• Journal of Plant Biotechnology
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• v.36 no.4
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• pp.309-319
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• 2009

Transgenic plant cell cultures for the production of biopharmaceuticals including monoclonal antibodies, recombinant proteins have been regarded as an alternative platform in addition to traditional microbial fermentation and mammalian cell cultures. Plant-made pharmaceuticals (PMPs) have several advantages such as safety, cost-effectiveness, scalability and possibility of complex post-translational modifications. Increasing demand for the quantity and diversity of pharmaceutical proteins may accelerate the industrialization of PMP technology. Up to date, there is no plant-made recombinant protein approved by USFDA (Food and Drug Administration) for human therapeutic uses due to the technological bottlenecks of low expression level and slight differences in glycosylation. Regarding expression levels, it is possible to improve the productivity by using stronger promoter and optimizing culture processes. In terms of glycosylation, humanization has been attempted in many ways to reduce immune responses and to enhance the efficacy as well as stability. In this review article, all these respects of transgenic plant cell cultures were summarized. In addition, we also discuss the general characteristics of plant cell suspension cultures related with bioreactor design and operation to achieve high productivity in large scale which could be a key to successful commercialization of PMPs.

• The Plant Pathology Journal
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• v.30 no.2
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• pp.151-158
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• 2014

Three double-stranded RNAs (dsRNAs), approximately 1.85, 1.65 and 1.27 kb in size, were detected in an isolate of Cytospora sacchari from Iran. Partial nucleotide sequence revealed a 1,284 bp segment containing one ORF that potentially encodes a 405 aa protein. This protein contains conserved motifs related to RNA dependent RNA polymerases (RdRp) that showed similarity to RdRps of partitiviruses. The results indicate that these dsRNAs represent a novel Partitivirus that we tentatively designate Cytospora sacchari partitivirus (CsPV). Treatment of the fungal strain by cyclohexamide and also hyphal tip culture had no effect on removing the putative virus. Phylogenetic analysis of putative RdRp of CsPV and other partitiviruses places CsPV as a member of the genus Partitivirus in the family Partitiviridae, and clustering with Aspergillus ochraceous virus 1.

• Fashion & Textile Research Journal
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• v.6 no.5
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• pp.539-548
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• 2004

The purpose of this study is to know outer and inner characteristics of Bobos style regarded the latest fashion and to estimate the characteristics of fashion trend in 21st century and direction of fashion in the future, In terms of contentions of this study, we firstly studied concept, background, general characteristics which had been introduced in Sociology. And then, we studied the effect which the style of Bobos had been given by investigating the concept and style of Hippies and Yuppies. Also, we analysed trend of Bobos represented in modem fashion and tried to examine outer and inner features of Bobos through the result of analysis. Bobos can defined as 'Bourgeois Bohemian' who have both mundane desire about success and are high-educated elite class in information-oriented society. The fashion styles which had affected Bobos were psychedelic, naturalism, unisex, ethnic, layered, grunge of Hippies in 1960s and traditional, power-look, contemporary-look, sports wear, casual wear of Yuppies in 1980s. As a result of examining features of Bobos style, we can know that outer feature of Bobos style showed a reasonable harmony regardless of formalities and inner features showed a tendency to seek comforts. Also, Bobos style showed various and free styles, textile materials and colors and complex features to transcend the time and region, and culture.

• Journal of the Korean association of regional geographers
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• v.9 no.3
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• pp.248-261
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• 2003

Through a qualitative analysis of the Ikuno Korean Festival in Osaka, this article examines and critiques how identities are constructed, and how this process is shaped by the mediation of intra and inter-community concerns. Particular attention is paid to the potential of reorganized culture through a thinking of similarity rather than difference. The dynamic interrelations suggest that festival provides a particular and informal public sphere wherein certain social logics and identities are contested. These discursive arenas are therefore marked by certain exclusions and inclusions. This study shows the complex process of identification at the micro-level through which identification is constituted and continuously negotiated.

• The Journal of Natural Sciences
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• v.8 no.2
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• pp.199-224
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• 1996

The value orientations and life styles which are characterized by the postmodern societies have been expanding in korean society. This paper concerns with the postmodernism for in fashion design : at first the complex relationships between the logic of post-industrial society and the culture dynamics of postmodernism will be explained. Then the characteristics of postmodernism in the comparision with the Bauhaus, also "modernism" in design areas will be critically identified, and significant examples which I select in order to criticize the basic elements or orientations of postmodern design will be analized in details.I will make a new perspective which is beyond Bauhaus and postmodernism ; namly 'open dialogism design.'. But in the boundary of its introduction.

