• KSBB Journal
• /
• v.5 no.3
• /
• pp.269-277
• /
• 1990

Methylobacillus sp. SK1, an obligate methylotroph, was cultivated in a fed-batch culture using DO as a methanol feeding indicator with a micro computer-aided control system. While 2.07g/l of cell density was obtained after 13 hr in the batch culture (initial methanol concentration: 1.0%(v/v)),45.3g/l of cell density was obtained after 17 hr by feeding methanol and metal ions in the fed-batch culture with oxygen supply. The high-density biomass was obtained in short cultuivation time by fed-batch culture with feedback control, and consequently the biomass productivity was significantly increased. It was mainly due to extension of logarithmic growth period by methanol feeding without methanol inhibition and intensive aeration without DO limitation with microcomputer-aided control system.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.36 no.6
• /
• pp.606-613
• /
• 2003

This study investigated the effects of food type (condensed freshwater Chlorella, dried Chlorella, dried Spirulina, dried Schizochytrium, baker's yeast and $\omega-yeast$) and amount, and supplementation of vitamin $B_{12}$ on the growth of freshwater rotifer (Brachionus calyciflorus) in high density culture. Growth of rotifers fed condensed freshwater Chlorella was the highest and its density ranged $7.65-8.14{\times}10^3\;inds./mL.$ The primary lipid acids of rotifers fed condensed freshwater Chloyella were linoleic and linolenic, and their amount ($\%$ of total fatty acids) were $48.8\%\;and\;26.8\%,$ respectively. This suggests that condensed freshwater Chlorella would be an effective diet for high quality and quantity rotifers, which in turn serve as live food for freshwater fish larvae. Growth rate of rotifers with Chlorella supplementation increased as amount of supplementation increased up to 1.5 and 2.5 mg at 28 and $32^{\circ}C$, respectively. However, undissolved ammonia toxicity and packing volume of Chlorella in culture medium, reached the optimal conditions for the stable and effective cultivation of rotifers when amount of condensed freshwater Chlorella was 1.5 mg in dry weight per 1,000 rotifers at $28^{\circ}C\;and\;32^{\circ}C$ Growth of rotifers in condensed freshwater Chlorella with vitamin $B_{12}$ supplementation was significantly higher than that of rotifers without supplementation. However, no significant difference was found among the different concentrations of vitamin $B_{12}.$ Therefore, vitamin $B_{12}$ could improve the growth of rotifers (B. calyciflorus).

• Journal of the Korean Society of Food Culture
• /
• v.21 no.6
• /
• pp.596-605
• /
• 2006

This study was conducted to investigate factors affecting gone density of university students in Seoul area. Data for food habits, exercise and health-related behaviors were obtained by self administered questionnaires. BQI(bone quality index) of the subjects was measured by an Quantitative Ultrasound(QUS). The results are summarized as follows: The average hight, weight BMI and osteopenia percentage of the male and female student were 173.3cm, 68.6kg, 22.7 and 24.2%; 161.4cm, 54.4kg, 20.9 and 55.5%, respectively. The BQI and Z-score of the subjects were 99.6, -0.3 in male student group, and 82.7, -1.1 in female student group, respectively. Height, weight, fat weight, fat mass and BMI were positively related with BQI in female group. BQI was positively affected by breakfast and frequence exercise in male student group. In female student group, frequency exercise was positively related with BQI. The result of this study revealed that the desirable food habits, dietary behaviors and health-related lifestyles may have a beneficial effect on bone density. They should have practically and systematically organized nutritional education on optimum body weight, good eating habits, weight bearing exercise for higher bone density level.

• Journal of Microbiology and Biotechnology
• /
• v.10 no.4
• /
• pp.470-475
• /
• 2000

Genetic chracterstics of the structural gene of guamerin (a novel elastase inhibitor from Korean leech), integrated into the HIS4 locus of chromosomal DNA of Pichia pastoris along with the $\alpha$-factor leader sequence, were investigated. In the selected clone from candidates, two copies of the integration cassette including the structural gene copies of the integration cassette including the structural gene of guamerin were found in the integration site of the chromosomal DNA of P.pastoris. It was demonstrated that the integrated structural gene of guamerin was stable up to about 70 generations in the relay flask culture. Then, a high-cell-density culture could be fulfilled easily by DO-stat fed-batch culture, in which the cell growth and the recombinant guamerin production reached about 250 of OD600nm and 260 mg/l, respectively. Finally, it was revealed that the DNA sequence of the integrated structural gene of guamerin in P. pastoris was maintained correctly in the end of production cells of relay flask culture and high-cell-density culture.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.40 no.1
• /
• pp.39-43
• /
• 1995

In the culture of Vinyl mulching and Non mulching, growth, yield, and cotton hair's growth are studied at the different planting density to get a basic material for cotton culture and high yield bumper crop in the region of the South. And the results are following. Vinyl mulching is the best one at the strength, the number of boll bearing branches, the num-ber of bolls, the number of bolls per $m^2$ and all the other characters. Vinyl mulching and Non mulching have the greatest number of bolls per $m^2$ at the planting density of 70${\times}$10cm. Vinyl mulching has the highest seed cotton yield and the highest seed cotton yield per boll. Especially when planted at the density of 70${\times}$l0cm, Vinyl mulching and Non mulching have the highest seed cotton yield. Vinyl mulching's value is accepted in fiber length and lint percentage, the wider spacing in the row is, the longer fiber length is.

