• 약학회지
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• 제43권3호
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• pp.378-384
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• 1999

Retinoic acid (RA) and dibutyryl cyclic AMP (dbcAMP) induce the differentiation of the multipotent embryonic carcinoma cell line, F9 cells, into parietal endoderm like cell. The F9 cells are highly proliferative doubling approximately 12 hourse. S Phase is predominant, lasting 10 hours and G2/M phase occupies most of the remaining cycle (2 hours) and G1 phase is nearly non-existent. In this study, we showed the effect of RA and dbcAMPon the cell cycle associated molecules (especially around G1 phase) during F9 cell differentiation. Differentiation of F9 cells was induced by the combined addition of RA ($10^{-7}M$) and dbcAMP (0.5mM), and cells were harvested daily up to 4 days. Flow cytometric analysis showed the prolongation of G1 phase around 30 hours after induction. Western blot analysis revealed that the amount of cyclin D1 and cdk2 were increased at day 4. However, histone H1 kinase activity of cdk2 was decreased. These data strongly suggest that RA and dbcAMP induce the growth arrest of F9 cells at G1 phase by decreasing the activity of cdk2, although they have increased the protein contents of cyclin D1 and cdk2. The reason for the discrepancy between the H1 kinase activity and protein contents are not clear yet.

• 한국항공우주학회지
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• 제36권7호
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• pp.695-702
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• 2008

액체 로켓 엔진의 연료 공급 시스템은 다양한 원인으로 인해 유동 불안정이 발생한다. 특히 연료 공급 시스템에서 발생하는 공동 현상은 공동의 생성과 소멸로 인해 후류 쪽의 압력 및 유량의 진동을 유발하게 된다. 액체 로켓은 주 추진제로 극저온 유체를 사용하게 되는데, 극저온 유체는 온도에 민감한 성질을 갖고 있기 때문에 공동 현상의 해석에 있어 온도 변화에 대한 효과를 반드시 고려해주어야 한다. 본 연구에서는 Shyy등이 제안한 “MUSHY IDM"모델을 이용하여 극저온 유체에서 발생하는 공동 현상을 모사하였다. 이를 바탕으로 오리피스에서 발생하는 공동 현상이 유동 불안정에 어떠한 영향을 미치는 지와 오리피스 형상 변화가 후류 유동의 불안정에 끼치는 영향을 연구하였다.

• 한국자동차공학회논문집
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• 제2권1호
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• pp.51-64
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• 1994

Analytical study on the thermodynamical cyclic behabiour and characteristics of HFC-l34a refrigerant for automotive air conditionser system for the replacement of existing CFC-12 has been carried out in this paper through development of system performance simulation program, expecially in the view point of system design considerations. The results indicate that HFC-l34a system will give a greater refrigerating capacity than CFC-12 if appropriate engineering measures such as proper codensers, flow controllers, etc., taken for certain operating conditions. The results, however, also show that the operating power for compression process increases over entire temperature range as a result of decreasing volumetric efficiency due to larger specific volume and increased discharging pressure. The present study results indicate that proper selection of condensing and evaporating temperature plus refrigerant control is very important performance factor to have better COP in the HFC-134a system design.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2010년 춘계학술대회논문집
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• pp.524-529
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• 2010

In the present study, a CFD program is developed for the Fluid-Structure Interaction(FSI) analysis. The non-staggered, non-orthogonal, and unstructured grid system was also used to handle the complicated geometries in the program. In order to validate the capabilities of the developed CFD program, various models are investigated by using unstructured and nonorthogonal meshes. The predicted results are a good agreement with analytic solution, experimental data and commercial software. And also PISO algorithm is applied for transient flow analysis. The cyclic boundary condition and baffle cell are developed in order to improve the effectiveness of the calculation for complex geometry.

• 동력기계공학회지
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• 제11권3호
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• pp.41-46
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• 2007

The microstructure and electrochemical analysis of welds of electric resistance welding(ERW) pipe were investigated. The direction of metal flow line in HAZ of ERW pipe shifted to the inner(or outer) surface of pipe by plastic deformation during welding. The lowest heat input welds of ERW pipe was showed crack by liquid penetrant testing. Accelerated corrosion test by constant current density of 20mA/$cm^{2}$ developed groove at the welds of ERW pipe and the measured grooving factors were about $1.2{\sim}1.5$. Corrosion potential of base metal obtained by cyclic polarization in artificial sea water(3.5wt.% NaCl solution) was 100mV higher than that of weld metal of ERW pipe.

