• Journal of KIISE:Software and Applications
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• v.29 no.3
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• pp.202-210
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• 2002

During the last few years, the extraction and reconstruction of the blood vessels in the medical image has been actively researched and the analysis for the retinal vessel structure has provided important information for diagnosis and remedy of the retinopathy patients. In this research, we propose the algorithm that tracks automatically the entire retinal vessel in retinal image acquired by the ICG(IndoCyanine Green) technology. This algorithm extracts contours and centers by estimating the local maxima and processing directions and detects bifurcations and junctions by comparing direction components of the local maxima from the gradient magnitude profile of each blood vessel. We present experimental results that the entire blood vessel is automatically reconstructed and is excellent in accuracy and connectivity after applying our algorithm to the ICG retinal images of patients.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2005.05a
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• pp.213-215
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• 2005

The metal complexes can be influenced not only by the central metal atoms and the substituent groups, but also by the native of the chelating atoms. For example, near-infrared absorbing chromophores were synthesized by the reaction of phenylenediamine derivatives with a solution of pottassium hydroxide followed by the addition of nickel(II) chloride. These dyes provide absorbing infrared light over 780-840 nm with an extinction coefficient of $2.5-6.0{\times}10^4$. By introduction of alkyl, alkoxyl, cyano, and other functional group into the parent dye, these dyes greatly improved the solubility in organic solvent. New near-infrared absorbing donor-acceptor chromophores have been investigated by varying the electron donating and accepting strength of the two halves of the molecule. The cyanine chromophores permit the simplest way of obtaining systems that absorb well into the near-infrared region of the spectrum. Cyanine dyes possess high extinction coefficients that initially increase with Increasing chain length. These chromophores could be useful in near-infrared optical materials.

• Korean Journal of Veterinary Research
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• v.58 no.4
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• pp.183-192
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• 2018

Although hyaluronic acid (HA) has been developed as a nanoparticle (NP; 320-400 nm) for a drug delivery system, the tissue targeting efficacy and the pharmacokinetics of HA-NPs are not yet fully understood. After a dose of 5 mg/kg of cyanine 5.5-labeled HA-NPs or HA-polymers was intravenously administrated into mice, the fluorescence was measured from 0.5 h to 28 days. The HA-NPs fluorescence was generally stronger than that of HA-polymers, which was maintained at a high level over 7 days in vivo, after which it gradually decreased. Upon ex vivo imaging, liver, spleen, kidney, lung, testis and sublingual gland fluorescences were much higher than that of other organs. The fluorescence of HA-NPs in the liver, spleen and kidney was highest at 30 min, where it was generally maintained until 4 h, while it drastically decreased at 1 day. However, the fluorescence in the liver and spleen increased sharply at 7 days relative to 3 days, then decreased drastically at 14 days. Conversely, the fluorescence of HA-polymers in the lymph node was higher than that of HA-NPs. The results presented herein may have important clinical implications regarding the safety of as self-assembled HA-NPs, which can be widely used in biomedical applications.

• Journal of KIISE:Software and Applications
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• v.27 no.10
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• pp.1054-1061
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• 2000

영상 정합은 물리적으로 유사한 영상 내의 영역들을 기하학적으로 일치시키는 처리이며 지형 정보, 영상검색, 원격탐사, 의료영상 등의 많은 영상처리 응용에서 사용된다. 영상 정합에 관한 연구는 주로 회전, 크기, 위치 등의 인자 추출에 소요되는 시간과 정확성에 중점을 두어 왔다. 본 연구에서는 영상의 특징 점들에 대한 일차 고유벡터의 방향 분포를 히스토그램으로 표현하고 이를 비교 분석함으로써 정합하는 방법을 제안한다. 일차 고유벡터를 이용함으로써 특징 묘사의 단순성을 제공하고. 히스토그램을 이용하여 정합 인자를 미리 추정함으로써 정합 인자 추출 시 목적함수의 연산에 소요되는 비용을 현저하게 줄였다. 본 연구의 결과를 평가하기 위해 제안한 방식을 일반 영상과 ICG(IndoCyanine Green)망막 영상에 적용한 결과를 보여주고 목적함수의 연산횟수와 시간 복잡도를 기존의 방법들과 비교하였다.

