• Asian-Australasian Journal of Animal Sciences
• /
• 제31권1호
• /
• pp.129-137
• /
• 2018

Objective: The objective of this research was to evaluate the physico-chemical, microbiological and sensorial qualities of restructured steaks processed from beef trimmings (grade I and II) and frozen beef (fresh beef as control and frozen beef). Methods: Beef trimmings from commercial butcher were collected, designated into 4 treatments differing in beef trimmings grade and freezing, processed into restructured steaks with 1% microbial transglutaminase and then analyzed for product quality. Results: The results showed that all meat from different groups could be tightly bound together via cross-linking of myosin heavy chain and actin as observed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Microbial counts of psychrotrophic and mesophilic bacteria were not affected by treatments (p>0.05), and no detectable of thermophilic bacteria were found. Regarding effect of beef trimmings grade, steaks made from beef trimmings grade II (16.03% fat) showed some superior sensorial qualities including higher tenderness score (p<0.05) and tendency for higher scores of juiciness and overall acceptability (p<0.07) than those made from beef trimmings grade I (2.15% fat). Moreover, a hardness value from texture profile analysis was lower in steaks processed from beef trimmings grade II than those made from grade I (p<0.05). Although some inferior qualities in terms of cooking loss and discoloration after cooking were higher in steaks made from beef trimmings grade II than those made from beef trimmings grade I (p<0.05), these differences did not affect the sensory evaluation. Frozen beef improved the soft texture and resulted in effective meat binding as considered by higher cohesiveness and springiness of the raw restructured product as compared to fresh beef (p<0.05). Conclusion: The results indicated the most suitable raw beef for producing restructured steaks without detrimental effect on product quality was beef trimmings grade II containing up to 17% fat which positively affected the sensory quality and that frozen beef trimmings increased tenderness and meat binding of restructured beef steaks.

• IMMUNE NETWORK
• /
• 제8권3호
• /
• pp.82-89
• /
• 2008

Background: Although a skin test is the primary option for detecting allergen-specific IgE in clinics, the serum IgE immunoassay is also important because it allows for the diagnosis of allergy without any accompanying adverse effect on the patient. However, the low detection limit of IgE levels by immunoassay may restrict the use of the method in some occasions, and improving its sensitivity would thus have a significant implication in allergy-immunology clinics. Methods: In this study, we attempted to detect specific serum IgE by using immuno-polymerase chain reaction (IPCR) which combines the antigen-antibody specificity of enzyme-linked immunosorbent assays (ELISAs) with the amplification power of PCR. Results: Our results demonstrated that Blo t5-specific serum IgE can be detected by IPCR with a 100-fold higher sensitivity than ELISA, and cross-reactivity of serum IgE to other mite allergens is able to be analyzed by using only $0.3{\mu}l$ of serum sample. Use of real-time IPCR seemed to permit more convenient determination of specific serum IgE as well. Conclusion: We believe that IPCR can serve as a valuable tool in determining specific serum IgE, especially when the amount of serum sample is limited.

• Preventive Nutrition and Food Science
• /
• 제10권3호
• /
• pp.279-284
• /
• 2005

Uncoupling protein-1 (UCP-1) plays a major role in thermogenesis, and has been implicated in the pathogenesis of obesity and metabolic disorders. The aim of this study was to estimate the effects of A-3826G polymorphism of UCP-1 gene on body fat distribution. Two hundred forty eight Korean female overweight subjects with BMI more than 25 kgfm2 participated in this study. The areas of abdominal subcutaneous and visceral fat of all subjects were measured from computed tomography cross sectional pictures of the umbilical region. Subcutaneous fat areas of upper and lower thigh were also measured. Body composition was measured by bio-impedance analysis, and serum concentrations of biochemical parameters, such as glucose, triglyceride, cholesterol etc, were also measured. Genotype of UCP-1 was analyzed by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method. The frequencies of UCP-1 genotypes were AA type; $27.8\%,\;AG\;type;\;51.2\%\;and\;GG\;type;\;21.0\%,$ and the frequency of G allele was 0.47. Body weight, BMI, WHR, SBP, DBP and body compositions were not significantly different by UCP-1 genotype. Abdominal visceral fat area was significantly higher in AG and GG type compared with AA type (p=0.009), but subcutaneous fat areas were not significantly different by UCP-1 genotype. Among biochemical parameters, LDL cholesterol level was significantly higher in GG type compared with AA and AG types (p=0.033). Among all subjects, 121 subjects finished 1 month weight loss program containing hypocaloric diet and exercise. The reduction of body weight and BMI were lower in GG type compared with AA/AG type even though statistical significances were not found (p > 0.05). These results suggest that UCP-1 genotype has a significant effect on visceral fat accumulation among Korean female overweight subjects with BMI more than $25\;kg/m^2$.

