• 제목/요약/키워드: Cynodon

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Whole Genome Sequencing and Gene Prediction of Cynodon transvaalensis

  • Sol Ji Lee;Chang soo Kim
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2022년도 추계학술대회
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    • pp.237-237
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    • 2022
  • Cynodon transvaalensis belongs to the warm-season grasses and is one of the economically and ecologically important crops. Cynodon species with high heterozygosity are difficult to assemble, so genome research has not been actively conducted. In this study, hybrid assembly was performed by sequencing with Illumina and PacBio. As a result of the assembly, the number of scaffolds and the length of N50 were 1,392, 928 kb, respectively. The completeness of the assembly was confirmed by BSUCO at 98.3%. In addition, as a result of estimating the size of the assembled genome by K-mer analysis (k=25), it was approximately ~413 Mb. A total of 37,060 cds sequences were annotated in the assembled genome, and their functions were identified through blast. After that, we try to complete the assembled genome into a pseudochromosome-level genome through Hi-C technology. These results will not only help to understand the complex genome composition of african bermudagrass, but also provide a resource for genomic and evolutionary studies of grass and other plant species.

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Performance of Three Warm Season Turfgrasses under Linear Gradient Irrigation

  • Ow, Lai Fern;Ghosh, Subhadip
    • Weed & Turfgrass Science
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    • 제6권1호
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    • pp.61-66
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    • 2017
  • The appropriate level of irrigation for turfgrasses is vital to the performance of the turfgrass as well as conservation of water. Linear gradient irrigation system (LGIS) facilitates long-term study of turf performance under continuous irrigation gradients at extreme ends of the irrigation scale. The objectives of this study were to: a) determine the minimum irrigation requirements and relative drought resistance in three warm season turfgrasses; and b) evaluate the medium to long-term effects of irrigation levels on turf persistence, weed invasion, and susceptibility to diseases. Results suggest that grasses differed in drought resistance and persistence under variable irrigation regimes. Irrigation (Ep) required for consistent acceptable turf quality for respective grasses was Cynodon dactylon x C. transvaalensis (61%), Zoysia matrella L. Merr (73%), and Stenotaphrum secundatum 'Palmetto' (86%). Brown patch infection was most prevalent in Stenotaphrum secundatum 'Palmetto' at 12 and 125% Ep irrigation. Cynodon dactylon x C. transvaalensis and Zoysia matrella L. Merr were better able to adapt to the various irrigation regimes, and this ability allowed these species to resist drought, and maintain turf coverage which in turn, kept weeds and the occurrence of diseases at bay. Ranking these grasses for their drought tolerance abilities showed that Cynodon dactylon x C. transvaalensis had the most outstanding resistance against drought, followed by Zoysia matrella L. Merr, and lastly, Stenotaphrum secundatum 'Palmetto'. Despite having the highest irrigation requirement, Stenotaphrum secundatum 'Palmetto' was still not able to maintain persistence at high irrigation regimes. Likewise, this grass also lost turf coverage at low irrigation levels.

난지형 잔디와 한지형 잔디의 혼식에 관한 생태학적 연구 (Ecological Studies on the Warm-Season Turfgrass and Cool-Season Turfgrass Mixtures)

  • 박봉주
    • 한국환경복원기술학회지
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    • 제6권5호
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    • pp.21-27
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    • 2003
  • 본 연구는 난지형 잔디와 한지형 잔디의 혼식구의 5년간에 걸친 초종 구성비율의 경년변화를 조사하여 안정성이 높은 초종 품종의 조합을 밝히고 시비법이 난지형 잔디와 한지형 잔디 초종의 구성비율에 미치는 영향을 검토하였다. 한지형 잔디를 혼식하여 5년째인 시점에서 난지형 잔디와 한지형 잔디 혼식구의 초종 구성비율은 센티페드그래스와 버팔로그래스와의 혼식구를 제외하고는 들잔디, 세인트어거스틴그래스, 버뮤다그래스는 대체적으로 반반 정도의 비교적 안정된 혼식비율을 유지하고 있었다. 이들 초종들은 난지형 잔디와 한지형 잔디의 혼식을 위한 이용적성이 높은 것으로 사료된다. 그러나, 센티페드그래스와의 혼식구에서는 센티페드그래스가 버팔로그래스와의 혼식구에서는 한지형 잔디가 우점하여 이들 난지형 잔디는 한지형 잔디와의 혼식에 적합하지 않은 것으로 나타났다. 난지형 잔디와 한지형 잔디 혼식구의 시비는 난지형 잔디가 휴면중인 시기에 실시하면 한지형 잔디가 우점하게 되는 경향을 보여 난지형 잔디의 생육기인 4~8월 사이에 실시하는 것이 바람직한 것으로 나타났다. 이들 난지형 잔디와 한지형 잔디 혼식구는 장기간에 걸쳐 안정적으로 일정한 초종 구성비율이 유지되는 것이 중요하기 때문에 앞으로도 계속적인 조사가 이루어져야 할 것으로 사료된다.