• 한국생물공학회:학술대회논문집
• /
• 2001.11a
• /
• pp.845-848
• /
• 2001

For understanding physiology and metabolism under various culture conditions, combined analysis of transcriptome and proteome is attractable way. We have manufactured DNA microarray containing 2,850 genes including all functionally known and putative ones. In this study, we report analysis of transcriptome and proteome during the high cell density culture of E. coli by using DNA microarray and 2-DE. Fed-batch fermentation of E. coli was carried out by exponential feeding of nutrients until the maximum cell density reached 74 g dry cell weight/L (g DCW/L). Changes in transcriptome and proteome during the HCDC are analyzed qualitatively and quantitatively to provide their physiological and metabolic meanings.

• International Journal of Advanced Culture Technology
• /
• v.9 no.2
• /
• pp.32-38
• /
• 2021

The purpose of this study is to examine the spatial characteristics of violent and burglary crimes in South Korea. Violent crimes and burglary crimes depend on a spatial setting with good conditions for their criminal purposes. This study defines population density, racial heterogeneity, types of houses, and density of commercial facilities as variables of place affecting crime in cities and counties. The study collects data from 229 cities in Korea to analyze the effect of spatial characteristics on crime. We conduct additional analyses to meet the statistical requisites of the spatial econometrics model using the open-source software R and GeoDa 1.12.1.129. From the analytical result, population density, racial heterogeneity, apartments, and commercial areas relate to crime occurrence. We suggest the implication of the theoretical and practical contributions to the relationship between place and crime.

• Korean Journal of Medicinal Crop Science
• /
• v.26 no.1
• /
• pp.64-71
• /
• 2018

Background: The cultivation of ginseng (Panax ginseng C. A. Meyer) in greenhouses could reduce the use of pesticides and result in higher yield; however, construction costs are problematic. The adaptation of direct-sowing culture in greenhouses could reduce the cost of ginseng production. Methods and Results: To improve seedling establishment in direct-sowing culture, effects of sowing density (SD), number of seeds sown per hole (SN), and thinning (TH) treatment on the root yield were investigated after 3 years of seeding. The emergence rate was significantly influenced by SD, but not by SN or TH. Damping-off and rusty roots increased with an increase in SN with diminishing effects of SN on seedling establishment. Root weight and diameter were affected by SD, SN, and TH, however, there were no statistical significances. The total number of roots harvested per unit area increased with increasing SD and SN, and the weight of roots was affected by SD, but not by SN or TH. Conclusions: Multi-seed sowing per hole and/or thinning might not be an efficient method for the direct-sowing culture of ginseng. The SD for direct seeding culture in greenhouses should be approximately $33-42seeds/m^2$ for an optimum yield of 3-year-old ginseng.

• Microbiology and Biotechnology Letters
• /
• v.30 no.3
• /
• pp.264-269
• /
• 2002

The production of exopolysaccharide from Zoogloea ramigera 115SLR in batch culture was affected by carbon sources, rifampicin concentration, inoculum size and cell density of starter culture. The increase of organic nitrogen concentration and cell density in defined medium by adding starter culture resulted in higher production of exopolysaccharide (EPS) within 2 days. Glucose and lactose as carbon sources showed EPS production of 10.7 gk and 10.5 ga, respectively. Higher EPS production was obtained with 2.5% (w/v) glucose. White sugar, brown sugar and galactose produced less than 10 gt of EPS. The optical density of starter culture affected on EPS yield, producing over 10 gt of EPS in the range of 1.0-1.7. In the presence of rifsmpicin, Z. ramigera 115SLR produced EPS of 12.9 gt. Molecular weight of EPS produced with/without rifampicin was determined with 1.367$\times$106 and 1.711$\times$106 g/mol using HPSEC-MALLS-RI, respectively.

• KSBB Journal
• /
• v.6 no.3
• /
• pp.231-240
• /
• 1991

Recently, developments of large scale and high density cell culture methods have been the objects of many researches, because the demand of various pharmaceutical products produced by animal cell culture has been rapidly increasing. The cell culture equipment should have the requirements such as sufficient oxygen transfer and mixing, low shear stress and surface tension, and small foaming. In order to develop a proper bioreactor meeting these requirements simultaneously, a perfluorocarbon having high solubility of oxygen was sprayed into the medium as an oxygen carrier instead of air. Also, a new impeller was developed and combined together with the perfluorocarbon spraying system so as to design a new bioreartor for cell cultivation. The new impeller had better characteristics of mixing and oxygen transfer than the paddle and cell-lift impellers based on the same, shear rate. But, it was observed that the volumetric oxygen transfer coefficient of the new bioreactor decreased with increasing cell density during E. coli fermentation.