• Journal of Marine Bioscience and Biotechnology
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• v.10 no.2
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• pp.73-82
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• 2018

Chlorella are source of valuable compounds as lipids, proteins, carbohydrates, bioactive compounds. To continuous obtain the high-value materials of Chlorella protothecoides, we performed continuous cultivation after applying milking techniques to C. protothecoides grown with culture for 7 days in optical panel bioreactor (OPBR) system. Fatty acid and lutein in extracts from pre- and post-milking of C. protothecoides were analyzed using gas chromatography and high performance liquid chromatography, respectively. C. protothecoides were rich in unsaturated fatty acids with a high content of oleic acid(C18:1), which is suitable as a biofuel feedstock. The fatty acid content in pre- and post-milking of C. protothecoides was decreased from 126.424mg/g d.w. to 119.341mg/g d.w, and the lutein content decreased from 0.258mg/g d.w. to 0.178mg/g d.w. The results of this study demonstrate the feasibility of milking C. protothecoides for production of lipids for biofuels production. It was confirmed that microalgae can continuously obtain lutein present in a trace amount through a continuous culture from milking.

• The Plant Pathology Journal
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• v.38 no.5
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• pp.472-481
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• 2022

Brown blotch disease, caused by Pseudomonas tolaasii, is one of the most serious diseases in mushroom cultivation, and its control remains an important issue. This study isolated and evaluated pathogen-specific bacteriophages for the biological control of the disease. In previous studies, 23 varieties of P. tolaasii were isolated from infected mushrooms with disease symptoms and classified into three subtypes, Ptα, Ptβ, and Ptγ, based on their 16S rRNA gene sequences analysis and pathogenic characters. In this study, 42 virulent bacteriophages were isolated against these pathogens and tested for their host range. Some phages could lyse more than two pathogens only within the corresponding subtype, and no phage exhibited a wide host range across different pathogen subtypes. To eliminate all pathogens of the Ptα, Ptβ, and Ptγ subtype, corresponding phages of one, six, and one strains were required, respectively. These phages were able to suppress the disease completely, as confirmed by the field-scale on-farm cultivation experiments. These results suggested that a cocktail of these eight phages is sufficient to control the disease induced by all 23 P. tolaasii pathogens. Additionally, the antibacterial effect of this phage cocktail persisted in the second cycle of mushroom growth on the cultivation bed.

• Korean Journal of Organic Agriculture
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• v.28 no.4
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• pp.555-577
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• 2020

This study analyzes the management performance and productivity of environmentfriendly farms compared to conventional farms and the trend of changes in price premium rates of environment-friendly agricultural products. And environmentfriendly farms in Jeollanam-do are surveyed for difficulties in management, proper premium rate of environment-friendly agricultural products (WTA), and tasks for promoting sales. According to the analysis results, the management performance and productivity of are low in many items, and the number of items that are on the decline or stagnant in the environment-friendly premium is making it difficult for farmers to manage. According to a farm survey, the most important task is to promote school meals for boosting sales of environment-friendly agricultural products. And 65.5% of the respondents having contract cultivation, nearly half or 41.1% of the respondents said they do not need contract cultivation or want contract cultivation for less than one year, which means that the current contract does not meet the needs of farmers. Finally, the environment-friendly premium rate based on consumer prices is generally lower than the premium rate (WTA) that farmers perceive as appropriate, so it is important to resolve the gap between the actual premium rate and the WTA.

• Korean Journal of Agricultural Science
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• v.41 no.4
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• pp.481-485
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• 2014

According to statistical data the past ten years, cultivation area and yield of radish are steadily decreasing. This phenomenon cause instability of radish's supply due to meteorological chage, even if radish's yield per unit area is increasing by cultivation technological development. These problems raise radish's price. So, we conducted study on meteorological factors for accuracy improvement of radish yield estimation. Panel analysis was used with two-way effect model considering group effect and time effect. As the result, we show that mixed effects model (fixed effect: group, random effects: time) was statistical significance. According to the model, a rise of one degree in the average air temperature on August will decrease radish's yield per unit area by $428kg{\cdot}10a^{-1}$ and that in the average air temperature on October will increase radish's yield per unit area by $438kg{\cdot}10a^{-1}$. The reason is that radish's growth will be easily influenced by meteorological condition of a high temperature on August and by meteorological condition of a low temperature on Octoboer.

• Journal of Korean Society of Rural Planning
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• v.20 no.3
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• pp.55-66
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• 2014

Under high agricultural automation in rice farming, cultivation acreage expansion can be considered for the economies of scale. Also, considering the circumstances of higher ratio in leased farms than independent farms, Long-term Leasing Business of Farmland can be the most public and transparent method to expand cultivation acreage for lease farming. Therefore, this research observes the various factors such as application motives, satisfaction, and demands of Long-term Leasing Business of Farmland, which the importance is on the rise and serves as a means to increase agricultural expansion, as well as its operational performance analysis. The research results and implications are as follows. Long-term Leasing Business of Farmland have positive effects such as ensuring stable farming through documental contract, providing convenience because the lease contractor requires no meetings during the contract period, providing opportunities to expand the farm size, and acquiring economical gain which is linked to satisfaction aspects of the businesses. On the other hand, demands such as strengthened business advertisement, writing business documents by correlating with related organizations, and clear lease price configuration were made by the businesses. To bring improvements, this research proposes; 1) expanding support to related businesses, 2) providing convenience through related organization correlations, and 3) configuring clear lease prices considering the regional conditions.

