• 한국균학회지
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• 제14권2호
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• pp.179-183
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• 1986

1985년(年)에 수입(輸入)한 종자(種子)에서 검출(檢出)된 진균(眞菌) 24종(種) 중(中) 일본산(日本産) 시금치에서 Verticillium dahliae 잔디에서 Exserohilum halodes 중공산(中共産) 수수에서 Chaetomium globosum과 Chaetomium elatum, Curvularia intermedia, 대만산(臺灣産) 야자에서 Graphium putredinis, Phaeoisaria clematides, 그리고 대만산(臺灣産)과 태국산(産) 야자와 관음죽에서 Macrophomina sp. 등(等)이 조사(調査)되어 그 결과(結果)를 보고(報告)하는 바이다.

• The Plant Pathology Journal
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• 제33권6호
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• pp.597-601
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• 2017

The clpks18 gene was first cloned and identified in Curvularia lunata. It contains 6571 base pairs (bp) and an 6276 bp open reading frame encoding 2091 amino acids. The ClPKS18 deletion mutant displayed an albino phenotype, and almost lost the ability to product 5-(hydroxymethyl) furan-2-carboxylate (M5HF2C) toxin, implying that clpks18 gene in C. lunata is not only involved in 1,8-dihydroxynaphthalene melanin synthesis, but also relatively associated with M5HF2C toxin biosynthesis of the pathogen. The pathogenicity assays revealed that ${\Delta}ClPKS18$ was impaired in colonizing the maize leaves, which corresponds to the finding that ClPKS18 controls the production of melanin and M5HF2C in C. lunata. Results indicate that ClPKS18 plays a vital role in regulating pathogenicity of in C. lunata.

• Mycobiology
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• 제30권4호
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• pp.225-227
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• 2002

The inhibitory activity of ent-norsecurinine alkaloid was evaluated against spore germination of some plant pathogenic fungi(Curvularia maculans, Curvularia species, C. palliscens, Colletotrichum gloeosporioides, Colletotrichum species, Afternaria solani, A. brassicae, Fusarium udum, Helminthosporium echinoclova and H. penniseti). It inhibited spore germination of all the test fungi. C. maculans, C. species, and C. palliscens were the most sensitive as complete inhibition of spore germination was observed at 1000 ppm. A. solani was not inhibited by this chemical.

• 한국균학회지
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• 제51권3호
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• pp.245-249
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• 2023

Prickly lettuce (Lactuca serriola), an invasive annual plant, poses a significant threat to the agricultural systems of many countries, including Korea. In 2020, leaf blight symptoms were observed in the prickly lettuce populations of various farms across Korea. Detailed morphological and molecular sequence analyses revealed that the disease was caused by the fungus, Curvularia intermedia. A pathogenicity test confirmed that the fungus can cause the same symptoms in healthy prickly lettuce, thereby fulfilling Koch's postulates. To the best of our knowledge, this is the first report of C. intermedia causing leaf blight on L. serriola in Korea, suggesting its potential as a biocontrol agent for this weed. However, further investigations are necessary to determine its ecological impact to prevent any non-target effects.

• The Plant Pathology Journal
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• 제31권2호
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• pp.108-114
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• 2015

Curvularia lunata is an important maize foliar fungal pathogen that distributes widely in maize growing area in China, and several key pathogenic factors have been isolated. An yeast two-hybrid (Y2H) library is a very useful platform to further unravel novel pathogenic factors in C. lunata. To construct a high-quality full length-expression cDNA library from the C. lunata for application to pathogenesis-related protein-protein interaction screening, total RNA was extracted. The SMART (Switching Mechanism At 5' end of the RNA Transcript) technique was used for cDNA synthesis. Double-stranded cDNA was ligated into the pGADT7-Rec vector with Herring Testes Carrier DNA using homologous recombination method. The ligation mixture was transformed into competent yeast AH109 cells to construct the primary cDNA library. Eventually, a high qualitative library was successfully established according to an evaluation on quality. The transformation efficiency was about $6.39{\times}10^5$ transformants/$3{\mu}g$ pGADT7-Rec. The titer of the primary cDNA library was $2.5{\times}10^8cfu/mL$. The numbers for the cDNA library was $2.46{\times}10^5$. Randomly picked clones show that the recombination rate was 88.24%. Gel electrophoresis results indicated that the fragments ranged from 0.4 kb to 3.0 kb. Melanin synthesis protein Brn1 (1,3,8-hydroxynaphthalene reductase) was used as a "bait" to test the sufficiency of the Y2H library. As a result, a cDNA clone encoding VelB protein that was known to be involved in the regulation of diverse cellular processes, including control of secondary metabolism containing melanin and toxin production in many filamentous fungi was identified. Further study on the exact role of the VelB gene is underway.

