• Title/Summary/Keyword: Culture Technology Institute

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Production of Shikonin by A Hairy Root Culture of Lithospermum erythrorhizon

  • Seo, Weon-Taek;Park, Young-Hoon;Choe, Tae-Boo
    • Journal of Microbiology and Biotechnology
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    • v.2 no.1
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    • pp.41-45
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    • 1992
  • Shikonin production was examined in a bubble column bioreactor system with the hairy roots of Lithosphermum erythrorhizon. The volumetric productivity was higher than those obtained from other reactor configurations with free or immobilized cells of the same cell line. The productivities of the bubble column reactor, with and without a product absorption trap, were 7.4 and 4.5 mg of shikonin/l/d, respectively. This indicated the importance of the product removal in the design and operation of the shikonin production system with hairy root culture.

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Production of Red Pigments by Monascus purpureus in Submerged Culture

  • Lee, Bum-Kyu;Park, No-Hwan;Piao, Hai-Yon;Chung, Wook-Jin
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.5
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    • pp.341-346
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    • 2001
  • For the purpose of mass producing Monascus red pigments optimum medium composition and environmental conditions were investigated in submerged flask cultures. The optimum carbon and nitrogen sources were determined to be 30g/L of glucose and 1.5 g/L of monosodium glutamate (MSG). Of the three metals examined, Fe$\^$2+/ showed the strongest stimulatory effect on pigment production and some stimulatory effect was also found in Mn$\^$2+/. Optimum pH and agitation speed were determined to be 6.5 and 700 rpm, respectively. Under the optimum culture conditions batch fermentation showed that the maximum biomass yield and specific productivity of red pigments were 0.20 g DCW/g glucose and, 32.5 OD$\sub$500/g DCW$\^$-1/h$\^$-1/, respectively.

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Optimization of Food Waste Fermentation for Probiotic Feed Production with Yeast Kluyveromyces marxianus

  • Lee, Ki-Young;Yu, Sung-Jin;Yu, Seung-Yeng
    • Proceedings of the Korean Institute of Resources Recycling Conference
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    • 2001.05b
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    • pp.121-125
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    • 2001
  • For the probiotic feed production, aerobic liquid fermentation of pulverized food wastes was attempted with a yeast Kluyveromyces marxianus. After grinding finely, optimal fermentation conditions of the substrate was investigated by shaking culture. The most active growth of the yeast was shown at solid content of 10%. The proper addition of urea(0.5g/l), o-phosphate(0.4g/l), molasses(4g/l), and yeast extract (1g/1) increased cell growth rate and viable cell count. For optimizing, the nutrients were all added to substrate and fermentation was carried in 2 litre jar fermenter. For the stimulation of hydrolyzing enzyme excretion, mixed culture with Aspersillus oryzae was also conducted. In 12 hours of fermentation, viable cell count of the yeast Kluyveromyces marxianus amounted to the number of 1.4 $\times$10$^{10}$ /1 in the culture medium.

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Traditional Culture's Cloud Digital Archive by approach to the Convergence (융복합 접근을 통한 고전문학 클라우드 디지털 아카이브)

  • Kim, Dong-Gun;Jeong, Hwa-Young
    • Journal of Advanced Navigation Technology
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    • v.16 no.1
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    • pp.116-121
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    • 2012
  • This research aimed an application method to connect traditional culture and information technology that is one of the digital convergence area. For this purpose, we proposed cloud digital archive framework for traditional culture. Actually, cloud computing environment is one of new technology trend and would be a important keyword for information technology. This research focus on functional framework that is able to support service in cloud computing environment to connect pansori archive model, one of traditional culture. We also make a data structure and metadata by each characteristics, and insert a service framework to support pansori archive. This structure consists of data evaluation, preservation, and access process. This archive has a relation model with digital archive and metadata.

