• Title/Summary/Keyword: Cultivated Area

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Simultaneous determination and difference evaluation of 14 ginsenosides in Panax ginseng roots cultivated in different areas and ages by high-performance liquid chromatography coupled with triple quadrupole mass spectrometer in the multiple reaction-monitoring mode combined with multivariate statistical analysis

  • Xiu, Yang;Li, Xue;Sun, Xiuli;Xiao, Dan;Miao, Rui;Zhao, Huanxi;Liu, Shuying
    • Journal of Ginseng Research
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    • v.43 no.4
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    • pp.508-516
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    • 2019
  • Background: Ginsenosides are not only the principal bioactive components but also the important indexes to the quality assessment of Panax ginseng Meyer. Their contents in cultivated ginseng vary with the growth environment and age. The present study aimed at evaluating the significant difference between 36 cultivated ginseng of different cultivation areas and ages based on the simultaneously determined contents of 14 ginsenosides. Methods: A high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometer (MS) method was developed and used in the multiple reaction-monitoring (MRM) mode (HPLC-MRM/MS) for the quantitative analysis of ginsenosides. Multivariate statistical analysis, such as principal component analysis and partial least squares-discriminant analysis, was applied to discriminate ginseng samples of various cultivation areas and ages and to discover the differentially accumulated ginsenoside markers. Results: The developed HPLC-MRM/MS method was validated to be precise, accurate, stable, sensitive, and repeatable for the simultaneous determination of 14 ginsenosides. It was found that the 3- and 5-yr-old ginseng samples were differentiated distinctly by all means of multivariate statistical analysis, whereas the 4-yr-old samples exhibited similarity to either 3- or 5-yr-old samples in the contents of ginsenosides. Among the 14 detected ginsenosides, Rg1, Rb1, Rb2, Rc, 20(S)-Rf, 20(S)-Rh1, and Rb3 were identified as potential markers for the differentiation of cultivation ages. In addition, the 5-yr-old samples were able to be classified in cultivation area based on the contents of ginsenosides, whereas the 3- and 4-yr-old samples showed little differences in cultivation area. Conclusion: This study demonstrated that the HPLC-MRM/MS method combined with multivariate statistical analysis provides deep insight into the accumulation characteristics of ginsenosides and could be used to differentiate ginseng that are cultivated in different areas and ages.

Research on the Ground Water Developement in the Region of Choong Nam Province (충남지역의 지하수개발에 관한 조사)

  • 민병섭
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.11 no.4
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    • pp.1827-1831
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    • 1969
  • Resulties of research on the capacity of ground water of 994 concrete-pipe-wells and 97 infiltration-gallerys in ground-water-developement-works region executed from March to Julyin 1969, in Choong Chung Nam Do, and research on the quality of ground water for 88 wells for home-use around of River Geum Area, are as fellows: (1) Thickness of aquifer is no more than 2.85m averagely even at river-overflowed plain, alluvial plain and valley plain area that are estimated to contain ground water mostly. And so, it is guessed that ground water capacity is not much especially. (2) Soil of aquifer of the above area is sand or gravel and it is estimated to be good for ground water developement and its mean permeability coefficient is bout $2.5{\times}10^{-3}$(m/sec), and its porosity is about 33.9%. (3) The quality of ground water is good for irrigation water exception of delta plain area. Warm water plan is to need for irrigation water when water temperature is less than 19 degrees below zero. (4) Prospect of ground water developement, judging from quality and quantity, expects to lay infiltration gallery under the ground at river bed in order to utilize under-flow-water of river bed, river-overflowed plain, alluvial plain and valley plain that ground level is less than 50m. (5) Collectable water volume of under-flow-water of river bed is about 450 to $750m^3/day$ to be able to irrigate 3ha to 5ha of the cultivated land in case that infiltration gallery length is 50m and its depth is about 5m. (6) Collectable water volume at river-overflowed plain, alluvial plain and valley plain area, is estimated $150m^3/day$ to be able to irrigated 1ha of the cultivated land.

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Investigation of some Forms of Nitrogen in burley Tobacco Leaf Cultivated in Mokpo, Namwon and Yaesan District. (목포, 남원, 예산지방 Burley 엽중 형별질소의 경향 조사 연구)

  • 김신일;김찬호
    • Journal of the Korean Society of Tobacco Science
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    • v.1 no.2
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    • pp.120-126
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    • 1979
  • The nitrogen contents of some forms in Burley tobacco leaf cultivated in Yaesan, Mokpo and Namwon district were investigated. The rate of each form in total nitrogen contents were as follows ; Protein form nitrogen 30~33 % Nitrate form nitrogen 10~123% Alkaloid form nitrogen 8~16 % Ammonia form nitrogen 6~9% Amide form nitrogen 2~ 3% Other form nitrogen 26~44 % The order of nitrate form nitrogen content on the nitrogen of each tobacco cultivated in three area was Yaesan > Namwon > Mokpo, but that of alkaloid form nitrogen was reverse order of nitrate form nitrogen. As for Quality ( grade ), the orders of alkaloid and ammonia form nitrogen content on total nitrogen were H5> 3> 1> L 1> 3> 5, but that of nitrate form nitrogen was reversed.

