• Journal of Plant Biotechnology
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• 제36권2호
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• pp.193-196
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• 2009

Pinellia koreana K-H Tae & J-H Kim is a recently discovered Korea endemic medicinal plant species whose natural habitat is rapidly destroyed by industrial development. Described in this paper are culture conditions for high frequency plant regeneration via bulblet formation from leaf explant cultures of P. koreana. Leaf explants formed white nodular structures and off-white calluses at a frequency of 91.2% when cultured on MS medium supplemented with 2 mg/L BA and 0.5 mg/L NAA. However, the frequency of white nodular structures and off-white calluses formation was slightly decreased with an increasing concentration of NAA up to 4 mg/L, where the frequency reached 31.7%. Most petiole explants did not form white nodular structures and off-white calluses except the combination treatment of 2 mg/L BA and 2 mg/L NAA. Upon transfer onto MS basal medium, over 90% of nodular structures gave rise to numerous bulblets and developed into plantlets. Plantlets regenerated from bulblets were transplanted to potting soil and grown to maturity at a survival rate of over 95% in a growth chamber. Therefore, the in vitro plant regeneration system of P. koreana obtained in this study will be useful for mass propagation and long-term preservation of genetic resources of P. koreana.

• 성인간호학회지
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• 제20권6호
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• pp.852-865
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• 2008

Purpose: This study was designed to explore what experiences nurses had while caring and providing emotional support for patients. Methods: Participants were eight nurses working at hospitals for more than one year. Data were collected from June, 2006 to January, 2007 through in-depth interview by using tape-recordings. Data were analysed with the phenomenological method proposed by Colazzi(1978). Results: From significant statements, 4 clustered themes, 7 themes and 23 sub-themes were extracted from the essential meaning of the emotional experience of hospital nurses. The 4 clustered themes were 'movement of mind', 'affection and service for patients', 'worthwhile and conflict' and 'control oneself'. The 7 themes were 'special feeling', 'rapport formation', 'consideration', 'human interaction', 'value discovery', 'loss of volition', and 'keep to balance'. Conclusion: Although nurses had tough experiences for providing care for patients' emotional support, they had also experienced spiritual maturity from its experience. The result of this study would contribute for nurses not only to care for patients who need emotional support but also to develop knowledge in nursing.

• 한국의류산업학회지
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• 제19권4호
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• pp.365-372
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• 2017

This study systematically identified the influence of the school look fashion image on public culture, which is used for increasingly sexualized marketing appeal by domestic Girl Groups. We examined and analyzed the school look fashion image of Girl Groups, focusing on the Lolita complex which is particularly influential in the sexual appeal of domestic popular fashion. The method of this study is based on a literature review from the years 2007 to 2016, when the female girl groups began to receive attention. The music videos of the female girl groups in the top 100 charts of 'Melon' from 2006 to 2-16, which provides the largest mobile music service in Korea, were watched and analyzed as primary data. As a result, it was found that the 'school fashion look image' of adolescence which was used as costumes for Girl Groups, plays a role in commercializing the image of a 'girl', and the types and characteristics of school look fashion image are drawn in two ways. First, it is the image of a seductive Lolita complex. This is the case where young girls wear school look fashion image to emphasize their sexual maturity. Second, it is the case that is using the school look fashion image in order to perform with the 'young girl' concept, as an image of the enchanting Lolita complex; in addition, the erotic body image is more explicitly exposed through choreography and nakedness.

• 한국작물학회지
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• 제44권2호
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• pp.143-148
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• 1999

Soil moisture condition is an important limiting factor in growth and yield in rice culture. The purpose of this study was to compare the influence on the growth, yield and Quality of rice subjected to soil moisture stress (SMS) at different growth stages. Ajaponica rice cultivar, Dongjinbyeo, was cultured under flooded conditions in a plastic container filled with silty loam soil. The container was subjected to SMS until the initial wilting point (IWP) coincided with about 10% in soil moisture content and about-200 kPa in soil matric potential, and was then irrigated again, at 6 and 5 of main growth stage in 1996 and 1997, respectively. At maturity, the plant height, tiller number, leaf area and top dry weight were decreased more in SMS treatments at the early stage than the late stage. The averaged yield index of SMS to control in both years was lowest at meiosis (62.5%), which primarily resulted from lower percent ripened grain and 1,000 grain weight, and second＇ reduced the spikelet number per panicle and panicle number per hill, and followed at tillering stage (68.5%) which resulted from the lower production in tiller number and top dry matter during and after SMS treatment. The percent-age of read rice in SMS plants varied with the treatment stage as order of lower at meiosis (44.0%), heading (53.9%), panicle initiation (70.1%), tillering (72.1%), ripening (75.8%) and 5 days after transplanting (DAT) (79.0%). Protein content in brown rice was slightly larger in SMS at late growth stage than the control, while the contents of fat and ash differed very little between SMS and control. Contents of Mg and K and Mg/K in brown rice with SMS were lower at some treatment stages such as at ripening or panicle initiation.

