• Title/Summary/Keyword: Culture maturity

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Comparison of Adventitious Shoot Formation in Petiole Explant Cultures of 20 Cultivars of Catharanthus roseus

  • Lee, Soo-Young;Park, Pil-Son;Chung, Hwa-Jee;In, Dong-Soo;Park, Dong-Woog;Jang R. Liu
    • Journal of Plant Biotechnology
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    • v.5 no.1
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    • pp.59-61
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    • 2003
  • Petiole explants from 20 cultivars of Catharanthus roseus were cultured on various shoot-inducing media to assess their competence for adventitious shoot formation. After eight weeks of culture on Murashige and Skoog' s medium supplemented with 4.4 $\mu\textrm{m}$6-benzyladenine and 0.5 $\mu\textrm{m}$ $\alpha$-naphthaleneacetic acid, petiole explants from 'Cooler Icy Pink' exhibited the greatest frequency of adventitious shoot formation at 40%, which was followed by 'Little Bright Eye'. By comparing with a previous study on assessment of competence for adventitious shoot formation in hypocotyl explant cultures of various cultures, it is indicated that the relative degree of their competence among cultivars varies to the organ used for the source of explant. Excised adventitious shoots were readily rooted on half-strength MS basal medium. Regenerated plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.

Problems and Countermeasures in Applying of Toyota Production System (도요타 생산방식의 도입적용상 문제점과 대응방안)

  • Park, Jin-Je;Lee, Dong-Hyung
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.38 no.1
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    • pp.152-161
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    • 2015
  • Until a recent date, Toyota Production System (called TPS) was introduced by many domestic companies to remove waste and reduce manufacturing cost. However, cases of substantial and effective improvement after the introduction are not much. Even though many companies have actively conducted TPS during that time, the outcome is not satisfactory. In this paper, we show the problems and core contents to consider in applying of TPS as follows. First, the innovative organizational culture formed by active participation of employees and leadership of the CEO is very important for a successful introduction of TPS above all. Second, it is necessary to prepare various training programs optimized for the field in order to continuously improve the competency of employees in each class, and to train skilled personnel through that programs. Third, it is necessary to improve the maturity level of TPS application through the construction of correct evaluation system on accomplishment of the production system. In addition, the problems that occur should be solved through the continuous improvement activities. These results will help to TPS introduction of the domestic small-medium companies. Therefore, this study will contribute to strengthen and improve the global competitiveness in the related industries.

Microscopic Examination of the Suppressive Action of Antifungal Substances from Pseudomonas aeruginosa on Asexual Sporulation of Fungi

  • Kwon S. Yoon;Bu Y. Min;Park, Hyoung T.;Lee, Jong K.;Kim, Kun W.
    • Journal of Microbiology
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    • v.37 no.1
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    • pp.27-34
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    • 1999
  • Two fractions with unusual antifungal activity that suppress asexual sporulation of several fungi were obtained from culture filtrate of Pseudomonas aeruginosa and were partially purified through the repeated silicagel flash column chromatographies. The sporulation-suppressive actions of these fractions in Aspergillus nidulans, Rhizopus stolonifer, and Coprinus cinereus, were analyzed by light and electron microscopes. The germination ability of the spores produced in the presence of these fractions were also checked to determine the persistent effects of these antifungal substances on the next generation. Light microscopic observation of developing sporangia of R. stolonifer grown in the presence of both fractions revealed that the significant number of sporangia failed to reach maturity, and frequently, uncontrolled growths of hyphae and rhizoids from the sporangiophores were found. In A. nidulans addition of these fractions appeared to cause different classes of morphological abnormality in conidia development, which included aborted formation of conidiogenous cells from the apex of conidiophores and enhanced hyphal growths either at the tip or middle of the conidiophores. Germination abilities of spores obtained from the cultures grown in the presence of antifungal fractions were 40∼60% in Aspergillus, 50∼80% in Coprinus (thallic spores), and 30∼40% in Rhizopus compared to those of normal spores.