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Vesicular-arbuscular mycorrhizal Fungi found at the horticultural and cultivated Plants (원예식물 및 재배식물에서 발견된 내생균근)

  • Lee, Sang-Sun;Ka, Kang-Hyeon;Lee, Sog-Koo;Paek, Kee-Yoeup
    • The Korean Journal of Mycology
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    • v.19 no.3
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    • pp.186-202
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    • 1991
  • Out of the 36 species (22 families) of horticultural plants collected from the horticultural shop around Cheong Ju, the 17 plant species (47.2%) were infected with VA-mycorrhizae in the root tissues. Also, the chlamydospores or azygospores of VA-mycorrhizae were identified (two genera, three species); Acaulospora spinosa, Glomus etinucatum, and G. tortusom. VA-mycorhizae found from the cultivated plants around Korea National University of Education and other area were also identified (four genera, six species); A. myriocarpa, Gigaspora decipiens, G. caledonium, G. glomerulatum, G. microcarpum, and Scutellospora calospora.

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Habitat Types of Wintering Season Wildbirds Depending on Land Use, Hanam (하남시 토지이용현황에 따른 겨울철 야생조류 서식유형 분석 연구)

  • Kim, Jeong-Ho
    • Journal of Environmental Science International
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    • v.15 no.11
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    • pp.1077-1086
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    • 2006
  • This study aims at analyzing relationships between land use and habitat types of winter wildbirds to provide basic understanding of ecosystem for preservation and restoration of urban ecosystem in the future. The research area is Hanam City. Researches on land use types showed Hanam City had 79.1% of greenspace and openspace, but intensive urban development has been taking place in greenspace that is adjacent to urban districts. This has brought the problems of lack of greenspace in urban districts and damages to cultivated areas and grassland. A total of 61 and 8,642 populations of winter wildbird species were observed in research areas. Paradoxornis webbiann(16.91), Passer montanus(11.93), Pica pica(6.88) were dominant species. When they were divided according to habitat types, 20 species of interior species, 8 species of interior-edge generalist species, 12 species of edge species and 3 species of urban species were observed. When which land use type was mostly served as wildbirds habitats was examined, urban species(3 species and 290 populations) was a dominant species in urban districts while in greenspace and openspaece, water species(19species and 3,075 populations) including winter migratory birds was. Among greenspace and openspaece, edge species was dominant in forest while urban species was a dominant species in cultivated areas. This shows there is a need to improve diversity of wildbirds through restoration of cultivated areas in the central part of Hanam City.

Endophytic Trichoderma citrinoviride isolated from mountain-cultivated ginseng (Panax ginseng) has great potential as a biocontrol agent against ginseng pathogens

  • Park, Young-Hwan;Mishra, Ratnesh Chandra;Yoon, Sunkyung;Kim, Hoki;Park, Changho;Seo, Sang-Tae;Bae, Hanhong
    • Journal of Ginseng Research
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    • v.43 no.3
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    • pp.408-420
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    • 2019
  • Background: Ginseng (Panax ginseng Meyer) is an invaluable medicinal plant containing various bioactive metabolites (e.g., ginsenosides). Owing to its long cultivation period, ginseng is vulnerable to various biotic constraints. Biological control using endophytes is an important alternative to chemical control. Methods: In this study, endophytic Trichoderma citrinoviride PG87, isolated from mountain-cultivated ginseng, was evaluated for biocontrol activity against six major ginseng pathogens. T. citrinoviride exhibited antagonistic activity with mycoparasitism against all ginseng pathogens, with high endo-1,4-${\beta}$-D-glucanase activity. Results: T. citrinoviride inoculation significantly reduced the disease symptoms caused by Botrytis cinerea and Cylindrocarpon destructans and induced ginsenoside biosynthesis in ginseng plants. T. citrinoviride was formulated as dustable powder and granules. The formulated agents also exhibited significant biocontrol activity and induced ginsenosides production in the controlled environment and mountain area. Conclusion: Our results revealed that T. citrinoviride has great potential as a biological control agent and elicitor of ginsenoside production.

Change of Soil Chemical Properties according to Cultivation Area and Cultural Year for Platycodon grandiflorum (도라지 재배지역 및 재배년수에 따른 토양의 화학적 특성변화)

  • Lee, Cheol-Ho;Lee, Shin-Woo;Lee, Hyub
    • Korean Journal of Medicinal Crop Science
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    • v.18 no.4
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    • pp.273-279
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    • 2010
  • The study result survey platycodon cultivation regions of Jinju(2), Sacheon(4), Hapcheon(3), Haman(3), and Sancheong(3) of Gyongnam regarding chemical properties of soil, inorganic nitrogen phosphorus by type of composition is as follows: pH and EC value were highest where platycodon cultivated for under 3 years in soils. Content of O.M equal to or less than 25 mg $kg^{-1}$, the average value for platycodoncultivation in korea. Content of exchangeable calcium in soils were 12, 14 $cmol^+kg^{-1}$ in Hapcheon and Haman regions where platycodon was cultivated for two years which is 2~3 time higher level compared with the average upland soil of korea. Water solution boron confirmed approximated level of 3.0 mg $kg^{-1}$ in all survey regions, and the finding is about five times higher than average upland soil of korea which is 0.5 mg $kg^{-1}$. In the case of nitrogen, a significant difference was found depending on cultivation regions and continuous culture. Content of nitrogen in soils were difference according to cultivation area and continuous culture. The composition ratio according to the type of inorganic phosphorus showed the highest in order of Al-P > Ca-P > Fe-P > Saloid-P in all survey regions except for Hapcheon (five, seven years cultivated soils). The correlation showed high significance between available phosphate and inorganic phosphorus.

