• Title/Summary/Keyword: Cultures

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Ideal Maternal Behaviors as Viewed by Professionals in Three Different Cultures : Korea, the United States, and France (한·미·불 전문가의 시각에서 본 영아기 이상적인 모성행동)

  • Koo, Meehyahg;Lee, Yanghee
    • Korean Journal of Child Studies
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    • v.23 no.2
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    • pp.89-105
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    • 2002
  • This study investigated characteristics and types of ideal maternal behaviors in three different cultures : Korea, the United States, and France. The 18 experts from each of the 3 cultures who participated in this study had knowledge and experience related to infant development and parenting. Based on the principles of Q-methodology, the experts sorted 40 Q-items representing maternal behavior related to infancy. After rating each item on a scale from "least desirable" to "most desirable" maternal behaviors, the experts described their reasons for the classification. Results identified some clear similarities and differences among cultures. pooled responses yielded 3 types of ideal maternal behaviors: perfective, child centered, and realistic perspective.

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Production Enhancement of Menthol in Suspension Cultures of Peppermint Using Cyclodextrin (Peppermint 세포 현탁배양에서 Cyclodextrin을 이용한 Menthol의 생산성 증대)

  • 조규헌;임철호;박세춘;신명근
    • KSBB Journal
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    • v.13 no.1
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    • pp.26-30
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    • 1998
  • The suspension cultures of Mentha piperita produce menthol which has very low solubility in water due to its hydrophobicity. This can be considered as a factor for its low production in the suspension suspension cultures. Cyclodextrin has the hydrophobic cavity inside the molecule in which menthol can be captured and allow to form a stable complex. The suspension culture of Mentha piperita showed 70% higher production enhancement in the medium containing 1.5%(w/v) $\beta$-cyclodextrin than the control. $\beta$-cyclodextrin had no adverse effect on the cell growth and showed the best result among $\alpha$-, $\beta$- and $\gamma$-cyclodextrins tested in terms of menthol production. We demonstrated that $\beta$-cyclodextrin can be used to enhance the production of menthol in the suspension cultures by capturing hydrophobic menthol into the cavity of cyclodextrin molecules.

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Isoprenoid Quinone Profiles of the Leclercia adecarboxylata KCTC $l036^T$

  • Shin, Yong Kook;Jung Sook Lee;Chang Ouk Chun;Hong Joong Kim;Yong Ha Park
    • Journal of Microbiology and Biotechnology
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    • v.6 no.1
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    • pp.68-69
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    • 1996
  • The isoprenoid quinone composition of Leclercia adecarboxylata KCTC $1036^T$ was determined by using high-performance liquid chromatography. L. adecarboxylata KCTC $1036^T$ are characterized by their production of both ubiquinone-7, ubiquinone-8 and menaquinone-8 as major quinones. It is clear that the analysis of isoprenoid quinone profiles provides a new criterion of great promise for identifying Leclercia strains.

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Protective Effects of the Water Extract of Protaetia brevitarsis Larva Against Carbon Tetrachloride-Induced Toxicity in the Primary Cultures of Adult Rat Hepatocytes (랫드 일차 배양 간세포에서 사염화탄소의 독성에 대한 지잠 물추출물의 보호효과)

  • Yun, Soo-Hong;Kim, Duk-Hyun;Hyun, Sun-Hee;Lee, Sang-Kyu;Jeon, Tae-Won;Jeong, Tae-Cheon
    • YAKHAK HOEJI
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    • v.50 no.4
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    • pp.287-292
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    • 2006
  • Protective effects of the water extract of Protaetia brevitarsis larva against $CCl_4-induced$ toxicity were investigated in primary cultures of adult rat hepatocytes. The extract used in these studies contained several minerals, fatty acids and amino acids. Treatment of hepatocyte cultures with the extract provided a significant protection from the increased LDH activity induced by $CCl_4$. The results demonstrated that the extract may have the protective effect against $CCl_4-induced$ toxicity in hepatocyte cultures.

The Effect of Starter Culture on Viscosity of Stirred Yogurt (호상 요구르트의 점도에 미치는 균주의 영향)

  • Jeong, Tae-Hui;Kim, Nam-Cheol;Park, Heung-Sik;Gwak, Hae-Su
    • Journal of Dairy Science and Biotechnology
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    • v.24 no.1
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    • pp.65-73
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    • 2006
  • This study was to review recent reports in effects of various starter cultures on the viscosity in stirred yogurt. The rheological properties of yogurt have received considerable attention in the literature. Most yogurts are typically made by mixed cultures of Lactobacillus bulgaricus and Streptococcus thermophilus. The viscosity of yogurt made by mixed cultures was much higher than that of yogurt by single cultures. Since texture of stirred yogurt is the result of both acid aggregation of casein micelles and production of exopoly-saccharides, it is suggested that yogurt be made by the exopolysaccharide-producing cultures in order to increase viscosity, Both types of exopolysaccharides are capsule and loose slime(ropy). But it is desirable to use encapsulated nonropy strains. And Bifidobacteria affects adversely to the viscosity of yogurt. Therefore, starter cultures which have an effect on yogurt viscosity have been widely demonstrated. This review is the search for the development of viscosity in stirred yogurt.

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Optimization of Aconitine Production in Suspension Cell Cultures of Aconitum napellus L.

