• Title/Summary/Keyword: Cystine

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우모를 이용한 taurine 강화 계란 생산

  • 이승민;임희석;백인기
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2003.11a
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    • pp.119-120
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    • 2003
  • The experiments were conducted to investigate the effects of supplementary feather meal(FM) and its digest on the taurine content and performance of laying hens. Feeding trial was conducted with 810 78-wk-old ISA-Brown layers for 5wk. The experiment consisted of nine dietary treatments ; T1 ; control(basal diet), T2;Tl + feather meal(FM) 6 % diet, T3;T2 + pyridoxin(21 mg/kg) supplemented diet, T4; T1 + H$_2$O$_2$treated FM 6%, T5; T4 + pyridoxin(21 mg/kg) supplemented diet, T6; T1 + cystine(0.25 %) supplemented diet, T7; T6 + pyridoxin(21 mg/kg) supplemented diet, T8; T1 + synthetic taurine supplemented(0.25%) diet, T9; T1 + synthetic taurine supplemented(0.5 %) diet. Diets were formulated to be iso-caloric and to meet NRC(1994) requirements of essential nutriente. Birds in treatments T3 showed significantly higher(P<0.01) egg production, feed intake, feed conversion ratio, egg weight, haugh unit and eggshell strength than those fed other treatments. Supplementation of FM. H$_2$O$_2$treated FM and cystine with or without pyridoxin did not significantly affected taurine content of egg yolk. Only synthetic taurine supplemented diets(T8 and T9) linearly increased(P<0.01) taurine content of egg yolk.

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Effect of De-hulling on Ileal Amino Acids Digestibility of Soybean Meals Fed to Growing Pigs

  • Kang, Y.F.;Li, D.F.;Xing, J.J.;Mckinnon, P.J.;Sun, D.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.6
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    • pp.928-939
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    • 2003
  • A study was carried out to determine the effect of de-hulling on apparent and true ileal amino acids digestibility of soybean meals for growing pigs. Twenty barrows (Duroc${\times}$Large white${\times}$Longer white) were fitted with a simple T-cannula at the distal ilium. Digestibility of 20 experimental diets was determined, nine of them were de-hulled soybean meal diets, and nine of them were regular soybean meal diets and two low protein casein diets for determination of endogenous amino acid correction for true digestibility determination. A TEX>$5<{\times}5<$ Latin Squares Design was adopted in this trail. The results showed that de-hulling increased apparent ileal digestibility of isoleucine, threonine, aspartic, tyrosine and indispensable and dispensable amino acid (p<0.05) in soybean meals. Furthermore, dehulling is also increased apparent digestibility of arginine, leucine, lysine, phenylalanine, alanine, glutamic acid, serine and gross amino acids (p<0.01). However, there were no significant differences found for histidine, methionine, tryptophan, cystine and glycine (p>0.05). Similar responses were found for true ileal digestibility. In three dehulled and non-dehulled pairs soybean meals from the same respective sources, de-hulling increased apparent digestibility of lysine, methionine, threonine and cystine 1.42%, 2.06%, 2.18% and 1.40% respectively. True digestibility of lysine, methionine, threonine and cystine was increased 1.65%, 1.94%, 2.30% and 1.82% respectively. A prediction equation for true ileal amino acid digestibility (including lysine and arginine) was established by multivariate linear regression. The independent variables included relevant amino acid, organic matter, crude protein, ether extract and nitrogen free extract. The coefficient R2 values of lysine and agrinine were 0.596 and 0.531 respectively. According to the crude protein content, a prediction equation for lysine and arginine content in soybean meal was also established by single linear regression. The coefficient $R^2$ values of lysine and agrinine were 0.636 and 0.636 respectively.