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Somatic Embryogenesis and Plant Regeneration in Immature Zygotic Embryo Cultures of Hot Pepper (Capsium annuum L.) (고추의 미숙 접합배로부터 체세포배발생에 의한 식물체 재분화)

  • 정원준;민성란;유장렬;박용주;조규원
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.5
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    • pp.299-302
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    • 1994
  • Immature zygotic embryos (up to 4mm in length) were cultured on MS medium supplemented with 0.5 to 8mg/L 2,4-D. Up to 87% of them formed somatic embryos on the plumule without producing an intervening callus. The site of somatic embryo formation was confirmed by culturing plumule explants, which consisted of shoot apical meristem domes with 1 or 2 leaf primordia excised from 2-week-old seedlings. When the concentration of 2,4-D was increased over 4 mg/L, the plumule explants produced nonembryogenic calli only, whereas the distal end of the cotyledons directly formed numerous somatic embryos at frequencies of up to 60%. Upon transfer onto MS basal medium,2 out of 15 somatic embryos converted into plantlets. The plantlets were potted to a soil mixture and grown to maturity in a phytotron.

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Quality 4.0: Concept, Elements, Level Evaluation and Deployment Direction (품질 4.0: 개념, 요소, 수준 평가와 전개 방향)

  • Seo, Hojin;Byun, Jai-Hyun;Kim, Dohyun
    • Journal of Korean Society for Quality Management
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    • v.49 no.4
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    • pp.447-466
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    • 2021
  • Purpose: This article aims 1) to propose Quality 4.0 concept through surveying related literature, 2) to suggest key elements of Quality 4.0 by arranging the elements of Quality 4.0 that appeared in the literature, 3) to determine the levels of Quality 4.0, and 4) to suggest ideas for effective deployment of Quality 4.0. Methods: Eleven papers or documents are reviewed for Quality 4.0 concept; two papers and one document are investigated for key element extraction of Quality 4.0; and smart factory roadmap and industry 4.0 maturity model are studied to determine the levels of Quality 4.0. Results: 1) Quality 4.0 definition is proposed. 2) Three key elements are determined: data acquisition and analytics, connection and integration, and leadership and culture. 3) Six Quality 4.0 levels are determined. 4) Some suggestions are addressed for effective deployment of Quality 4.0. Conclusion: 1) Definition, key elements, levels, and some suggestions on effective deployment of Quality 4.0 are addressed. 2) Specific contents of Quality 4.0 education and training courses should be provided in the future. 3) Two future research directions are proposed.

High frequency Somatic Embryogenesis and Plant Regeneration in Tissue Cultures of korean Cultivar Sweet Potatoes (체세포배발생에 의한 한국 고구마 품종의 고빈도 식물체 재분화)

  • 민성란;유장렬;노태홍;김칠현;주정일
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.3
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    • pp.157-160
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    • 1994
  • Culture conditions for high Sequency somatic embryogenesis and plant regeneration in tissue cultures of sweet potato of two Korean cultivars 'Puyojaerae' and 'Yulmi' are described. Shoot apical meristem explants (height 150 $\mu\textrm{m}$; base: 350 $\mu\textrm{m}$) were cultured on MS medium supplemented with 1 mg/L 2,4-D. After 6 weeks of culture, greater than 80% of the survived explants produced embryogenic calli. When transferred onto MS medium with 0.1 mg/L each of 2,4-D and kinetin, the calli gave rise to somatic embryos at frequencies of 71% ('Puyojaerae') and 63% ('Yulmi'), respectively: When somatic embryos at various developmental stages measured in length were transversely cut into two halves and cultured on MS medium with 1 mg/L 2,L-D, the upper halves produced secondary embryos more frequently than the lower ones, and halves of somatic embryos less than 1 mm in length had a higher competence for secondary embryo formation than longer ones of either cultivar. However 'Puyojaerae' somatic embryo halves showed a higher frequency of secondary embryo formation than 'Yulmi' ones on the whole. Upon transfer onto MS basal medium, most of the primary and secondary somatic embryos underwent development into plantlets. The plantlets were transplanted to potting soil and grown to maturity in a phytotron. The overall results suggest that the shoot apical meristem culture system for somatic embryo formation in sweet potato previously established by us (SABRAOJ 21: 93-101) may be applicable regardless of it genotypes.