• The Plant Pathology Journal
• /
• 제36권2호
• /
• pp.170-178
• /
• 2020

The Lily mottle virus (LMoV) impedes the growth and quality of lily crops in Lanzhou, China. Recently Arabis mosaic virus (ArMV) has been detected in LMoV-infected plants in this region, causing plant stunting as well as severe foliar symptoms, and likely posing a threat to lily production. Consequently, there is a need to develop simple, sensitive, and reliable detection methods for these two viruses to prevent them from spreading. Reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assays have been developed to detect LMoV and ArMV using two primer pairs that match six conserved sequences of LMoV and ArMV coat proteins, respectively. RT-LAMP assay results were visually assessed in reaction tubes using green fluorescence and gel electrophoresis. Our assays successfully detected both LMoV and ArMV in lily plants without the occurrence of viral cross-reactivity from other lily viruses. Optimal conditions for LAMP reactions were 65℃ and 60℃ for 60 min for LMoV and ArMV, respectively. Detection sensitivity for both RT-LAMP assays was a hundredfold greater than that of our comparative RT-polymerase chain reaction assays. We have also found this relatively rapid, target specific and sensitive method can also be used for samples collected in the field and may be especially useful in regions with limited or no laboratory facilities.

• Korea-Australia Rheology Journal
• /
• 제13권1호
• /
• pp.29-35
• /
• 2001

The development of filament stretching extensional rheometers over the past decade has enabled the systematic measurement of the transient extensional stress growth in dilute and semi-dilute polymer solutions. The strain-hardening in the extensional viscosity of dilute solutions overwhelms the perturbative effects of capillarity, inertia & gravity and the kinematics of the extensional deformation become increasingly homogeneous at large strains. This permits the development of a robust open-loop control algorithm for rapidly realizing a deformation with constant stretch history that is desired for extensional rheometry. For entangled fluids such as concentrated solutions and melts the situation is less well defined since the material functions are governed by the molecular weight between entanglements, and the fluids therefore show much less pronounced strain-hardening in transient elongation. We use experiments with semi-dilute/entangled and concentrated/entangled monodisperse polystyrene solutions coupled with time-dependent numerical computations using nonlinear viscoelastic constitutive equations such as the Giesekus model in order to show that an open-loop control strategy is still viable for such fluids. Multiple iterations using a successive substitution may be necessary, however, in order to obtain the true transient extensional viscosity material function. At large strains and high extension rates the extension of fluid filaments in both dilute and concentrated polymer solutions is limited by the onset of purely elastic instabilities which result in necking or peeling of the elongating column. The mode of instability is demonstrated to be a sensitive function of the magnitude of the strain-hardening in the fluid sample. In entangled solutions of linear polymers the observed transition from necking instability to peeling instability observed at high strain rates (of order of the reciprocal of the Rouse time for the fluid) is directly connected to the cross-over from a reptative mechanism of tube orientation to one of chain extension.

• Pediatric Infection and Vaccine
• /
• 제3권2호
• /
• pp.163-167
• /
• 1996

Aseptic meningitis, the most common infection of the central nervous system, is an acute illness mostly caused by enteroviruses. Cerebrospinal fluid(CSF) has been used for the detection of enteroviral RNA but the detection has been mostly performed in a single CSF specimen obtained during the illness. A major objective was to evaluate the relation of sampling time to the recovery of enteroviral RNA in CSF. Thirty seven CSF specimens were obtained from 24 patients between May and August 1993, when an outbreak of asceptic meningitis by echovirus type 9 occurred. Enteroviral RNA in CSF was detected by polymerase chain reaction(PCR). Data about onset of symptom development were obtained by review of medical records. Enteroviral RNA was detected by PCR in 29 of 37 CSF specimens. PCR yielded positive results in 4 of 5 CSF specimens obtained on day 1 to 3, 10 of 11 on day 4 to 6, 8 of 10 on day 7 to 9, 6 of 8 on day 10 to 12, 1 of 3 on day 13 to 15 postonset. Of 11 patients from each of whom more than one CSF were obtained on different day postonset, PCR yielded positive resutls in 2 of 3 cases in whom enteroviral RNA detection was negative in the first CSF. These results indicate that two or more CSF specimens obtained within 12 days postonset are required for improving the accuracy of the diagnosis of enteroviral meningitis.

• The Plant Pathology Journal
• /
• 제36권1호
• /
• pp.76-86
• /
• 2020

Cucumber mosaic virus (CMV) is damaging to the growth and quality of lettuce crops in Lanzhou, China. Recently, however, for the first time an isolate of lettuce necrotic yellows virus (LNYV) has been detected in lettuce crops in China, and there is concern that this virus may also pose a threat to lettuce production in China. Consequently, there is a need to develop a rapid and efficient detection method to accurately identify LNYV and CMV infections and help limit their spread. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays were developed to detect the nucleoprotein (N) and coat protein (CP) genes of LNYV and CMV, respectively. RT-LAMP amplification products were visually assessed in reaction tubes separately using green fluorescence and gel electrophoresis. The assays successfully detected both viruses in infected plants without cross reactivity recorded from either CMV or LNYV or four other related plant viruses. Optimum LAMP reactions were conducted in betaine-free media with 6 mM Mg²⁺ at 65℃ for LNYV and 60℃ for 60 min for CMV, respectively. The detection limit was 3.5 pg/ml and 20 fg/ml using RT-LAMP for LNYV and CMV plasmids, respectively. Detection sensitivity for both RT-LAMP assays was greater by a factor of 100 compared to the conventional reverse transcription polymerase chain reaction assays. This rapid, specific, and sensitive technique should be more widely applied due to its low cost and minimal equipment requirements.