골프장의 신 해충, 고구마뿌리혹선충의 발견 (A Newly Recorded Turfgrass Pest, Root-knot Nematode, Meloidogyne incognita, in Korean Golf Courses)

  • 추호렬;이동운;김형환;박지웅;성영탁;정영기
    • 아시안잔디학회지
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    • 제12권2호
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    • pp.107-112
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    • 1998
  • 우리나라 골프장의 잔디 가해 해충과 천적을 조사하던 중 농림작물의 중요해충인 고구마뿌리혹선충을 Zoysia잔디에서 발견하였다. 고구마뿌리혹선충에 감염된 잔디는 새 뿌리가 발육하지 못하고 뭉툭하게 되어 황화현상과 함께 전반적으로 생육이 부진하였다. 감염된 잔디의 종은 들잔디(Zoyais japonica)와 금잔디(Zoysia matrella), 버뮤다글라스(Cynodon sp.)였다. 그리고 Z. japonica는 고구마뿌리혹선충의 새로운 기주로 기록된다. 조사 골프장의 대부분이 정도의 차이는 있었지만 피해를 받고 있었다. 잔디에서 뿌리혹선충이 기록되기는 우리나라에서는 처음이다.

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국내에서 수집된 버뮤다그래스의 입자환경 및 형태적 특성 (Site Environment and Morphology Characteristics of Native Bermudagrass (Cynodon spp.) Ecotypes in Korea)

  • 배은지;이광수;박남창;이상명;신현철;양근모
    • 아시안잔디학회지
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    • 제25권1호
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    • pp.11-16
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    • 2011
  • 본 연구는 국내 해안 및 도서지역에 자생하고 있는 버뮤다그래스를 지역별로 수집하여 입지환경에 따른 형태적 변이 정도를 알아보고자 수행되었다. 이들 입지환경, 토양의 화학성과 형태적 특성을 조사하였다. 수집된 자생지에 따라 초장, 엽폭, 엽장, 잎털, 포복경 및 화수당 종자수 등에서 큰 변이를 보여 조사 형질 간에 다양한 변이를 보이는 것을 확인할 수 있었다. 광엽과 세엽 특성의 버뮤다그래스가 존재하고 있었으며, 광엽형의 버뮤다그래스의 경우 열악한 환경에서의 적응성이 매우 높아 중 저관리의 잔디면에 이용 가치가 높을 것으로 생각된다. 상기 입지 환경과 형태적 특성 조사 결과 및 수집된 유전자원들은 앞으로 유전자원으로서 보존 가치가 높으며, 유용유전자를 보존할 수 있는 체계가 마련되어야 할 것으로 판단된다.

Mineral Concentration in Blood of Grazing Goats and Some Forage in Lahar-Laden Area of Central Luzon, Philippines

  • Orden, E.A.;Serra, A.B.;Serra, S.D.;Aganon, C.P.;Cruz, E.M.;Cruz, L.C.;Fujihara, T.
    • Asian-Australasian Journal of Animal Sciences
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    • 제12권3호
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    • pp.422-428
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    • 1999
  • The mineral status of native goats and forage species, namely; Cynodon plectostachyus, Pennisetum purpureum. Eleusine indica, Cynodon dactylon, Calopogonium muconoides, Centrosema pubescens, Leucaena leococephala, and Mimosa pudica in lahar affected areas of Concepcion, Tarlac, Philippines were determined. Forage and blood samples were collected six times in 1996-97, and analyzed for calcium, phosphorus, magnesium, sulfur, copper, iron, molybdenum, selenium, and zinc. Forage calcium and sulfur are non-limiting. Most species had low phosphorus, copper and selenium, while some had magnesium and zinc levels lower than the critical limit because of low mineral content and high percolation rate of lahar deposits. Iron and molybdenum were in excess. The effect of seasonal variation was observed only in copper, sulfur and iron. Average blood mineral concentration of the animals was above critical limit, but there were no significant differences between seasons. All the animals had plasma phosphorus and magnesium above critical level; but 20 % had low copper, zinc and selenium especially in dry season possibly due to insufficient amount of these elements and excessive molybdenum and iron in most forage. Conversely, calcium in forage was high; but 40 % of the animals had low plasma calcium concentration. Although no clinical signs of mineral deficiencies were observed, supplemental feeding would be important since the condition of the pasture in lahar-laden areas is not expected to improve in the next five years. Intensified use of L. leucocephala with better mineral profile would be ideal.

