• 생명과학회지
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• 제14권2호
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• pp.235-238
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• 2004

The possibility for developing multistage culture system to cultivate larvae by computer simulation with basic experiments was investigated. This culture system was composed of 3 stages. At the 1st stage, Chlorella sp. were cultivated and at 2nd stage Chlorella sp. were supplied to rotifer (Brachionus plicatilis), and rotifer were supplied to larvae at the 3rd stage. In this study, Chlorella sp. were cultivated by batch culture to search for the possibility of continuous feeding to rotifer at 2 nd stage. The maximum specific growth rate ($\mu$$_{max}$) of Chlorella sp. at the logarithmic phase was 0.56 [1/day]. Rotifer was cultivated by fed-batch culture at the feeding rate of Chlorella sp., $10^3$, $10^4$, $10^{5}$ and $10^{6}$ [cells/rotiferㆍh] to search for the relation between the feeding rate of Chlorella sp. and the growth rate of rotifer. As the results, the minimum feeding rate of Chlorella sp. was 2.8 ${\times}$ $10^4$ [cells/rotiferㆍday] in the multistage culture system, then the change of rotifer concentration at 2nd stage was simulated by computer. The required amount of rotifer for the growth of larvae was also increased as the growth of larvae. On the 9th day of the culture, the rotifer uptake rate of larvae was 250 [cells/rotiferㆍday]. Based on these basic experiments and results, It was suggested that the possibility of multistage culture system to cultivate larvae with continuous feeding of Chlorella sp. and rotifer.r.

• 한국수정란이식학회지
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• 제27권3호
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• pp.189-192
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• 2012

Although embryonic stem cells (ESCs) or ES-like cells are reported from many mammalian species other than the mouse, the culture system for murine ESCs may not be suitable to the other species. Previously many other research groups have modified either human or mouse ESC culture systems for bovine ESC culture. In this study, we compared three different culture mediums consisting of DMEM, ${\alpha}$-MEM or KnockOut$^{TM}$-DMEM (KO), which are modified from human or mouse ESC culture system, for the generation of bovine ESCs. In this study, some pre-requisite events which are important for establishment and long-term propagation of ESCs such as inner cell mass (ICM) attachment on feeder cells, primary colony formation and sustainability after passaging. Once the ICM clumps attached on feeder cells, this was designated as passage 0. In regards to the rate of ICM attachment, ${\alpha}$-MEM was superior to the other systems. For primary colony formation, there was no difference between DMEM and ${\alpha}$-MEM whereas KO showed lower formation rate than the other groups. For passaging, the colonies were split into 2~4 pieces and passed every 5~6 days. From passage 1 to passage 3, DMEM system seemed to be appropriate for maintaining putative bovine ESCs. On the other hand, ${\alpha}$-MEM tended to be more suitable after passage 6. Although ${\alpha}$-MEM support to maintain a ES-like cell progenies to passage 15, all three culture systems which are modified from human or mouse ESC culture media failed to retain the propagation and long-term culture of putative bovine ESCs. Our findings imply that more optimized alternative culture system is required for establishing bovine ESC lines.

• Design & Manufacturing
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• 제15권2호
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• pp.11-16
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• 2021

Recently, three-dimensional (3D) cell culture systems, which are superior to conventional two-dimensional (2D) vascular systems that mimic the in vivo environment, are being actively studied to reproduce drug responses and cell differentiation in organisms. Conventional two-dimensional cell culture methods (scaffold-based and non-scaffold-based) have a limited cell growth rate because the culture cannot supply the culture medium as consistently as microvessels. To solve this problem, we would like to propose a 3D culture system with an environment similar to living cells by continuously supplying the culture medium to the bottom of the 3D cell support. The 3D culture system is a structure in which microvascular structures are combined under a scaffold (agar, collagen, etc.) where cells can settle and grow. First, we have manufactured molds for the formation of four types of microvessel-mimicking chips: width / height ①100 ㎛ / 100 ㎛, ②100 ㎛ / 50 ㎛, ③ 150 ㎛ / 100 ㎛, and ④ 200 ㎛ / 100 ㎛. By injection molding, four types of microfluidic chips were made with GPPS (general purpose polystyrene), and a 100㎛-thick PDMS (polydimethylsiloxane) film was attached to the top of each microfluidic chip. As a result of observing the flow of the culture medium in the microchannel, it was confirmed that when the aspect ratio (height/width) of the microchannel is 1.5 or more, the fluid flows from the inlet to the outlet without a backflow phenomenon. In addition, the culture efficiency experiments of colorectal cancer cells (SW490) were performed in a 3D culture system in which PDMS films with different pore diameters (1/25/45 ㎛) were combined on a microfluidic chip. As a result, it was found that the cell growth rate increased up to 1.3 times and the cell death rate decreased by 71% as a result of the 3D culture system having a hole membrane with a diameter of 10 ㎛ or more compared to the conventional commercial. Based on the results of this study, it is possible to expand and build various 3D cell culture systems that can maximize cell culture efficiency by cell type by adjusting the shape of the microchannel, the size of the film hole, and the flow rate of the inlet.

