• 제목/요약/키워드: Culture system

검색결과 6,098건 처리시간 0.047초

Hydrogen Metabolism in Clostridium acetobutylicum Fermentation

  • J.Gregory Zeikus
    • Journal of Microbiology and Biotechnology
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    • 제2권4호
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    • pp.248-254
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    • 1992
  • The initial growth of Clostridium acetobutylicum was not inhibited by 1 atm of H$_2$ while H$_2$ reduced glucose consumption in a solventogenic culture of a phosphate limited 2-stage chemostat. Under 1 atm of H$_2$, a solventogenic culture consumed hydrogen, but an acidogenic culture produced hydrogen. H$_2$ consumption by the solventogenic culture was enhanced by the addition of 5 mM neutral red, an artificial electron carrier with a redox potential of -325 mV. Hydrogenase activity, measured in both directions of production and consumption, showed that activity coupled with methyl viologen is higher in an acidogenic culture than in a solventogenic culture, and that the two cultures have similar activities for methylene blue reduction. The solventogenic culture showed a higher activity coupled with neutral red than the acidogenic culture. From these results, it is hypothesized that hydrogen producing hydrogenase activity is high during the acidogenic phase, and decreases as solventogenesis starts, and that the solventogenic culture produces a second hydrogenase which uses an electron carrier other than ferredoxin. This hypothesis was supported by the fact that enzyme activities involved in electron flow can be coupled to neutral red, indepedent of ferredoxin, and that neutral red addition to the fermentation system increased butanol yield, with a decrease in production of less reduced fermentation products, and $H^2$.

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안전프로그램(Safety Program) 및 안전문화(Safety Culture)에 대한 조사 (A Study on the Perception of Safety Program)

  • 길호성;이학봉;송병흠
    • 한국항공운항학회지
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    • 제27권3호
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    • pp.82-89
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    • 2019
  • There are many ways to identify airline's perception of the safety program and safety culture. In particular, various studies are under way to measure airline safety culture and safety awareness of airline employees. Often, survey methods are used a lot, but there is a limit to understanding the complex and diverse aviation culture through surveys alone. Nevertheless, the Air Safety Management System (SMS) of the International Civil Aviation Organization (ICAO) emphasizes the importance of safety surveys as a means of ensuring awareness of safety culture. The safety surveys is effective in identifying and providing awareness of the relationship between employees and the Air Safety Program (SMS, Safety Management and Safety Culture aspects). In this study, we conducted a survey of Z Airlines flight attendants and cabin crew to compare their perception of safety programs and culture, and based on this survey, we would like to compare and analyze simple safety culture measurements and safety awareness.

Insulin enhances neurite extension and myelination of diabetic neuropathy neurons

  • Pham, Vuong M.;Thakor, Nitish
    • The Korean Journal of Pain
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    • 제35권2호
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    • pp.160-172
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    • 2022
  • Background: The authors established an in vitro model of diabetic neuropathy based on the culture system of primary neurons and Schwann cells (SCs) to mimic similar symptoms observed in in vivo models of this complication, such as impaired neurite extension and impaired myelination. The model was then utilized to investigate the effects of insulin on enhancing neurite extension and myelination of diabetic neurons. Methods: SCs and primary neurons were cultured under conditions mimicking hyperglycemia prepared by adding glucose to the basal culture medium. In a single culture, the proliferation and maturation of SCs and the neurite extension of neurons were evaluated. In a co-culture, the percentage of myelination of diabetic neurons was investigated. Insulin at different concentrations was supplemented to culture media to examine its effects on neurite extension and myelination. Results: The cells showed similar symptoms observed in in vivo models of this complication. In a single culture, hyperglycemia attenuated the proliferation and maturation of SCs, induced apoptosis, and impaired neurite extension of both sensory and motor neurons. In a co-culture of SCs and neurons, the percentage of myelinated neurites in the hyperglycemia-treated group was significantly lower than that in the control group. This impaired neurite extension and myelination was reversed by the introduction of insulin to the hyperglycemic culture media. Conclusions: Insulin may be a potential candidate for improving diabetic neuropathy. Insulin can function as a neurotrophic factor to support both neurons and SCs. Further research is needed to discover the potential of insulin in improving diabetic neuropathy.

조직문화와 이직 관계에 대한 실증적 고찰: 개인특성의 조절효과를 중심으로 (Organizational Culture and Turnover: Focusing on Moderating Effects of Personal factors)

  • 안관영
    • 산업경영시스템학회지
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    • 제20권44호
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    • pp.343-356
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    • 1997
  • Many empiric research findings have supported that the characteristics and strength of Organization Culture(OC) is closely related with organizational performance(absenteeism, job satisfaction, cohesiveness and turnover etc.). The first purpose of this study is to review the relationship between OC and turnover as a performance factor, and the second and primary purpose is to test the moderating effects of personal factors(sex, educational career, rank, occupation, marital status, job switching, age, company tenure and job tenure) between OC and turnover. For the analytical purpose of this article, OC type is categorized as Maintainable Culture(MC), Affiliation Culture(AC), Progressive Culture(PC) and Rational Culture(RC) based on Quinn(1985). The empirical results are as followings: Moderating effects are found in sex(with MC and RC), educational career(with MC, PC and RC), job switching(with MC and RC).

