• Title/Summary/Keyword: Cryptococcus sp. 9-D-1

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Two New Species of Cryptococcus sp. and Candida sp. from Wild Flowers in Korea

  • Min, Jin-Hong;Kang, Min-Gu;Ryu, Jin-Ju;Lee, Hyang-Burm;Kim, Chang-Mu;Kim, Ha-Kun;Lee, Jong-Soo
    • Mycobiology
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    • v.40 no.4
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    • pp.255-257
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    • 2012
  • Among 80 types of yeast isolated from wild flowers in Daejeon, Korea, two species that have not yet been identified by phylogenetic analysis of the internal transcribed spacer-2 (ITS2) genes and 26S rDNA sequences were identified as Candida sp. 44-C-1 and Cryptococcus sp. 9-D-1. Neither of the newly identified species formed ascospores, while Candida sp. 44-C-1 formed pseudomycelium and Cryptococcus sp. 9-D-1 did not.

Isolation and Identification of Yeasts from Wild Flowers in Gyejoksan, Oseosan and Beakamsan of Korea (대전 계족산과 충남 오서산 및 전북 백암산 주위 야생화들로부터 효모의 분리 및 동정)

  • Min, Jin-Hong;Ryu, Jin-Ju;Kim, Ha-Kun;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.41 no.1
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    • pp.47-51
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    • 2013
  • Yeasts isolated from wild flowers of Gyejoksan in Daejeon city, Oseosan in Chungchungnamdo, and Baekamsan in Jeollabukdo, Korea were identified by comparison of nucleotide sequences for PCR-amplified D1/D2 region of 26S rDNA or internal transcribed spacer (ITS) 1 and 2 including 5.8S rDNA using BLAST. Twelve yeast strains of ten species and seventeen yeast strains of ten species were isolated from wild flowers of Gyejoksan and Oseosan, respectively. And thirty seven yeast strains of twenty four species were isolated from wild flowers of Baekamsan. Total thirty four yeast species were isolated from three different sample collection areas, but only nine species were overlapped from the at least two different sampling areas: Cryptococcus sp., Cryptococcus aureus, Cryptococcus flavescens, Cryptococcus flavus, Metschnikowia sp., Pseudozyma aphidis, Rhodotorula glutinis, Sporobolomyces carnicolor, and Sporobolomyces ruberrimus. Among them only Cryptococcus aureus was occurred from all three different collection sites. Other twenty five species were restricted to specific collection site suggesting that each area has distinctive yeast flora.

Isolation and Identification of Yeasts from Wild Flowers in Deogyu Mountain and their Physiological Functionalities (전라북도 덕유산 일대의 야생화에서 효모의 분리 동정 및 생리기능성)

  • Han, Sang-Min;Hyun, Se-Hee;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.43 no.1
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    • pp.47-52
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    • 2015
  • Several kinds of yeasts were isolated from wild flowers collected from Muju resort of Deogyu mountain in Korea and identified by comparison of nucleotide sequences of D1/D2 region of 26S rDNA using BLAST. Totally twenty-two strains of eight species were isolated and Cryptococcus magnus (9 strains) and Cryptococcus flavescenes (6 strains) were dominant species. The supernatant and cell-free extracts were prepared and their antioxidant activity, anti-gout xanthine oxidase inhibitory activity and whitening tyrosinase inhibitory activity were investigated. Anti-gout xanthine oxidase inhibitory activities of Sporobolomyces phaffii JS00583 and Rhodotorula graminis JS00581 were 40.4% and 39.1%, respectively and also was 36.1% whitening tyrosinase inhibitory activity in the supernatant of Cryptococcus magnus JS00570.

Isolation and Identification of Wild Yeasts from Soils of an Herb Park in Seoul Metropolitan City and Characteristics of Unrecorded Yeasts (서울특별시 허브공원 토양으로부터 야생 효모의 분리, 동정 및 미기록 효모의 특성)

  • Han, Sang-Min;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.44 no.2
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    • pp.108-112
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    • 2016
  • This study focused on the isolation of wild yeasts from the soils of an herb park in Seoul, Korea, and characterization of unrecorded yeasts. Fifteen yeast strains of nine species were isolated and identified. Among them, strains of the Cryptococcus genus, such as Cryptococcus laurentii (2 strains), Cryptococcus saitoi (two strains), Cryptococcus podzolicus (2 strains), and Cryptococcus sp. (2 strains) were dominant. Candida boleticola SU14-2, Candida novakii SU6-2 and Cryptococcus podzolicus SU18-3 were also screened as newly recorded yeasts in Korea. Candida novakii SU6-2 formed ascospores and a pseudomycelium, and Candida boleticola SU14-2 grew well in the 5% NaCl-containing yeast extract-peptone-dextrose medium.