• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.711-717
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• 2008

To speed up the conversion of rice straw into feeds in a low-temperature region, a start culture used for ensiling rice straw at low temperature was selected by continuous enrichment cultivation. During the selection, the microbial source for enrichment was rice straw and soil from two places in Northeast China. Lab-scale rice straw fermentation at $10^{\circ}C$ verified, compared with the commercial inoculant, that the selected start culture lowered the pH of the fermented rice straw more rapidly and produced more lactic acid. The results from denatured gradient gel eletrophoresis showed that the selected start culture could colonize into the rice straw fermentation system. To analyze the composition of the culture, a 16S rRNA gene clone library was constructed. Sequencing results showed that the culture mainly consisted of two bacterial species. One (A) belonged to Lactobacillus and another (B) belonged to Leuconostoc. To make clear the roles of composition microbes in the fermented system, quantitative PCR was used. For species A, the DNA mass increased continuously until sixteen days of the fermentation, which occupied 65%. For species B, the DNA mass amounted to 5.5% at six days of the fermentation, which was the maximum relative value during the fermentation. To the authors' best knowledge, this is the first report on ensiling rice straw with a selected starter at low temperature and investigation of the fermented characteristics.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.471-475
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• 2001

This experiment was conducted to evaluate the effect of slow-release fertilizer on warm type garlic cultivation in the Southwestern region of Korea. The slow-release fertilizers used were UF(Urea-Formaldehydes)70, 100, 130, CDU(Crotonylidene Diurea)100, and IBDU(Isobuty- lidene Diurea)100. The slow-release fertilizer treatment slightly increased plant height, leaf sheath diameter, and leaf number compare with conventional fertilizer treatment. Also, large bulb rate, nitrogen and pyruvic acid contents of garlic in slow-release fertilizer plot were higher than other treatments. Application of slow-release fertilizer increased the commercial yields.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.10
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• pp.1390-1394
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• 2004

We have achieved efficient transformation system for forage-type tall fescue plants by Agrobacterium tumefaciens. Mature seed-derived embryogenic calli were infected and co-cultivated with each of three A. tumefaciens strains, all of which harbored a standard binary vector pIG121Hm encoding the neomycin phosphotransferase II (NPTII), hygromycin phosphotransferase (HPT) and intron-containing $\beta$-glucuronidase (intron-GUS) genes in the T-DNA region. Transformation efficiency was influenced by the A. tumefaciens strain, addition of the phenolic compound acetosyringone and duration of vacuum treatment. Of the three A. tumefaciens strains tested, EHA101/pIG121Hm was found to be most effective followed by GV3101/pIG121Hm and LBA4404/pIG121Hm for transient GUS expression after 3 days co-cultivation. Inclusion of 100 $\mu$M acetosyringone in both the inoculation and co-cultivation media lead to an improvement in transient GUS expression observed in targeted calli. Vacuum treatment during infection of calli with A. tumefaciens strains increased transformation efficiency. The highest stable transformation efficiency of transgenic plants was obtained when mature seed-derived calli infected with A. tumefaciens EHA101/pIG121Hm in the presence of 100 $\mu$M acetosyringone and vacuum treatment for 30 min. Southern blot analysis indicated integration of the transgene into the genome of tall fescue. The transformation system developed in this study would be useful for Agrobacterium-mediated genetic transformation of tall fescue plants with genes of agronomic importance.

• Journal of Ginseng Culture
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• v.2
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• pp.9-16
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• 2020

Ginseng was first addressed ever in the medical record in HyangYakGooGupBang (鄕藥救急方), the oldest Korean medical book published in Kingdom of Goryeo (918-1392) when Ganghwa was the provisional capital city at the time. It is believed that ginsengs in Ganghwa were planted and cultivated from 1100s. Intensive ginseng production in Ganghwa began when Ganghwa became the special district of the Kaesong Ginseng Union (開城人蔘組合) in 1920s, this intensive production continued till the Korean War in 1950. After the Korean War ended in 1953, ginseng production was resumed. In 1967, Ganghwa Ginseng Association (江華蔘業組合) was founded. The total acreage of ginseng harvested was nearly 200 ha in 1967 and it increased to ha 900 in 1974. By mid-1970s, Ganghwa became the largest ginseng region in Korea by total production and acreage. Most of ginseng roots cultivated in Ganghwa are six years old. Ganghwa, which was already well-known for red ginseng productions, has become even more famous for ginseng production.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.131.2-132
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• 2003

