• Journal of Embryo Transfer
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• v.21 no.2
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• pp.157-162
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• 2006

It is well known that unidentified factors in sera, hormones and growth factors promote the proliferation of granulosa cells and nuclear maturation of bovine COCs (cumulus oocytes complexes) in vitro. Attempts had been developed the simple composition of culture media and similar system to in vivo conditions has been applied. In the present study, we investigated the effect of FGF (fibroblast growth factor) on in vitro maturation and in vitro development of Hanwoo COCs. When the COCs were matured in HPM 199 (Inst. of Functional peptide, Japan) containing 0.1, 1 and 10 ng/ml FGF for 24 hr, maturation rates to metaphase II ($70.0{\sim}75.0%$) were significantly higher (p<0.05) than that of control group (0 ng/ml FGF, 37.5%). When matured COCs with FGF were cultured in maturation medium after in vitro fertilization, developmental rates to blastocysts were 9.5, 0 and 2.9%, respectively, compared to 25.0% of the control group (p<0.05). When the matured COCs with FGF were cultured in HPM 199 (IFP971, Inst. of Functional peptide, Japan) containing 10% FBS, 0.8% BSA or 0.1% PVA (polyvinyl alcohol), the blastocyst formation rates were 12.4, 12.8 and 8.5%, respectively, while the rates of matured COCs with FGF and cultured with IVMD and IVD (Inst. of Functional peptide, Japan) without serum were 38.4% and 34.8%, respectively (p<0.05). These results suggested that FGF is available for in vitro maturation of bovine COCs and is not suitable for in vitro development, but further investigation would be need for finding the synergistic autocrine/paracrine fashion of other growth factors in early bovine embryo development.

• Horticultural Science & Technology
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• v.30 no.4
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• pp.437-441
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• 2012

This study was conducted to find out the optimum container for increasing acclimatization rate of in vitro mass propagated plantlets of Ever-bearing strawberry (Fragaria ${\times}$ ananassa Duch.) via bioreactor. Four types of containers were used such as transparent plastic container (TPC), plug tray (PT), I-pot (IP), and black vinyl pot (BVP). Number of date maintaining soil water content above 10% was five days in TPC, three to four days in BVP, two days in PT, and one day in IP. Survival rate of plantlets was 80% in BVP, 70% in TPC, 55% in IP, and 15% in PT. In TPC, growth increment of plantlets was the greatest among all the tested containers and the lowest in IP. Numbers of runner per plant were 3.3 in BVP, 2.9 in TPC, 1.6 in PT, and 1.2 in IP. Total cost was 44,405,300 won/10 a in BVP, resulting in reducing more 6,659,400 won/10 a than IP's (51,064,700 won/10 a). Around 102,718 plants/10 a were produced by using BVP, suggesting that 30,265.1 plants/10 a more could be produced than IP (72,452.9 plants/10 a). Production cost per plant was 432.3 won in BVP, resulting in reducing 272.5 won than IP's (704.8 won). As a result, BVP was appropriate for acclimatization of in vitro plantlets through bioreactor system.

• Journal of Life Science
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• v.20 no.6
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• pp.907-913
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• 2010

Human enteroviruses (HEV) are considered one of the major infectious causes of central nervous system infections such as aseptic encephalomeningitis in pediatrics. This study was focused on providing information related to genetic characteristics and diversities of HEV which prevailed between 2007 and 2009 in Busan, Korea. A total of 2,743 specimens were collected from children and screened for isolation of HEV by cell culture and RT-PCR. Among the specimins, 240 isolates were grouped into 21 different HEV serotypes using VP1 RT-PCR. The major etiological agents were CV-A6 and CV-B2 in 2007, E-6 and E-30 in 2008 and CV-B1 in 2009. The occurrence of HEV infections was the most frequent in the summer (May to August, 188 cases, 78.3%). Most of the isolates were identified from specimens from children under 10 years old, with the highest occurrence in the 2 to 4 year old range (15.2%). However, there were no significant differences between male and female children for the isolates. For analyzing genetic characterization, VP1 gene was amplified by RT-PCR and sequenced. The phylogenetic tree was established by Clustal W method using DNASTAR software. Using the sequence analysis of the VP1 region, it was classified into 2 groups; HEV-A and HEV-B. The HEV-A group contained 6 serotypes and sequences of 31 isolates were compared within each serotype. The HEV-B group contained 10 serotypes and the sequences of 41 isolates were compared within each serotype. Homology analysis of the VP1 region showed that the identity scores of HEV-A and B isolates were different. In conclusion, genetic divergences were observed among the isolates from children between 2007 and 2009 in Busan.

