• Title/Summary/Keyword: Cymbidium goeringii

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Ultrastructure of Cymbidium Leaf Tissue Systemically Infected with Odontoglossum Ringspot Virus (오돈토글로썸 윤문 바이러스에 감염된 Cymbidium 잎조직의 미세구조)

  • Ryu, Ki-Hyun;Kim, Woo-Kap;Park, Won-Mok
    • Applied Microscopy
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    • v.24 no.3
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    • pp.46-54
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    • 1994
  • In ultrathin section of the tissue of odontoglossum ringspot virus (ORSV)-infected Cymbidium goeringii Reichenbach, ORSV particles appeared as bundles of irregular aggregates of various length which were called stacked plates or rounded plates. Virus particles were found in the cytoplasm in electron clear zones and they also found between cell wall and plasma membrane. They mainly clustered in parallel aggregates and sometimes oriented randomly. The X-bodies and paramural bodies were observed near the cell membrane and these contained vacuole-like cavities. The cell wall of infected tissue expanded largely. Some chloroplast in ORSV infected cell was irregular. No virus particle was present in mitochondria, nuclei, vacuoles, vesicles or other organelles. The plasmodesmata slightly enlarged, and virus-associated granules were present around it.

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A Comparison of Functional Fragrant Components of Cymbidium (Oriental Orchid) Species (기능성 지표물질 확인을 위한 동양란 심비디움(Cymbidium) 향기 성분 비교 분석)

  • Kim, Sung Min;Jang, Eu Jean;Hong, Jong Won;Song, Sung Ho;Pak, Chun Ho
    • Horticultural Science & Technology
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    • v.34 no.2
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    • pp.331-341
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    • 2016
  • We analyzed the functional fragrant components of three species of Cymbidium oriental orchids using gas chromatography-mass spectrometry (GC/MS). For the comparative analysis, C. goeringii 'Minchunran', 'Jugeumhwa', C. forrestii 'Chwigae', 'Songmae', 'Yongja', and C. faberi 'Choemae', 'Namyangmae', 'Hwaja' were investigated. Major fragrant components detected by GC/MS were selected on the basis of more than 3% value according to the analysis of peak area (%). We found that ${\alpha}$-bergamotene, which has a cytotoxic effect on breast cancer, cervical cancer, and glioblastoma, and nerolidol, which induces apoptosis of human hepatoma cells (HepG2), inhibits the growth of Streptococcus mutans and babesiosis, and has antibacterial properties, are common substances produced by C. goeringii L. Nerolidol and ${\beta}$-bisabolene, which is cytotoxic and suppresses the growth of malignant melanoma cells (B16-F10), HepG2, and leukemia cells (HL-60, K562), are major substances in C. forrestii R. Furthermore, ${\alpha}$-pinene, which inhibits the growth of gliobastoma cells (SF-767) and inhibits the anti-inflammatory action of hepatoma cells (BEL-7402); 1,8-cineole, which is a potential therapeutic agent for the treatment of gastric ulcers; and 1,3,7-octatriene, which functions as a pheromone, are the most common substances in C. faveri R. Thus, substances identified as major fragrant components in oriental orchid species have multiple beneficial applications in human health. This research forms the basis for further studies of the roles of major fragrant components in oriental orchids.

Effects of NaOCl Treatment on Seed Germination and Capsule Formation of Level of Flowering in Cymbidium goeringii (춘란(Cymbidium goeringii)의 개화정도별 삭과 형성 및 종자 발아에 미치는 NaOCl의 영향)

  • Lee, Dae Gun;Koh, Jae Chul
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.4
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    • pp.271-277
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    • 2010
  • The study is aimed to obtain the basic data for developing new variations of wild spring orchid. The results was investigated the capsules' formational characteristics and the germination ratio after having been self-pollinated by dividing the flowering period into the 5 stages into budding time, semi-flowering, full-flowering, 10 days after flowering, and 20 days after flowering. The fruit setting ratio was the highest as 100% in the plant which had been pollinated 20 days after the flowering, while the weight of the capsule was heaviest in the orchid which had been pollinated in semi-flowering period. As the result of investigating the germination ratio by dividing the period into 5 stages, it was the highest in the plant which had been pollinated during the semi-flowering period, and in the result of investigating the germination ratio by dividing the seeds harvesting days into the 3 stages, such as, 150 days, 165 days and 180 days after the pollination, it was highest as 5% in the orchid whose seeds had been harvested 150 days after the pollination. In the result of examining the germination ratio of the seeds treated with NaOCl, the those treated with 2% of NaOCl showed the highest as 67% in the germination ratio.