• 한국항만경제학회지
• /
• 제31권3호
• /
• pp.107-120
• /
• 2015

기업들의 국제물류체계는 물류비용과 시간의 절감뿐만 아니라 물류의 부가가치 창출 기능을 극대화하기 위해 급속히 발전할 전망이다. 글로벌 공급체인 운영방식인 Ro-Ro 운송은 운송시간의 정확성, 비용절감, 신속성, 안전성 향상 등에서 경쟁력이 높아 이를 활용하기 위한 전략이 급증할 것으로 전망된다. 이에 본 연구에서는 한일간 교류 물동량 분석을 통해 Ro-Ro 부두 조성의 적정 수요와 규모를 추정해 보고자 하였다. 이를 위해 한일간 Ro-Ro선의 운항속도에 근거한 운항거리를 바탕으로 1권역과 2권역으로 구분하여 부산항과의 물동량(컨테이너와 일반화물)을 분석하였다. 분석된 물동량을 바탕으로 Ro-Ro선 이용 가능성이 높은 품목의 비중을 추정한 후 한일간 Ro-Ro 부두의 이용가능 물동량을 추정하였다. 추정된 물동량을 처리하기 위해서는 2만톤급 2개 선석과 장치장 10만$m^2$ 정도가 필요한 것으로 판단된다. 부산항에 Ro-Ro 전용 부두 조성을 통해 컨테이너 일변도의 항만정책을 탈피하고, 글로벌 기업들의 수요에도 대응할 수 있을 것이다.

• 한국언론정보학보
• /
• 제56권
• /
• pp.26-47
• /
• 2011

언론의 상업주의란 목적과 수단이 전치된 상황을 의미한다. 다시 말해 언론의 목적을 제대로 수행하기 위한 수단으로서 적정한 이윤이 필요하지만, 이윤에 집착함으로써 그 목적을 잃어버리거나 저널리즘의 질을 떨어트리는 행태이다. 상업주의화는 상업주의의 과정이 심화되는 경향을 말한다. 서구에서는 1990년대 중반 미디어 시장에 대한 규제완화와 더불어 언론의 상업주의가 득세하고 있다. 한국도 이명박 정부가 들어서면서 언론과 관련한 대부분의 법률들이 서구와 유사한 규제완화의 길로 접어들었다. 이 때문에 한국에서도 언론의 상업주의화에 대한 우려가 팽배하고 있다. 특히, 신문과 방송의 교차소유 허용과 함께 4개의 종합편성채널이 시장에 진입함으로써 거대한 지각변동이 예상된다. 제한된 광고시장에서 무한 출혈경쟁이 불가피할 전망이다. 이들 종합편성채널은 미디어렙을 통하지 않고 직접 광고판매가 가능함으로써 신문과 연계된 다양한 광고도 선보일 것이다. 이 때문에 언론의 상업주의화에 대한 우려가 기우에 그치지 않는 것이다. 이러한 언론 상업주의의 문제점을 보다 깊이 고찰하기 위해 본 논문은 예술계와 선도국의 사례를 비교사적으로 접근했다. 이를 통해 언론의 본질을 재조명하는 한편, 취재보도의 윤리와 언론인의 사명이 중요함을 밝혔다.

• 한국항만경제학회지
• /
• 제35권4호
• /
• pp.17-30
• /
• 2019

본 연구는 베트남 북부지역 하노이, 하이퐁 지역을 중심으로 최근 이루어지고 있는 국내물류기업들의 비즈니스 모델을 검토해보고 시사점을 제시하였다. 기 진출한 물류업체(선사, 물류업체, 현지업체 등)와의 협력을 통한 물류서비스를 제공하는 비즈니스 모델들을(연안운송, 물류센터 및 ICD 운송, 콜드체인, 국경통과, 항만개발 등) 소개하였다. 이 모델별 단기/중기로 나누어 파트너 쉽 방안을 제시하면 단기적 사업으로는 Warehouse, ICD, Trucking 사업 등을 들 수 있으며 기 진출한 우리나라업체들과의 협업이 가능하며 창고부문 투자 이외에 ICD와 트럭킹 사업은 로컬업체와 조인트 벤처 투자(로컬업체 51% 이상 투자)가 필요하다. 또한, 장기적으로는 연안운송, 국경간 운송, 트럭킹, Deep Sea 신항만 투자 등을 고려할 수 있으며 전부 현지파트너 쉽이 필요한 사업이어서 로컬 업체는 51% 이상의 투자지분이 요구된다.