• 식물조직배양학회지
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• 제26권4호
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• pp.289-291
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• 1999

발아 7일된 오이 (Cucumis sativus L.) F$_1$ 5품종과 순계 4품종의 하배축 절편을 1mg/L 2,4-D가 첨가된 MS고체배지에 치상하여 3주간 배양하였다. F$_1$ 1품종과 순계 2품종에서 발달한 캘러스의 8%이하에서 체세포배가 발생하였다. 체세포 배 절편을 동일한 배지에 4주간 배양하여 배발생캘러스를 유도하였다. 배발생캘러스는 동일조성의 MS액체배지에 현탁되어 증식하였다. 액체배지에서 배발생캘러스는 수많은 체세포 배로 발달하였으며, 생장조절제가 첨가되지 않은 MS기본배지에서 95%이상의 체세포배가 식물체로 발아하였다. 재분화된 식물체는 화분에서 생육되어 개화 후 착과하였다.

• Journal of Microbiology
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• 제44권3호
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• pp.363-368
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• 2006

In this study, native Byadyrhizobium strains were isolated from the host plant, Glycine max, harvested from fields in Madhya Pradesh, India, and were typed by Iytic rhizobiophages. Eight indigenous (Soy2, ASR011, ASR031, ASR032, MSR091, ISR050, ISR076 and ISR078) and two exotic strains (USDA123 and CB1809), all of which evidenced a distinct reaction with six phages, were employed in this study. The symbiotic interaction of these strains was studied initially using soybean cultivar JS335 in a sand culture in a controlled environment, and the efficiency was assessed based on the nodule number, nodule dry weight, plant dry weight, nitrogenase activity, and total accumulation of N per plant. Symbiotic effectiveness was found to be highest with the native phage-sensitive isolate ASR011, whereas it was at a minimum with the phage-resistant isolates, ISR050 and ISR078. Additionally, the effectiveness of these strains was evaluated using six soybean cultivars belonging to different maturity groups; namely, Brags, Lee, Pusa20, PK416, JS33S and NRC37. Analysis of variance data evidenced significant differences due to both symbionts, for the majority of the tested parameters. The CB1809, USDA123, and ASR011 strains evidenced relatively superior symbiotic effectiveness with soybean cultivars Brags, Lee and JS335. Strain ISR078 evidenced no significant responses with any of the cultivars. The ASR031 strain performed moderately well with all tested cultivars. The symbiotic response of all the strains was quite poor with cultivar PK416. Our studies showed that a significant relationship existed between the phage sensitivity and symbiotic efficiency of the bacterial strains with the host-cultivars.

• 한국경제지리학회지
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• 제8권2호
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• pp.301-314
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• 2005

본 연구의 목적은 일본 국민보양온천 관광지역에 있어 관광객의 일반적 특성과 다양한 관광자원에 대한 평가를 통하여 쇠퇴하고 있는 지역관광의 특성화된 개발전략을 고찰하는데 있다. 버블경제 붕괴이후 성숙기에 접어든 오늘날 일본 국내관광은 지역 관광산업의 질적 변화를 요구하고 있다. 온천 개발의 역사가 길고, 문화자원이 풍부한 카케유지역은 `보양과 요양` 테마를 강화한 리허빌리테이션 기능으로 특성화되고 있다. 노년층의 여성 관광객 비중이 높은 카케유온천은 건강회복이나 병 치료를 목적으로 방문하기 때문에 체류기간이 비교적 길고, 대중교통을 이용하는 단골고객이 많다. 관광자원에 대한 평가는 숙박시설에서 가깝고, 편안한 산책로나 엄숙한 사찰경관에 대한 관심이 높게 나타났다. 이러한 수요 특성을 반영하기 위하여 지역사회는 풍부한 온천 $\cdot$ 문화 $\cdot$ 자연을 토대로 리허빌리테이션 강화, 건강음식 개발, 자원봉사활동 강화, 배리어프리 사업 등을 적극적으로 전개해 나가고 있다.