In Vitro Mass Propagation and Economic Effects of Bioreactor Culture in Ever-bearing Strawberry 'Goha' (Bioreactor를 이용한 사계성 딸기 기내대량증식과 경제성)

  • Lee, Jong-Nam;Kim, Hye-Jin;Kim, Ki-Deog;Kwon, Young-Seok;Im, Ju-Sung;Lim, Hak-Tae;Yeoung, Young-Rok
    • Horticultural Science & Technology
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    • v.28 no.5
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    • pp.845-849
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    • 2010
  • This study was conducted to compare various culture methods and evaluate economic feasibility of each method for mass propagation of new ever-bearing strawberry 'Goha'. Four different methods such as semi-solid culture, solid culture, liquid suspension culture and bioreactor culture were compared. The solid culture and bioreactor culture showed the shortest and longest root length, such as 3.6 cm and 8.3 cm, respectively. Fresh weights of plants cultured in bioreactor were 2,261 mg, which were heavier than those of cultures. Dry weights of plants cultured in bioreactor were the heavier compared to those in other cultures. The number of axillary bud developed in bioreactor was seven, but axillary bud was not developed in other cultures. Production cost through bioreactor culture was calculated to be 303 won per plant which was 542 won less than that of solid culture. As a result, we found that the bioreactor culture was the most cost effective culture method for in vitro mass propagation in new ever-bearing strawberry 'Goha'.

A Study on Classification of Wood Cultural Resources in South Korea (목재문화자원의 유형 분류에 관한 연구)

  • HAN, Yeonjung;LEE, Sang-Min;CHOI, Jinyoung;PARK, Chun-Young
    • Journal of the Korean Wood Science and Technology
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    • v.49 no.5
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    • pp.430-452
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    • 2021
  • The recent social atmosphere has been a preference for wood utilization and woodworks. The general public does not have many opportunities to enjoy wood culture, so there is a lack of awareness and foundation of wood culture. In this study, classification and case analysis of wood culture were conducted as basic research for establishing a promotion strategy for the general public to enjoy wood culture. The specificity of wood culture and cultural resources was analyzed to establish the concept of wood cultural resources. Through the analysis, wood cultural resources were defined as products created as a result of human activities that implied the cultural value of wood and wood use in terms of conservation, discovery, and utilization. The types of wood cultural resources were classified into seven categories using the classification examples performed on cultural resources: cultural heritage, cultural facilities, cultural festival, wood architecture, culture contents, culture education, and wood products. In addition, cases were searched and proposed for each type of wood cultural resources.

Inspirations for China's Cultural Industry Development from the Construction of Korea's Cultural Industry Chain

  • Guo, Pingjian;Fang, Haixia
    • The International Journal of Costume Culture
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    • v.13 no.2
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    • pp.88-92
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    • 2010
  • The purpose of this research was to understand the successful establishment of the cultural industry chain in Korea and discover lessons for China to improve its cultural industry. It was concluded that a one-industry development pattern cannot win in market competition and a cultural industry will strengthen its sustainability only through smoothing its relationship with other industries and establishing a cultural industry chain so as to further development and resist crises together.

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Isolation and Characterization of Antimicrobial Substance Macrolactin A Produced from Bacillus amyloliquefaciens CHO104 Isolated from Soil

  • Lee, Seung-Je;Cho, Jeong-Yong;Cho, Jung-Il;Moon, Jae-Hak;Park, Ki-Deok;Lee, Young-Ju;Park, Keun-Hyung
    • Journal of Microbiology and Biotechnology
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    • v.14 no.3
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    • pp.525-531
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    • 2004
  • A strain antagonistic to Fusarium solani, CHO104, was selected from approximately 100 microorganisms isolated from soil. Strain CHO104 was identified as Bacillus amyloliquefaciens and found to be Gram-positive based on the Biolog system and 16S rRNA methods. A culture broth of B. amyloliquefaciens CHO104 also exhibited antimicrobial activity against various microorganisms. As such, the EtOAc extract of the culture broth was isolated by various column chromatographic procedures and HPLC. The antimicrobial and antifungal substance was then characterized as macrolactin A $(C_{24}H_{34}O_5)$ using high-resolution EI-MS and NMR analyses, and found to be very effective in inhibiting the growth of Staphylococcus aureus, E. coli, and Botrytis cinerea, even when using a concentration of one-twentieth of the benzoic acid as the control compound.