• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.329-329
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.335-335
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• 2005

• The Plant Pathology Journal
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• v.17 no.5
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• pp.243-248
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• 2001

Two Tobamoviruses, Cucumber green mottle mosaic virus (CGMMV) and Zucchini green mottle mosaic virus (ZGMMV), occurred in Korea in 463 ha in 1998, 33.9 ha in 1999, and 44.2 ha in 2000. CGMMV was detected in watermelon, cucumber, oriental melon, and melon, whereas ZGMMV was mainly detected in zucchini squash. Thirty-six CGMMV isolates wee classified into three types by analysis of single strand cDNA conformational polymorphism (SSCP) of the coat protein gene. In a comparison of serological relationships among CGMMV, ZGMMV, and Kyuri green mottle mosaic virus (KGMMV), the three tobamoviruses specifically reacted with each homologous antibody in the double-antibody sandwich enzyme-linked immunosorbent assay and rapid imunofilter paper assay (RIPA), although ZGMMV and KGMMV were slightly biologcially similar. In a survey of the three tobamoviruses in cucurbitgrowing field in Korea by RIPA, CGMMV and ZGMMV were detected but KGMMV was not found in commercially growing cucurbit crops so far. Seed contamination ratio of CGMMV in bottle gourd seeds tested was 84%, while seed trasmission ratio from the virus-contaminated seeds was 2.0%. Soil transmission ratio was 0-3.5% in fields naturally infested with CGMMV or ZGMMV. Control measures of the virus diseases are roguing and sanitation. These suggest that it is important to rogue the first infected crops, which include the seed and soil, especially early in the season. This may be practicable to control the diseases because CGMMV and ZGMMV have a narrow host range restricted to cucurbitaceous crops.

• Horticultural Science & Technology
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• v.32 no.1
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• pp.26-32
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• 2014

We investigate the effect of post-conditioning periods and seed orientation on the vigor of cucurbit seeds with dry heat treatment (DHT). All the dry-heat treated seeds exhibited varying degree of seed vigor decreases. In general, pumpkin seeds showed less vigor decreases than the bottle gourd seeds. When the dry heat treated seeds were germinated after post-conditioning for 0, 30, and 120 days, the percentage of germination was enhanced by increasing the period of post-conditioning and the efficiency of post-conditioning differed by crop and cultivar. In both bottle gourd and pumpkin, the vigor of seeds placed in vertically upward and horizontal orientations was higher than that of the seeds placed in the vertically downward orientation. The results suggested that the vigor of dry-heat treated seeds could be improved by applying the proper post-conditioning and seed orientation.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.276-276
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• 2014

• Research in Plant Disease
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• v.13 no.2
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• pp.82-87
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• 2007

The genetic diagnostic method of virion capture (VC)/RT-PCR was developed for the simultaneous detection of three rod shaped viruses of Cucumber green mottle mosaic virus (CGMMV), Kyuri green mottle mosaic virus(KGMMV) and Zucchini green mottle mosaic virus (ZGMMV) transmitted by seed in Cucurbit. Out of 12 primer combinations for the three tobamoviruses, a primer set of CGMMV-C724, KGMMV-K513 and ZGMMV-Z407A was useful for mono and triplex VC/RT-PCR. The triplex VC/RT-PCR for the three tobamovirus in Cucurbit could detect specifically without interference among primers and/or plant species of watermelon, gourd, cucumber, melon, pumpkin, squash and Nicotiana benthamiana.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.401-408
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• 2015

Horticultural traits and genetic relationship were evaluated for 83 melon (Cucumis melo L.) cultivars. Survey of a total of 36 characteristics for seedling, leaf, stem, flower, fruit, and seed and subsequent multiple analysis of variance (MANOVA) were conducted. Principal component analysis (PCA) showed that 8 principle components including fruit weight, fruit length, fruit diameter, cotyledon length, seed diameter, and seed length accounted for 76.3% of the total variance. Cluster analysis of the 83 melon cultivars using average linkage method resulted in 5 clusters at coefficient of 0.7. Cluster I consisted of cultivars with high values for fruit-related traits, Cluster II for soluble solid content, and Cluster V for high ripening rate. Genotyping of the 83 cultivars was conducted using 15 expressed-sequence tagged-simple sequence repeat (EST-SSR) from the Cucurbit Genomics Initiative (ICuGI) database. Analysis of genetic relatedness by UPGMA resulted in 6 clusters. Mantel test indicated that correlation between morphological and genetic distance was very low (r = -0.11).

