• Natural Product Sciences
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• 제5권3호
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• pp.148-150
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• 1999

Seed lipids and proteins of Crotalaria juncea L were analyzed for fatty acids and amino acids respectively. Gas chromatographic analysis of the oil gave palmitic acid (16.01%), stearic acid (7.29%), oleic acid (14.41%), linoleic acid (54.44%) and linolenic acid (7.86%). The defatted seed cake contained all the essential amino acids except methionine and six non-essential amino acids.

• 한국유기농업학회지
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• 제19권2호
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• pp.215-228
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• 2011

본 연구는 자원 순환농법으로 환경 친화적인 고추 유기재배 기술을 확립하고자 하계 녹비작물의 재배가 시설 토양의 화학성과 고추의 생육 및 수량에 미치는 영향을 구명하고자 시험을 수행하였다. 화본과 녹비작물인 하우스솔고와 수수는 건물수량이 많았으며, 뿌리도 깊게 분포하였다. 뿌리혹선충의 밀도는 수수와 네마장황 재배 토양에 하우스솔고와 콩 재배 토양에 비하여 현저하게 감소되었다. 녹비작물의 무기성분 함량은 네마장황과 콩에서 T-N와 CaO 함량이 높았다. 녹비작물의 무기성분 중 T-N 고정량은 콩과 하우스솔고 그리고 수수가, $K_2O$와 MgO의 고정량은 하우스솔고와 수수에서 많았으며, CaO의 고정량은 콩 녹비작물에서 많았다. 녹비작물 재배 토양의 pH는 증가되었으나, 녹비작물을 환원 고추를 재배한 토양의 pH는 감소되었다. 녹비작물 재배 토양의 염류농도는 현저하게 감소되었으나, 녹비작물을 환원 후 고추를 재배한 토양의 염류농도는 증가되었다. 녹비작물 재배 토양의 유기물함량은 하우스솔고 재배 토양을 제외하면 유의적인 차이가 없었으나, 고추를 재배한 토양의 유기물함량은 감소되었다. 토양의 T-N함량은 콩을 제외하고 녹비작물 재배에 의하여 감소되었다. 녹비작물 재배 토양의 K농도는 콩 재배 토양에서 증가되었으나, 하우스솔고와 수수 재배 토양은 감소되었다. 녹비작물을 환원 고추를 재배한 토양의 K 농도는 콩과하우스솔고 재배 토양에서 증가되었다. 녹비작물 재배 토양의 Ca과 Mg 농도는 하우스솔고 재배 토양을 제외하면 유의적인 차이가 없었으나, 녹비작물을 환원 고추를 재배한 토양에 서 감소되었다. 녹비작물 재배 토양의 양이온치환용량은 네마장황과 하우스솔고 재배토양에서 감소되었으나, 수수와 콩, 무처리 토양은 증가되었으며, 녹비작물을 환원 고추를 재배한 토양은 유의적인 차이가 없었다. 녹비작물을 토양에 투입 환원 후 재배한 고추의 생육과 수량은 처리간에 유의적인 차이는 없었으나, 고추 수량은 녹비작물 재배 토양이 무처리에 비하여 7~23% 정도 증가되었다.

• Advances in Traditional Medicine
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• 제6권2호
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• pp.86-95
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• 2006

The ethanol extract of the Crotalaria juncea seeds, which showed promising antispermatogenic and antiandrogenic activities in albino mice, was taken up further for the isolation of the active fractions present in it. Two fractions that were obtained from thin layer chromatography were subjected for testing to know their antispermatogenic and antiandrogenic activities. After preliminary trials the fraction I showed maximum antifertility activity at the dose level of 200 mg/kg body weight when administered orally to the rats for 50 days. The fraction I was found to affect spermatogenesis as well as the endocrine functions of the testis as indicated by gravimetric, histopathological and biochemical changes. Further this fraction has caused degenerative changes in the seminiferous tubules and Leydig cells of the testis. The accessory reproductive organs like epididymis, seminal vesicles, vas deferens, prostrate, Cowper's gland and Levator Ani muscle showed significant malfunction. Cauda epididymal sperm count and sperm motility were reduced significantly. The treatment has also resulted in increase in the cholesterol level and alkaline phosphatase activity, and decrease in protein, glycogen, sialic acid contents and acid phosphatase activity in testis. It is noteworthy that RIA studies have shown significant reduction in serum FSH, LH and testosterone. Scanning electron microscopic observations revealed abnormalities in sperm structure.

