• Title/Summary/Keyword: Culture Collection

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Aspergillus Associated with Meju, a Fermented Soybean Starting Material for Traditional Soy Sauce and Soybean Paste in Korea

  • Hong, Seung-Beom;Kim, Dae-Ho;Samson, Robert A.
    • Mycobiology
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    • v.43 no.3
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    • pp.218-224
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    • 2015
  • Aspergillus is an important fungal genus used for the fermentation of Asian foods; this genus is referred to as koji mold in Japan and China. A. oryzae, A. sojae, and A. tamari are used in the production of miso and shoyu in Japan, but a comprehensive taxonomic study of Aspergillus isolated from Meju, a fermented soybean starting material for traditional soy sauce and soybean paste in Korea, has not been conducted. In this study, various Aspergillus species were isolated during a study of the mycobiota of Meju, and the aspergilli were identified based on phenotypic characteristics and sequencing of the ${\beta}$-tubulin gene. Most strains of Aspergillus were found to belong to the following sections: Aspergillus (n = 220), Flavi (n = 213), and Nigri (n = 54). The most commonly identified species were A. oryzae (n = 183), A. pseudoglaucus (Eurotium repens) (n = 81), A. chevalieri (E. chevalieri) (n = 62), A. montevidensis (E. amstelodami) (n = 34), A. niger (n = 21), A. tamari (n = 15), A. ruber (E. rubrum) (n = 15), A. proliferans (n = 14), and A. luchuensis (n = 14); 25 species were identified from 533 Aspergillus strains. Aspergillus strains were mainly found during the high temperature fermentation period in the later steps of Meju fermentation.

Transfer of Antimicrobial-Resistant Escherichia coli and Resistance Genes in a Child Care Center

  • Hong, Hyunjin;Lee, Yeonhee
    • Journal of Microbiology and Biotechnology
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    • v.29 no.3
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    • pp.465-472
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    • 2019
  • Several reports describe antimicrobial-resistance transfer among children and the community in outbreak situations, but transfer between a child and a caregiver has not been examined in child care facilities under normal circumstances. We investigated the transfer of antimicrobial-resistance genes, resistant bacteria, or both among healthy children and teachers. From 2007 to 2009, 104 Escherichia coli isolates were obtained from four teachers and 38 children in a child care center. Twenty-six cephem-resistant isolates were obtained from children in 2007 and 2008. In 2009, cephem-resistant isolates were detected in children as well as a teacher. Nalidixic acid-resistant isolates from the same teacher for 3 years showed low similarity (<50%) to each other. However, an isolate from a teacher in 2007 and another from a child in 2008 showed high similarity (87%). Pulsed-field gel electrophoresis revealed 100% similarity for four isolates in 2007 and one isolate in 2008, and also similarity among seven isolates carrying the virulence gene (CNF1). This study yielded the following findings: (1) a gene for extended-spectrum ${\beta}$-lactamase was transferred from a child to other children and a teacher; (2) a nalidixic acid-resistant isolate was transferred from a teacher to a child; and (3) a virulent bacterium was transferred between children.

Relationship between University students' Marriage View and Quality of Life

  • Kim, Jungae;Cho, Euiyoung
    • International Journal of Advanced Culture Technology
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    • v.8 no.4
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    • pp.129-136
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    • 2020
  • This study was a cross-sectional descriptive study that analyzed the effect of quality of life on marriage views in college students. The purpose of this study was to identify the relationship between quality of life and marriage view. The study targets were 195 male and female university students aged between 20 and 25. The data collection was done only by those who voluntarily agreed to the study using online questionnaires, and the data collection period was from October 1 to October 30, 2020. Data analysis was performed using SPSS 18.0 for frequency analysis, χ2 analysis, Pearson correction, t-test, and simple regression analysis. The result of the study that men had higher conservative marriage view (M=3.22, SD=.716) than women (M=2.21, SD=.903, p<0.01), women had higher active marriages (M=3.94, SD=.955) than men (M=3.41, SD=.933, p<0.01), and women had higher exclusive marriage views (M=3.94, SD=.955) than men (M=3.41, SD=.933, p<0.01). And people with high quality of life positively influenced romantic marriage view (R2=.029, p<0.05), but negatively affected passive marriage view (R2=.029, p<0.05) and conservative marriage view (R2=.034, p<0.05). Based on the results of this study, a program is needed to create a positive marriage view for the married age group, create an environment for married people to positively engage in social and economic activities, and propose a program for creating healthy family relationships.

