• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.237-237
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• 2022

Cynodon transvaalensis belongs to the warm-season grasses and is one of the economically and ecologically important crops. Cynodon species with high heterozygosity are difficult to assemble, so genome research has not been actively conducted. In this study, hybrid assembly was performed by sequencing with Illumina and PacBio. As a result of the assembly, the number of scaffolds and the length of N50 were 1,392, 928 kb, respectively. The completeness of the assembly was confirmed by BSUCO at 98.3%. In addition, as a result of estimating the size of the assembled genome by K-mer analysis (k=25), it was approximately ~413 Mb. A total of 37,060 cds sequences were annotated in the assembled genome, and their functions were identified through blast. After that, we try to complete the assembled genome into a pseudochromosome-level genome through Hi-C technology. These results will not only help to understand the complex genome composition of african bermudagrass, but also provide a resource for genomic and evolutionary studies of grass and other plant species.

• Weed & Turfgrass Science
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• v.6 no.1
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• pp.61-66
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• 2017

The appropriate level of irrigation for turfgrasses is vital to the performance of the turfgrass as well as conservation of water. Linear gradient irrigation system (LGIS) facilitates long-term study of turf performance under continuous irrigation gradients at extreme ends of the irrigation scale. The objectives of this study were to: a) determine the minimum irrigation requirements and relative drought resistance in three warm season turfgrasses; and b) evaluate the medium to long-term effects of irrigation levels on turf persistence, weed invasion, and susceptibility to diseases. Results suggest that grasses differed in drought resistance and persistence under variable irrigation regimes. Irrigation (Ep) required for consistent acceptable turf quality for respective grasses was Cynodon dactylon x C. transvaalensis (61%), Zoysia matrella L. Merr (73%), and Stenotaphrum secundatum 'Palmetto' (86%). Brown patch infection was most prevalent in Stenotaphrum secundatum 'Palmetto' at 12 and 125% Ep irrigation. Cynodon dactylon x C. transvaalensis and Zoysia matrella L. Merr were better able to adapt to the various irrigation regimes, and this ability allowed these species to resist drought, and maintain turf coverage which in turn, kept weeds and the occurrence of diseases at bay. Ranking these grasses for their drought tolerance abilities showed that Cynodon dactylon x C. transvaalensis had the most outstanding resistance against drought, followed by Zoysia matrella L. Merr, and lastly, Stenotaphrum secundatum 'Palmetto'. Despite having the highest irrigation requirement, Stenotaphrum secundatum 'Palmetto' was still not able to maintain persistence at high irrigation regimes. Likewise, this grass also lost turf coverage at low irrigation levels.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.296-296
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• 2005

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.5
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• pp.21-27
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• 2003

Five warm-season turfgrass [Zoysia japonica Steud., Stenotaphrum secundatum (Walt.) Kuntze, Buchloe dactyloides (Nutt.) Engelm., Eremochloa ophiuroides (Munro.) Hack. and Cynodon dactylon (L.) Pers.] were overseeded with 2 cool-season turfgrasses [Poa pratensis L. and Festuca arundinacea Schreb.] to examine suitability of these species for planting in mixture for producing a year-round turf. Percent of warm-season and cool-season turfgrasses in the mixture were estimated for 5 years. Also, to evaluate the fertilization times of the warm-season and cool-season turrfgras mixtures. Eremochloa ophiuroides and Buchloe dactyloides were not suitable for warm-season and cool-season turfgrass mixtures. However, Zoysia japonica, Stenotaphrum secundatum and Cynodon dactylon were suitable for warm-season and cool-season turfgrass mixtures. Zoysia japonica, Stenotaphrum secundatum and Cynodon dactylon were showed approximately 50% botanical composition five years after cool-season turfgrass overseeding. And, those three warm-season turfgrass mixtures showed approximately 20% visual ratings in winter period. Fertilization in early spring and late autumn had made cool-season turfgrass dominated in mixtures. Therefore, fertilization times in warm-season and cool-season turfgrass mixtures was desirable in summer period.

• Asian Journal of Turfgrass Science
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• v.12 no.2
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• pp.107-112
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• 1998

우리나라 골프장의 잔디 가해 해충과 천적을 조사하던 중 농림작물의 중요해충인 고구마뿌리혹선충을 Zoysia잔디에서 발견하였다. 고구마뿌리혹선충에 감염된 잔디는 새 뿌리가 발육하지 못하고 뭉툭하게 되어 황화현상과 함께 전반적으로 생육이 부진하였다. 감염된 잔디의 종은 들잔디(Zoyais japonica)와 금잔디(Zoysia matrella), 버뮤다글라스(Cynodon sp.)였다. 그리고 Z. japonica는 고구마뿌리혹선충의 새로운 기주로 기록된다. 조사 골프장의 대부분이 정도의 차이는 있었지만 피해를 받고 있었다. 잔디에서 뿌리혹선충이 기록되기는 우리나라에서는 처음이다.

