• Korean Journal of Veterinary Research
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• v.42 no.1
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• pp.55-64
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• 2002

The envelope glycoprotein of HIV-1 forms an oligomeric complex resulting in playing a role to induce neutralizing antibody and cell-mediate immune responses. The oligomer exists as a trimer of gp120-gp41 heterodimer which mediates HIV-1 attachment and fusion. We made a cDNA clone of gp140 consisting of gp120 and ectodomain of gp41 from the primary African isolate. To express the oligomeric gp140 in mammalian cells, we adopted the Semliki Forest virus (SFV) based expression system. The oligomeric gp140 in the secretory form was expressed and purified from the cell culture supernatant and characterized. The antibody inducing activity of the purified gp140 was also examined in mice inoculation.

• Korean Journal of Microbiology
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• v.25 no.4
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• pp.293-303
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• 1987

A $C_{1}$ enriched cellulase fraction was separated from culture filtrate of anaerobic Clostridium thermocellum by hydroxyapatite column chromatography. The separated fraction showed strong synergistic action with $C_{x}$ component (endo-$\beta$-1, 4-glucanase) in digestion of crystalline cellulose, similar to the other aerobic cellulolytic microorganisms. Unlike the $C_{x}$ component the $C_{1}$ enriched fraction was rapidly inactivated by oxidation at the atmospheric condition. The enzyme activity was significantly enhanced by the addition of reducing agents, especially $\beta$-mercaptoethanol, which indicates that a $C_{1}$ component has a lot of sulfhydryl groups essential for the enzyme activity. The effect of metal ions on $C_{1}$ activity was also investigated. The $C_{1}$ fraction was found to be thermally stable compare to endo-$\beta$-1,4-glucanase. Optimal temperature and pH were found to be $60^{\circ}C$ and 6.0, respectively.

• Korean Journal of Plant Tissue Culture
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• v.24 no.3
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• pp.161-165
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• 1997

The R-mb locus of maize is one of several genes that encode tissue-specific transcriptional regulator for the anthocyanin biosynthesis in plant parts and the aleurone layer in seeds. We found that the seed pigment frequencies gradually decreased at selfed progenies of the R-mb genetic stocks. In order to analyze the genomic structure of R-mb locus components, genomic Southern blot was performed by using R specific probe, pR-nj:1. Two bands were detected at the size of about 3.9 and 7.75kb. Five R-mb positive clones (mb-II, III, V,Ⅵ, and Ⅶ) were obtained by screening of maize genomic λFIXII library using R specific probe pR-nj:1. We constructed the restriction map of clone mb-II (7.75Kb positive) and mb-Ⅵ (3.9Kb positive), and have compared these with other R locus genes. From genetic and molecular analysis, it is suggested that R-mb complex consists two copy of R elements, and each element shows the paramutagenic and gene silencing effects by the fashion of cis-inactivation.

• Cross-Cultural Studies
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• v.20
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• pp.113-148
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• 2010

This paper examines the American role in shaping South Korean nation-building during the early Cold War by considering how the United States attempted to form South Korea's education and how Koreans responded to these efforts. It looks at education as an arena where "American" ideas such as democracy and liberalism were received, transformed, and utilized by Koreans. This study pays particular attention to the gap between American intentions and Korean expectations, as well as to the competition between American and Japanese systems, which explains the contradictory role America played in South Korean nation-building. In order to better assess the role of the United States in shaping South Korean education, this article considers the complex dynamics between the Japanese legacies, American influence, and Korean actors. Americans had exerted a great effect on Korean education since the beginning of their relationship. American missionaries, U.S. military government, and educational mission teams had all contributed to the expansion of educational opportunities for Koreans. Through the educational institutions that they established or helped establish, Americans tried to spread "their" ideas. In this process, Americans had to struggle with two obstacles: Korean nationalism and the legacies of Japanese colonialism. Many Koreans used American missionary schools for their own purposes and resisted U.S. military government's policies which ignored their desire for self-determination. American education missions had limited effect on Korean education due to the heterogeneous Japanese system that was still influencing South Korea even after liberation. The ways in which Americans have influenced the democratization of South Korea have not been simple. Although "American" democratic ideas reached Koreans through various routes, Koreans understood the "American" idea within their own historical context and in a way that fit their existing socio-political relations. Oftentimes suspicious of "American" democracy, Koreans developed their own concept of democracy. The overall American influence on Korean democratization, as well as on Korean education, was important but limited. While Americans helped Koreans build educational infrastructure and tried to transfer democratic ideas through it, Koreans actors and Japanese colonial legacies limited its impact.

• Animal cells and systems
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• v.16 no.3
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• pp.207-214
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• 2012

Burns are one of the most devastating forms of trauma and wound healing is a complex and multicellular process, which is executed and regulated by signaling networks involving numerous growth factors, cytokines, and chemokines. Recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF) was specifically produced from rice cell culture through use of a recombinant technique in our laboratory. The effect of rhGM-CSF on promotion of deep second-degree burn wound healing on the back skin of a hamster model was evaluated through a randomized and double-blind trial. As macroscopic results, hamster skins of the experimental groups showed earlier recovery by new epidermis than the control groups. Immunohistochemical reactions of proliferating cell nuclear antigen and transforming growth factor-b1, which are indicators of cell proliferation, were more active in the experimental group, compared with the control group. On electron microscopy, basal cells in the epidermis of the experimental group showed oval nuclei, prominent nucleoli, numerous mitochondria and abundant free ribosomes. In addition, fibroblasts contained well-developed rough endoplasmic reticulum with dilated cisternae. Bundles of collagen fibrils filled the extracellular spaces. Particularly, ultrastructural features indicating active metabolism for regeneration of injured skin at 15 days after burn injury, including abundant euchromatin, plentiful free ribosomes, and numerous mitochondria, were observed. These findings suggest that use of rhGM-CSF could result in accelerated deep second-degree burn wound healing in animal models.