Phenomena of mixture in Sacai's knit design (사카이(Sacai) 니트 디자인에 나타난 혼합 현상)

  • Lee, Younhee;Kim, Hea Yeon
    • The Research Journal of the Costume Culture
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    • v.29 no.6
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    • pp.763-778
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    • 2021
  • The study aims to analyze the mixed characteristics of knit fashion design as demonstrated in Sacai's collection, which promotes hybrid and mesh-up fusion and proposes various knit design ideas. Standards for categorizing mixed phenomena of Sacai's knit design were established through a review of literature and data analysis. The study's data collection period was from 2012 to 2021, and a total of 174 items were analyzed. The following are the results. First is the fusion of traditional knit and contemporary styles expressed through de-constructive design; traditional knit styles are combined with contemporary styles through color, material, and knitting structure mixtures, such as mixing Northern European Aran, Fair Isle, Nordic, and Ropy knit. Second, the Sakai knit design combines male and female styles by mixing materials and details. A deconstructed knit cardigan was matched with androcentric pin-striped shirts worn over layered shirring skirts embellished with chiffon and lace. Third, everyday street fashion style is blended with a formal, elegant fashion style. For example, the front view has a typical everyday appearance, whereas the back view contains florid and decorated details. The style is expressed as a 360-degrees appellation, with one thing in the front and another in the back. Sacai's distinctive expressional characteristics include a mixed and de-constructive style characterized by unexpected design.

Young Children Teacher's Application Experience and Utilizing Observation of Digital Device

  • Kim, Dae-Wook;Song, Yun-Kyung
    • International Journal of Advanced Culture Technology
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    • v.10 no.4
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    • pp.126-136
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    • 2022
  • The purpose of this study is to investigate the application experience and observation of young children's teachers' digital devices. The participants of this study were 6 young children teachers using digital devices. Individual interviews were conducted as a data collection method. It was conducted 2-3 times per research participant. Data collection was conducted from November to December 2020. The Data analysis was focused on the young children's teachers' digital device application experience and observation use. 'Easy and simple use', 'using existing familiar apps', 'providing healing to teachers', and 'improving the utilization of spare time' were derived as early childhood teachers' experience of applying digital devices. 'Easy use of observation records', 'Use together in various ways', 'Use for parent counseling', 'Use appropriate for observation of revised Nuri Curriculum' through early childhood teacher's experience of observing digital devices ' was derived. As a conclusion of this study, first, it is easy for young children's teachers to apply digital devices to the field. Second, if young children's teachers use digital devices, they can make a wide range of observations, enabling high-quality early childhood education practices.

Characteristics of neo-deconstruction in Feng Chen Wang's Chinese fashion collection (펑첸왕 중국 패션 컬렉션에 나타난 신-해체주의 특성)

  • Anna Ren;Younhee Lee
    • The Research Journal of the Costume Culture
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    • v.31 no.6
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    • pp.806-823
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    • 2023
  • The aim of this study was to analyze the design characteristics of Chinese fashion designer Feng Chen Wang and interpret their implicit meaning from a neo-deconstruction perspective. A review of domestic and foreign literature, outlined the develop- ment of deconstruction and neo-deconstruction, with neo-deconstruction's aesthetic features termed 'traditional fusion', 'positive playfulness', 'open communication', and 'multiple inclusiveness'. These features informed an analysis of Feng Chen Wang's fashion design. Four key findings emerged. First, 'traditional fusion' combines traditional Chinese colors, items, handicrafts, and patterns with modern design to break down boundaries between past and modern, tradition and fashion. Second, 'positive playfulness' promotes creativity and fun, using bright colors, exaggerated accessories, and playing withthe composition of traditional clothes to create a positive atmosphere. Third, 'open communication' emphasizes design that combines practicality and creati- vity in response to consumer needs, incorporating the thoughts arising from individual experiences and interest in social phenomena. Fourth, 'multiple inclusiveness' breaks down boundaries of sexuality, hierarchy, and body shape, embracing various ideas of beauty and respecting uniqueness through design that are seen as available to all. Using a neo-deconstruction perspective, Feng Chen Wang provides novel product planning ideas for Chinese fashion brands and reflects the values and meaning of modern design pursued by contemporary Chinese designers.

Cryopreservation of Mulberry Germplasm Core Collection and Assessment of Genetic Stability through ISSR Markers

  • Rao, A. Ananda;Chaudhury, Rekha;Kumar, Suseel;Velu, D.;Saraswat, R.P.;Kamble, C.K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.15 no.1
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    • pp.23-33
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    • 2007
  • A simple and reliable cryo technique using desiccation and slow freezing of winter dormant buds was employed for 238 core collection of mulberry germplasm collected from diverse geographical regions and maintained under tropical conditions in the ex situ field gene bank to develop long-term biodiversity conservation for ensuring sustainable utilization of these valuable resources. Desiccation and freezing tolerance of bud grafts and excised shoot apices in the axillary buds of different Morus species under in vivo and in vitro condition indicated species-specific variation and most of the wild Morus species were found sensitive. In vitro regeneration and cryopreservation($-196^{\circ}C$) protocols using differentiated bud meristem like axillary winter dormant buds were worked out for a wide range of Morus species, land races, wild and cultivated varieties. Successful cryopreservation of mulberry winter dormant buds of different accessions belonging to M. indica, M. alba, M. latifolia, M. cathayana, M. laevigata, M. nigra, M. australis, M. bombycis, M. sinensis, M multicaulis and M. rotundiloba was achieved. Among wild species Morus tiliaefolia, and M. serrata showed moderate recovery after cryopreservation. Survival rates did not alter after three years of cryopreservation of different Morus species. ISSR markers were used to ascertain the genetic stability of cryopreserved mulberry, which showed no difference detected among the plantlets regenerated from frozen apices in comparison to the non-frozen material.