• Journal of the Korean Geographical Society
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• v.31 no.4
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• pp.685-698
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• 1996

Southern Louisiana has one of the largest Vitnamese refrgee neighborhoods after the mid-1970s. It is impressive that one of their adaptive strategies comes from their religious lives which are centered on either Catholicism or Buddhism. The Buddhism community, especially, exhibits an exotic symbolic system of value and attitude, and thus contributes to cultural diversity in the adopted country. The landscape of the Buddhist temple is a visible symbol to them that the host socirty accepts their maintenance of their own cultural identity and that they are also an integral part of American society. Their making-place and being-in-place procedures, although their culture is being transformed in the original shape, put an emphasis on interaction with the host xociety. These procedures have been facilitated by consolidating their identity as a minority group as well as by interacting with the host society. The on-going influx of foreign immigrant groups seems not to drive them to assimilate into the melting-pot society, but to contribute to contribute to the increase in the cultural diversity of the United States.

• Korean Journal of Food Science and Technology
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• v.31 no.1
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• pp.238-245
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• 1999

Antioxidative effects of the water or ethanol extracts from Rhus verniciflua Stokes (RVS) were measured by protection against hydroxyl radicals in mouse brain tissue culture. In the water extracts from RVS, cell viabilities were estimated 60.0, 66.0, 72.0, 84.0 and 90.0% at addition of 1, 2, 4, 7 and $10{\mu}L$, respectively, compared with GO (20 mU/mL) alone. The cell viability in the ethanol extracts was similarly with water extracts. In the antitumor effects, the results showed that percentages of the HeLa cell death were approximately 24% for 12 hrs, 57% for 48 hrs at addition of 10%/well ethanol extracts respectively. To know inhibition of tumor growth, in vivo, mice (BALB/c) were inoculated with 0.25 mL CT-26 $(1{\times}10^6\;cells/mL)$ subcutaneously. After the generation of tumor, the results of RVS extracts (ethanol, water) injection showed generally that the tumor size in BALB/c was reduced. For physicochemical characterization of the RVS extracts, purified substances of water or ethanol extracts were analized with SDS-PAGE and ICP spectrometer. In electrophoresis, gel showed 2 bands (210, 230 KDa). The results of ICP verified that RVS extracts contain $Cu^{2+}$ in both samples. Conclusively, this substance might be a laccase which has a biological effective function, as a natural bioactive substance.

• Journal of the Korean association of regional geographers
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• v.7 no.1
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• pp.67-82
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• 2001

Recently, local traditions and cultural assets have been rediscovered and developed as resources in local societies of Korea and a great deal of effort has been made to develop event tourism. Based on this renewed interest, this study chose as a case the Wang In Cultural Festival in Kurim-ri, Yongam, and examined the background of the early Wang In Cultural Festival and its evolution up to the present focusing on. the festival program. In Cultural Festival first began as the Wang In Cherry Blossom Festival in 1993 with a budget of 50 million won, and has been developed into a large-scale festival with a budget of 340 million won, attracting 500,000 visitors in 2000. Together with such evolution, there has been a great change in the organization and content of the Festival program. The most important change began in the 1997 which included a religious service for Confucian scholars called 'Chunhyang Grand Festival for Dr. Wang In' as a core program. Since then, festival programs related to Dr. Wang In have greatly increased in number. A decisive change became prominent when the festival promotion system was transferred from the Kunseo Young Men's Association during the early period(1993-1996) to Youngam-gun in 1997. It is true that the Wang In Cultural Festival, which began as a purely regional festival initiated by a non-governmental organization, changed a lot in terms of its scale and program when Youngam-gun emerged as a strong leading group in 1997. However, not enough consideration has been given to institutional devices and procedures that are required for the local culture to take a firm root in the community through the festival. Therefore, in order to solve such fundamental problems, a permanent non-governmental organization is required for festival preparation. In addition, in order to promote overall progress of the community and an increase in the quality of the cultural environment of the local society through the festival, it is necessary to discover and promote recognition of cultural groups in the community.

• The Korean Journal of Pesticide Science
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• v.9 no.1
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• pp.70-80
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• 2005

To evaluate the exposure of molinate in agricultural environment and its effect against the non-target crop in air, this experiment was conducted to elucidate volatilization characteristics of molinate in aquatic condition and to determine critical concentration of molinate in the air causing phytotoxicity to Chili pepper. Cumulative volatilized rate of molinate from water was 22.7% at $35^{\circ}C$ for water temperature and 20 L/min for air velocity while 3.2% at $25^{\circ}C$ and 10 L/min within 47 hour after applied under closed system, respectively. The molinate concentrations in air above 60 cm height from soil surface of valley and open paddy rice field were reached the highest value of 18.17 and $11.59{\mu}g/m^3$, respectively within 24 hours after applying granular formulation at dose rate of molinate 150 g/1,000 $m^2$. However, their concentrations were drastically diminished to around 0.18 and $0.51{\mu}g/m^3$ level in 20 days after application, which volatilization pattern were similar to both regions. Also, the concentration of molinate in air above 60 cm height from soil surface was distributed higher 2 times than that above 180 cm height. Meanwhile, a phytotoxic symptom against the nearby chili pepper was revealed within three days after applied and molinate was detected $0.004{\sim}0.006$ mg/kg level from severe damaged leaves. The dose and exposure relations of molinate in the air against the non-target crop was also investigated in lab trial. The phytotoxic symptom, shriveled leaves, of the chili pepper was encountered by exposing two days with concentration of $13.6{\mu}g/m^3$, three days with $6.8{\mu}g/m^3$ or four days with $3.4{\mu}g/m^3$. The symptom was still recovered within four weeks after the plants had received fresh air. On the other hand, the phytotoxic response through root uptake of the herbicide in water culture was relatively insensitive, in which the symptom is observed ten days with the concentration of 300 ${\mu}g/L$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.4
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• pp.395-407
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• 2002