• Journal of the Korea Society of Computer and Information
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• v.25 no.4
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• pp.63-70
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• 2020

In this paper, we propose a globally successful animation rhythm type. Based on the study of spatial variables in Campbell's heroic narrative and the study on the duration of shots in the video, the rhythm of the shot according to the spatial narrative of was analyzed. Through this, three conclusions were drawn. First, the basis for the visualization of narrative intensity is possible through the shot density of narrative, Second, a shot rhythm type was presented, which was presented as an ascending type, a descending type, a mountain type, a depressed type, and a complex type, and the characteristics of the narrative were analyzed. Third, the strength of the shot rhythm shown in the hero narrative spatial map model was divided into top, middle, and bottom, and the measurement criteria of narrative strength were presented. This study is meaningful in that it visualized and typified artistic emotions as objective data in the flow of animation content focused on philosophical and qualitative methods.

• Journal of the Korea Convergence Society
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• v.12 no.10
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• pp.121-128
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• 2021

To investigate the relationship between mental health and oral health problems in the elderly, this study was conducted using raw data from the 7th 3rd(2018) National Health and Nutrition Examination Survey, targeting 753 elderly people aged 65 and over. For data analysis, complex sample analysis was performed using IBM SPSS 22.0 program. In the case of chewing problem, the odds ratio increased in the group with high stress perception and depression(p<0.05). In the case of speaking problem, the odds ratio increased in the group that received mental health counseling(p<0.05). As a result of this study, it was found that there is a relationship between mental health and oral health problems in the elderly. Therefore, it is thought that it is necessary to improve the quality of life through the development of programs to improve the mental health of the elderly and oral health problems.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.23 no.6
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• pp.690-696
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• 2019

New companies expecting market penetration by adopting technology commercialization process could face high uncertainty and constraints. In particular, ICT-based firms that enter competitive markets must deal with more complex situations. Although in-depth research has been done to solve the problem, there is still a lack of understanding of how startups' technology commercialization process is successful. To discuss the issue, this paper presents the technology commercialization model and determinants.(policy, finance, work team, organizational culture, overcoming the difficulties etc.) the suitability of the framework. From this analysis it emerged that the sustainability of organizational capabilities is as important as the adherence to the technology commercialization process for companies. As the organizational capacity has decreased, the driving force for technology commercialization has weakened. Therefore, the technology commercialization process does not guarantee the success of the market entry but is understood as a means of market access. If the organizational capacity is not strengthened, there is no successful technology commercialization process.

• Fisheries and Aquatic Sciences
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• v.24 no.4
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• pp.153-162
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• 2021

In this study, a fish metabolic accelerator (a combination of butaphosphan and cyanocobalamin [BPC]) was injected into the muscle of the olive flounder, Paralichthys olivaceus, to investigate its effect on immunity and stress in fish maintained at low temperatures. A single dose of BPC was injected (100 mg/kg body weight) into the olive flounder, and its immunity and stress were observed after one and two weeks. Immunity tests revealed the presence of lysozyme (LZM), nitroblue tetrazolium (NBT), myeloperoxidase (MPO), anti-protease (AP), glutathione peroxidase (GPx), and total immunoglobulin (TIg). BPC injection was found to increase immunity activity compared to the control group. In particular, there was significantly high GPx activity. There was similarly high activity for MPO and GPx in the first week following the injection, followed by significant differences between the BPC-injected and control groups in the second week. There was a reduced low water-temperature stress response in the BPC-injected fish, as evidenced by the cortisol and glucose levels of the control and BPC groups. Lower levels were also observed in the BPC group than the control group during the second week. Cortisol levels were significantly lower in the BPC group than the control group. Histological examinations were conducted in the first and second weeks after the intramuscular injection of the recommended BPC dose to confirm the safety of BPC in aquaculture. There were no abnormalities observed in any tissue samples. This study confirms that the injection of BPC is safe even when used in a culture situation. BPC helps relieve stress and improves non-specific immune responses (innate immunity) in the olive flounder.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.35 no.8
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• pp.3-14
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• 2019

The research is to track the morphological transformation of residential towers of apartment complexes in Busan. Though apartment is one of the most important components in Korean society, it has not drawn enough attention from academia as well as the public, especially regarding its shape. In result, its morphological change has barely been recognized. Instead, negative, but superficial, evaluation has become dominant tendency to deal with the shape of apartment tower. There are numerous 'partial' evidences to inform the morphological change of apartment plan type, and understanding the change is critical since morphological resultant reflects culture, politics, economy of a society. The research has been done on the 261 apartment complexes built through 2016 in Busan. The analysis shows that $I^0$ type, to represent 'typical boring architectural shape' of Korean apartments, has been dominant until the year 2000. However, its portion has become drastically decreased since the mid-2000. Composite plan type, the geometrical precedents of which had never been existent, emerged in the mid-2000, and this phenomenon may symbolize the change of morphology of apartment. When tower plan types are grouped together according to geometrical shape, three types, I-type, L-type and Y-type, take the most of residential towers, explaining 97% of the total. As expected, I-type is the most dominant, but its influence has decreased as time passes. Y-type might become one of the most important type in the period V. L-type has become the leading type in the Period VI to exceed I-type by 12 percent.