Aesthetic values of image fashion as fashion communication tool in the 21st century (21세기 패션커뮤니케이션 도구로서 영상 패션의 미학적 가치)

  • Kim, Sun Young
    • The Research Journal of the Costume Culture
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    • v.21 no.6
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    • pp.793-809
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    • 2013
  • This study examined the trend and aesthetic value of image fashion of the 21st century through empirical case study with image media as fashion communication tool. This research is intended to provide the basic material for both better comprehension of image fashion as emotional medium in the modern fashion and more activated research on interactivity and communication in the fashion communication area by examining how fashion designers deliver their design philosophy, thought and vision via image fashion. For the research method, the theoretical study was realized about image media and fashion communication with focus on related literature and precedent research. Also, the empirical study was performed about image fashion covering 2005 through 2012 by the group of fashion film, fashion animation, and video look-book. Fashion image in the research was developed into various layouts that include the story based or non-story based creative images, many kinds of episode or behind-the-scenes story in the prep course of collection, designer's personal life or product world, or re-cutting course after collection. It also played a communicative role for public relation of new product ad, concept conveyance of seasonal collections, means as online collection to substitute for the existing offline collections, and better understandings of designer or fashion brand. Aesthetic values derived from case analysis of image fashion in the modern fashion of 21st century appeared in the form of trans-boundary as convergence art, fantasy in harmony with virtuality and reality, and interactivity among the humans, image media and fashions.

A Study of Deformation Depicted on Moschino's Collection -Focusing on 2006~2010 Year Collection- (모스키노 컬렉션에 표현된 데포르마시옹에 관한 연구 -2006~2010년의 Collection을 중심으로-)

  • Lee, Jee-Yeon;Cho, Jean-Suk
    • The Research Journal of the Costume Culture
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    • v.19 no.3
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    • pp.488-500
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    • 2011
  • [ $D{\'{e}}formation$ ]is one of the most important cultural factor which puts people at ease and gives a sense of security. This study, in the process, analyzes the works of Moschino. Moschino's designs are rated to have approached the sublime when it comes to transforming the psychological anxiety of everyday living into a laughter. After selecting one hundred-two of Moschino's designs from the Internet Web site(www.cft.or.kr, www.samsungdesign.net), this study examines and analyzes the characteristics and types of deformation found in them. The result as follows. The examination of deformation found in Moschino designs can be classified into a transformation, distortion, exaggeration, and illusion. Transformation, a conscious change of the existing form or function, was shown as the change of an existing position, form, function and designation of a new function. Distortion, an interpretation away from the reality or a "wrong interpretation," was shown by placing opposing factors in left-right position as an extreme asymmetry. Exaggeration, always beyond the realm of reality, was shown thorough an enlargement or a magnification of a specific part and a repetition of a detail factors. Illusion, through a distortion of reality results in something that looks new, was expressed through the effects of wearing a two-pieces, an expression of details, effects of wearing accessories, and an expression of a dynamism. Therefore, Moschino has reflected the desire of homo modern to transform the existing situation through many techniques of deformation.

Comparison of the Bacterial Contamination Rates according to the Urine Collection Methods in Women (여성에서 소변채집방법에 따른 소변검체의 오염율 비교)

  • Jeong Ihn-Sook;Yang Man-Gil;Oh Hyang-Soon
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.6 no.3
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    • pp.359-368
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    • 1999
  • Background : The purpose of this study was to determine whether cleansing the perineum and urethral meatus and using midstream urine affect the rate of bacterial contamination of urine specimens, and to determine the optimum urine collection method. We studied 41 asymptomatic healthy nursing school students. Women who were menstruating were not excluded from this study. Method : The first and midstream urine samples were collected during consecutive urinationsby each woman. The first sample was not a clean-catch specimen, and the second one was a clean-catch specimen. Both specimens were studied by urinalysis and bacterial culture with standard methods. Results : 41 women met the study criteria and 39 successfully completed the study. None of the urine cultures were positive. 68.3% of the non clean-catch first urine cultures, 53.7% of the non clean-catch midstream cultures, 33.3% of the first clean-catch urine culteres and 30.8% of the midstream clean-catch urine were found to be contaminated. There was a significant difference in the bacterial contamination rates between the first and midstream urine, and the clean-catch and non clean-catch urine(p=0.035, p =0.001 respectively). On urinalysis, 7.3% of the non clean-catch first urine, 7.3% of the non clean-catch midstream urine, 2.6% of the clean-catch first urine and 2.6% of clean-catch midstream urine were found to be above grade 2. Conclusions : According to our results, the bacterial contamination rate was the lowest in midstream and clean catch urine specimens. Threrfore it is recommended that the midstream clean-catch technique is the standard practice for collecting urine specimens for bacterial culture in women.

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