sites in South Korea, and identified 16 unique haplotypes. The maximum intraspecific variation among individuals was 1.6%, while interspecific differences from another ellobiid species, Auriculastra duplicata (Pfeiffer, 1854), ranged from 21.9 to 23.0%. Our barcoding data will be useful to elucidate the phylogenetic relationships among pulmonate gastropods and infer the population genetic structure of E. chinense.