A Review of Orchid Mycorrhizae in Korea

  • Lee, Sang-Sun
    • The Plant Pathology Journal
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    • v.18 no.4
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    • pp.169-178
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    • 2002
  • Orchids are evolutionally known to be the most advanced plants in the order Liliales, and comprise approximately 1,000 genera and 35,000 species world-wide. In Korea, more than 110 species of Orchidaceae have been reported to be cultivated or to be collected in the wild. Orchids aye mostly dependant on orchid mycorrhizae(OM) throughout or in part of their life cycle. The OM endomycorrhizae belonging to basidiomycetes or rarley ascomycetes are needed for orchid seed germination. Various fungi, including plant pathogenic, antagonistic and symbiotic fungi, were isolated from the roots of orchid native to Korea. The OM fungi collected from the roots of Cymbidium goeringii were three species of Rhizoctonia namely, R. repens (anamorph state of Tulsanella repens), R. endophytica (Ceratobasidium cornigerum), and an unidentified species (possibly an anamorph of T. calospora). These symbiotic fungi induced peloton in the cortical cells of orchid roots, and differed biologically and in 18s rDNA sequences from plant pathogenic Rhizoctonia species. Also, the mycorrhyzal fungi enhanced the orchid root absorption of nitrogen sources and minerals from the soil. The activity of mycorrhizal fungal hyphae in the roots caused prevention from pathogenic fungi. In nature, the peloton is observed in the cortical cells of Cymbidium goeriingii roots, indicating mycorrhizal colonization in the native orchid roots. On the other hand, pathogenic fungi such as Fusarium and/or Rhizoctonia species are mostly isolated from commercial orchid plants. These suggest that application of symbiotic mycorrhizal fungi should be needed for orchid cultivation in nurseries and at the time of transplanting.

Identification of the Orchid Mycorrhizal Fungi Isolated from the Roots of Korean Native Orchid

  • Lee, Sang-Sun;You, Jae-Hyung
    • Mycobiology
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    • v.28 no.1
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    • pp.17-26
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    • 2000
  • The orchid symbiotic fungi were isolated from the roots of Korean native orchid (Cymbidium goeringii) collected and Chinese orchid (C. sinense) obtained from greenhouses. They were identified as a species of Rhizoctonia, based on the sequences of 18r rDNA, the microscopic observations of mycelia, and the symbiotic relationships with commercial orchids. The isolate collected from Chinese orchids was revealed to be a species of Ceratobasidium endophytica, and to be different from the other isolates at the thickness of the mycelia stained in the root cells of Korean native orchids. The other isolates collected from the Korean native orchids were considered to be a species of Tulsanella repens (anamorphic: Epulorhiza repens) or its related one. The physiologic or microscopic variations were oftenly observed among them, but the tendency of grouping these in the 18s rDNA sequences were observed to be consistent with those of the localities collected. The further taxonomical segregating for Korean symbiotic fungi was not made because the information concerned were limited in this moment, but was recognized as based on the sequences of 18s DNA.

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Distribution and Vegetation Structure of Genus Cymbidium (Orchidaceae) in Jeju Island (제주도 내 난과(Orchidaceae) 보춘화속(Cymbidium)식물의 분포 및 자생지의 식생 구조)

  • Hyun, Hwa-Ja;Kim, Hae-Ran;Choi, Hyung Soon;Kim, Chan-Soo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.16 no.1
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    • pp.1-10
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    • 2014
  • This study was carried out to investigate the range of distribution and vegetation structure of habitats of genus Cymbidium which is distributed in Jeju Island, Korea. This genus is distributed in tropical and subtropical Asia and North Australia. In Korea, there are six species [i. e. C. goeringii (Rchb. f.) Rchb. f., C. kanran Makino, C. lancifolium Blume var. aspdistrifolium (Fukuy.) S. S. Ying., C. macrorhizum Lindl., C. nagifolium Masam. and C. ensifolium L.], which were also distributed in Jeju Island. The habitats of this genus ranged between 16 meter and 574 meter above sea level in Jeju Island. C. macrorhizum had the broadest altitudinal range (16-574 meter above sea level), whereas C. ensifolium, C. nagifolium and C. lancifolium var. aspidistrifolium had the narrow altitudinal range. According to the classification analysis by TWINSPAN, the plant communities were divided into five groups of Castanopsis sieboldii community, Castanopsis sieboldii-Pinus thunbergii community, Pinus thunbergii-Quercus acutissima community, Pinus thunbergii community and Quercus glauca- Aphananthe aspera community. Three species, C. kanran, C. nagifolium, and C. ensifolium inhabited Castanopsis sieboldii community and C. lancifolium var. aspdistrifolium inhabited Castanopsis sieboldii-Pinus thunbergii community. C. macrorhizum inhabited Pinus thunbergii community, Pinus thunbergii-Quercus acutissima community and Quercus glauca-Aphananthe aspera community. C. goeringii inhabitated Castanopsis sieboldii-Pinus thunbergii community and Pinus thunbergii-Quercus acutissima community. The populations of the genus Cymbidium have been extremely decreased due to illegal collection in Jeju Island. Especially, the distribution range of C. kanran, C. nagifolium, and C. ensifolium was narrow and limited to evergreen broad-leaved forests, therefore, conservation plans are needed for the protection of these species.