  • Hwang, Sung-Jin;Kim, Yun-Hee;Pyo, Byoung-Sik
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.5
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    • pp.366-371
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    • 2004
  • Aconitine alkaloids produced from cell suspension cultures of Aconitum napellus for the first time. The effects of various culture conditions on cell biomass and aconitine accumulation in cell suspension cultures were investigated. Suspension cell cultures of A. napellus were established by transferring callus tissues from leaf explants onto liquid MS medium supplemented with $1\;mg/l$ NAA and $0.1\;mg/l$ kinetin. Among the culture media tested, MS medium had a pronounced effect on cell growth and aconitine accumulation. The maximum dry cell weight was obtained at inoculum size of 3 g (FCW) per flask and in MS medium supplemented with 5% sucrose after 8 weeks. The addition of salicylic acid (SA) and yeast extract (YE) in the MS medium enhanced aconitine accumulation. Using a proper combination of culture condition and supplements, aconitine content could reach 0.043% (dry weight basis), that was $2.5{\sim}3$ fold higher that detected in control cultures.

Reinterpretation of Learning Environment Instruments from Cultural Perspectives - Exploring the Applicability for Understanding Science Classroom Cultures - (문화적 관점에서 학습환경 검사 도구 재해석하기 - 과학 교실문화 이해를 위한 활용가능성 탐색 -)

  • Chang, Jina;Na, Jiyeon;Song, Jinwoong
    • Journal of Korean Elementary Science Education
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    • v.34 no.2
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    • pp.238-251
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    • 2015
  • This study, based on literature review and theoretical discussion, reinterprets the learning environment instruments from cultural perspectives and suggests the applicability of learning environment instruments for understanding science classroom cultures. To do this, the existing learning environment instruments are first investigated and compared in terms of their features and utilizations appeared in previous studies. The learning environment instruments are then reinterpreted in the light of culture. Finally, we suggest the possibilities to use the learning environment instruments to understand science classroom cultures. The results of this study can be summarized as follows. First, the learning environment instruments, by interpreting them culturally, could be interpreted in cultural ways and used as the alternative ways to explore science classroom cultures. Second, the learning environment instruments, such as WIHIC and CLEQ, could be interpreted both along the dimension of phenomena in classrooms and the dimension of students' psychology in order to investigate science classroom cultures. Third, the instrument items could be interpreted culturally in different ways according to the description types of instrument items. Thus, when learning environment instruments are used in culture research, the description types should be sufficiently taken into account. Based on the results of this study, educational implications are discussed in terms of exploring classroom cultures and of culture research.

Propagation and Attenuation of Japanese Encephalitis Virus in Tissue Culture Cells (조직배양세포에서의 일본뇌염virus 증식에 관한 연구)

  • Lee, Ho-Wang;Moon, Seok-Bae
    • The Journal of the Korean Society for Microbiology
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    • v.16 no.1
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    • pp.83-89
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    • 1981
  • Japanese encephalitis has been prevalent for long time in the Far East and many patients have been reported in both South East and Mid-West Asia recently. Recently, vaccine was used in prevention of this viral disease of man which was derived from formalin inactivated virus inoculated into mouse brain, but live attenuated active vaccine for human is not developed yet. Author inoculated Japanese encephalitis virus into several cell culture strains for development of live attenuated encephalitis virus strain and the results were as follows: 1. Japanese encephalitis virus was inactivated rapidly in cell free medium at $36^{\circ}C$ and totally inactivated by 72 hours. 2. In growth curve of Japanese encephalitis virus in HeLa cell cultures, maximal multiplication of the virus was occured at 4th day and virus multiplication was continued for at least 12 days. 3. After succeeding passage of the virus in HeLa cell cultures and human esophagus epithelial cell cultures, infectivity of virus for mice was disappeared from 2nd passage in HeLa cell cultures and 3rd passage in esophagus epithelial cell cultures. 4. In inoculation to monkey kidney epithelial cells and chick embryo cell cultures, infectivity of the virus for mice was continued after 10th passages.

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Effects of Autochthonous Yeast Cultures on Some Quality Characteristics of Traditional Turkish Fermented Sausage "Sucuk"

  • Ozturk, Ismet;Sagdic, Osman;Yetim, Hasan
    • Food Science of Animal Resources
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    • v.41 no.2
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    • pp.196-213
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    • 2021
  • The objective of this study was to determine the effects of yeast cultures (Candida zeylanoides and Debaryomyces hansenii) isolated from traditionally dry fermented Turkish sucuks, on some physicochemical and microbiological properties of the product. Eight different batches of the sucuks were produced by the inoculation of yeast and lactic acid bacteria (LAB) cultures (Lactobacillus curvatus, Lactobacillus plantarum and Lactobacillus sakei) in different combinations. The sucuks were ripened for 12 days and analyzed at 1st, 6th, and 12th days of ripening. Percent moisture content, pH, water activity (aw) and residual nitrite values of the sucuk inoculated with the yeast cultures were higher at the end of the ripening. The use of yeast cultures decreased hardness, gumminess, and chewiness values of the sucuk while increased adhesiveness values. Major volatile groups were aldehydes, terpenes, and sulphur compounds in the sucuk samples. The most noticeable results were for sensory properties of the sucuk that were positively improved by the yeast cultures.