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Production and utilization of organic compost from spent mushroom (Pleurotus eryngii) substrate (큰느타리버섯 수확 후 배지를 이용한 유기퇴비 제조 및 활용)

  • Lee, Sang-Hwa;Park, Ju-Ri;Oh, So-Ra;Ryu, Song-Yi;Ryu, Young-Hyun;Kang, Min-Gu;Lee, Suk-Hee;Jo, Woo-Sik
    • Journal of Mushroom
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    • v.16 no.1
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    • pp.39-44
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    • 2018
  • We investigated the possibility of utilizing spent mushroom culture-medium as raw material to produce organic compost. Mushroom culture-medium contained 2.69% nitrogen, 41.07% carbon, and 0.99% phosphoric acid. Nitrogen and carbon content in rice bran were 3.08% and 47.34%, respectively. Nitrogen, carbon, and phosphoric acid content in mushroom Pleurotus eryngii were 3.41%, 34.63%, and 1.70%, respectively. Our study aimed to evaluate the degree of decay of compost produced from the substrate, used to culture King Oyster mushrooms by analyzing seed germination indexes in cucumber, radish, lettuce, and Chinese cabbage. Our results showed that compost from spent mushroom culture-medium completed the stabilization process in 4 to 6 weeks, as evaluated by the appropriate organic compost maturity point for the plantation.

Christina Rossetti's Maude : Self-Abnegation and Self-Expression of a Victorian Poet (크리스티나 로제티의 『모드』 : 빅토리아 시대 시인의 자기 단념과 자기표현)

  • Ha, Myungja
    • Cross-Cultural Studies
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    • v.25
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    • pp.391-420
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    • 2011
  • Christina Rossetti's novella Maude displays Tractarian influences in terms of Holy Eucharist, Puseyism, and the doctrine of Reserve. Tractarianism is High Church revival movement of nineteenth century. In the story a teenage girl, Maude went through hard time receiving Holy Eucharist due to self-consciousness and internal guilt according to Puseyism. She felt guilty when she enjoyed worldly things and outward beauty. Due to guilt Maude refused to receive Holy Communion, which is complete connection to God. Her cousin, Agnes suggested that in refusing Holy Communion Maude is following her own will not God's will. Later Maude overcame Puseyite thought of self-hatred and reconciled with her identity as a poet and a woman. Maude oscillates between concealing and revealing, secrecy and truth, sincerity and affectation, and modesty and display. Her marvelous poetic talent makes people praise her but she withholds private feelings and attempts to divert attention from herself. Like Maude herself, the meaning of her poems is at times reserved and withheld. This tendency goes with the doctrine of Reserve in Tractarianism. The doctrine of Reserve utilizes indirect methods to reveal divine attributes because finite human being can not accept infinite God. The doctrine of Reserve sees to it that the expression will be veiled, indirect, subdued and self-effacing. Rossetti adapts a poetic method of Reserve when Maude has anxiety over 'display and poetry' and generates the reticence, secrecy, mystery, renunciation, modesty and detachment. According to Mary Arseneau, by veiling and expressing herself through symbols she can rise above the self and employ the phenomenal to suggest a noumenal reality. Thus the poetry becomes an expression of longing for the divine. The poem "Three Nuns" exemplifies Maude's maturity and gradual progress in the relationship with God. Rossetti suggests the vision full of hopes and promises of reuniting with God. In conclusion, in some sense, authoritative and conservative Tractarianism affects Rossetti both ways. On the one hand, it makes Rossetti abnegate herself and leads her to asceticism, on the other hand, it makes Rossetti express her faith in God and write amazing devotional poems such as "Three Nuns". A poem within the poem has three voices that are in perfect harmony. In the poem the first and second nun show hesitation to fully commit to God's will and the desire for the world prevents them from having heavenly joy. Third nun reveals spiritual maturity and sings new life in God where their hopes and joys begin. Rossetti expresses the procedure of spiritual growth through the poem "Three Nuns". For Rossetti, self-abnegation and self-expression both are involved in the doctrine of Reserve, Puseyism and Holy Communion.