Organic Constituents in Kimchis (Ixeris sonchifolia H.) -On free amino acids- (고들빼기 김치의 유기성분(有機成分)(I) - 유이(遊離)아미노산(酸)에 관(關)하여 -)

  • Kang, Dong Hee;Woo, Young Sook;Lee, Young Kyoung;Chung, Seung Yong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.12 no.3
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    • pp.225-229
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    • 1983
  • The change of free amino acids was identified during fermentation of kimchis (Ixeris sonchifoliaH.) added fermented anchovy. The results were summarized as follows; In fresh roots and leaves of Ixeris sonchifolia H., 8 kinds of free amino were determined respectively. Among them, argine, cysteine and glutamic acid were abundant in fresh roots, while arginine, valine, isoleucine and phenylalanine in fresh leaves, especially arginine was dominant in fresh both roots and leaves. The amount of total amino acids in fresh leaves was about 2.5 times of that of roots. After fermentation, 15 kinds of free amino acids were determined in kimchis, and the characteristic favor of in was attributed to such amino acids as threonine, glutamic acid, alanine, leucine and cysteine. The content of total free amino acids in kimchi leaves was increased to about 5 times of that in fresh (9,435,6mg % on dry base), but in kimchi roots, 11 times of that in fresh was contained (7,079,1mg % on dry base) In kimchi'es extract, 16 kinds of free amino acids were determined, and threonine, glutamic acid, alanine, cysteine and leucine were abundant.

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A Case of Cystinuria with Multiple Renal Stones in an 8-month-old Girl (8개월 여아에서 다발성 결석으로 진단된 시스틴뇨증 1례)

  • Kang, Eun Gu;Lee, Joo Hoon;Lee, Beom Hee;Kim, Gu-Hwan;Park, Young Seo
    • Childhood Kidney Diseases
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    • v.17 no.2
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    • pp.122-126
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    • 2013
  • Cystinuria is an autosomal recessive disease characterized by impaired transport of cystine and dibasic amino acids in the proximal renal tubule, resulting in the formation of cystine stones. It is believed to account for about 1% of all kidney stones and up to 10% of pediatric stones. Here we report a case of cystinuria with multiple renal stones confirmed by genetic mutational analysis. An 8-month-old girl was admitted to AMC with persistent fever and multiple renal stones. A renal sonogram showed multiple stones at the right renal pelvis, right distal ureter, and left renal medullary portion. An approximately 1 cm renal stone was extracted spontaneously, and stone analysis revealed it to be composed entirely of cystine. Cystinuria was confirmed by increased urine dibasic amino acid levels, including cysteine, and genetic mutational analysis showed the patient to be a homozygote for the pathogenic c. 1820del (p.L607fs) of SLC3A1. Despite treatment with oral hydration and urinary alkalinization, and restricted intake of animal protein, the stones increased in size and number. The patient has since been treated with tiopronin.

Studies on the Constitutents of the Leaves of Perilla frutescens $B_{RITTON}$ -Identification of Free Amino Acids- (들깨잎의 성분연구 -유리 Amino산의 분석-)

  • Kim, Tae-Hee
    • Korean Journal of Pharmacognosy
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    • v.2 no.4
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    • pp.173-175
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    • 1971
  • By means of the two-dimensional thin-layer chromatography, free amino acid components of the leaves of $Perilla\;frutescens\;B_{RITTON}$ have been examined and twelve spots have been detected by the coloration with ninbydrin reagent. These were found to be aspartic acid, asparagine, alanine, phenylalanine, tryptophan, leucine, glutamic acid, proline, threonine, histidine, cystine, and glycine, tentatively.

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Cystinuria in Siblings (남매에서 발생한 Cystinuria)

  • Lee, Dong Hwan
    • Journal of The Korean Society of Inherited Metabolic disease
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    • v.1 no.1
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    • pp.18-22
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    • 2001
  • Renal colic, hematuria, dysuria and stone passage were developed in younger brother (4 year 6 month old boy). But the elder sister (6 year old girl)had no specific symptoms and signs. The identification of the disease was proved by cyanide nitroprusside test and amino acid analysis of urine. In our patients the chromatographic amino acid patterns of urine showed remarkably increased excretion of cystine, ornithine, lysine, and arginine. They are managed by adequate hydration with Shohl solution for rendering the urine more alkaline, and alpha-mercaptopropionylglycine (Thiola).

