• Journal of Life Science
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• v.27 no.3
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• pp.339-345
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• 2017

Carotenoids are natural lipid-soluble pigments, which are produced primarily by bacteria, algae, and plants. Many studies have focused on the identification, production, and utilization of natural sources of astaxanthin from algae, yeast, and crustacean byproducts as an alternative to the synthetic pigment, which is mostly used today. The aim of the present study was to identify a mutant of Paracoccus haeundaensis by exposure to UV and ethyl methanesulfonate (EMS). The mutant was then exposed to nutrient stress conditions to isolate an astaxanthin-hyperproducing strain, followed by characterization of the mutant. The survival rate decreased in accordance with an increase in the UV exposure time and an increase in the EMS concentration. A mutant of the original P. haeundaensis strain was identified that showed hyperproduction of astaxanthin following exposure to UV irradiation (20 min) and EMS treatment (0.4 M concentration). The optimal culture conditions for the PUE mutant were $25^{\circ}C$, pH 7-8, and 3% NaCl. The effects of various carbon and nitrogen sources on the growth and astaxanthin production of PUE were examined. The addition of 1% raffinose and 3% potassium nitrate influenced cell growth and astaxanthin production. The selected mutant exhibited an increase of 1.58 folds in astaxanthin content compared to initial wild type strain. A genetically stable mutant strain obtained using mutagen (UV irradiation and EMS treatment) may be a suitable candidate for further industrial scale production of astaxanthin.

• Journal of Life Science
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• v.27 no.6
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• pp.726-737
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• 2017

Carotenoids are isoprenoids with a long polyene chain containing 3 to 15 conjugated double bonds, which determines their absorption spectrum. They typically consist of a $C_{40}$ hydrocarbon backbone often modified by different oxygen-containing functional groups, to yield cyclic or acyclic xanthophylls. Much work has also been focused on the identification, production, and utilization of natural sources of carotenoid (plants, microorganisms and crustacean by-products) as an alternative to the synthetic pigment which currently covers most of the world markets. Nevertheless, only a few carotenoids (${\beta}-carotene$, lycopene, astaxanthin, canthaxanthin, and lutein) can be produced commercially by fermentation or isolation from the small number of abundant natural sources. The market and demand for carotenoids is anticipated to increase dramatically with the discovery that carotenoids exhibit significant anti-carcinogenic activities and play an important role in the prevention of chronic diseases. The increasing importance of carotenoids in the feed, nutraceutical food and pharmaceutical markets has renewed by efforts to find ways of producing additional carotenoid structures in useful quantities. Because microorganisms and plants synthesize hundreds of different complex chemical carotenoid structures and a number of carotenoid biosynthetic pathways have been elucidated on a molecular level, metabolic and genetic engineering of microorganisms can provide a means towards economic production of carotenoid structures that are otherwise inaccessible. The aim of this article is to review our current understanding of carotenoid formation, to explain the perceived benefits of carotenoid in the diet and review the efforts that have been made to increase carotenoid in certain microorganisms.

• Journal of agricultural medicine and community health
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• v.39 no.1
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• pp.14-24
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• 2014

Objectives: The purpose of this study was to estimate the prevalence of food allergies and find out food allergens. Methods: Questionnaires were completed by 2,415 subjects in D city, among whom 452 subjects believed that they had food allergy. Skin prick tests were performed on these subjects. The data were analyzed using SAS Version 9.0 and ${\chi}^2$-test. In addition, several recent studies were reviewed. Results: The prevalence rate of food allergic symptoms was 25.6% in males and 25.8% in females, respectively. The positive rate of skin prick tests among those who were allergic to any of 14 food items was 21.4% in males and 14.7% in females(p=0.018). Therefore, it was estimated that the prevalence rate of food allergy was 5.0% in males and 3.6% in females. It was shown that the major food allergens were hop, shrimp, lobster, oyster and egg(whole) in males, and egg(whole), hop, oyster, and pork in females, respectively. The prevalence in recent studies ranged from 1 to 35%. Major food allergens were milk, egg, nuts, fish, shellfish crustacean and fruits/vegetables. Conclusions: In order to provide effective care for food allergy, it is essential to find out the prevalence of food allergies and food allergens.