White rot on garlic caused by Sclerotium cepivorum firstly occurred at Goheoung, Jeonnam in 1998. Thereafter, the disease rapidly spread throughout the country except Gangwon and became a major limiting factor for the cultivation of various Allium species such as garlic, onion, and welsh onion. The disease that has not been reported on a wild garlic(Allium monanthum) previously occurred severely at Seosan, Choongnam in 2003. Among cultivation areas in the region, 10.7％ were infected by the disease and the ratio of diseased plant reached up to 55.0％ in some heavily infected fields. Two species of Sclerotium were consistently isolated from infected samples and identified as S. cepivorum or another Sclerotium sp. Averaged size of sclerotium of the former was 455.0x562.2 urn, while the later was 374.4${\times}$347.2$\mu\textrm{m}$. Patogenicity to Allium species and mycological characteristics such as sclerotium size, growth temperature, and microconidia of the fungi were similar to those reported on other Allium species previously. Consequently, the wild garlic is a newly reported host of the two pathogenic fungi in Korea.

• Journal of Bio-Environment Control
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• v.25 no.2
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• pp.95-99
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• 2016

As the survey results of cultivation area, farmhouse, yield and income to clarify the effect of PO Film on Korean melon industry in Seongju region, the covering areas of PE film of Korea, Kyungsangbuk-do and Seongju-gun in 2014 were decreased 91.5%, 94.9% and 95.2% as to 2010. In contrary, the covering areas of PO film of Korea, Kyungsangbuk-do and Seongju-gun in 2014 were increased 342.5%, 500% and 825% as to 2010. The cultivation area and farmhouse in 2015 were decreased 8.8% and 11.9% than those in 2010. The yield and production in 2015 were 38.2% and 25.9% increased han those in 2010. The gross and net income in 2015 were 1.3 and 2.7 times increased than those in 2010. Finally, to change as PO film, the productivity will be increased. And cleaning work and noxious materials discharge will be decreased.

• Korean Journal of Soil Science and Fertilizer
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• v.47 no.6
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• pp.500-505
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• 2014

In recent years, organic ginseng cultivation has increased because customers prefer organic ginseng products due to the morphological quality as well as the safety such as the residuals of chemically-synthesized pesticides. Therefore, some of paddy and upland fields were converted into organic ginseng fields. Soil chemical properties, soil microflora, and soil-inhabiting animals were investigated in paddy-converted and upland organic ginseng fields in Sangju city, Korea. There was few difference in the soil chemical properties, and the soil nutrient concentrations, such as nitrate-N, Av. $P_2O_5$ between the two field types, and exchangeable cations such as K and Ca were within the ranges which are recommended by the standard ginseng-farming manual. Changes in microflora were also assessed by analyzing phospholipid fatty acid composition. Overall, indicators of microbial groups were greater in the upland field than in the paddy-converted soil, but they were not significantly different. In addition, there was no significant change in the abundance of nematodes, collembolans, and mites between the two field types probably because of the high variation within the field types. In this study, it was suggested that soil chemical and biological properties for organic ginseng cultivation were greatly influenced by the variation of topography and soil management practices rather than field types. Further study may be needed to investigate the influence of these factors on soil chemical and biological properties in organic ginseng soils.

