• 제목/요약/키워드: Culture Collection

검색결과 888건 처리시간 0.025초

Comparison of Three Different Methods for Campylobacter Isolation from Porcine Intestines

  • Shin, Eun-Ju;Lee, Yeon-Hee
    • Journal of Microbiology and Biotechnology
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    • 제19권7호
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    • pp.647-650
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    • 2009
  • Using 200 porcine colon tissues, the efficiencies of three isolation methods of Campylobacter from porcine intestines were compared: Method 1, direct streaking of colon mucosa; Method 2, direct inoculation of intestinal contents with a swab; Method 3, inoculation of pre-enriched medium. A total of 460 Campylobacter isolates were obtained from 178 samples (89%) by direct streaking of colon mucosa, 142 samples (71%) by direct streaking of a swab, and 94 samples (47%) by pre-enrichment of intestinal contents in Preston broth. Direct streaking of colon mucosa was superior to the other two isolation methods, in terms of rapidity and higher efficiency. When isolates were identified with various biochemical tests and PCRs specific to 16s rRNA, mapA, and ceuE, C. coli was the predominant species (87%) in porcine, whereas the rest of the isolates were identified as C. lanienae.

2005~2009년 콜렉션에서 선보인 텍스타일 디자인 고찰 및 패턴 디자인 개발 - 컨버세이셔널 디자인을 중심으로 - (Observation of Textile Design from 2005~2009 Fashion Collection and Development of Pattern Design - Focused on Conversational Design -)

  • 김칠순;박지은
    • 복식문화연구
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    • 제18권6호
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    • pp.1179-1193
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    • 2010
  • For a good design, it is important to understand consumers and trend of textile and fashion design. Previous researches on textile pattern design with CAD have been done. They have studied on Korean traditional floral motives/hand painting or some geometric patterns. Few studies have been done on conversational design. Therefore the purpose of study was to observe types of textile pattern design for the recent fiver years and determine fabric pattern trends, especially on the conversational motifs. The types of patterns were analysed with professional panels from the 2005~2009 collection sources, using SPSS program. This study was also develop textile patterns creating using CAD. We conducted design process on motif creating, repeat, and mapping jobs, using textile special design program Prima vision and Photoshop CS3.

Notes on Cordyceps species Collected from the Central Region of Nepal

  • Shrestha, Bhushan;Sung, Jae-Mo
    • Mycobiology
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    • 제33권4호
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    • pp.235-239
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    • 2005
  • The present study was carried out to explore the Cordyceps species and other entomopathogenic fungal flora around Kathmandu Valley and a few high altitude locations of Nepal. In this paper, we report eight Cordyceps species as new to Nepal: C. gracilis, C. ishikariensis, C. liangshanensis, C. martialis, C. militaris, C. pruinosa, C. sphecocephala and C. tricentri. We also mention a few allied genera such as Beauveria, Hirsutella and Paecilomyces from Nepal. Further collections from different ecological regions of Nepal will show the richness of entomopathogenic fungal floral diversity of Nepal.

동물에서 분리된 용혈성 대장균의 항생제 내성 (Antibiotic Resistance of Hemolytic Escherichia coli Isolated from Animals in Korea)

  • 이계남;박용호;정병열;이연희
    • 미생물학회지
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    • 제39권4호
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    • pp.293-295
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    • 2003
  • Total 70 isolates of Escherichia coli obtained from pigs were studied. Forty four isolates had $\textregistered$-hemolytic activity which was heat labile. Minimum inhibitory concentration test indicated that 40 isolates (57.1%), 15 isolates (21.4%), 23 isolates (32.9%), and 5 isolates (7.1%) were resistant to ampicillin, cephalothin, gentamicin, and norfloxacin, respectively. None of them were extended spectrum $\textregistered$-lactamases (ESBLs) producer when the double disk synergy test (DDST) was performed.

Detection of Extracellular Enzyme Activity in Penicillium using Chromogenic Media

  • Yoon, Ji-Hwan;Hong, Seung-Beom;Ko, Seung-Ju;Kim, Seong-Hwan
    • Mycobiology
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    • 제35권3호
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    • pp.166-169
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    • 2007
  • A total of 106 Penicillium species were tested to examine their ability of degrading cellobiose, pectin and xylan. The activity of ${\beta}$-glucosidase was generally strong in all the Penicillium species tested. P. citrinum, P. charlesii, P. manginii and P. aurantiacum showed the higher ability of producing ${\beta}$-glucosidase than other tested species. Pectinase activity was detected in 24 Penicillium species. P. paracanescens, P. sizovae, P. sartoryi, P. chrysogenum, and P. claviforme showed strong pectinase activity. In xylanase assay, 84 Penicillium species showed activity. Strong xylanase activity was detected from P. megasporum, P. sartoryi, P. chrysogenum, P. glandicola, P. discolor, and P. coprophilum. Overall, most of the Penicillium species tested showed strong ${\beta}$-glucosidase activity. The degree of pectinase and xylanase activity varied depending on Penicillium species.