Current Status and Improvement Strategies for Landscape Plant Production and Demand -A Case Study of Gangwon State- (조경식물의 생산 및 수요 현황과 개선 방안 -강원특별자치도를 사례로-)

  • Min-Jung Choi;Gab-Soo Han;Kwang-Min Ham
    • Journal of Environmental Science International
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    • v.32 no.12
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    • pp.915-923
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    • 2023
  • The purpose of the study is to explore strategies to revitalize the distribution of landscaping plants in Gangwon State (hereinafter referred to as Gangwon-do). To understand the current distribution status and perception of landscape materials in Gangwon-do, a survey was conducted on producers and consumers focusing on cultivation area and method, cultivated species and quantity, purchase species and quantity, and purchase area. Producers often cultivated open land, and in the case of trees and shrubs, most of them cultivated <5 species. On the other hand, consumers preferred field cultivation products when purchasing trees and shrubs. Approximately 6-10 species of trees and shrubs were preferred for a single purchase, confirming an imbalance in the supply and demand of landscaping plants. In addition, both producers and consumers had a positive perception of landscape plant transactions in Gangwon-do; however, the dissatisfaction factors for producers included a small consumer base and difficulties for them in securing standards and quantities. Based on these results, it is necessary to establish a platform that can interconnect producers' landscape plant cultivation information with consumers' requirements to rejuvenate the landscape plant distribution market and enhance competitiveness in Gangwon-do. In addition, this platform is expected to have a positive impact on improving the quality of landscaping plants, setting reasonable prices, and increasing domestic demand in Gangwon-do by providing opportunities for cultivation, promotion, and marketing education through producer support projects.

A Study on Major Local Foods in Gwangju.Jeonam Area (광주.전남지역의 주요 특산식품에 대한 연구)

  • Kim, Jeong-Ok;Choi, Cha-Ran;Shin, Mal-Shick
    • Korean Journal of Human Ecology
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    • v.15 no.2
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    • pp.327-339
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    • 2006
  • We have investigated the distribution of major local foods cultivated in Gwangju City and Jeollanam-Do in order to understand the food culture of Honam region. The local foods were divided into 4 classes: agricultural, aquatic, animal and other products. 124 species of local foods, including 51 agricultural products, 50 aquatic products, 6 animal products and 17 others, were identified from this region. In Jeollanam-Do area, the agricultural products have a variety of fruits and vegetables, and aquatic products have fishes. In particular, rice, cucumber, pear, anchovy, oyster, laver, oyster mushroom, shiitake mushroom, and honey were determined as major local foods in Jeollanam-Do area. Note that Mudeungsan watermelon is cultivated in Gwangju, and sweet smelt and cat fish caught only in Sumjin river and Tamjin river area. In recent years, pumpkin (Cucurbita maxima Duchesne), paprika, kiwi and melon were determined as major local foods in Jeollanam-Do. Furthermore, branding strategy of agricultural and animal products can be said to improve. Wild types of aquatic productions, mushrooms and wild edible greens have been decreasing, whereas cultivated production increasing. The kinds of special local foods in Gwangju City and Jeollanamdo area were influenced by geographical, religious and social factors.

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Growth Characteristics and Ginsenoside contents of Korean Ginseng (Panax ginseng C.A. Meyer) by Green Manure Crops (녹비작물 재배에 따른 인삼의 생육과 진세노사이드 함량)

  • Seong, Bong-Jae;Han, Seong-Ho;Kim, Sun-Ick;Kim, Gwan-Hou;Lee, Ka-Soon;Kim, Hyun-Ho;Won, Jun-Yeon;So, Jung D.;Cho, Jin-Woong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.3
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    • pp.364-368
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    • 2014
  • This study investigated ginseng growth and ginsenoside contents after control a reserved ginseng cultivation land using various green manure crops for stable ginseng cultivation. Followings are results obtained from this research. After cultivate the green manure crops, microbial flora in soil was diversified, organic matter and total-N content increased, but salt content decreased. The highest output obtained from the wheat cultivated area among various green manure corps. Growth of shoot and root of two years old ginseng increased significantly at the green manure crop cultivated area. Specially, the wheat cultivated area was the most effective in growth. Also, the rate of the leaf discoloration at the aerial part and the rusty root at the root was the least at the wheat cultivation area. Meanwhile, the ginsenoside content was the most at the wheat cultivation area. Thus, the reserved ginseng cultivation land could be managed by cultivating wheat for effective ginseng growth.