A 3D hydrodynamic-ecological coupled model was applied to estimate a food supply to oysters in Geoje-Hansan Bay where is one of the oyster culturing sites in Korea, In this study, the primary productivity (PP) was adopted as an index of food supply, and the spatial patterns of average chlorophyll a concentration during a culturing seasons from September to May of the following year were simulated by the model, The numerical result showed that PP was high in the inner part of the bay and the adjacent areas of Hwado island, but low in the outer. This result indicates that PP is essentially influenced by anthropogenic nutrient loadings in the system. The model was calibrated using the field data in May which is non culturing season of oysters and a simulated phytoplankton biomass agreed fairly well with the observed data ($R^{2}=0.70$, $RE=10.3\%$). The computed food supply varied from 0.19 to $1.27\;gC/m^{2}/day$ with a mean value of $0.62 gC/m^{2}/day$ from September to May. The highest value was showed in May ($1.27 gC/m^{2}/day$) and the lowest was in February ($0.19 gC/m^{2}/day$).

• Korean Journal of Fisheries and Aquatic Sciences
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• v.4 no.3_4
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• pp.113-120
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• 1971

In 1971, about 150,000 fingerlings of grass and silver carps were produced and distributed from the Fishculture Laboratory of Pusan fisheries College. The adults Were those transplanted from Japan in 1963. Findings during the process of this production are summarized as following : 1. Brood fish must be handled with a great care during the catching, hormone injection and maturity inspection so as the fish are not injured, otherwise, the eggs would not mature perfectly. Scale fall also affects significantly. 2. The amount of pituitary to be injected is preferable to be 2 to 3 times or more in donor's body weight to obtain good results. 3. The eggs should be spawned or stripped and inseminated as soon as mature. If the spawning is delayed the eggs become overmature and the hatching rate decreases significantly. 4. The water once used for the incubation of eggs should not be reused. The eggs under hatching process were melted away when water once used for incubation was reused. 5. A great care must be paid to keep water in quality when the net cage culture system is employed for raising fry or early fingerlings of Chinese carps. The best method to keep water in quality is to supply water in the cage continuously through a pipe or hose. 6. Heavy outbreaks of Spirogyra occured when clear well water was supplied into the cage, and a great number of fry or fingerlings were trapped or entangled resulting in a significant decrease of fish under rearing. It was prevented when moderately bloom-ed pond water was supplied into the cage. 7. Silver carp fry are usually captured and transported in the cool season because they can not be handled in the warm season owing to a high mortality, but cage cultured small size fingerlings ranging from 1.5 to 2 cm in total length are easily handled and transported even in the hot summer season, thus it enables the fish farmers to start growing them one season earlier.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.37 no.3
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• pp.134-147
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• 2019

The purpose of this study was to analyze the functional unit system of NCS landscape field for correction and supplementation of NCS landscape field and the contents of the four-year college landscape course subject. First, 24 unconsolidated four-year universities were selected, and FGI was conducted and verified for 816 courses in 24 universities. The results of the study are summarized as follows, with three sections three, nine divisions and 65 sub-category. First, landscape design subjects accounted for 40.0% of the subjects organized by four-year universities. In addition, the ratio of 12.9% for ecological landscape, 11.3% for landscape construction, 10.2% for others, 10.0% for landscape information, 6.6% for landscape culture and 3.7% for landscape management was surveyed. Balanced and efficient modification and reinforcement of NCS is required in the future. Second, 10(18.9%) units with matching NCS performance criteria and educational objectives were found to be capable of different units(18.9%), 15(28.3%), and 37subjects with inconsistent NCS unit capability (56.9%). Third, looking at the criteria for the reference of each unit of capability presented by the NCS, it is deemed that one unit of capability should be organized separately to improve the practical ability, since it includes the contents of basic knowledge learning. Fourth, the objectives pursued on the basis of the contents of the NCS capability unit and four-year college curriculum were developed by focusing on the development of unit capabilities in the field of landscape construction and landscape management compared to the field of landscape design. It has been shown that a balance is needed for future development. This study is intended to put forward further research that re-examine specific curriculum assessment criteria that have not been classified in the course of classifications based on the curriculum handbook, which excludes interferences from each school.