• Animal Bioscience
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• 제37권6호
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• pp.1007-1020
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• 2024

Objective: We increased the nuclear maturation rate of antral follicle derived oocytes by using a pre-in vitro maturation (IVM) culture system and improved the developmental potential of these porcine pathenotes by supplementing with melatonin. Furthermore, we investigated the expression patterns of genes involved in cumulus expansion (HAS2, PTGS2, TNFAIP6, and PTX3) derived from small and medium antral follicles before and after oocyte maturation. Methods: Only the cumulus oocyte-complexes (COCs) derived from small antral follicles were induced with [Pre-SF(+)hCG] or without [Pre-SF(-)hCG] the addition of human chorionic gonadotropin (hCG) during the last 7 h of the pre-IVM period before undergoing the regular culture system. The mature oocytes were investigated on embryonic development after parthenogenetic activation (PA). Melatonin (10^-7 M) was supplemented during in vitro culture (IVC) to improve the developmental potential of these porcine pathenotes. Results: A pre-IVM culture system with hCG added during the last 7 h of the pre-IVM period [Pre-SF(+)hCG] effectively supported small antral follicle-derived oocytes and increased their nuclear maturation rate. The oocytes derived from medium antral follicles exhibited the highest nuclear maturation rate in a regular culture system. Compared with oocytes cultured in a regular culture system, those cultured in the pre-IVM culture system exhibited considerable overexpression of HAS2, PTGS2, and TNFAIP6. Porcine embryos treated with melatonin during IVC exhibited markedly improved quality and developmental competence after PA. Notably, melatonin supplementation during the IVM period can reduce and increase the levels of intracellular reactive oxygen species (ROS) and glutathione (GSH), respectively. Conclusion: Our findings indicate that the Pre-SF(+)hCG culture system increases the nuclear maturation rate of small antral follicle-derived oocytes and the expression of genes involved in cumulus expansion. Melatonin supplementation during IVC may improve the quality and increase the blastocyst formation rate of porcine embryos. In addition, it can reduce and increase the levels of ROS and GSH, respectively, in mature oocytes, thus affecting subsequent embryos.

• 한국지역지리학회지
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• 제12권2호
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• pp.291-304
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• 2006

그 동안 문화정보에 관한 연구는 개별 문화정보가 중심을 이루어 왔으며, 그 연구내용은 종이지도를 이용한 권역 설정에 그 초점이 맞추어 졌다고 볼 수 있다. 그 결과 극히 제한적인 계량적 공간분석과 지도상에 보이는 직감적인 분석은 복잡하고 방대한 문화자료를 체계적이고 과학적으로 정리하고 활용하는데 한계가 있었다. 이러한 문제점을 해결하는 방안으로 지역문화정보에 대한 효율적 관리체계의 구축과 문화정보의 시공간적 역동성을 총체적으로 분석할 수 있는 GIS의 도입은 필수 불가결하다. 특히 오늘날 고도 정보산업에 의해 문화 전달속도가 빨라지고 가치가 다변화된 사회구조에서는 편방향의 정보제공이 아닌 양방향 정보제공이 중시되고 있다. WebGIS에 기반을 둔 지역문화정보시스템은 사용자가 인터넷을 이용하여 다양한 문화정보에 대한 시 공간적인 분석과 통계적 분석을 가능하게 됨으로서 WebGIS를 이용한 호남문화정보시스템은 지역정보에 대한 포괄적인 접근이 용이할 뿐만 아니라 문화 개별요소간의 통계 분석을 통한 새로운 정보의 창출과 지역 간 비교 연구에 기여할 수 있을 것으로 생각한다.