• The Korean Journal of Pesticide Science
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• v.10 no.3
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• pp.189-195
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• 2006

The purpose of the current study was to develop a standard method to assess the ecological effect of pesticides using the representative Korean freshwater cladocerans. The indigenous cladocerans, Daphnia obtusa, Daphnia sp., Moina macrocopa, Simocephalus vetulus and Daphnia magna formally endorsed by the major international organizations were tested to select optimal culture media in laboratory condition. All of the Korean water flea tested showed good growth and fecundity in the moderately hard water ($CaCO_3$ ranged 80 to 100 mg $L^{-1}$) and soft water ($CaCO_3$ ranged 40 to 48 mg $L^{-1}$). However, considering that the total hardness of fresh water collected from 5 rivers ranged from 60.2 to 127.2 mg $L^{-1}$ ($CaCO_3$) and Ca deficiency could be a major determinant of species success and community structure among crustacean zooplankton, the present study suggests that the optimal water hardness for growing Daphnia sp., Daphnia obtusa, Moina macrocopa and Simocephalus vetulus is $80{\sim}100$ mg $L^{-1}$ of $CaCO_3$.

• Toxicological Research
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• v.22 no.4
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• pp.381-390
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• 2006

Astaxanthine is a pigment that belongs to the family of the xanthophylls, the oxygenated derivatives of carotenoids whose synthesis in plants derives from lycopene. Astaxanthine is also a carotenoid widely used in salmonid and crustacean aquaculture to provide the pink color characteristic of that. Recent study reported that astaxanthine has the role as a detoxicant against the free radicals. On our study, we estimated the genotoxicity in ICR mice and possibility as antioxidant reagents of mutant Phaffia rhodozyma strain over expressing the astaxanthine by gamma-lay and carophyll pink including astaxanthine in apoE knock out mice, respectively. In our study, we administered Phaffia rhodozyma (2 mg and 3 mg) and carophyll pink for 4 and 8 week. The clinical sign and mortality were not detected compared with control groups. In the mutant frequency of hprt gene and chromosome aberration in splenic cells, there was not detected abnormality. There was not critical change in hematological and serum biochemical test compared to control. In expression level of repair enzyme, increase of catalase were detected and increase of expression level of Nrf-2 was detected in Phaffia rhodozyma (3 mg) and carophyll pink in 8 week treated group. In GSH level, the group of treated with Phaffia rhodozyma (3 mg) showed the increase of the GSH. In conclusion, mutant Phaffia rhodozyma and caphyll pink may be applied to the effective food additives to reduce the free radical.

• Animal Systematics, Evolution and Diversity
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• v.7 no.1
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• pp.151-176
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• 1991

The arthropods are the most diverse and successful animal group making up more than three quarters of the total animal species. They also live in a great diversity of habitats on the earth. Because of these unavoidable circumstances, the human life has always been affected by them. Therefore, in many countries from the past, governments have supported the studies of the arthropods on the basis of the national policy. However, the present situation of ours is far behind the other advanced contries, Now we urgently need governmental support fro the development of both the basic and the applied fields of arthropod studies in the well organized manner. Facing with this necessity, the present article reviewed the studies of Korean arthopods based on insect and crustacean representing most of the arthopods. Several subjects were reviewed in the section of the past and present studies on Korean arthropods. These are the studies on Korean arthropods in the past and present, their application to the national strategy, and the present condition of curating specimens, etc. From these reviews, several important points were suggested in the section of future plan. These are such subjects as the establishment of national institution for the curation of specimens , computerizing the data, diversification of research techniques, valid strategy concerning the control of harmful contribution to the nature conversation and environmental management , use as a research animal, the enhancement of public welfare, training of specialists, and policies for the national programs.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.2 no.1
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• pp.31-41
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• 1997

This study was carried out to investigate the composition and the distribution of the benthic community in the coastal area of Inchon seasonally from May 1994 to February 1995, and to estimate the environmental factors that effect the temporal and the spatial changes of benthic species composition. In the present study, 231 species were collected with the average density of 455 ind./$m^2$. The dominant species were Heteromastus sp., Sternaspis scutata, Chaetozone setosa, Mediomastus sp., Glycinde sp., Glycera sp. and Nephtys oligobranchia, which are in the polychaete group; abundance of these species showed seasonal and spatial variations. The study area was divided into 4 regions by cluster analysis such as outside area of Palmido (I), main channel region and adjacent are of Shihwa sea wall (II), north-eastern area of Youngjongdo (III), and the Inchon harbor and the mouth area of Sorae harbor (IV). The particle size of sediment which generally influences the distribution of the benthic community, the differences of the concentration of heavy metals, and of the dissolved oxygen, the increase of the proportion of polychaetes and molluscs, the absence of the crustacean phoxocephalids, and the increase of the abundance of pollution indicator species, indicated that the environment of the areas have been degrading gradually from Group I to Group IV.