Genetic Diversity among Korean Bermudagrass (Cynodon spp.) Ecotypes Characterized by Morphological, Cytological and Molecular Approaches

  • Kang, Si-Yong;Lee, Geung-Joo;Lim, Ki Byung;Lee, Hye Jung;Park, In Sook;Chung, Sung Jin;Kim, Jin-Baek;Kim, Dong Sub;Rhee, Hye Kyung
    • Molecules and Cells
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    • 제25권2호
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    • pp.163-171
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    • 2008
  • The genus Cynodon comprises ten species. The objective of this study was to evaluate the genetic diversity of Korean bermudagrasses at the morphological, cytological and molecular levels. Morphological parameters, the nuclear DNA content and ploidy levels were observed in 43 bermudagrass ecotypes. AFLP markers were evaluated to define the genetic diversity, and chromosome counts were made to confirm the inferred cytotypes. Nuclear DNA contents were in the ranges 1.42-1.56, 1.94-2.19, 2.54, and 2.77-2.85 pg/2C for the triploid, tetraploid, pentaploid, and hexaploid accessions, respectively. The inferred cytotypes were triploid (2n = 3x = 27), tetraploid (2n = 4x = 36), pentaploid (2n = 5x = 45), and hexaploid (2n = 6x = 54), but the majority of the collections were tetraploid (81%). Mitotic chromosome counts verified the corresponding ploidy levels. The fast growing fine-textured ecotypes had lower ploidy levels, while the pentaploids and hexaploids were coarse types. The genetic similarity ranged from 0.42 to 0.94 with an average of 0.64. UPGMA cluster analysis and principle coordinate analysis separated the ecotypes into 6 distinct groups. The genetic similarity suggests natural hybridization between the different cytotypes, which could be useful resources for future breeding and genetic studies.

Study on Nutritive Value of Tropical Forages in North Sumatra, Indonesia

  • Evitayani, Evitayani;Warly, L.;Fariani, A.;Ichinohe, T.;Fujihara, T.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권11호
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    • pp.1518-1523
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    • 2004
  • This study was conducted to evaluate nutritive value of forages commonly used as ruminant feeds in North Sumatra, Indonesia. Seven species of grasses and five species of legumes were collected during the rainy season. The results showed that chemical composition, in vitro digestibility of dry matter (DMD), organic matter (OMD) and crude protein (CPD), in vitro gas production and metabolizable energy (ME) content greatly varied among the species of grass and legume forages. The CP content ranged from 6.6 to 16.2% in grass and from 17.5 to 29.1% in legumes; while NDF content of grass and legume ranged from 57.2 to 66.2% and from 24.4 to 55.6%, respectively. The DMD, OMD and CPD of grass ranged from 49.1 to 62.2%, 51.9 to 64.4% and 50.5 to 60.3%; while in legumes the values ranged from 59.1 to 71.8%, 65.2 to 72.0% and 68.2 to 71.6%, respectively. The ME content of grass varied from 6.4 to 9.3 MJ/kg and from 6.5 to 8.3 MJ/kg for legumes. In general, within species of grass Cynodon plectostachyus contained higher CP but was lower in NDF that resulted in much higher digestibility; a similar result was also found in Leucaena leucocephala for the legumes. The two forages also contained much higher ME than the others. In conclusion, the nutritive value of forages in North Sumatra, Indonesia during the rainy season was relatively high as ruminant feed, with the best quality noted for Cynodon plectostachyus and Leucaena leucocephala.

MONTHLY NUTRIENT VARIATION OF PARAGRASS (Brachiaria mutica) AND STARGRASS (Cynodon plectostachyum) COLLECTED FROM PASTURES GRAZED BY GOATS

  • Serra, A.B.;Serra, S.D.;Fujihara, M.;Orden, E.A.;Cruz, L.C.;Ichinohe, T.;Fujihara, T.
    • Asian-Australasian Journal of Animal Sciences
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    • 제9권2호
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    • pp.203-210
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    • 1996
  • A 13-month study was conducted to determine the monthly variation of crude protein(CP), cell contents(CC), fiber fractions and mineral concentrations of paragrass [Brachiaria mutica (Forsk.) Stapf.] and stargrass (Cynodon plectostachyum Pilger) and to estimate the correlations among the nutrient fractions and climatic factors. The forage samples collected by hand plucking were found to contain CP and mineral concentrations. i.e. calcium(Ca), magnesium(Mg), potassium(K) and zinc(Zn) above the critical levels based on ruminant veeds. Monthly differences(p<0.05) were observed in all CP, CC, neutral detergent fiber(NDF), acid detergent fiber(ADF), hemicellulose(HE), cellulose(CE) and acid detergent lignin(ADL) between the two grass species. Monthly differences(p<0.05) were also observed in all concentrations of forage Ca, K, phosphorus(P), copper(Cu) and Zn except in Mg of both grasses and K of stargrass. Species differences(p<0.05) were observed in all nutrient fractions except Mg and K concentrations. Rainfall had positive correlations to CP, P(p<0.01) and CC(p<0.05); it had negative correlations to NDF(p<0.05). ADF, CE, Ca, Cu and Zn(p<0.01). Temperature, humidity and daylength had also some correlations to various nutrient fractions.