• Advances in Traditional Medicine
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• 제4권2호
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• pp.70-76
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• 2004

Petroleum ether, benzene and alcohol extracts of the seeds of C. juncea were tested for antiimplantation and pregnancy interruption activities in female albino rats. Of these three extracts, the alcohol extract was found to be the most effective in causing antiimplantation and pregnancy interruption activities. These adverse effects on fertility are reversible upon withdrawal of the extract treatments. The alcohol extract was found to possess estrogenic activity. After subjecting to preliminary phytochemical screening, the alcohol extract showed positive tests for alkaloids, steroids, glycosides, saponins, flavonoides, fixed oils, phenols and tannins.

• Advances in Traditional Medicine
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• 제4권2호
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• pp.77-81
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• 2004

Petroleum ether, benzene and alcohol extracts of seeds of C. juncea administered orally at the dose level of 25mg/100g body weight to adult female mice for 30 days, resulted in irregular estrous cycle with prolonged estrus and metaestrus and reduced diestrus and proestrus during the experimental period. Histological studies of the ovary indicate increases in the number of atretic follicles but decreases in the number of developing follicles, Graafian follicles and corpora lutea. The total cholesterol content of the ovary is increased, whereas ascorbic acid content is decreased. The weight of the uterus and its micrometric measurement in all experimental mice are increased significantly. The alcoholic extracts showed estrogenic activity in immature mice by early opening of the vagina, premature cornification of the vaginal epithilium and increases in uterine weight. However, alcohol extract of seeds of C. juncea was more effective in causing these changes compared to other extracts. After subjecting to preliminary phytochemical screenings alcohol extract showed positive; test for alkaloids, steroids, glycosides, flavones, phenols and tannins.

• 한국유기농업학회지
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• 제19권spc호
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• pp.25-29
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• 2011

This study was performed to analyze the improvement of soil physical property and soil biota characteristics through cultivation of green manure crops for a one-year period before creation of a tea plantation as follows. The study revealed that the contents of available phosphate tended to decrease after sod-culture by green manure cultivation and open-pollination, when compared to the level before cultivation. The ratio soil porosity increased by approximately 30% when Crotalaria juncea and Sorghum bicolar L. Moench were cultivated, while the soil bacteria and fungi also increased. In a research on microfauna using a pit fall trap, the population number of the microfauna was 174 of 27 species in the plot of open-pollinated sod-culture and no organic matter application, and 268 of 26 species in the plot of Sorghum bicolar L. Moench. Consequently, the culturing tool of Crotalaria juncea recorded the highest level of species diversity at 2.5, the evenness index at 3.7 and richness at 4.6, with the lowest level of a dominance index. The ecological quotient of microfauna was 0.76 in the plot of Sorghum bicolar L. Moench, and 0.63 in the plot of Crotalaria juncea.

• 한국토양비료학회지
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• 제46권6호
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• pp.445-451
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• 2013

We researched nitrogen and green manure yield of crotalaria by seeding rate; 50, 60, 70, 80, 90 kg $ha^{-1}$ in upland soil to find out crotalaria's optimal seeding rate. Crotalaria's plant height and number of leaves increased when the harvest time was later regardless of its seeding rate. Its nitrogen content of above-ground part was 19.8 g $ha^{-1}$, and C/N ratio was 22.5. The highest nitrogen content (50.3 g $ha^{-1}$) was found in flowers part, followed by its leaves, roots and stems. The green manure yields of crotalaria increased when the harvest time was later. The green manure yield of crotalaria was biggest in 50kg $ha^{-1}$ which was low in seeding rate. It tended to decrease when the seeding rate was higher, and the nitrogen yield had the same tendency. Therefore, the appropriate seeding rate was 50kg $ha^{-1}$ and the time for application to soil was considered to be the flowering stage.

• 한국유기농업학회지
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• 제20권1호
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• pp.37-48
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• 2012