• 농업과학연구
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• 제37권2호
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• pp.303-308
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• 2010

Surface color is the most important quality factor for the grade evaluation of cherry tomato. Color is one of the representative indicators for the maturity which is closely related to the internal quality of cherry tomato, such as firmness, sugar content, and acidity. This study was carried out to investigate the relationship between surface color and internal quality of cherry tomatoes harvested from both hydroponic and soil culture at different ripening stages. To calculate the color values of cherry tomatoes an automatic color imaging system was constructed. A specially designed image processing algorithm for the color measurement was developed. The color values of L*, a*, b* were calculated from the initial color values of RGB and then compared with the internal quality. Statistical analyses indicated that the internal quality was more highly correlated with the surface color than size of cherry tomatoes. Color image features were also investigated to detect external damage of cherry tomatoes. The value of (R value - R mean value)/R mean value was the most effective image feature for the detection of damaged areas on the surface of cherry tomatoes. The results of this study demonstrated the feasibility of color sorting process as an alternative of the conventional drum type size sorting system for cherry tomato industry.

• 한국수정란이식학회지
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• 제30권3호
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• pp.213-218
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• 2015

In the study for a differentiation and development of spermatogonial cells, the researchers should commonly require a simple, fast and reasonable method that could evaluate the developmental stage of male germ cells without any damage and also relentlessly culture them so far as a cell stage aiming at experimental applications. For developing the efficient method to identify the stage of sperm cells, the morphological characteristics of sperm cells were investigated by staining the cells with blue fluorescent dye Hoechst 33258, and a criterion for male germ cell classification was elicited from results of the previous investigation, then the efficiency of the criterion was verified by applying it to assort the germ cells recovered from male mice in age from 6 to 35 days. As morphological characteristics, spermatogonia significantly differed from spermatocytes in size, appearance and fluorescent patches of nucleus, and spermatids could also be distinguished from spermatozoa by making a difference in the volume and shape of nucleus and the shape and fluorescence of tail. Aforesaid criterion was applicable for classifying in vitro cultured sperm cells by verifying its efficiency and propriety for assorting the stages of testicular germ cells. However, the fluorescent staining showed that germ cells in mouse testis should be dramatically differentiated and developed at 21 days and 35 days of age, which were known as times of sexual puberty and maturity in male mice, respectively. In conclusion, the results indicated that this simple criterion for sperm cell classification using fluorescence staining with Hoechst 33258 may be highly efficient and reasonable for spermatogenesis study.

• 한국작물학회지
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• 제21권1호
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• pp.1-8
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• 1976

논, 밭 양조건하에서 수륙도를 재배하여 생육과 수량을 비교하였다. 수도는 영남조생과 유신을, 육도는 하다상고구와 다찌미노리를 공시하했으며 처리내용은 무관개표준비, 관개표준비, 관개질소증비의 3구는 밭에, 관수표준비구는 논에 설치하여 1975년에 시험을 실시하였던 바 그 결과를 요약하면 다음과 같다. 1. 수도는 논조건하에서 현미 수량이 많았고 유신은 $10^{a}$ 당 431.7kg으로서 가장 많았으며 육도는 논보다 밭에서 증수하였다. 2. 밭조건하에서 질소 증비에 의한 증수는 수도의 영남조생과 유신은 인정되나, 육도 2품종은 증수를 인정할 수 없었다. 3. 영남조생과 유신의 출수기는 논조건하에서 보다 밭조건하에서 약 1주일 늦었다. 4. 고중은 수도는 논, 밭조건하에서 차이가 없었으나, 육도는 논보다 밭에서 많았다. 5. 10cm간 수수는 논ㆍ밭 어느 조건하에서도 수도가 많고 육도는 논, 밭조건하에 의한 차이를 인정할수 없었다. 6. 등숙비율과 천립중은 육도가 수도보다 논, 밭 어느 조건하에서도 컸으며 논과 밭의 비교에서는 수륙도 모두 논조건하에서 컸다. 7. 수도는 논에서, 육도는 밭에서 일수립수가 많은 경향을 보였다. 8. 엽신의 질소함량은 전생육기간을 통하여 공히 논조건하에서 보다 밭조건하에서 많았다.