• Title/Summary/Keyword: Culture Technology Institute

Search Result 2,450, Processing Time 0.035 seconds

Evaluation of Nutrients during Rack and Bag Culture or Suspended Culture of Pacific Oyster Crassostrea gigas (부유망식과 수하식 양성방법에 따른 참굴(Crassostrea gigas)의 영양상태)

  • Choi, Yong-Jun;Tri, Nguyen Thanh;Lee, Jeong-Mee;Kang, Seok-Joong;Choi, Byeong-Dae
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.50 no.3
    • /
    • pp.263-269
    • /
    • 2017
  • The mineral contents, and free amino acid, and fatty acid composition of rack and bag or suspended cultured oysters Crassostrea gigas were compared. The moisture content did not change much from 82.8% to 86.3% in the different culture methods. The glycogen level was low 4.7% during rack and bag culture but high 7.2% during suspended culture. The sodium level was the highest during suspended culture, while copper and zinc level were highest during rack and bag culture. The taurine level was 1,385-1,375 mg/100 g in oyster cultured using the rack and bag method, but that in the market sample was lowest at 1,015.8 mg/100 g. Hydroxyproline, glutamic acid, proline, glycine, and alanine were detected at high levels in the experimental oysters. The polyunsaturated fatty acid content was similar among samples. The docosahexaenoic acid level was 14% with rack and bag culture and 12% with suspended culture, whereas the eicosapentaenoic acid level was 15% with rack and bag culture and 20% with suspended culture. The atherogenic index (AI), thrombogenicity index (TI), and hypocholesterolemic/ hypercholesterolemic (h/H) ratio were important factors in the nutritional evaluation. The AI and TI values were 0.5-0.2, and the h/H ratio were 2.0 and 2.4 with the rack and bag and suspended culture, respectively.

Mass Production of Paclitaxel by Plant Cell Culture (식물세포배양에 의한 항암제 Paclitaxel의 대량 생산)

  • Choi, Hyung-Kyoon;Son, Joo-Sun;Na, Gwang-Hwee;Hong, Seung-Suh;Park, Yeon-Seung;Song, Jai-Young
    • Journal of Plant Biotechnology
    • /
    • v.29 no.1
    • /
    • pp.59-62
    • /
    • 2002
  • Samyang Genex succeeded in commercialization of anticancer agent-paclitaxel by plant cell culture technology. The core technology of Samyang Genex relating paclitaxel production includes cell line development, cell line preservation, cell culture, scale-up technology, and purification technology. On the basis of the research, Samyang Genex built the factory operated by CGMP (current good manufacturing practice). The paclitaxel-Genexol$^{TM}$-is commercially available in Korea, and it will be launched to world market including USA after approval of US FDA.

Biodegradation of 2,4,5-Trichlorophenol Using Cell-Free Culture Broths of Phanerochaete chrysosporium

  • Choi, Sueh-Yung;Moon, Seung-Hyeon;Lee, Jae-Suk;Gu, Man-Bock
    • Journal of Microbiology and Biotechnology
    • /
    • v.10 no.6
    • /
    • pp.759-763
    • /
    • 2000
  • Cell-free culture broth of Phanerochaete chrysosporium has been adopted to biologically degrade 2,4,5-trichlorophenol. Two different medium compositions of nitrogen-sufficient and nitrogen-limited were compared for their distribution of isozymes, activity of lignin peroxidase, and production of oxalate. The two different culture broths were tested for their ability to degrade 2,4,5-trichlorophenol, and the biodegradation efficiency was estimated in terms of the disappearance of 2,4,5-trichlorophenol. The degradation efficiency for the nitrogen-limited culture broth was higher than that of the nitrogen-sufficient culture broth, since the nitrogen-limited culture broth induced lignin peroxidases (LiPs) and manganese peroxidases (MnPs), and contained sufficient oxalate for producing necessary radicals. Finally, the possible mechanism of 2,4,5-CP degradation using the nitrogen-limited culture broth was proposed.

  • PDF

Mass Production of Paclitaxel by Plant Cell Culture (식물세포배양에 의한 항암제 Paclitaxel의 대랑 생산)

  • CHOI Hyung-Kyoon;SON Joo-Sun;NA Gwang-Hwee;HONG Seung-Suh;SONG Jai-Young
    • Proceedings of the Korean Society of Plant Biotechnology Conference
    • /
    • 2002.04a
    • /
    • pp.27-31
    • /
    • 2002
  • Samyang Genex succeeded in commercialization of anticancer agent-paclitaxel by plant cell culture technology. The core technology of Samyang Genex relating paclitaxel production includes cell line development, cell line preservation, cell culture, scale-up technology, and purification technology. On the basis of the research, Samyang Genex built the factory operated by CGMP (current good manufacturing practice). The $paclitaxel-Genexol^{TM}$ is commercially available in Korea, and it will be launched to world market including USA after approval US FDA.