• 복식
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• 제56권5호
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• pp.29-43
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• 2006

The purpose of this study is to examine a unique characteristic of the colors of the costumes in Korean basic culture in the aim of seeking the characteristics and the conceptual meanings of colors found in the majority’s culture. The scope of the basic culture was divided into folk belief, folk game and folk play. Within these limits, the colors of the dress, accessories, instruments were extracted by comparing with the naked eye in NCS Color System. For the analysis of hue and tone, the secondary dimensional analysis using NCS color system and the three-dimensional analysis using the software, COLOR 3D Version 2.0, were done. The result of this investigation is that the colors of the costume in the Korean basic culture are white, gray and black of achromatic color and yellow, yellowish red and purplish blue. This confirms that the colors based on Five-elements color are becoming the basis too basic culture. And Arche-pattern, which is a characteristic commonly found in the Korean traditional society, was shown as a characteristic of color. The colors of the costumes in Korean basic culture are uniquely adopted by the Korean civilians according to their religious and philosophical living standard. This study is meaningful in seeking a root for the formation of their unique color culture.

• 한국동물학회지
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• 제22권4호
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• pp.175-183
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• 1979

포유류 동물 난자의 제외배양법이 발달되어 온 이래 趙 (1974)에 의한 미세관내 배양방법이 여러가지 장점을 가지고 있어 널리 이용되고 있다. 본 연구는 이 방법을 보다 간편하게 이용하는 방법과 이 방법을 이용하여 수송가능성을 제시하고저 행하였다. 본 연구의 결과, 사용한, simple culture device는 체외배양법으로서 매우 경제적인 한편 배양의 결과는 매우 양호하였다. 본 방법으로 배양중에 있는 난자의 수송이 가능함을 제시하였고, 난자의 성숙및 초기배아의 분화과정에 대한 연구를 손쉽게 여러 연구실에서 행할수 있을 것으로 기대된다.

• 융합정보논문지
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• 제9권10호
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• pp.154-162
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• 2019

정보통신 기술이 빠르게 발전하면서 사회와 문화 전반에 많은 영향을 미치고 있음에도 전통문화에 대한 체계적인 분류 및 최신 검색 기능을 제공하는 시스템은 아직까지 제공되고 있지 않다. 따라서 본 논문은 전통문화 콘텐츠 분류체계를 수립하여 3D 연표를 자동으로 생성하고 시각화함으로써 사용자에게 체계적인 분류 및 검색 기능을 제공함과 동시에 융복합서비스가 가능한 전통문화 검색 시스템을 개발하였다. 본 시스템은 전통문화 콘텐츠를 수집하는 기능, 수집된 전통문화 콘텐츠를 분류 및 저장하는 기능, 저장된 전통문화 콘텐츠에 기반을 두어 3D 디지털 연표를 자동으로 생성하는 기능을 제공한다. 또한, 시스템의 사용성 평가를 위해 시스템 만족도 설문지를 개발하였고 대학생 19명이 참여하여 시스템 사용성을 검증하였다. 실험 결과, 시스템 만족도에서는 모든 문항이 평균적으로 '만족한다'는 결과를 보였다.

• 한국양식학회지
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• 제19권4호
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• pp.242-246
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• 2006

서해안에서 사육시스템 종류에 따른 참전복 치패의 성장과 생존율을 구명하기 위해서 2001년 6월부터 8월까지 연구하였다. 참전복 치패의 일간 성장률은 정체식 수조에서 $148.6{\pm}48.96\;{\mu}m$로 가장 높게 나타났으며, 살수식 수조에서 $95.3{\pm}21.45\;{\mu}$로 가장 낮게 나타났다. 생존율은 살수식 수조보다 회전식 수조에서 높게 나타나 유의한 차이를 나타냈으며(P<0.05), 회전식 수조에서는 50.0%의 높은 생존율을 보인 반면, 정체식 수조에서 38.3%로 가장 낮았다. 본 연구결과는 회전식 수조에서 치패의 성장과 생존율이 가장 높게 나타났다.

• 한국수정란이식학회지
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• 제28권3호
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• pp.273-280
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• 2013

The aim of this study was to establish a three dimensional (3D) culture system of endometrial cells and to examine the plasminogen activators (PAs) activity in porcine uterine. The 3D culture system in porcine endometrial cells was composed to mixture 3D gel, stromal cells and epithelial cells. The 3D culture system was used to identify normal structure search as uterine tissue and PAs expression in this study. In results, porcine endometrium epithelial cells forming a top monolayer and endometrium stromal cells developed as fibroblast-like within 3D matrix scaffold. Expression of urokinase-type PA (uPA) and tissue-type PA (tPA) were observed during the 3D culture using immunofluorescence. PA activity in 3D-cultured endometrial cells was no significant difference between the tissue type, but 2D culture system were significantly lower than in 3D-cultured endometrial cells (P<0.05). Therefore, basic system and functional aspect of 3D culture could be established with similar system of endometrium tissue. We suggest that this study was assumed applicable as baseline data to investigate mechanism between porcine uterus cells in vitro.