• Title/Summary/Keyword: Cultures

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Draft genome sequence of Olsenella sp. KGMB 04489 isolated from healthy Korean human feces (건강한 한국인 분변으로부터 분리된 Olsenella sp. KGMB 04489 균주의 유전체 염기서열 초안)

  • Han, Kook-Il;Kang, Se Won;Kim, Ji-Sun;Lee, Keun Chul;Eom, Mi Kyung;Suh, Min Kuk;Park, Seung-Hwan;Lee, Ju Huck;Park, Jam-Eon;Oh, Byeong Seob;Yu, Seung Yeob;Choi, Seung-Hyeon;Lee, Dong Ho;Yoon, Hyuk;Kim, Byung-Yong;Yang, Seung-Jo;Lee, Jung-Sook
    • Korean Journal of Microbiology
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    • v.54 no.4
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    • pp.456-459
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    • 2018
  • The genus of Olsenella has been isolated from vertebrate animal mouth, rumen, and feces. Olsenella sp. KGMB 04489 was isolated from fecal samples obtained from a healthy Korean. The whole-genome sequence of Olsenella sp. KGMB 04489 was analyzed using the PacBio Sequel platform. The genome comprises a 2,108,034 bp chromosome with a G + C content of 65.50%, 1,838 total genes, 13 rRNA genes, and 52 tRNA genes. Also, we found that strain KGMB 04489 had some genes for hydrolysis enzymes, and antibiotic biosynthesis and resistance in its genome based on the result of genome analysis.

A Report on the Shigella Cultures Isolated in Korea(1975) (1975년도(年度) 한국(韓國)에서 분리(分離)된 이질균(痢疾菌)에 관(關)한 보고(報告))

  • Kim, Soon-Hit;Chun, Nam-Ho;Ryu, Young-Hat
    • The Journal of the Korean Society for Microbiology
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    • v.11 no.1
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    • pp.23-26
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    • 1976
  • The authors identified sixteen Shigella cultures, which were three cultures of subgroup A, ten cultures of subgroup B, three cultures of subgroup D and none of cultures belnoging to the subgroup C was detected as it was in previous year.

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Amylase Production by Continuous Cultures of Aspergillus oryzae and its Mutants (Aspergllus oryza와 그 변이주의 연적배양에 의한 amylase 생성에 관한 연구)

  • Han, Hong-Eui
    • Korean Journal of Microbiology
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    • v.15 no.2
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    • pp.63-76
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    • 1977
  • Irradiation with high doses of gamma rays induced the reduction of mycelial weight and anaylase activity, and increased relative amylase activity in surface cultures. Biphase in growth curves was shown in aeration-agitation cultures but the behavior of the first phase of growth could be eliminated by replacing the amylasehydrolysed starch substrates, so that enzyme production was shortened ca. 40 hours and relative amylase activity was increased about 3 times higher before onset of autolysis. In the effect of gibberellin on amylase production, the positive stimulation was appeared to only surface culturs of the liquid medium and the negative effect to shake-cultures in a mutant. Trials of various continuous culture were resulted not only the approalch to the value of amylase activity in surface cultures of liquid medium, but also higher productivity than in batch cultures. The culture-degeneration was observed in two-stage continuous culture, but did not appear in continuous elevation culture.

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Effectiveness of Flashing Light for Increasing Photosynthetic Efficiency of Microalgal Cultures over a Critical Cell Density

  • Park, Kyong-Hee;Lee, Choul-Gyun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.3
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    • pp.189-193
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    • 2001
  • Critical cell density (CCD), the maximum cell concentration without mutual shading in algal cultures, can be used as a new operating parameter for high-density algal cultures and for the application of the flashing light effect on illuminated algal cultures. CCD is a function of average cell volume and light illumination area. The CCD is thus proposed as an index of estimation of mutual shading in algal cultures. Where cell densities are below the CCD, all the cells in photobioreactors can undergo photosysnthesis at their maximum rate. At cell densities over CCD, mutual shading will occur and some cells in the illumination chamber cannot grow photoautotrophically. When the cell concentration is higher than the CCD, specific oxygen production rates under flashing light were higher than those under continuous light. The CCD was found to be a useful engineering parameter for the application of flashing light, particularly in high-density algal cultures.

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Microbiological, PH and Sensory Evaluations of Refrigerated Pork Loins Treated with Lactococcus lactis ATCC l1454 (Lactococcus lactis ATCC 11454로 처리한 냉장 돼지고기 등심의 미생물, pH 및 관능평가)

  • 김창렬;김광현;이재일
    • The Korean Journal of Food And Nutrition
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    • v.14 no.2
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    • pp.126-131
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    • 2001
  • Microbiological, pH and sensory evaluations of pork loins treated with lactic acid cultures (Lactococcus lactis subsp. lactis ATCC 11454 grown in 10% milk solids) during storage at 4$^{\circ}C$ were assessed. Pork loins were immersed in solutions containing 3.96 log units, 4.10 log units, or 4.23 log units of individual lactic acid cultures for 2 min. Pork loins treated with lactic acid cultures had a inhibitory effect (P<0.05) on the growth of aerobic palate counts during storage of 3 days at 4$^{\circ}C$. Pork loins treated with lactic acid cultures during storage of 3 days at 4$^{\circ}C$ significantly (P<0.05) decreased pH values compared to those of controls. Sensory evaluations for odor and appearance of pork loins treated with lactic acid cultures during storage of 9 days at 4$^{\circ}C$ were a "liked less" to "typical" category compared to those of controls.