• Korean Journal of Environmental Biology
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• v.17 no.2
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• pp.159-168
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• 1999

The ophemeropterans such as Epeorus pellucidus, Uracanthella rufa and Drunella uculea were dominated in station 1. It was generally showed the stable aquatic communities with low dominance index values throughout the year in station 2. The first dominant species in station 3 were all mayflies with the average DI value of 0.38. The first dominant species of Uracanthella rufa known as relatively tolerant species in station 4. A crustacean species Gammarus spp. have frequently occurred as the 1st or 2nd dominant species in station 5. Limnodrilus socialis, and Chironomus spp. were dominated in stations 6 and 7 Station 1, 2 and 3 were classified as oligosaprobic status according to their average diversity index values ranging from 3.46 to 3.52. Station 7 showed $\alpha$-mesosaprobic water condition with the overall average diversity value of 1.26. In general, the equitability and species richness values in the same sampling areas had close relations with their diversity values. The dendrogram drawn by dissimilarity values among 7 sampling sites indicates that the macrobenthic communities were divided into 3 groups： stations 1, 2, 3 and 4； stations 6 and 7； and station 5 alone.

• Journal of Korean Medical classics
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• v.25 no.1
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• pp.89-115
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• 2012

Objective : Blood disease is common these days due to modern man's excessiveness in Yang heat (陽熱) and vulnerability of the Eum blood(陰血). This exposes them to warmheat/ dampness-heat diseases, where pathogenic heat easily penetrates the blood dimension(血分) creating stagnated blood(瘀血). Consequently, pathogenic symptoms in the collateral vessels increase, making it crucial to understand the pathogenic mechanism of the disease. Method : This paper examines the condition and region of the collateral vessel diseases(CVD) according to the blood diseases of Onbyeong, by analyzing each prescription's matching symptom. The disease in question in this paper is 'stagnated blood fixated in the collateral vessels'. Therefore diseases with stagnated blood in the Yang collaterals and Viscera collaterals or viscera themselves from the chapter of "On-Yeok-Ron(溫疫論)", and < Dry Blood-DaeWhangJaChungWhan(大黃蟅蟲丸) > chapter of "Geum-Gue-Yo-Rak(金匱要略)", were examined respectively. Result & Conclusion : The process of CVD according to the blood diseases of Onbyeong can be summarized as follows. First, bleeding in the Yang and Bowel collaterals, then stagnation in the Yang and Bowel collaterals, and finally stagnation in the viscera collaterals or Liver itself. The refractory nature of blood stagnation symptoms of the collateral vessels is mainly due to the characteristics of the collateral vessel itself. In structure, they are very narrow and small, situated at the most terminal part of the body where it is difficult for the Jeong Gi(精氣) to reach. Also, as they are symptomatic of degeneration of Jeong Gi, the root of the disease is very deep. Therefore to resolve blood stagnation in the cases of Ju-Gaek-Gyo and Dry Blood, general approaches using 'Gi communication(行氣)' or 'Blood vitalizing(活血)' medicinals will not suffice. Special medicinals such as crustacean and insects need to be appropriately applied.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.6
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• pp.599-607
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• 1986

Acute toxicity of crude oil (WSF) on the mortality and respiration rates of Neomysis awatschensis was examined. This experiment was conducted by static and short-term bioassay procedure. In lethal test, the test animals were exposured to 8 different concentrations to determine $LC_{50}$ value (median lethal concentration). The concentrations of total hydrocarbon of 96-hr $LC_{50}$ value at $14^{\circ}C\;and\;20^{\circ}C$ were 1.01 ppm and 0.78 ppm, respectively. $LT_{50}$ (the median lethal time) also was determined. The $LT_{50}$ of 0.56 ppm was found within 100 hours, while the $LT_{50}$ of 5.6 ppm was 21 hours at $14^{\circ}C$. At $20^{\circ}C$, the $LT_{50}$ values of 0.56 ppm and 5.60 ppm were 95 hours and 17 hours, respectively. There was little difference between two temperature experiments. The effect of WSF on respiration rate was more sensitive than that on mortality, but no considerable difference was shown between different concentrations in this experiment. The results of these experiments indicated that relatively low concentration of dissolved crude oil fraction can impact on small crustacean in the marine ecosystem.