• Journal of Microbiology and Biotechnology
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• v.17 no.1
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• pp.81-88
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• 2007

The sprC gene encodes Streptomyces griseus protease C (SGPC), a bacterial chymotrypsin-like serine protease. Because the published data on sprC was not complete, we cloned and analyzed a new DNA fragment spanning downstream to upstream of the sprC gene from S. griseus IFO13350. The cloned 2.3-kb DNA fragment was placed on a high-copy number plasmid and introduced into Streptomyces lividans TK24. Chymotrypsin activity of the transformant was 8.5 times higher than that of the control after 3 days of cultivation and stably maintained until 9 days of cultivation, which dearly indicated that the cloned 2.3-kb fragment contained the entire sprC gene with its own promoter. When the same construct was introduced in the S. griseus IFO13350 (wild strain) and its two mutant strains in the A-factor regulatory cascade, ${\Delta}adpA$ and HO1, the chymotrypsin activity increased fivefold only in the ${\Delta}adpA$ strain. Transcriptional analysis based on RT-PCR revealed that the sprC gene is normally transcribed in both strains; however, earlier transcription was observed in the wild strain compared with the ${\Delta}adpA$ strain. A gel mobility shift assay showed that the AdpA protein did not bind to the promoter region of sprC. All these data clearly indicate that the expression of sprC is not dependent on the AdpA protein, but is distinctly regulated from other chymotrypsin genes composing an AdpA regulon. Earlier morphological differentiation was observed in S. lividans TK24, and S. griseus IFO13350 and HO1, transformed with the expression vector. The transformant of S. griseus ${\Delta}adpA$ formed markedly larger colonies. Antisense repression of sprC resulted in severe decrease of chymotrypsin activity, down to one-third of the control, and delayed morphological differentiation. All these data suggest that SGPC is related to normal morphogenesis in S. griseus.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.30 no.4
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• pp.397-404
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• 2012

Jeju where concentrates on agriculture and tourism, conversion of outdoor culture into cultivation under structure happens actively for the purpose of increasing profit so continuous examination on house cultivation area is very important for this region. This paper is to suggest the effective image classification method using high resolution satellite image to detect the greenhouse. We carried out classification of greenhouse using the supervised classification and rule-based classification method about Formosat-2 images. Connecting result of two classification try to find accuracy improvement for greenhouse detection. Results about each classification method were calculated the accuracy by comparing with the result of visual detection. As a result, mahalanobis distance among the supervised methods was resulted in the highest detection. Also, it could be checked that detection accuracy was improved by tying with result of supervised method and result of rule-based classification. Therefore, it was expected that effective detection of greenhouse would be feasible if henceforward further study is performed in the process of connecting supervised classification and rule-based classification.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1996.06c
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• pp.263-269
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• 1996

In indian agricultural , the energy use pattern has played dominant role in influencing the level of mechanization . Besides that the agro-climatic factors as well as the size of holdings do affect the degree of mechanization . Nearly 30 percent of total cultivated area is owned by l76 percent of the small and tiny land holders each owning even less than a hectare. On the other hand, about 2 percent of land owners cultivate land. These variabilitieshave greatly influenced the ownership of power sources on Indian farms. Small farmers, employ human and animal energies with the use of hand tools and animal drawn equipments. Whereases, the use of tractors, power tillers, electric motors, etc. on small farms is on a marginal scale. There are few pockets and also extensive wheat growing regions where mechanical and electrical power sources are extensively used in production agriculture leading to about 185% of cropping intensity . In that region, the animal energy is employed for on the farm transport of fertilizers, fodders and fuel to support milch animals and other household activities . Inspite of high degree of mechanization, the harvesting of crops is done by human labour with few exceptions of harvesting wheat crops by combines in few pockets. In overall assessment of mechanization, the following conclusions are drawn : ⅰ) Farm operation which show a growing trend of mechanization are (a) tillge, (b) seedling (c) Irrigation (d) Plant protection application (e) Threshing and (f) Transport . ⅱ) Crop cultivation system in respect of wheat, maize and sorghum have been greatly mechanized. ⅲ) The least mechanized cropping systems are (a) vegetable production and (b) cultivation of sugarcane, cotton, rice and pulses. ⅳ) Annual production of tractor has touched the figure of 280.000 by 1995 and the total number has crossed 1.5million on Indian farms.