• Asian Journal of Turfgrass Science
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• v.25 no.1
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• pp.11-16
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• 2011

This study was conducted to identify the morphological characteristics and variations level by site environment of native bermudagrass (Cynodon spp.) from costal and island region in South Korea. Soil chemical properties and morphological characteristics were investigated. There were significant differences in plant height, leaf width, leaf length, cotton on leaf blade, stolon, and number of seed per spike depending on where they were collected from natural habitat. We have discovered a variety of variation among the investigated traits in Korean native bermudagrass ecotypes. There were broad leaf and fine leaf types of native bermudgarass in S. Korea. Adaptability was in very high on inadequate environment in bermudagrass with broad leaf types, it has also been suggested that management worth in terms of use. These results may provide basic information for bermudagrass breeding development and the collected types during this investigation would be worth being preserved as genetic resources for further breeding purposes.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.3
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• pp.422-428
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• 1999

The mineral status of native goats and forage species, namely; Cynodon plectostachyus, Pennisetum purpureum. Eleusine indica, Cynodon dactylon, Calopogonium muconoides, Centrosema pubescens, Leucaena leococephala, and Mimosa pudica in lahar affected areas of Concepcion, Tarlac, Philippines were determined. Forage and blood samples were collected six times in 1996-97, and analyzed for calcium, phosphorus, magnesium, sulfur, copper, iron, molybdenum, selenium, and zinc. Forage calcium and sulfur are non-limiting. Most species had low phosphorus, copper and selenium, while some had magnesium and zinc levels lower than the critical limit because of low mineral content and high percolation rate of lahar deposits. Iron and molybdenum were in excess. The effect of seasonal variation was observed only in copper, sulfur and iron. Average blood mineral concentration of the animals was above critical limit, but there were no significant differences between seasons. All the animals had plasma phosphorus and magnesium above critical level; but 20 % had low copper, zinc and selenium especially in dry season possibly due to insufficient amount of these elements and excessive molybdenum and iron in most forage. Conversely, calcium in forage was high; but 40 % of the animals had low plasma calcium concentration. Although no clinical signs of mineral deficiencies were observed, supplemental feeding would be important since the condition of the pasture in lahar-laden areas is not expected to improve in the next five years. Intensified use of L. leucocephala with better mineral profile would be ideal.

• Molecules and Cells
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• v.25 no.2
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• pp.163-171
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• 2008

The genus Cynodon comprises ten species. The objective of this study was to evaluate the genetic diversity of Korean bermudagrasses at the morphological, cytological and molecular levels. Morphological parameters, the nuclear DNA content and ploidy levels were observed in 43 bermudagrass ecotypes. AFLP markers were evaluated to define the genetic diversity, and chromosome counts were made to confirm the inferred cytotypes. Nuclear DNA contents were in the ranges 1.42-1.56, 1.94-2.19, 2.54, and 2.77-2.85 pg/2C for the triploid, tetraploid, pentaploid, and hexaploid accessions, respectively. The inferred cytotypes were triploid (2n = 3x = 27), tetraploid (2n = 4x = 36), pentaploid (2n = 5x = 45), and hexaploid (2n = 6x = 54), but the majority of the collections were tetraploid (81%). Mitotic chromosome counts verified the corresponding ploidy levels. The fast growing fine-textured ecotypes had lower ploidy levels, while the pentaploids and hexaploids were coarse types. The genetic similarity ranged from 0.42 to 0.94 with an average of 0.64. UPGMA cluster analysis and principle coordinate analysis separated the ecotypes into 6 distinct groups. The genetic similarity suggests natural hybridization between the different cytotypes, which could be useful resources for future breeding and genetic studies.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.11
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• pp.1518-1523
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• 2004

This study was conducted to evaluate nutritive value of forages commonly used as ruminant feeds in North Sumatra, Indonesia. Seven species of grasses and five species of legumes were collected during the rainy season. The results showed that chemical composition, in vitro digestibility of dry matter (DMD), organic matter (OMD) and crude protein (CPD), in vitro gas production and metabolizable energy (ME) content greatly varied among the species of grass and legume forages. The CP content ranged from 6.6 to 16.2% in grass and from 17.5 to 29.1% in legumes; while NDF content of grass and legume ranged from 57.2 to 66.2% and from 24.4 to 55.6%, respectively. The DMD, OMD and CPD of grass ranged from 49.1 to 62.2%, 51.9 to 64.4% and 50.5 to 60.3%; while in legumes the values ranged from 59.1 to 71.8%, 65.2 to 72.0% and 68.2 to 71.6%, respectively. The ME content of grass varied from 6.4 to 9.3 MJ/kg and from 6.5 to 8.3 MJ/kg for legumes. In general, within species of grass Cynodon plectostachyus contained higher CP but was lower in NDF that resulted in much higher digestibility; a similar result was also found in Leucaena leucocephala for the legumes. The two forages also contained much higher ME than the others. In conclusion, the nutritive value of forages in North Sumatra, Indonesia during the rainy season was relatively high as ruminant feed, with the best quality noted for Cynodon plectostachyus and Leucaena leucocephala.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.2
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• pp.203-210
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• 1996

A 13-month study was conducted to determine the monthly variation of crude protein(CP), cell contents(CC), fiber fractions and mineral concentrations of paragrass [Brachiaria mutica (Forsk.) Stapf.] and stargrass (Cynodon plectostachyum Pilger) and to estimate the correlations among the nutrient fractions and climatic factors. The forage samples collected by hand plucking were found to contain CP and mineral concentrations. i.e. calcium(Ca), magnesium(Mg), potassium(K) and zinc(Zn) above the critical levels based on ruminant veeds. Monthly differences(p<0.05) were observed in all CP, CC, neutral detergent fiber(NDF), acid detergent fiber(ADF), hemicellulose(HE), cellulose(CE) and acid detergent lignin(ADL) between the two grass species. Monthly differences(p<0.05) were also observed in all concentrations of forage Ca, K, phosphorus(P), copper(Cu) and Zn except in Mg of both grasses and K of stargrass. Species differences(p<0.05) were observed in all nutrient fractions except Mg and K concentrations. Rainfall had positive correlations to CP, P(p<0.01) and CC(p<0.05); it had negative correlations to NDF(p<0.05). ADF, CE, Ca, Cu and Zn(p<0.01). Temperature, humidity and daylength had also some correlations to various nutrient fractions.