Effects of Development and Viability of Pig Oocytes Matured in Defined Medium Containing PVA, PVP and pFF (PVA, PVP 및 pFF를 첨가한 체외성숙 한정배지가 미성숙 돼지 난포란의 성숙과 배발달에 미치는 영향)

  • Kim I. D.;Kim S. N.;Han S. K.;Seok H. B.
    • Journal of Embryo Transfer
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    • v.19 no.3
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    • pp.219-227
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    • 2004
  • This study was conducted to develop a serum-free, defined medium of IVM of pig oocytes. The TCM-199 with supplemented with polyvinylalcohol(PVA), polyvinylpyrrollidone(PVP) and porcine follicular fluid(pFF) were used as basal medium. The effects of the these additives on the rates of maturity and development under in-vitro fertilization and in vitro culture were examined and subsequently considered on the possibilities be sustituted for the bovine serum albumin(BSA). Maturation rate of pig oocytes in IVM media containing PVA(82.4%), pFF(89.4%) and BSA(90.0%) were significantly higher(P<0.05) than that of PVP(78.6%). Cleavage rate after IVF of PVP(64%) was significantly lower(P<0.05) than these of PVA(73%), pFF(77%) and BSA(73%) supplements. in vitro development rates to morulae and blastocyst on PVP(54%) were also significantly lower(P<0.05) than these of the supplements of PVA(63%), pFF(69%) and BSA(65%). In comparison of maturation and fertilization rates of pig oocytes in each supplements, the maturity rates of PVA(82.4%), pFF(89.4%) and BSA(90.0%) were significantly lower(P<0.05) than that of PVP(72.4%) and while, the fertilization rates of pFF(87.1%) and BSA(89.1%) were significantly higher(P<0.05) than these of PVA(78.0%) and PVP(70.6%). It may be concluded that PVA and pFF can be substituted far BSA in medium for culturing pig oocytes; however, it may be considered that PVP were limited to for BSA in the in vitro culture of the embryos.

High Quality and High Yielding Rice Variety 'Cheongdam' Adaptable to Direct Seeding (고품질 다수성 직파재배적성 신품종 '청담벼')

  • Choi, Im-Soo;Kang, Kyung-Ho;Jeong, O-Young;Jeong, Eung-Gi;Cho, Young-Chan;Kim, Yeon-Gyu;Kim, Myeong-Ki;O, Myeong-Gyu;Choi, In-Bea;Jeon, Yong-Hee;Won, Young-Jae;Shin, Young-Seoup;Oh, In-seok
    • Korean Journal of Breeding Science
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    • v.43 no.6
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    • pp.581-586
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    • 2011
  • 'Cheongdam' is a japonica rice variety developed from a cross between SR19200-HB826-34, a line of good germination ability and shoot emergence at low temperature and Juanbyeo, good quality and direct-seeding adaptable cultivar by the rice breeding team of National Institute of Crop Science, RDA in 2006. This variety has 153 days of total growth duration from seeding to maturity in direct-seeding, and 160 days of growth duration from seeding to maturity in transplanting. This is erect plant type with culm length of 74 cm, thick culm, and green leaves. It has large panicle shape with 126 and 140 spikelets per panicle in direct-seeding and transplanting, respectively. Milled rice is transluscent and medium in grain size of non-glutinous endosperm. This variety is susceptible to leaf and neck blast, bacterial blight, stripe virus disease and brown planthopper. The yield potential of 'Cheongdam' is 5.84 MT/ha at ordinary transplanting culture and 5.62 MT/ha and 5.89 MT/ha at wet direct-seeding and dry direct-seeding cultures, respectively in the local adaptability test for three years. 'Cheongdam' would be adaptable to middle and southern plain of Korea for direct-seeding culture and transplanting rice culture.