  • PDF

Mass Production of Paclitaxel by Plant Cell Culture (식물세포배양에 의한 항암제 Paclitaxel의 대량 생산)

  • Choi, Hyung-Kyoon;Son, Joo-Sun;Na, Gwang-Hee;Hong, Seung-Suh;Song, Jai-Young
    • Proceedings of the Korean Society of Plant Biotechnology Conference
    • /
    • 2002.04b
    • /
    • pp.27-31
    • /
    • 2002
  • Samyang Genex succeeded in commercialization of anticancer agent-paclitaxel by plant cell culture technology. The core technology of Samyang Genex relating paclitaxel production includes cell line development, cell line preservation, cell culture, scale-up technology, and purification technology. On the basis of the research, Samyang Genex built the factory operated by CGMP (current good manufacturing practice). The $paclitaxel-Cenexol^{TH}-is$ commercially available in Korea, and it will be launched to world market including USA after approval of US FDA.

  • PDF

Production of Gericudranins by Hairy Root Culture of Cudrania tricuspidata

  • Seo, Weon-Taek;Lee, In-Kyoung;Yoo, Ick-Dong;Park , Young-Hoon
    • Journal of Microbiology and Biotechnology
    • /
    • v.5 no.4
    • /
    • pp.234-237
    • /
    • 1995
  • Production of new flavanol derivatives with cytotoxic activity, gericudranin A and B, was studied by using hairy root cultures of Cudrania tricuspidata. Schenk and Hildebrandt (SH) medium was chosen for root growth and gericudranin production. After 35 days culture in a half-strength liquid SH medium containing $30g^{glucose}$/l, hairy root growth reached $138g^{FW}$/I and gericudranin A and B were produced at concentrations of 27mg/l and 21 mg/l, respectively. It was also observed that the contents of gericudranin A and B in hairy root were eight and six times higher than those of cudraniae radix, respectively.

  • PDF

Graded approach to determine the frequency and difficulty of safety culture attributes: The F-D matrix

  • Ahn, Jeeyea;Min, Byung Joo;Lee, Seung Jun
    • Nuclear Engineering and Technology
    • /
    • v.54 no.6
    • /
    • pp.2067-2076
    • /
    • 2022
  • The importance of safety culture has been emphasized to achieve a high level of safety. In this light, a systematic method to more properly deal with safety culture is necessary. Here, a decision-making tool that can apply a graded approach to the analysis of safety culture is proposed, called the F-D matrix, which determines the frequency and the difficulty of safety culture attributes recently defined by the IAEA. A hierarchical model of difficulty contributors was developed as a scoring standard, and its elements were weighted via expert evaluation using the analytic hierarchy process. The frequency of the attributes was derived by analyzing reported events from nuclear power plants in the Republic of Korea. Period-by-period comparisons with the F-D matrix can show trends in the change of the maturity level of an organization's safety culture and help to evaluate the effectiveness of previously implemented measures. In the evaluating the difficulty of the attributes in the recently developed harmonized safety culture model, the difficulties of Trending, Benchmarking, Resilience, and Documentation and Procedures were found to be relatively high, while the difficulties of Conflicts are Resolved, Ownership, Collaboration, and Respect is Evident were found to be relatively low. A case study was conducted with an analysis period of 10 years to attempt to reflect the many changes in safety culture that have been made following the Fukushima accident in March 2011. As a result of comparing two periods following the Fukushima accident, the overall frequency decreased by about 40%, providing evidence for the effects of the various improvements and measures taken following the increased emphasis on safety culture. The proposed F-D matrix provides a new analytical perspective and enables an in-depth analysis of safety culture.

Preparation of Epoxidized Soft Terpolymers and Their Reactive Compatibilizing Effects on PP/EVOH Blends

  • Kim, Jung Soo;Jeon, Dong Gyu;Jang, Ji Hoon;Kim, Jin Hoon;Kim, Ki Bum;Yang, Hong Joo;Park, Jun Sung;Lee, Youn Suk;Kim, Dong Hyun
    • Elastomers and Composites
    • /
    • v.50 no.3
    • /
    • pp.189-195
    • /
    • 2015
  • In this study, we prepared epoxidized poly ethylene-ter-1-decene-ter-divinylbenzene (Epo-PEHV) as a reactive compatibilizer to prevent phase separation phenomenon which occurs upon blending polypropylene (PP) and ethylene-vinyl alcohol copolymer (EVOH). Firstly, PEHV was prepared under high catalyst activity according to content of catalyst and cocatalyst. After then, we modified vinyl group of the terpolymer with epoxy group. We observed that the terpolymer was successfully epoxidized by 1H-NMR and FT-IR analysis. The Epo-PEHV was added by 2, 5, 10% in PP/EVOH blends. The morphologies and mechanical properties of PP/Epo-PEHV/EVOH blends were analyzed by SEM and UTM, respectively. Epo-PEHV enhanced the interfacial adhesion of PP and EVOH blends.