• 제목/요약/키워드: Culture system

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Phytomitogen에 의한 토끼 임파구의 blast transformation I. 유사분열에 미치는 배지, 유사분열촉진물질 및 배양시간의 효과 (Phytomitogen induced blast transformation of rabbit I. Effect of medium, phytomitogen and culture hours on the uptake of 3H-thymidine)

  • 김종수;김충희
    • 대한수의학회지
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    • 제31권1호
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    • pp.49-53
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    • 1991
  • The present study has been carried out to investigate the optimal condition on lymphocyte blastogenesis of rabbit lymphocytes, whole blood culture and microculture system in conjunction with a semiautomatic multiple sample harvester(SAMSH) was used to study the In vitro optimal condition of rabbit lymphocytes. Data were presented to show many variables that are involved in studying the phytohemagglutinin(PHA) and lipopolysaccharide(LPS) response of rabbit lymphocyte in a microculture system. Analysis indicated that the conditions for optimal PHA as measured by incorporation of $^3H$-TdR include: (1) use of RPMI-1640 as culture medium. (2) use of $6{\mu}g$ of PHA, per culture. (3) 48-hours culture period. Conditions for optimal stimulation with LPS mitogen were similar to those used for PHA.

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SEM-based study on the impact of safety culture on unsafe behaviors in Chinese nuclear power plants

  • Licao Dai;Li Ma;Meihui Zhang;Ziyi Liang
    • Nuclear Engineering and Technology
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    • 제55권10호
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    • pp.3628-3638
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    • 2023
  • This paper uses 135 Licensed Operator Event Reports (LOER) from Chinese nuclear plants to analyze how safety culture affects unsafe behaviors in nuclear power plants. On the basis of a modified human factors analysis and classification system (HFACS) framework, structural equation model (SEM) is used to explore the relationship between latent variables at various levels. Correlation tests such as chi-square test are used to analyze the path from safety culture to unsafe behaviors. The role of latent error is clarified. The results show that the ratio of latent errors to active errors is 3.4:1. The key path linking safety culture weaknesses to unsafe behaviors is Organizational Processes → Inadequate Supervision → Physical/Technical Environment → Skill-based Errors. The most influential factors on the latent variables at each level in the HFACS framework are Organizational Processes, Inadequate Supervision, Physical Environment, and Skill-based Errors.

자소(紫蘇)의 조직배양에 관한 연구(II) (Studies on Tissue Culture of Perilla Species)

  • 신순희
    • 생약학회지
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    • 제17권1호
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    • pp.7-11
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    • 1986
  • The young leaf of Perilla species was cultured by two stage culture system using the medium containing mevalonic acid lactone. The growth rate and productivity of essential oil of callus were increased. The essential oil from intact plant and callus was also analysed. Sesquiterpene hydrocarbons and one sesquiterpene alcohol were identified in essential oils of callus.

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Effects of Deep Seawater on the Growth of a Green Alga, Ulva sp.(Ulvophyceae, Chlorophyta)

  • Matsuyama, Kazuyo;Serisawa, Yukihiko;Nakashima, Toshimitsu
    • ALGAE
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    • 제18권2호
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    • pp.129-134
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    • 2003
  • In order to examine the effects of deep seawater (mesopelagic water in the broad sense) on the growth of macroalgae, the growth and nutrient uptake (nitrate and phosphate) of Ulva sp. (Ulvophyceae, Chlorophyta) were investigated by cultivation in deep seawater (taken from 687 m depth at Yaizu, central Japan, in August 2001), surface seawater (taken from 24 m depth), and a combination of the two. Culture experiments were carried out in a continuous water supply system and an intermittent water supply system, in which aerated 500-mL flasks with 4 discs of Ulva sp. (cut sections of ca. 2 $cm_2$) were cultured at 20$^{\circ}C$ water temperature, 100 $\mu$mol photons $m^{-2}{\cdot}s^{-1}$ light intensity, and a 14:10 light:dark cycle. Nutrient uptake by Ulva sp. was high in all seawater media in both culture systems. The frond area, dry weight, chlorophyll a content, dry weight per unit area, and chlorophyll a content per unit area of Ulva sp. at the end of the experimental period were the highest in deep seawater and the lowest in surface seawater in both culture systems. These values, except for dry weight per unit area and chlorophyll a content per unit area, for each seawater media in the intermittent water supply system were higher than those in the continuous water supply system. We conclude that not only deep seawater as the culture medium but also the seawater supply system is important for effective cultivation of macroalgae.

크리티컬 패션에 표현된 패션산업 시스템과 패션소비문화에 대한 비평적 메시지 (Critical Messages on the Fashion Industry System and Fashion Consumption Culture in Critical Fashion Design)

  • 정정희;임은혁
    • 한국의류산업학회지
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    • 제21권6호
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    • pp.717-729
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    • 2019
  • This study defines critical fashion designs and investigate its critical messages on fashion itself. The critical messages on fashion are categorized into two major issues of a fashion industry system and fashion consumer culture. This study contributes to the understanding of meaning and value for critical fashion messages that match critical art. As the research method, this study combines a literature review and case studies and the research scope focuses on cases that have appeared in fashion media since the 2000s when social critical messages in fashion began to emerge. The results of the study are as follows. Critical designers such as Viktor & Rolf, Elisa van Joolen, Issey Miyake, and Mary Ping have delivered messages challenging the nature of fashion industry system that criticize the cycle and limitation of a fashion system and pursues changes in perception of sustainability. The critical message on fashion consumer culture articulated by designers such as Alexander McQueen, Vivienne Westwood, Hussein Chalayan, and Ricarda Bigolin & Nella Themelios insist on the formation of community while delivering a critical message on social, political, and cultural problems that raise the mechanism of social awareness through fashion design.