• Research in Plant Disease
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• v.25 no.4
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• pp.179-187
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• 2019

This study was carried out to test the antimicrobial activities of nano metal hybrid materials produced by plasma technologies (radio frequency-thermal plasma system and direct current sputtering system) against microbes isolated from cucurbit (watermelon, pumpkin, and gourd) seeds. Eight different nano metal hybrid materials and four carriers were tested against five different fungal and ten different bacterial isolates in vitro. Among the tested nano metal hybrid material, Brass/CaCO₃ (1,000 ppm) exhibited 100% antimicrobial effect against all the five tested fungi. However, nano metal hybrid material Brass/CaCO₃ (1,000 ppm) inhibited only four bacterial isolates, Weissella sp., Rhodotorula mucilaginosa, Burkholderia sp., and Enterococcus sp. at 100% level, and did not inhibited other six bacterial isolates. Nano metal hybrid material graphite-nickel (G-Ni) showed 100% inhibition rate against Rhizopus stolonifer and 52.94-71.76% inhibition rate against four different fungal isolates. Nano metal hybrid material G-Ni did not show any inhibition effects against tested ten bacterial isolates. In summary, among the tested eight different nano metal hybrid materials and four carriers, Brass/CaCO₃ showed inhibition effects against five fungal isolates and four bacterial isolates, and G-Ni showed variable inhibition effects (52.94-100%) against five fungal isolates and did not show any inhibition effects against all the bacterial isolates.

• The Plant Pathology Journal
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• v.39 no.3
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• pp.235-244
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• 2023

Acidovorax citrulli (Ac) is a phytopathogenic bacterium that causes bacterial fruit blotch (BFB) in cucurbit crops, including watermelon. However, there are no effective methods to control this disease. YggS family pyridoxal phosphate-dependent enzyme acts as a coenzyme in all transamination reactions, but its function in Ac is poorly understood. Therefore, this study uses proteomic and phenotypic analyses to characterize the functions. The Ac strain lacking the YggS family pyridoxal phosphate-dependent enzyme, AcΔyppAc(EV), virulence was wholly eradicated in geminated seed inoculation and leaf infiltration. AcΔyppAc(EV) propagation was inhibited when exposed to L-homoserine but not pyridoxine. Wild-type and mutant growth were comparable in the liquid media but not in the solid media in the minimal condition. The comparative proteomic analysis revealed that YppAc is primarily involved in cell motility and wall/membrane/envelop biogenesis. In addition, AcΔyppAc(EV) reduced biofilm formation and twitching halo production, indicating that YppAc is involved in various cellular mechanisms and possesses pleiotropic effects. Therefore, this identified protein is a potential target for developing an efficient anti-virulence reagent to control BFB.

• Journal of Microbiology and Biotechnology
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• v.25 no.9
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• pp.1401-1409
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• 2015

The aim of this study was to develop a SYBR Green-based real-time PCR assay for the rapid, specific, and sensitive detection of Acidovorax avenae subsp. citrulli, which causes bacterial fruit blotch (BFB), a serious disease of cucurbit plants. The molecular and serological methods currently available for the detection of this pathogen are insufficiently sensitive and specific. Thus, a novel SYBR Green-based real-time PCR assay targeting the YD-repeat protein gene of A. avenae subsp. citrulli was developed. The specificity of the primer set was evaluated using DNA purified from 6 isolates of A. avenae subsp. citrulli, 7 other Acidovorax species, and 22 of non-targeted strains, including pathogens and non-pathogens. The AC158F/R primer set amplified a single band of the expected size from genomic DNA obtained from the A. avenae subsp. citrulli strains but not from the genomic DNA of other Acidovorax species, including that of other bacterial genera. Using this assay, it was possible to detect at least one genomeequivalents of the cloned amplified target DNA using 5 × 10⁰ fg/µl of purified genomic DNA per reaction or using a calibrated cell suspension, with 6.5 colony-forming units per reaction being employed. In addition, this assay is a highly sensitive and reliable method for identifying and quantifying the target pathogen in infected samples that does not require DNA extraction. Therefore, we suggest that this approach is suitable for the rapid and efficient diagnosis of A. avenae subsp. citrulli contaminations of seed lots and plants.