본 연구는 유기재배 배 과수원에서 녹비작물 재배에 의한 식물양분의 환원효과를 알아보기 위해서 수행되었다. 처리는 녹비작물 재배방법에 따라서, 1) 방임초생, 2) 겨울철 녹비작물 재배(호밀+헤어리베치), 3) 겨울철 녹비작물+여름철 녹비작물[그린솔고(수단그라스 품종($Sorghum$ $bicolor$ L.))+네마장황(클로타라리아 품종($Crotalaria$ $juncea$ L.))]으로 나누었다. 겨울철 녹비작물인 호밀과 헤어리베치는 각각 6.4kg/10a와 3.0kg/10a로 2008년 10월 17일에 파종하였으며, 여름철 녹비작물인 그린솔고와 네마장황은 각각 2.0kg/10a와 3.0kg/10a로 2009년 6월 4일에 전남 보성의 배 과수원 독농가에 처리하였다. 녹비작물의 건물중은 겨울+여름녹비작물>겨울녹비작물>방임초생 순으로 나타났다. 녹비작물의 건물중의 차이는 전질소와 인산 그리고 칼륨의 환원량에도 영향을 미쳤으며, 10-12년생 '신고' 배나무가 필요로 하는 질소와 인산 그리고 칼륨의 요구도에 비교해서 다소 많은 양을 공급한 것으로 판단되었다. 이러한 과다한 무기성분 공급량은 토양의 유기물과 칼륨 그리고 마그네슘 농도를 적정농도 이상으로 증가시켰다. 하지만 배나무의 영양상태의 지표인 잎의 무기성분은 녹비작물 연용에 따른 별다른 영향을 받지 않은 것으로 사료되었다.

• 한국지하수토양환경학회지:지하수토양환경
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• 제19권5호
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• pp.35-44
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• 2014

In this study, the bio-phytoremediation and phytoremediation technologies were applied to the soils contaminated with total petroleum hydrocarbons (TPH) and heavy metals to evaluate the remediation efficacy from May 2012 to December 2013. Poplar (Populus bonatii Levl.) and Sun Hemp (Crotalaria juncea L.) were selected and planted in phytoremediation practice. These plants were also utilized in the bio-phytoremediation practice, with the addition of earthworm (Eisenia fetida) and petroleum-degrading bacteria (Pseudomonos sp. NKNU01). Furthermore, physiological characteristics, such as photosynthesis rate and maximal photochemical yield, of all testing plants were also measured in order to assess their health conditions and tolerance levels in adverse environment. After 20 months of remedial practice, the results showed that bio-phytoremediation practice had a higher rate of TPH removal efficacy at 30-60 cm depth soil than that of phytoremediation. However, inconsistent results were discovered while analyzing the soil at 100 cm depth. The study also showed that the removal efficiency of heavy metals was lower than that of TPH after remediation treatment. The results from test field tissue sample analysis revealed that more Zinc than Chromium was absorbed and accumulated by the tested plants. Plant height measurements of Poplar and Sun Hemp showed that there were insignificant differences of growth between the plants in remediation plots and those in the control plot. Physiological data of Poplar also suggested it has higher tolerance level toward the contaminated soils. These results indicated that the two testing plants were healthy and suitable for this remediation study.

• 한국균학회지
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• 제46권1호
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• pp.58-68
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• 2018

클로탈라리아는 국내에서 토양개량을 위한 질소고정, 토양침식 감소, 잡초 및 선충 억제를 위해 풋거름작물로 이용하고 있다. 2014년, 클로탈라리아를 풋거름작물로 재배하는 완주 지역의 포장에서 시들음 증상이 관찰되었다. 감염된 식물체의 잎은 아래잎부터 황화 되면서 시들기 시작하였고, 위쪽 잎도 황화 되었으며, 결국 식물체는 완전히 고사하였다. 감염된 식물체 줄기에서 어두운 색의 자낭각이 다수 관찰되었고, 이 자낭각으로부터 자낭포자를 단포자 분리하여 6개 균주를 분리하였다. 균학적 특성에 의해 분리균은 Fusarium udum(완전세대: Gibberella indica)로 동정되었다. 대형포자의 정단세포는 대부분 갈고리 모양으로 굽어 있고, 소형포자는 단경자에서 false head 상으로 형성되었다. 후벽포자는 균사에서 단일 혹은 무리지어 풍부하게 형성되었다. 이와 같은 동정 결과는 translation elongation factor 1 alpha(TEF), calmodulin (CAL), histone 3 (HIS3) 유전자 염기서열 분석으로 재확인되었다. 그 결과, 분리 균주는 NCBI GanBank에 등록된 F. udum과 TEF 유전자는 94.4~96.2%, CAL 유전자는 99.7%, HIS3 유전자는 99.6~99.8%의 상동성을 보였다. 클로탈라리아와 두 품종의 콩을 대상으로 포자현탁액을 토양에 관주 접종하여 병원성 검정을 수행한 결과, 접종 14~21일 이내에 접종한 클로탈라리아와 태광콩에서 병징이 관찰되었다. 따라서 이 병을 F. udum에 의한 클로탈라리아 시들음병으로 명명하고자 제안하며, 국내에서 처음으로 보고한다.