• Mycobiology
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• 제39권3호
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• pp.206-218
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• 2011

The seed-borne mycoflora of sorghum and foxtail millet collected from different growing areas in South Korea were isolated and taxonomically identified using dry inspection, standard blotter and the agar plate method. We investigated the in vitro and in vitro germination rates of disinfected and non-disinfected seeds of sorghum and foxtail millet using sterilized and unsterilized soil. The percent recovery of seed-borne mycoflora from the seed components of sorghum and foxtail millet seeds was determined and an infection experiment using the dominant species was evaluated for seedling emergence and mortality. A higher number of seed-borne fungi was observed in sorghum compared to that of foxtail millet. Eighteen fungal genera with 34 fungal species were identified from the seeds of sorghum and 13 genera with 22 species were identified from the seeds of foxtail millet. Five dominant species such as Alternaria alternata, Aspergillus flavus, Curvularia lunata, Fusarium moniliforme and Phoma sp. were recorded as seed-borne mycoflora in sorghum and 4 dominant species (Alternaria alternata, Aspergillus flavus, Curvularia lunata, Fusarium moniliforme) were observed in foxtail millet. The in vitro and in vitro germination rates were higher using disinfected seeds and sterilized soil. More seed-borne fungi were recovered from the pericarp compared to the endosperm and seed embryo. The percent recovery of seed-borne fungi ranged from 2.22% to 60.0%, and Alternaria alternata, Curvularia lunata and 4 species of Fusarium were isolated from the endosperm and embryo of sorghum and foxtail millet. Inoculation of the dominant seed-borne fungi showed considerable mortality of seedlings. All the transmitted seed-borne fungi might well be a primary source of infection of sorghum and foxtail millet crops.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 1999년도 정기총회 및 춘계 학술발표회
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• pp.15.1-15
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• 1999

• Mycobiology
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• 제33권2호
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• pp.97-103
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• 2005

Crude extracts and active principles from medicinal plants have shown potential role in controlling plant diseases in glasshouses as well as in fields as one of the safest and ecofriendly methods. The effect of nor-securinine (an alkaloid) isolated from Phyllanthus amarus has been seen against spore germination of some fungi (Alternaria brassicae, A. solani, Curvularia pennisetti, Curvularia sp., Erysiphe pisi, Helminthosporium frumentacei) as well as pea powdery mildew (Erysiphe pisi) under glasshouse conditions. The sensitivity of fungi to nor-securinine varied considerably. Nor-securinine was effective against most of the fungi. H. frumentacei was more sensitive even at the lowest concentration ($1,000\;{\mu}g/ml$). Likewise conidia of E. pisi were also inhibited in partially or completely appressorium formation. Pre-inoculation treatment showed greater efficacy than post-inoculation in inhibiting powdery mildew development on pea plants in a glasshouse. Maximum inhibition occurred at $2000\;{\mu}g/ml$.

• Mycobiology
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• 제32권4호
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• pp.160-163
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• 2004

Medicinal plants play important roles in controlling plant diseases as one of the safest and ecofriendly methods. These plants have been used in the form of crude extracts as well as active principles in vitro and under field conditions to control plant diseases. Among the active principles, alkaloids have shown significant antifungal activity. We have investigated the effect of two alkaloids viz., (-)-corydahnine and (-)-isocorypahnine isolated from Corydalis chaerophylla, against spore germination of some plant pathogenic and saprophytic fungal spores. Significant inhibition of spore germination at 100 ${\mu}g/ml$ was seen against Curvularia penniseti, Curvularia sp. and Colletotrichum gloeosporioides by (-)-corydahnine but (-)-isocorypalmine was also effective against fungi included in the experiment.

• 농업과학연구
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• 제12권2호
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• pp.199-205
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• 1985

1. 벼 종자(種子)의 변색정도(變色程度)에 따른 균류(菌類)의 검출(檢出)을 조사(調査)한 결과(結果) 변색(變色)이 심한 종자(種子)에서 Alternaria tenuis, Cladosporium cladosporioides, Curvularia lunata, Fusarium semitectum, Gerlachia oryzae, Phoma sp. Trichoconiella padwickii 등이 높은 비율(比率)로 검출(檢出)되었고 특히 반점종자(斑點種子)에서는 Helminthosporium 균(菌)이 높은 비율(比率)로 검출(檢出)되었다. 2. 벼 종자상(種子上)에서 진균(眞菌)의 검출(檢出)에 미치는 광선(光線)의 영향(影響)을 조사(調査)한 결과(結果) Alternaria tenuis, Helminthosporium oryaze, Trichoconiella padwickii, Fusarium moniliforme, Fusarium semitectum 등(等)은 암흑구(暗黑區)에서 보다 광선조사구(光線照射區)에서 검출율(檢出率)이 높았고 Phoma sp. Curvularia lunata, Epicoccum purpurascens, Verticillium sp.는 광선조사구(光線照射區)나 암흑구(暗黑區)나 거의 차이가 없었으며 Gerlachia oryzae는 암흑구(暗黑區)에서 오히려 검출율(檢出率)이 높았다. 3. 벼 종자상(種子上)의 배양온도(培養溫度)가 균류(菌類)의 검출(檢出)에 미치는 영향(影響)을 조사(調査)하였던 바 Alternaria tenuis, Cladosporium cladosporioides는 저온구$(15^{\circ}C)$에서 검출율(檢出率)이 높았고 Phoma sp.는 고온구$(30^{\circ}C)$에서 검출율(檢出率)이 높았다. 4. 검출(檢出)된 각(各) 균류(菌類)가 종자(種子) 변색(變色)및 수량(收量)에 미치는 영향(影響)을 조사(調査)하였던 바 공시(供試)한 7게(個)의 균류(菌類) 모두가 종자(種子) 변색(變色)에 관여(關與)하여 인공접종(人工接種)한 이삭에서의 천립중(千粒重)은 대조구의 천립중(千粒重)에 비해 모두 낮았고 특히 공시(供試)한 7개(個)의 균(菌)을 혼합접종(混合接種)한 이삭의 천립중(千粒重)과 등숙비율(登熟比率)은 대조구보다 저하(低下)됨을 알 수 있었다.