• Korean Journal of Veterinary Service
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• v.30 no.4
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• pp.489-496
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• 2007

An oligonucleotide microarray system was developed for the simultaneous detection of porcine epidemic diarrhea virus, transmissible gastroenteritis virus, porcine enteric calicivirus, porcine group A and C rotavirus. RNAs of the reference viruses and porcine diarrhea samples were extracted and amplified using one-step multiplex RT-PCR in the presence of cyanine 5-dCTP and hybridized on the microarray chip that spotted the virus-specific oligonucleotides. This system were approximately 10-to 100-fold higher in sensitivity than conventional RT-PCR, and the assay time was less than 3 hours. The relative sensitivity and specificity were 92% and 72.2%, respectively, based on 102 porcine diarrhea samples using RT-PCR as gold standard. These results suggested that the oligonucleotide microarray system in this study be probably more reliable and reproducible means for detecting porcine enteric viruses and that it could be of substantial use in routine diagnostic laboratories.

• Textile Coloration and Finishing
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• v.5 no.3
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• pp.206-215
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• 1993

In the prior study, wool gabardines were treated with alkaline proteases which were some kinds of enzyme to decompose protein, and their tensile strengths were determined, and the surface of the fibers were also observed using a scanning electron microscope. Enzylon ASN 30 and Alkalase 2.5L DX did not show much effect on the weight loss of wool, however, the weight loss of wool increased considerably with treating Esperase 8.0L. Pretreatment of wool with dichloloisocyanuric acid before protease-treatment increased the weight loss of wool to a great extent. In this study, the enzyme treated wools dyeing behaviors with acid dye, Milling Cyanine 5R, were mainly investigated. The protease-treatment remarkably increased not only the rate of dyeing but also the saturation dye uptake. From these results, it seemed likely that the structural relaxation of adhesive filler of interscale or intercellular cement facilitated the dye penetration into the fibers, at the same time, the change in the inner structure of the wool fibers by the protease made the fixation of the dyes more efficient.

• Textile Coloration and Finishing
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• v.1 no.1
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• pp.47-53
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• 1989

The effect of organic solvent in the dyeing of silk fiber by acid dye was investigated. Acetophenone and benzyl alcohol were shown to be the most effective for the rate of dyeing of silk fiber by Milling Cyanine 5R (C.I. Acid Blue 113), a milling type acid dye, but, with benzyl alcohol, the equilibrium dye uptake was much lower than that in the absence of it. In the presence of solvent, maximum dye uptake shifted to lower temperature than 6$0^{\circ}C$, while without solvent, it was shown at about $60^\circ{C}$. When dyed by Orange II (C.I. acid Orange 7) under same condition equilibrium dye uptake of silk fiber was lower than that for milling type acid dye, and in the presence of benzyl alcohol, still much lower uptake resulted. All these fact reveals that organic solvents in the solvent-assisted dyeing of silk fiber broaden micelle spacings too much, resulting in increased rate of dyeing, and decreased equilibrium dye uptake, contrary to wool.

• Textile Coloration and Finishing
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• v.21 no.5
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• pp.35-40
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• 2009

Computational calculations of molecular orbital and electrochemical redox/oxidation potentials are of very importance to determine the compound properties. The energy levels of molecular orbital were calculated by the density function theory (DFT) with exchange correction functional of local density approximation (LSA) based on the Perdew-Wang (PWC) setting and cyclic voltammetry.

• Bulletin of the Korean Chemical Society
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• v.26 no.3
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• pp.428-432
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• 2005

Squarylium or squaraine dyes are derived from 1,2-dihydroxycyclobuten-3,4-dione, otherwise known as squaric acids. They are two principal types: the 1,2-bisdonorsubstituted derivatives, and the 1,3-bisdonorsubstituted derivatives. The former are essentially merocyanines and have no distinctive properties, whereas the latter represent a unique type of chromophore, which is neither a merocyanines nor cyanine and has exceptional light absorption characteristics. They also have many functional applications based on their special properties. Thus it was the objective of this research project to synthesize a range of 1,3-squarylium dyes of widely differing structural types, and to investigate their light absorption and fluorescence properties in general, and the color change properties of appropriate examples in particular. Also in this study, the various pHinduced colour change processes were examined.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2008.10a
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• pp.133-134
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• 2008

A novel near infrared(NIR) absorbing dyes were synthesized by using bis-aldehyde formyl aromatic compounds and heteroaryl derivatives with the reactive methylene group. These dyes provided a range of the NIR wavelength region about 720 nm value. Also, the light absorbing properties of these dyes were investigated in our experiment results. In generally, these NIR colorants may be potential used for optical recording media, DNA sequencing probe and laser beam printings.