Development of Leaf Mutant Cultivars of Cymbidium goeringii by Ethyl-methane-sulfonate (EMS) Treatment. (Ethyl-methane-sulfonate(EMS) 처리에 의한 춘란 잎 돌연변이 품종의 개발)

  • Shin, Yun-Ho;Song, In-Ja;Kang, Eun-Jung;Bae, Tae-Woong;Sun, Hyeon-Jin;Kang, Si-Young;Lim, Pyung-Ok;Lee, Hyo-Yeon
    • Korean Journal of Plant Resources
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    • v.24 no.1
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    • pp.17-22
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    • 2011
  • This study was for developing leaf chlorophyll mutant cultivars of Cymbidium goeringii by ethyl-methanesulfonate(EMS) treatment. Chlorophyll mutant rhizomes were easily produced by 0.2% EMS treatment in this genus. Among the mutants, they became dark brown about 50% of the rhizomes. When the dark-brown rhizomes were cultured in a solidified MS medium, new rhizomes were formed from part of the old ones. Chlorophyll mutant rhizomes were obtained from subcultured meristem tissues of newly-formed rhizomes. The rhizomes were cut and subcultured for a year and then became mutant plants. As the results, they produced 4 kinds of leaf mutant cultivars; zigzag-striped, comb-striped, net-striped, and dwarf types, indicating that the EMS treatment in the rhizome could produce versatile leaf chlorophyll regulating mutants. These results suggest that our method is useful for developing leaf mutant cultivars of this planta which they are estimated as higher commercial values.

Vascular Plants and Specific Plants of Bulgapsan (불갑산 지역의 관속식물상과 특정식물)

  • 임동옥
    • Korean Journal of Environment and Ecology
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    • v.16 no.1
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    • pp.68-86
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    • 2002
  • The vascular plants in Bulgapsan, Younggwang-gun, Jeollanam-do, were consisted of 437 taxi; 104 families, 289 genus, 382 species, 51 varieties, 4 forma. From the floristic point of view, Bulgapsan is belongs to the South Province in Korea represented as Platycarya strobilacea, Cudrania tricuspidata, Mallotus japonicus, Lindera glauca, Euscaphis japonica, Meliosma myriantha, Euryu japonica, Halorrhagis micrantha, Hydeocotyle maritima, H. ramifloru, Chionanthus retusa, Trachelospermum asiaticum var. intermedium and Cymbidium goeringii, The Specific plants which is categorized to the Degree by the Ministry of Environment appeared to 25 taxa; Degree V was 2 species, Degree IV was 15 species, Degree III was 7 species and Degree II was 1 species. The mark evaluated by Degrees of five evaluation unit was 186.

The Vegetation of Seoimal-Lighthouse area in koje Island (거제 서이말등대 주변의 식생)

  • 김인택
    • Journal of Life Science
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    • v.12 no.6
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    • pp.649-653
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    • 2002
  • The vegetation of Seoimal-lighthouse area was investigated from August, 2001 to July, 2002. In order to analyze the vegetation of this area, synthesis table, actual vegetation map were prepared. The main community structures of this area represent evergreen broad-leaf community(about 48%), which are very good condition(nearly last sere) to preserve and 11communities(Camellia japonica community, Castanopsis cuspidnta var. thunbergii community, Neolitsea sericea community, Quercus serrata community, Quercus variabilis community, Carpinus corenna community, Srtrax japonica community, Pinus thunbergii community, Quercus acutissima community, Zelkova serrata community, Cinnamimum japonicum community) were classified by vegetation type. And Daphne kiusiana, Asirum maculatum, Cymbidium goeringii, Chloranthus glaber, Ligularia taguetii should be worthwhile species to conserve in this area.

Isolations of the Orchid mycorrhizal Fungi from the Roots of the Korean native Orchids and Inoculations of the Isolates to Four different Orchids (한국 자생란에 난 균근균의 분리와 유묘난에 접종)

  • 이상선;오창호;백기엽;이태수
    • Korean Journal Plant Pathology
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    • v.14 no.5
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    • pp.536-542
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    • 1998
  • Ten isolates of the orchid mycorrhizal fungi were isolated from the roots of Korean native orchid plants (Cymbidium goeringii) which inhabitate mainly in southern and western areas of Korea. The growth rates and color of the isolates in potato dextrose agar (PDA) were various. Microscopic observations of the hyphae isolated were identified as Rhizoctonia repens and R. endophytica var endophytica or their related species. R. repens was isolated from the roots of the Korean native orchids, but R. endophytica var endophyica was only isolated from the roots of the commercial orchids introduced from foreign countries. Also, the polymorephic patterns of genomic DNA extracted from selected isolates were compared with those of DNA extracted from the orchid mycorrhizal fungi isolated previously and similar band patterns were observed among those isolates. Five isolates of R. repens were selected and cultured at the oatmeal agar for investigating their symbiosis with orchid plants. The symbiotic specificity between orchid plants and isolated orchid mycorrhizal fungi was observe by growing orchids about six months in the greenhouse. The symbiotic responses of the commercial orchid plants with selected isolates were quite different form different isolates due to the genetic variations.

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