• Journal of Aerospace System Engineering
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• v.2 no.3
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• pp.7-11
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• 2008

This is a study to research the effective way to enhance the performance of safety management by gathering and analyzing the information of undesirable occurrences that may result in accident or serious incident. This includes the way to identify the potential hazards related with the proactive activities. As detailed improvements, this paper introduces the mandatory and voluntary reporting system, normal operation safety survey, ATC quality assurance and the encouragement of just culture.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.39-39
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• 2005

Large scale mammalian cell culture has become, over the past two decades, the preferred method to produce therapeutic monoclonal antibodies. In this presentation, I will introduce disposable bioreactor system and analyze key factors and points for consideration during mammalian cell culture process development. Example will be provided highlighting the selection of master cell, culture media and environmental factors based on productivity and product quality.

• KSBB Journal
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• v.13 no.5
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• pp.547-553
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• 1998

For the efficient production of a new exo-polysaccharide from Ganoderma lucidum ASI 7004, the optimum conditions and methods in submerged cultivation were investigated with an airlift fermenter system. The optimum aeration rate was 2.5 Wm at the initial pH 5.0 and 28$^{\circ}C$. The increase of dissolved oxygen concentration by pure oxygen supply during cultivation did not improved the exo-polysaccaride production and the mycelial growth. The maximum exo-polysaccharide production and the mycelial growth under the optimum culture condition were obtained in media of glucose 60g/L, yeast extract 6g/L, (NH4)2HPO4 1g/L and KH2PO4 0.5g/L. Under these optimum medium and culture conditions, about 7.15g/L of exo-polysaccharide and 13.9g/L of mycelial growth were producted, respectively.

• Journal of the Korean Society of Food Culture
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• v.20 no.2
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• pp.144-151
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• 2005

This study examined food culture appearing in Giroyeon paintings in the late Chosun Dynasty (early $17^{th}{\sim}l9^{th}$ century), a period under political and economical difficulties in overcoming frustrations and looking for self-restoration methods after Imjinweran$(1592{\sim}1598)$ and Byungjahoran$(1636{\sim}1637)$. Paintings related to Giroyeon include Lee Gi-ryong's $(1600{\sim}?)$ painted in 1629 under the reign of King Injo $(1623{\sim}1649)$, painted jointly by Kim Jin-yeo et al. in 1720, the first year of King Gyeongjong $(1720{\sim}1724)$ and Kim Hong-do's painted in 1804 under the reign of King Sunjo $(1800{\sim}1834)$ in the early $19^{th}$ century. These paintings over three centuries of the late Chosun show changes in the character of Giroyeon, food culture and tableware culture, etc. According to these paintings, the character of Giroyeon was expanded from a feast held for high civil officials to a banquet comprehending general civil servants. These paintings show Hyangeumjueui, which means the decorum of respecting and sowing the aged men of virtue with alcohol beverage, table manner, seated culture represented by cushions and mats and individual-table culture. In addition, the expansion of demand for sunbaikja and chungwhabaikja and the diversification of vessels and dishes are demonstrated in Giroyeon paintings after Imjinweran in 1592. in this way, Giroyeon paintings show the historical characteristics of the late Chosun Dynasty, such as the collapse of Yangban-centered political system, the disintegration of the medieval system due to the emergence of the Silhak and the diversification of food culture.

• Biomolecules & Therapeutics
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• v.2 no.3
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• pp.236-243
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• 1994

A splenocyte culture system supplemented with liver microsomes was developed to detect immunotoxic chemicals which require metabolic activation using cyclophosphamide as a positive standard. When liver microsomes were added to splenocyte cultures isolated from female B6C3Fl mice, the proliferation of splenocytes by lipopolysaccharide (LPS) was increased and the proliferation by concanavalin A (Con A) was decreased. However, when compared with each corresponding control, cyclophophamide was successfully activated to metabolites capable of suppressing Iymphoproliferative responses. This suppression was clearly dependent upon the amounts of microsomes added and/or the concentration of cyclophosphamide exposed. In these cultures, the proliferation of splenocytes was suppressed when the cells were exposed to cyclophosphamide on the day of culture initiation. On the other hand, microsome was responsible for the increase in LPS mitogenicity and NADPH was responsible for the decrease in Con A mitogenicity. Finally, our present culture system was compared with the hepatocyte-splenocyte coculture system which we had developed earlier. We found that the hepatocyte-splenocyte coculture was better able to activate cyclophosphamide to metabolites capable of suppressing the antibody response to sheep erythrocytes. Although our present culture system was relatively poor to activate cyclophosphamide in cultures for antibody response, it will be useful as a simple screening method to detect suppression of certain in vitro immunotoxic parameters like LPS mitogenicity by chemicals which require metabolism.