• Journal of the Korean housing association
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• v.19 no.6
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• pp.137-144
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• 2008

Public housing problem became a major issue in each European country during the period of the reconstruction. This study focuses on the one of the major institutional policies in the public housing sectors in Europe during the second postwar period, specifically the INA (Istituto Nazionale delle Assicurazioni)-Casa in Italy from 1949 to 1963. Thanks to the financial Aid provided by 'Marshall Plan' of United States government, Italy initiated INA-Casa housing program elaborated and realized by minister of Labour, Amintore Fanfani. He was strongly influenced by catholic socialism and Weberian corporatism, and also had previously written theoretical studies on the subject during the Interwar years. The outcome of this policy had been devaluated for its Keynesian aspect as well as vernacular predominance in the architectural forms. On the contrary, this study focuses on themes such as continuity of politics before and after the Liberation, status of the architects and their will to social engagement, notion of quarter as 'self-sufficient community' and 'residential unit'. These factors will allow to comprehend the complex nature of the reality where different categories were involved to resolve the common housing problem.

• Korean Institute of Interior Design Journal
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• v.23 no.4
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• pp.52-62
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• 2014

The rapid growth of economy improves not only the life quality of people in this modern society but also standards of value in connection with how those people would spend money as they engage in various leisure and cultural activities. That being the case, hotels are currently changing into places of new concepts depending on those new lifestyles of these people, and in those new places, people can entertain and experience as enjoying cultures. The marketing collaboration of the hotels with brands can be used as a more inclusive way to enhance brand images. In addition, as having infinite possibilities of the collaboration's being able to create a new-concept space with an identity of a brand included, this collaboration makes it possible for the design hotels to decorate their interior spaces differently from those of other hotels. In the light of that, the brand collaboration is basically divided into three groups which are the fashion collaboration, the technique collaboration and the designer collaboration, and the brand collaboration is also able to express the interior spaces of the design hotels as working on those six components, such as reproduction, events, alteration, messages, culture and image. After all, through the brand collaboration marketing, this thesis expects an interior design of a new-concept design hotel which would play a role as a complex cultural space.

• Fashion & Textile Research Journal
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• v.1 no.1
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• pp.62-68
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• 1999

The study has examined about the origin and change of traditional Japanese dress "Kosode" and classified it according to its ages and also classified its patterns which appeared on the Kosode by its kinds, and divide the patterns with constituent method and expressional method and so, studied and analyzed them with type of patterns according to the change of the times. The result of research were as follows: 1. Subject of patterns that appeared on the Kosode was used with mainly plant pattern, animals pattern, pictorial pattern, artificial pattern, and abstract patterns. 2. Expressional method of this patterns was mostly expressed simply with tie dyeing in ancient times, but it changed complex its expressional method and diversely due to the development of common's culture as time goes on. 3. For the arrangement of its pattern were arranged regularly mostly make demarcate and though they are as if seemed dispersed, but a characteristic that can find regulations in it. 4. Transition of patterns that appeared on the Kosode it developed at the beginning of ancient syouboon, Katasuso pattern and Katamigaori patterns at the samurai's society in the medieval society and developed to Koicho kosode and Kambun kosode in modern times.

• Journal of the Korea Society of Computer and Information
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• v.12 no.1 s.45
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• pp.199-204
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• 2007

The objective of this study is to interpret that at international business management, overseas marketing research is very important to enter the new market as well as competitive market in the wold. There are many distinct components of the environment which can affect the exporting company, so the exporting company must to find the opportunities and threats in foreign market after it researches the firms export potential for successful or survival themselves. Often the export marketing research is a more difficult and complex task than the domestic marketing research. As international marketing activities for exporting company, an investigator has to get abilities for understanding regional culture and to analyze the data for investigation and findings as skeptical mind.

• Journal of Environmental Health Sciences
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• v.26 no.4
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• pp.88-96
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• 2000

The aim of the current study was to evaluate the transport system in EMT-6 cell for the uptake of the methylmercury(MeHg). Several inhibitors ere used to test used to test which potential transport system might be involoved in MeHg uptake. Probenecid was used to test the organic transport system, valinomycin for testing the effect of the membrane potential, cytochalasin B for testing the facilitated diffusive D-glucose transport system and colchicine for testing the microtubule system. Ouabain for evaluating active transport system, 4',4-diisothiocyano-2',2-stilbenedisulfonic acid(DIDS) the Cl- ion transport system and verapamil for the $Ca^{2+}$ transprot system. Significantly, MeHg decreased the synthesis of nitric oxcide(NO) and intracellular ATP in ENT-6 cells. In the condition of ouabain containing with MeHg decreased the production of NO and intracelluar ATP. In the treatment of inhibitors, ouabain showed protective effect against cytotoxicity of MeHg but ather inhibitors not showed protective effects. The protective effects of ouabain against the cytotoxicity of MeHg deoended on the concentration of added ouabain to the culture medium for MET-6 cells. These result showed that the uptake of MeHg might be involved in the active transport system. Active transports system seems to share similarities with the transport systems for the uptake of MeHg when using MeHg and MeHg-glutathione complex.x.