• Korean Journal of Agricultural Science
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• v.48 no.3
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• pp.483-492
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• 2021

This study attempted to estimate the degree of management of oak mushroom farms in Chungcheongnam-do and to provide information to establish oak mushroom cultivation-related policies. The oak mushroom management standard diagnostic table consists of three major categories, growing condition, inoculation management, cultivation management and management administration, along with 20 subcategories. Thus, 209 households of oak mushroom farms were surveyed from 2015 to 2018 in Gongju, Cheongyang, Buyeo and Seochun in Chungcheongnam-do. The average score for the 20 subcategories was 71.5 points (representing a significant level), indicating that these areas have excellent management conditions. The analysis of the management performance indicators revealed a high number of indicators with scores of five or above. The total score was higher, and the amount per bed log and the rate of top-grade products in the total output were also higher, indicating a significant correlation. These findings will provide consulting services to oak mushroom growers as they highlight the correlation between the higher scores of indicators in the oak mushroom management standard diagnostic table and the management performance of farmers. We found that the scores of the indicators for management administration, such as management record and analysis and fund plan were relatively lower than those of other indicators. It is assumed that the owners aging has led to negligence in recording the details on incomes, expenditures, and work and lowered the willingness to make substantial profits. Therefore, it is essential to overcome these problems for profitable oak mushroom farming.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.312-316
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• 2012

Very important factor for crop cultivation are water, nutrient and temperature. However, the essential factor for crop cultivation is water management. Water management is the most important and difficult problems in crop cultivation. The water saving irrigation manual can be used with easy to the farmer and who want automatic irrigation without soil sampling and any kinds of sensors measuring soil water status. The water requirement of red pepper cultivated in plastic film house is different according to soil texture, area as well as climate condition and growth stage. And, the measurement of potential evapo-transpiration (PET) and crop coefficient (Kc) to decide optimum irrigation schedule is very difficult. Results : The average PET during 30 years of northern region of korea for the red pepper cultivation was a $2.31mm\;day^{-1}$. The water saving irrigation manual as water saving is possible, those irrigation interval and amount of irrigation according to growing season and soil texture, are developed using the lysimeter experiments carried out by the RDA for 11 years about potential evapo-transpiration, Kc for the northern region of korea.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.6
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• pp.1259-1267
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• 2015

In Korea, rural groundwater development faces new challenge, which have not been experienced so far. The problem is a groundwater depletion by the water curtain cultivation (WCC) during winter season. This study investigates the groundwater depletion using three-dimensional finite difference groundwater flow program, MODFLOW to verify the water budget of the shallow aquifer of Cheongweon area. Interdisciplinary research, which has become a worldwide trend, has been adopted in studying groundwater modeling in field scale. In particular, the method of groundwater recharge estimation adopted precise modeling techniques, SWAT to groundwater flow modeling. Based on qualified field data, the model calibrated and validated its reliability. The objective of this study is to simulate various stream-aquifer interactions according to groundwater pumping with artificial boundaries, such as weirs and drainage system. We also analyzed a seasonal variation of cumulative water budget of the site to quantify the groundwater depletion and recovery in the pumping field.

• Korean Journal of Medicinal Crop Science
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• v.15 no.2
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• pp.100-104
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• 2007

A protocol for the production of transgenic Panax ginseng C.A. Meyer was established via Agrobacterium tumefaciens-mediated genetic transformation of direct somatic embryos. A number of conditions related to the co-cultivation were tested with respect to maximizing transformation efficiency. The results showed that pH of the co-cultivation medium (5.7), the bacterial growth phase (optical density; $OD_{600}$ = 0.8), co-cultivation period (3 days), and acetosyringone concentration $(100\;{\mu}M)$ had positive effects on transformation. Selected plantlets were cultured on the medium at an elevated hygromycin level(30 mg/l). Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using hpt primers and by Southern hybridization using hpt-specific probe. The transgenic plantlets were obtained after 3-month cultivation and did not show any detectable variation in morphology or growth characteristics compared to wild-type plants.

• Korean Journal of Agricultural Science
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• v.40 no.4
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• pp.281-287
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• 2013

In Korea, isolated cultivation has been implemented for 102 genera, including about 250 species, each of which has underwent microscopic inspection, cultivation of bacteria in selective medium, analysis of physiology and biochemistry, enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). The number of isolated microorganisms was 8,307 in the period of 2005-2012, and bulbs and tubers had the greatest diversity of microorganisms, of 5,165 (62.2%), followed by 2,119 (25.0%) sapling, 796 (9.6%) seed, 150 (1.8%) cutting slip, 70 (0.8%) branch graft and 7 (0.1%). The number of cases which were disqualified were 413 (4.97%), after the detection of 47 disease causing species of microorganism. Viruses predominated, with 27 species, followed by 16 fungi, a viroid, a Chromalveolata and 2 further species. Top on the list of detection was Arabis mosaic virus (77 cases), followed by Tobacco rattle virus (70 cases), Lily symptomless virus (46 cases) and Penicillium expansum (46 cases).