바이오뱅크 분야의 연구데이터 관리 및 서비스 동향에 관한 연구 (Study on the data management and service trends in the biobank)

  • 박미영;안인자;안경숙
    • 한국정보관리학회:학술대회논문집
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    • 한국정보관리학회 2017년도 제24회 학술대회 논문집
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    • pp.98-98
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    • 2017
  • 본 연구의 목적은 바이오뱅크(생명연구자원 소재은행)분야의 연구데이터 관리 및 서비스 동향을 고찰하고 시사점을 도출하는데 있다. 바이오뱅크 분야의 연구데이터 관리 및 서비스와 관련하여 해외 선진 사례를 프로그램과 서비스 모델측면에서 조사하였다. 조사된 프로그램과 서비스 모델은 ATCC(American Type Culture Collection), BIobank Data Sharing (MIABIS 2.0 Core), WDCM(World Data Center for Microorganism/Word Federation for Culture Collection)이다. 각각의 프로그램과 서비스 모델에서 시사점을 도출하였으며, 본 연구에서는 이를 기반으로 국내 생명연구자원 바이오뱅크 분야의 연구데이터 관리 및 서비스를 위한 시사점을 제시하였다.

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Validity of bag urine culture for predicting urinary tract infections in febrile infants: a paired comparison of urine collection methods

  • Kim, Geun-A;Koo, Ja-Wook
    • Clinical and Experimental Pediatrics
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    • 제58권5호
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    • pp.183-189
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    • 2015
  • Purpose: Catheter urine (CATH-U) and suprapubic aspiration (SPA) are reliable urine collection methods for confirming urinary tract infections (UTI) in infants. However, noninvasive and easily accessible collecting bag urine (CBU) is widely used, despite its high contamination rate. This study investigated the validity of CBU cultures for diagnosing UTIs, using CATH-U culture results as the gold standard. Methods: We retrospectively analyzed 210 infants, 2- to 24-month-old, who presented to a tertiary care hospital's pediatrics department between September 2008 and August 2013. We reviewed the results of CBU and CATH-U cultures from the same infants. Results: CBU results, relative to CATH-U culture results (${\geq}10^4$ colony-forming units [CFU]/mL) were widely variable, ranging from no growth to ${\geq}10^5CFU/mL$. A CBU cutoff value of ${\geq}10^5CFU/mL$ resulted in false-positive and false-negative rates of 18% and 24%, respectively. The probability of a UTI increased when the CBU bacterial count was ${\geq}10^5/mL$ for all infants, both uncircumcised male infants and female infants (likelihood ratios [LRs], 4.16, 4.11, and 4.11, respectively). UTIs could not be excluded for female infants with a CBU bacterial density of $10^4-10^5$ (LR, 1.40). The LRs for predicting UTIs based on a positive dipstick test and a positive urinalysis were 4.19 and 3.11, respectively. Conclusion: The validity of obtaining urine sample from a sterile bag remains questionable. Inconclusive culture results from CBU should be confirmed with a more reliable method.

중국 신장 위구르족 복식의 특성을 활용한 패션 디자인 (Development of fashion design applied to costume of the Chinese Minority Xinjiang Uygur)

  • 왕리봉;이연희
    • 복식문화연구
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    • 제28권4호
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    • pp.492-507
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    • 2020
  • This study aims to develop fashion designs that combine atlas fabric with the characteristics of Uygur costume to modernize the costume of the Xinjiang Uygur. Research contents and methods are as follows. First, based on previous studies, research analysis was conducted on the cultural background, clothing characteristics, and material of Uygur clothing. Second, based on such research contents, designs combining the characteristics of Uygur costume and atlas fabric were presented. Third, to analyze the utilization of atlas fabric and examine fabric characteristics, material was gathered from collections on domestic and foreign web sites. Through field explorations of local museums in the Xinjiang area, minority group culture was observed in more detail. Based on collection of traditional clothing and analysis of its characteristics, fashion designs that apply contemporary trends were developed. General silhouettes without any restrictions to the waist and decorations made using embroidery were often used. Atlas silk, developed in China using Ikat weaving methods, is an important traditional clothing fabric of the minority group Xinjiang. Based on such data collection analysis, the produced works highlighted traditional ethnic characteristics by extracting classical patterns of atlas fabric, modifying or partially expanding them, combining them with hand knitting, and adding contemporary sensations, thus providing confirmations of the possibility of popularizing classic patterns in more practical manners.