원자력 규제정책에 대한 국민신뢰도 평가 SD모델 연구 (System Dynamic Model Study of Public Trust on Nuclear Regulation Policy)

  • 곽미애;차현주;김성현;정관용
    • 한국시스템다이내믹스연구
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    • 제16권1호
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    • pp.53-74
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    • 2015
  • The purpose of this paper is to simulate public trust on nuclear regulation policy. The first of all, public trust variables and the model were developed and analysed by system dynamic method. The model are consisted of the operator safety culture level, regulatory competence levels, the public satisfaction and public trust level. The scenario is made up three type which base scenario, the system operator's safety culture level and accident event level. First. the simulation results of standard scenario shows that rapidly declining public satisfaction and trust level of the national safety after Japan's nuclear accident in November 2011. Second, operator safety culture level and simulated divided into three levels. The results showed that a greater impact on the public satisfaction if bad than good case. Finally, the size of the accident was simulated divided into three levels levels(no accident, medium, serious accidents). the results showed a weak effect against the regulatory capacity and safety performance levels but showed a significant impact on public satisfaction and confidence level.

국적항공사 조종사들의 항공안전문화 인식도 변화에 대한 연구 (A Study on Degree of Perception Changes of Korean National Carriers' Pilots in Safety Culture)

  • 문봉섭;김기웅;최연철
    • 한국항공운항학회지
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    • 제23권3호
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    • pp.88-93
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    • 2015
  • Among other various aspects of safety culture, this research has considered safety culture from the view of aviation. A tool to examine aviation safety culture has been developed on the basis of Global Aviation Safety Network's safety culture survey. Using this tool, the degree of perception of Korean national carriers' pilots in safety culture has been examined three times for the period from 2002 to 2015. Compared to an initial result of the survey in 2002, results in 2008 and 2015 demonstrate that safety culture among pilots has rapidly changed from negative and bureaucratic to positive. Hence, it is expected that positive index of safety culture will increase 28% (total approximate 90%) in 2020.

The Effect of Organizational Culture on Innovation Activities and Organizational Effectiveness

  • Kim, Moon Jun
    • International Journal of Advanced Culture Technology
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    • 제10권3호
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    • pp.93-103
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    • 2022
  • The purpose of this study is to empirically analyze the mediating effect of innovation activities on the effect of organizational culture perceived by organizational members on organizational effectiveness. The main results are as follows. First, Hypothesis 1, organizational culture, was adopted as it showed a positive influence on innovation activities. Second, since organizational culture showed statistically significant effect on organizational effectiveness, Hypothesis 2 was adopted. Third, the innovation activity, which is hypothesis 3, was analyzed to have a positive influence on organizational effectiveness. Therefore, hypothesis 3 was accepted. Fourth, the innovation activity, hypothesis 4, showed a mediating effect between organizational culture and organizational effectiveness. As shown in the results of this study, it is indicated that organizations should improve organizational effectiveness for growth and development through the advancement of the sustainability management system in the rapidly changing business environment. To this end, it was necessary to systematically build and activate an organizational culture and innovation activities suitable for the characteristics of the organization. In other words, organizational effectiveness can be improved when constructing and implementing advanced innovation activities based on measures to revitalize organizational culture according to changes in the business environment.

Morphological Variation of Lingulodinium polyedrum (Dinophyceae) in Culture Specimens and Reinterpretation of the Thecal Formula

  • Kim, Keun-Yong;Yoshida, Makoto;Kim, Chang-Hoon
    • ALGAE
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    • 제20권4호
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    • pp.299-304
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    • 2005
  • Morphological observation of a unialgal culture of Lingulodinium polyedrum was conducted under light- and scanning electron microscopes. Lingulodinium polyedrum was redefined as having the thecal formula of APC, Q, 5', 6", 6c, 6s, 5'", 2"" using the reinterpreted tabulation system. In our tabulation system, the Q, which is equivalent to the 3' in the strict Kofoidean system, was treated as an auxiliary platelet because of its considerable morphological variations in culture specimens. Subsequently, apical plates were redefined as plates directly connected to the APC and/or the Q. The 1"' and the 1p in the conventional Kofoidean system were treated as a component of the sulcus (the Ssa) and as an antapical plate (the 1""), respectively. Our reinterpreted tabulation system clearly differentiated extant gonyaulacalean taxa.

A Study of Information System Availability Guarantee Methods and Application

  • Kim, Hee Wan
    • International Journal of Advanced Culture Technology
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    • 제8권3호
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    • pp.292-299
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    • 2020
  • This paper presents an evaluation criteria of an information system availability for guaranteeing availability (service target level) from the perspective of the SLA contract and its technical point of view. In order to verify the effectiveness for information system failure and availability guarantee measures, three cases were examined. In summary, the failure time was reduced by 32% ~ 62% after applying the availability guarantee measure, verifying the excellence in the evaluation of an information system availability.