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Quality Indexes for the Evaluation of Cigarette Smoke. (담배연기의 평가를 위한 새로운 계수)

  • 신영국;노동석;유광근
    • Journal of the Korean Society of Tobacco Science
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    • v.3 no.2
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    • pp.123-128
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    • 1981
  • For evaluating the cigarette smoke quality of nonblended and commercial cigarettes, four modified smoke quality indexes were deduced from considering the reactivity of the thiol group in cystine (SH value) with the cigarette smoke constituents, and from the maximum limited contents of tar. nicotine, and carbon monoxide for the less harmful cigarettes. These results were discussed with respect to correlation coefficients between the indexes and tar/nicotine ratio which had been known reliable for smoke quality evaluation. Among the three indexes, the SH/[(N/O.6) +($\frac{C+T}{8}$)] index was found to be the most reliable and applicable.

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On the Sulfur-incorporation into amino acids in germinating Soy Beam seeds using Sulfate-$S^35$ (대두발아종자에 있어서 아미노산에의 유황결합에 관하여)

  • 이민재
    • Journal of Plant Biology
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    • v.8 no.3
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    • pp.1-3
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    • 1965
  • The sulfur-incorporation into amino acids in germinating Soy Bean seeds was studied with the aid of Sulfate-S35. Water imbibition and isotope absorption were almostly pararelled. On the radioautogram $S^35$ was detected in cysteic acids at 90 minutes. Cysteine, cystine and cysteic acid were appeared with radioactivity at 240 minutes after the infiltration of sulfate-$S^35$. Methione was not detected on the radioautogram in the range of 300 minutes. The radioactivity of $S^35$ was detected highly in free amino acids than hydrolyzates.

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Ox-Red Cell Membrane 抗原의 免疫 反應 硏究: I. 抗原成分의 分析實驗

  • 한국동물학회
    • The Korean Journal of Zoology
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    • v.15 no.2
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    • pp.92-93
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    • 1972
  • Ox-RBC로부터 Moddy法에 依하여 Ox-RCM抗原을 抽出하여 몇가지 分析을 實施하였다. Lyophilized 한 Ox-RCM protein의 化學的 分析 結果 total protein은 $55\\pm 1.3%$, total nitrogen은 9.2%, carbohydrate 8.5% 그리고 total acid soluble phosphorus는 $0.96\\pm 0.1%$였다. Polyacrylamide gel electrophoresis한 結果 Ox-RCM은 12 $\\sim$ 15個, Pre-VRS fraction은 8個 및 PHR 分劃은 9個의 pattern을 나타냈다. Amino acid의 分析 結果로 cystine이 4.93 $\\sim$ 3.72 moles/100 moles 이고 glycine이 最高로 26.7 $\\sim$ 13.5 moles/100 moles였고 最低로 methionine 이었으나 試料의 抽出方法에 따라 그 値의 變動을 볼 수 있다.

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Effect of Certain Additives on Bread-Making Quality of Wheat-Purple Sweet Potato Flours (밀 및 자색고구마 가루의 제빵성에 대한 첨가제의 영향)

  • 김선영;유정희
    • Korean journal of food and cookery science
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    • v.13 no.4
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    • pp.492-499
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    • 1997
  • The effect of oxidants, gluten and gums on breadmaking quality of wheat-purple sweet potato(Ipomoea batatas) composite flour were studied. Alveograph tests of doughs showed that all additives increased the P, L and W values. Of these additives, L-Ascorbic acid, gluten, carboxy methyl cellulose have a significant effect on alveogram indexes. SEM showed that the dough added with additives changed some what in appearance when compared with the control. When oxidants was added, the doughs discontinuous gluten film were observed. No significant differences ,were evident in bread crumb color among the additives. And textural characteristics of bread crumb were influened by adding additives. Breads containing additives showed an increase in max weight, strength and hardness and a decrease in springness and cohesiveness. Average enthalpy values for all bread crumb after 7days increased with storage time. However, addition of additives decrease the rate of staling slightly more than that of the control. Addition of L-Ascorbic acid, L-Cystine, carboxy methyl cellulose and hydroxy propyl methyl cellulose increased the loaf volume significantly, and with except potassium bromate bread scores were acceptable.

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