• Journal of Advanced Marine Engineering and Technology
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• 제25권1호
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• pp.182-190
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• 2001

The aerodynamics of the Wells turbine has been studied using 3-d, unstructured mesh flow solver for the Reynolds-averaged Navier-Stokes equations. The basic feature of the Wells turbine is that even though the cyclic airflow produces oscillating axial forces on the airfoil blades, the tangential force on the rotor is always in the same direction. Geometry used to define 3-D numerical grid is based upon that of an experimental test rig. The 3-D Wells turbine model, consisting of approximate 220,000 cells is tested of four axial flow rates. In the calculations the angle of attack has been varied between 10˚ and 30˚ of blades, Representative results from each case are presented graphically andy analysed. It is concluded that this technique holds much promise for future development of Wells turbines.

• 대한기계학회논문집A
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• 제25권12호
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• pp.2056-2061
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• 2001

Non-proportional loading tests of Nylon 66 at room temperature exhibit path dependent behavior and plasticity-relaxation interactions. The uniaxial formulation of the viscoplasticity theory based on overstress (VBO), which has been used to reproduce the nonlinear strain rate sensitivity, relaxation, significant recovery and cyclic softening behaviors of Nylon 66, is extended to three-dimensions to predict the response in strain-controlled, comer-path tests. VBO consists of a flow law that is easily written for either the stress or the strain as the independent variable. The flow law depends on the overstress, the difference between the stress and the equilibrium stress that is a state variable in VBO. The evolution law of the equilibrium stress in turn contains two additional state variables, the kinematic stress and the isotropic stress. The simulations show that the constitutive model is competent at modeling the deformation behavior of Nylon 66 and other solid polymers.

• 오토저널
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• 제14권3호
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• pp.80-89
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• 1992

An experimental study of in-cylinder of flow and combustion characteristics in two gasoline engines of different intake ports which are denoted as original port and masked shroud head (MSH) ports is presented. The flows generated by the MSH and the original port are invest- igated by laser Doppler velocimeter(LDV) under steady flow and motoring (non-firing) condit -ions. Combustion characteristics with different swirl levels produced by two intake ports are analyzed by combustion pressure measurement and statistical calculation. The swirl inside the cylinder of the MSH port engine is found to be much higher than the original port, and the MSH has a large eddy motion of cylinder diameter size. Using ensemble average method to valuate engine turbulence under motoring condition, the MSH port engine is shown to have h -igher turbulence intensity than the original port, so that the effect of the MSH port on fast burn is shown. Also the cyclic variations of peak pressure and the reaching time in the MSH port are apparently reduced.

• 한국해양공학회지
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• 제7권1호
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• pp.114-123
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• 1993

In is well known that a cyclic Karman vortex street is thrown out periodically from cylinder whose axis is vertical to the bulk flow. When the cylinder is vibrating in the frequency close to that of Karman vortex street, the vortex shedding frequency is locked onto that of cylinder. While there are many experimental studies for this phenomenon compared with analytical studies by numerical calculation, are very limited. In this study, a new algorithm for moving boundary is proposed and a simulator is develoed, which can deal with this phenomenon with experimental studies.

• Steel and Composite Structures
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• 제27권3호
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• pp.337-353
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• 2018

In previous weak-axis moment connection tests, brittle fracture always initiated near the edge of the beam flange groove weld due to force flow towards the stiffer column flanges, which is the opposite pattern as strong-axis moment connections. As part of the China NSFC (51278061) study, this paper tested two full-scale novel weak-axis reduced beam section moment connections, including one exterior frame connection specimen SJ-1 under beam end monotonic loading and one interior frame joint specimen SJ-2 under column top cyclic loading. Test results showed that these two specimens were able to satisfy the demands of FEMA-267 (1995) or ANSI/AISC 341-10 (2010) without experiencing brittle fracture. A parametric analysis using the finite element software ABAQUS was carried out to better understand the cyclic performance of the novel weak-axis reduced beam section moment connections, and the influence of the distance between skin plate and reduced beam section, a, the length of the reduced beam section, b, and the cutting depth of the reduced beam section, c, on the cyclic performance was analyzed. It was found that increasing three parametric values reasonably is beneficial to forming beam plastic hinges, and increasing the parameter a is conducive to reducing stress concentration of beam flange groove welds while increasing the parameters b and c can only reduce the peak stress of beam flange groove welds. The rules recommended by FEMA350 (2000) are suitable for designing the proposed weak-axis RBS moment connection, and a proven calculation formulation is given to determine the thickness of skin plate, the key components in the proposed weak-axis connections. Based on the experimental and numerical results, a design procedure for the proposed weak-axis RBS moment connections was developed.