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양액재배 오이의 급액제어모델 개발에 관한 기초연구 (A Fundamental Study on the Development of Irrigation Control Model in Soilless Culture of Cucumber)

  • 남상운;이남호;전우정;황한철;홍성구;허연정
    • 한국농공학회:학술대회논문집
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    • 한국농공학회 1998년도 학술발표회 발표논문집
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    • pp.224-229
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    • 1998
  • This study was conducted to develop the simple and convenient irrigation control model which can maintain the appropriate rates of irrigation and drainage of nutrient solution according to the environmental conditions and growth stages in soilless culture of cucumber. In order to obtain fundamental data for development of the model, investigation of the actual state of soilless culture practices was carried out. Most irrigation systems of soilless culture were controlled by the time clock. Evapotranspiration of cucumber in soilless culture was investigated and correlations with environmental conditions were analyzed, and its prediction model was developed. A irrigation control model based on the time clock control and there were considered seasons, weather conditions, and growth stages was developed. Applicability of the model was tested by simulation. Drainage rates of irrigation system controlled by conventional time clock, integrated solar radiation, and the developed model were 61%, 20%, and 32%, respectively in cucumber perlite culture.

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양액재배 급액제어모델 개발에 관한 기초연구 (A Fundamental Study on the Development of Irrigation Control Model in Soilless Culture)

  • 남상운
    • 한국농공학회지
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    • 제41권2호
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    • pp.37-43
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    • 1999
  • This study was conducted to develop the simple and convenient irrigation control model which can maintain the appropriate rates of irrigation and drainage of nutrient solution according to the enviornmental conditions and growth stages in soilless culture of cucumber. In order to obtain fundamental data for development of the model, investigation of the actual state of soilless culture practices was carried out. Most irrigatioin systems of soillness culture were controlled by the time colock. Evapotranspiration of cucumber in soilness culture was investigated and correlations with environmental conditions were analyzed , and its estimating model was developed. In order to develop the irrigation system which can control the amount of nutrient solution applied according to seasons, weather conditions, and growth stages, a irrigation clock control was developed. Applicability of the model was tested by simulation. Drainage rates of nutrient solution controlled by conventional time clock, integrated solar radiation, and the developed model were 61% , 20%, and 32% , respectively in cucumber perlite culture.

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기업문화가 조직효과성에 미치는 영향 -A지하철을 중심으로- (A Study on the Effect of Corporate Culture on the Organizational Effectiveness. - Focusing on the Case of A Subway -)

  • 신택현
    • 한국철도학회논문집
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    • 제8권2호
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    • pp.176-181
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    • 2005
  • The purpose of this thesis is, based on the cognitive response of employees, to depict the phenomenon and the pattern of organizational culture in A Subway system, To attain this purpose, several aspects of organizational culture are surveyed through questionaire and analyzed by SPSS. The major findings are . 1) In general, the cognitive response of employees in railway-concerned firms on culture-related aspects is awfully negative. 2) Job satisfaction and involvement in A Subway is highly related to achievement factor in terms of culture. 3) According to the analysis based on four kinds of Job Groups, certain job groups such as drivers and vehicle maintenance are seen as having exceptionally negative attitude towards every aspects of their organization. So, some careful diagnosis and approaches to the symptoms, the causes, and the treatments are urgently needed.

안전문화 평가방안 연구 (Towards an Effective Assessment of Safety Culture)

  • 홍인기;백종배
    • 한국안전학회지
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    • 제31권1호
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    • pp.118-125
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    • 2016
  • The purpose of this study is to identify issues for an effective safety culture assessment by conducting a case study of an electronics manufacturing plant in Korea. Cooper's safety culture model was used as the assessment method, and Fleming and Hudson's safety culture maturity models were applied as assessment criteria. The results of the safety culture assessment showed that there needs to be a design optimized for study purposes. For example, the correlation between the questionnaire survey and in-depth interview needs to be analyzed. The result of the behavior monitoring should show the relationships with other dimensions. A safety culture maturity model has to be developed to customize the study factors and questions.

클라우드 시스템 기반의 콘솔 게임에 관한 연구 (A Study on the Consol Game Based on the Cloud System)

  • 김판관;박석영;이영훈;조현규;강민주;김효남
    • 한국컴퓨터정보학회:학술대회논문집
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    • 한국컴퓨터정보학회 2015년도 제52차 하계학술대회논문집 23권2호
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    • pp.238-239
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    • 2015
  • 본 논문에서는 국내 게임 시장 규모에서 다른 부분에 비해 떨어지는 콘솔 게임 시장의 발전방향으로 클라우드 기반의 콘솔게임기술에 대한 내용 제안한다. 콘솔게임의 새로운 패러다임의 방향은 사람들이 콘솔의 가장 큰 단점으로 꼽은 기기의 가격으로 인한 경제적인 부담을 덜어내며 부피의 간소화의 효과 또한 기대한다. 이러한 기대로 단점을 보완하여 콘솔 게임을 대중화 시킨다면 자연스레 시장의 확장으로 이어지며 이러한 시장 확장 상황에서 기업들의 정책 방향을 연구할 수 있을 것으로 기대한다.

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계획적 중소기업문화의 조성에 관한 연구 (A Study on the Cultivation of the Small-Medium Size Corporate Culture)

  • 이원희
    • 산업경영시스템학회지
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    • 제18권33호
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    • pp.55-61
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    • 1995
  • The corporate culture contributes much to the approach to the management problems as well as to the view to see the problems in the light of the multiplying-enterprise environment. In comparision with the large size corporates, the small-medium size corporates under various disadvantage conditions increase to the high achievement through the development of corporate culture, though the period and method to root in the corporate culture differ from one another depending on the situations. The purpose of this paper is to propose the owner's intention and efforts, the development of the corporate culture helpful for the long-run achievement, the cultural network and the member's role which prove to be effective method to root in the corporate culture.

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