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Hybridization of Ethnic-Cultural Elements Shown in Domestic Fashion Magazines (국내 패션잡지에 나타난 민족적 요소의 하이브리드 경향)

  • Kim, Sun-Young
    • Journal of the Korean Society of Costume
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    • v.61 no.8
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    • pp.45-56
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    • 2011
  • This study is about the features of hybridization of ethnic-cultural elements in Korean fashion magazines. Its purpose was to embody more creative and newer images in fashion by promoting better mutual understanding of foreign cultures and addressing the issues of fashion design from multicultural perspectives. In doing so, the present study conducted a literature review and analyzed a total of 130 photographic images with any hybrid ethic element from two Korean fashion magazines, Vogue Korea and Harper's Bazaar Korea, issued between 2005 and 2009. The analysis revealed that there were 66 images(50.8%) with a mixture of two different cultures and 64 images(49.2%) with a mixture of three or more different cultures. As seen from the results, the two categories had a similar number of cases. In regional terms, the use of two different cultural elements included a mixture of Asian and Western cultures in 32 images(24.6%), a mixture of Western and African, Middle Eastern or Latin American cultures in 23(17.7%), and a mixture of Western and Russian or European folk cultures in 11(8.5%). In the use of three or more different cultural/national elements, the present study found a mixture of Asian, African, Middle Eastern, Latin American and Western costume items in 20 photo images(15.4%), a mixture of African, Middle Eastern and Latin American elements plus Western costume items in 19 images( 14.6%), a mixture of all regional cultures in 13 photos(10%), and a mixture of Asian ethic cultures plus Western costume items in 12 cases(9.2%). The results of this analysis indicated that the hybridization of ethnic-culture elements in Korean fashion magazines consisted of diverse clothing and accessories from various ethnic groups. The expression of these multi-cultural hybrid images that consists background images and models from different cultures well-portrayed the multi-cultural elements based on total coordination and broke the stereotypical aspects of styling.

Classification of Isolates Originating from Kimchi Using Carbon-source Utilization Patterns

  • LEE, JUNG-SOOK;CHANG OUK CHUN;MIN-CHUL JUNG;WOO-SIK KIM;HONG-JOONG KIM;MARTIN HECTOR;SAM-BONG KIM;CHAN-SUN PARK
    • Journal of Microbiology and Biotechnology
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    • v.7 no.1
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    • pp.68-74
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    • 1997
  • One hundred and eighty two lactic acid bacteria, isolated mainly from kimchi, including reference strains were examined for their ability to utilize 95 carbon sources. The test strains were assigned to 5 major, 1 minor and 12 single-membered clusters based on the $S_{SM}$, UPGMA algorithm (at similarity of $80{\%}$). These aggregate clusters were equivalent to the genus Leuconostoc (aggregate cluster M and N), the genus Lactobacillus (aggregate cluster Q and R), and the genera Lactobacillus and Leuconostoc (aggregate cluster O and P) according to the database of the Biolog system. This study demonstrates that rapid identification and classification of isolates originating from kimchi can be achieved on the basis of such carbon source utilization tests.

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Attraction of the Invasive Hornet, Vespa velutina nigrithorax, by using Bacillus sp. BV-1 Cultures

  • Lim, Da Jung;Lee, Jeong Eun;Lee, Jin Sil;Kim, Iksoo;Kim, In Seon
    • Korean Journal of Environmental Agriculture
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    • v.38 no.2
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    • pp.104-109
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    • 2019
  • BACKGROUND: The invasive hornet Vespa velutina nigrithorax has becomes a public concern in rural and urban South Korea. The technologies are necessary to develop a way to counter V. velutina. In an effort to develop a way to counter V. velutina, we found that a bacillus strain, named Bacillus sp. BV-1, produces volatile compounds that attract V. velutina. METHODS AND RESULTS: Field trials of V. velutina attraction were performed using plates and traps containing BV-1 cultures grown on sugar medium. When the sugar medium and sugar-grown BV-1 cultures in the plates were placed close together, V. velutina visited preferably the plates with the BV-1 cultures. Significantly more V. velutina were caught in the traps containing BV-1 cultures than in those containing only sugar medium. Headspace solid-phase microextraction coupled with GC/MS analysis of BV-1 cultures detected 2-methyl-1-propanol, 3-methyl-1-butanol, 3-methylbutanoic acid, ethyl hexanoate, 2-pheylethanol, ethyl octanoate, and ethyl decanoate as the major volatiles. CONCLUSION: BV-1 cultures were suggested as potential agents for managing V. velutina as they produce volatile compounds that attract the hornet.

Comparative Analysis of Callus Induction and Plant Regeneration Rates Using One-step and Two-step Cultures for Rice Anther Cultivation (벼 약배양 1단계 및 2단계 배양을 이용한 캘러스 유도 및 식물 재부화율 비교 분석)

  • Park, Young-hie
    • Journal of Life Science
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    • v.31 no.4
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    • pp.385-388
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    • 2021
  • Anther cultivation for crop breeding is a method of rapid production of homozygosities by greatly reducing the time required for at least six generations to develop new varieties using conventional breeding methods. This technique of producing anther culture provides an opportunity to obtain more green plants from a methodological point of view, and the techniques that save time and effort in anther culture are also important because they increase the efficiency of culture. This study compared the callus induction rate and green plant regeneration rate of a one-step and a two-step culture that differ in their culture media and culture methods. One-step culture allows callus induction and plant regeneration in one medium, whereas two-step culture requires induction and plant regeneration in two different media. In this study, we compared the callus induction and plant regeneration rates of rice anthers as one-step and two-step cultures. The callus formation rate was 13.0% for one-step cultures and 8.6% for two-step cultures, so the rate was 4.4% higher for one-step cultures than for two-step cultures. The plant regeneration rate was 1.0% in one-step cultures and 3.0% in two-step cultures, so the regeneration rate was three times higher for the two-step cultures than for one-step cultures. This suggests that the two-step cultures are more efficient than the one-step cultures for haploid production.