• Journal of Biosystems Engineering
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• v.15 no.4
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• pp.328-338
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• 1990

The objective of this study was to develop automatic systems of nutrient solution management for optimal nutrient solution environment and labor saving in water culture which enables factory crop production. In this study, an automatic control system and its driving program are developed to prepare, supply, and recover nutrient solution and to keep the optimal solution concentration level using microcomputers. Based on this study, the following conclusions are obtained: 1. The concentration measured by the system using oscillating circuit designed and built in this study, gave good agreements with the actual nutrient solution. 2. In water culture, the period of 12 hours for measuring concentration, pH, and temperature of the nutrient solution was optimum. Addition of control solution due to the decrease of the nutrient solution concentration is required in every 3 to 5 days. 3. It is estimated that the period of the whole solution change is 15 days, however, further research is needed to assure it. In addition, this period must be shortened in the future. 4. Both the hardware and software of the developed optimal nutrient solution control system in the water culture are working very well, however, it is necessary to develop a more economical one-chip micro controller to substitute for the microcomputer.

• Journal of the Korea Safety Management & Science
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• v.17 no.4
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• pp.353-363
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• 2015

In unlimited competitive period changed every time, domestic manufacturing industry feels a crisis between China and Japan, who are leaping to manufacturing strong countries with worldwide manufacturing factories. In this situation, many companies make an effort to learn and introduce Toyota production method drawing innovation of production method and reduction of many costs for high quality, but most of cases were failed. The reason for the failure should be that correct understanding and application of TPS corporate culture were insufficient and job attitude was not considered when applying Toyota production system. Therefore, the purposes of this thesis are to arrange major contents based on relative documents of precedent researchers, conduct a survey for employees of middle and small scaled manufacturers who introduced Toyota production system, analyze changes of enterprises' job attitude by introduction of TPS corporate culture and the effects on company's management results through the various analyses for grasping relationship among TPS corporate culture, job attitude and management results, introduce Toyota production system more effectively and suggest measures to maximize company's long-term management results.

• Advances in Computational Design
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• v.4 no.3
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• pp.307-326
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• 2019

A low computational cost semi-analytical method is developed, based on eigenfunction expansion, to study the vibration of rectangular plates subjected to a series of moving sprung masses, representing a bridge deck under multiple vehicle or train moving loads. The dynamic effects of the suspension system are taken into account by using flexible connections between the moving masses and the base structure. The accuracy of the proposed method in predicting the dynamic response of a rectangular plate subjected to a series of moving sprung masses is demonstrated compared to the conventional rigid moving mass models. It is shown that the proposed method can considerably improve the computational efficiency of the conventional methods by eliminating a large number of time-varying components in the coupled Ordinary Differential Equations (ODEs) matrices. The dynamic behaviour of the system is then investigated by performing a comprehensive parametric study on the Dynamic Amplification Factor (DAF) of the moving loads using different design parameters. The results indicate that ignoring the flexibility of the suspension system in both moving force and moving mass models may lead to substantially underestimated DAF predictions and therefore unsafe design solutions. This highlights the significance of taking into account the stiffness of the suspension system for accurate estimation of the plate maximum dynamic response in practical applications.

• Fisheries and Aquatic Sciences
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• v.19 no.2
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• pp.9.1-9.7
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• 2016

This study was conducted to identify the general conditions for the isolation and in vitro culture of ovary-derived cells in rockfish (Sebastes schlegeli). The effects of three different enzymes on cell retrieval from ovarian tissues were evaluated first, and then the ovary-dissociated cells were cultured under various culture conditions, with varying basal media and culture temperatures, addition of growth factors, and/or culture types. We found that collagenase type I treatment was effective for cell isolation from ovarian tissues. From a total of 42 trials to evaluate the effects of basal media and culture temperatures on cell culture of ovary-dissociated cells, we observed that Leibovitz's L15 medium was more supportive than Dulbecco's modified Eagle's medium for culture, and the cells could grow at all three temperatures tested, 15, 20, and $25^{\circ}C$, at least up to passage 2. However, growth factor addition did not improve cell growth. Introduction of suspension culture after monolayer culture expanded the culture period significantly more than did monolayer culture alone. Our results may provide a basis for developing an in vitro system for S. schlegeli germline cell culture, which will ultimately lead to improvement of the species.

• Journal of Photoscience
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• v.9 no.2
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• pp.5-8
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• 2002

Photoperiodism is an important adaptive phenomenon in various physiological parameters including reproduction to cope with seasonal changes. Involvement of extraretinal photoreceptors in the photoperiodism in non-mammalian vertebrates has been well established. In addition, circadian clock system is known to be involved in the photoperiodic time measurement. The pathway consists of light-input system, time measurement system (circadian clock), gonadotropin releasing hormone (GnRH) production in the hypothalamus, luteinizing hormone (LH) and follicle stimulating hormone (FSH) production in the pituitary, and final gonadal development. Recently, several laboratories reported photopigments newly cloned in the pineal, eyes and deep brain in addition to already known visual pigments in the retina. These are pinopsin, parapinopsin, VA-opsin, melanopsin, etc. All these photopigments belong to the opsin family having retinal as the chromophore. However, the function of these photopigments remains unknown. I reviewed the studies on the location of the photopigments by immunocytochemistry. I also discussed the results on the action spectra for induction of gonadal development in relation with the location of the photoreceptors. Various physiologically active substances distribute in the vertebrate brain. Such substances are GnRH, GnIH, neuropeptide Y, vasoactive intestinal peptide, c-Fos, galanin, neurosteroids, etc. I summarized the immunhistochemical studies on the distribution and the photoperiodic changes of these substances and discussed the route from the deep brain photoreceptor to GnRH cells.