• Horticultural Science & Technology
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• v.30 no.5
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• pp.613-616
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• 2012

The new Cymbidium variety 'Pink Glory' (Palace Court 'Jacky' ${\times}$ Lucky Rainbow 'Saint Rapine') was developed at the National Institute of Horticultural & Herbal Science, Rural Development Administration in 2006. A cross was made in 1995 between C. Palace Court 'Jacky' with a medium sized bright pink colored flowers, and C. Lucky Rainbow 'Saint Rapine' with pink colored flowers. In 1999, one line was selected based on the flower color, leaf shape, flower stalk, and vigorous growth. Evaluation and selection trials were conducted from 2000 to 2004. 'Pink Glory' has a light pink sepal (RHS, RPN66D), red lip (R52B) and a leaf length of 87.5 cm. The average number of flowers on each peduncle was 20.5 and the flower diameter was 8.6 cm. The general impression of petals and sepals showed some incurved and some spreading. 'Pink Glory' has a vigorous growth character and a rounded flower shape. It has an adequate peduncle height (66.5 cm) compared to leaf length (87.5 cm) and starts to bloom in late January under optimal cultural conditions.

• Journal of Plant Biotechnology
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• v.39 no.4
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• pp.219-224
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• 2012

Alstroemeria plants are widely cultivated in many countries especially in Western Europe and North America and popularity has increased in recently due to its long-base life, large variety of colors and low energy requirement during cultivation period. So far, more than 60 species have been released on the commercial market in the world. To meet the demand of consumer and develop the elite Alstroemeria cultivars, conventional breeding including cross-hybridization and selection as well as mutation breeding were used. However, as other important ornamental plants such as lily, rose, carnation and orchids accepted the biotechnological methods, this newly-born approach should be applied and developed an optimized the genetic transformation system. Then, this biotechnological approach can be fused with the conventional breeding methods and thus can be contributed to the production of elite Alstroemeria plants containing agriculturally good genetic traits which are useful for the both farmers and consumers in the future. In this paper, we reviewed the botanical and genetical features of Alstroemeria plants and its biotechnological approaches in the last decades.

• Journal of Fashion Business
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• v.6 no.2
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• pp.110-123
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• 2002

This study investigated the influence of cultural and general trend of the times on fashion trends and the hybridization of these trends. The objectives of this study were to suggest new approach method of the fashion forecasting and to find out the cultural meanings and values of fashion. Key words that explains fashion trend from spring/summer of 1995 to fall/winter of 2002 were collected from professional fashion trend journals. These terms were analysed with domain analysis based on the semantic relationship. The frequency of their appearance in each season and the aspect of their appearance in each season were analysed. The hybrids of the fashion trends were analysed longitudinally and cross-sectionally as well by investigating the cover terms that were found as a result of domain analysis. The method used in this study can be applied to future study of fashion trend in that it offers an objective view of fashion trend and can allow researchers to study fashion trend in a concrete way.

• Applied Biological Chemistry
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• v.32 no.1
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• pp.64-72
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• 1989

Near full length cDNA clones encoding the rice seed storage protein, prolamine, were isolated and divided into two homology classes based on cross-hybridization and DNA sequencing analysis. These cDNA clones contain a single open reading frame encoding a putative rice prolamine precursor(M.W.=17,200) possessing atypical 14 amino acid signal peptide. Clones of these two homology classes diverge mainly by insertions/deletions of short nucleotide stretches and point mutations. The deduced primary structures of both types of prolamine polypeptides are devoid of any major tandem repetitive sequences, a feature prevalent in other cereal prolamines. No significant homology teas detected between the rice prolamine and other cereal prolamines, indicating that the rice gene evolved from a different ancestor that gave rise to other cereal prolamine genes. Developing wheat and rice endosperms were examined using ultrathin sections prepared from tissues harvested at various days after flowering. By immunocytochemical localization techniques, wheat prolamines are localized within vesicles from Golgi apparatus and in homogeneous regions of protein bodies. The involvement of the goli apparatus in the packaging of wheat prolamines into protein bodies indicates a pathway which differs from the mode of other cereal prolamines and resembles the mechanism employed for the storage of rice glutelin and legume globulins.

• Horticultural Science & Technology
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• v.32 no.3
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• pp.415-419
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• 2014

A Cymbidium variety 'Snow Bell' (C. kanran ${\times}$ C. Jack Frost) was developed at the National Institute of Horticultural & Herbal Science, Rural Development Administration in 2011. A cross between C. kanran with small-sized greenish colored flowers and C. Jack Frost with bright pink colored flowers, was conducted in 1999. Based on flower color, leaf shape, flower stalk, and vigor one line was selected. Evaluation and selection trials were conducted from 2006 to 2010. 'Snow Bell' has a light pink sepal (RHS, R56D), red lip (R56A) and a leaf length of 47.5 cm. The average number of flowers on each peduncle is 13.8 and the flower diameter is 6.2 cm. The characteristics of petals and sepals show some incurve and some spreading. 'Snow Bell' has a vigorous growth and a round flower. It has an adequate peduncle height of 50.7 cm compared with leaf length of 47.5 cm, and start blooming in December. In electronic nose analysis, the degree of fragrance between 'Snow Bell' and the C. kanran was similar and both of them showed strong scents, although they had different fragrance patterns.

• Journal of Crop Science and Biotechnology
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• v.10 no.2
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• pp.86-91
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• 2007

A representative group of 38 improved basmati lines including maintainers of sterile lines were studied for genetic diversity utilizing Mahalanobis $D^2$ statistics. A wide diversity was observed having ten clusters with high intra- and inter-cluster distance. Heterosis was estimated utilizing the cytoplasmic male sterile lines from the clusters having high intra- and inter-cluster distance. Highly heterotic hybrids were obtained from the hybridization programme. Cross combinations IR68281A/Pusa 1235-95-73-1-1, IR68281A/RP 3644-41-9-5, Pusa 3A/UPR 2268-4-1, IR 68281A/Pusa Basmati-1, IR68281A/BTCE 10-98, and IR58025A/HKR 97-401 were found to be highly heterotic for grain yield/plant with other agronomic and quality traits. Additionally, a positive association of intra-cluster distance with heterosis was observed, which could be utilized as a guideline for predicting heterosis in basmati hybrid rice breeding program. Also, a positive association between inter-cluster distance and heterosis was observed.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.357-369
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• 2010

Cymbidium is horticulturally important and has been one of the most commercially successful orchid plants as well as cut flowers around the world including Korea. Up to now, a huge number of elite Cymbidium cultivars have been released on the commercial market via cross-hybridization, mutation and polyploidization breeding techniques. To investigate on breeding system in Cymbidium, we inquired the brief history and techniques of breeding and the current status on Cymbidium breeding in Korea. Also, the general propagation process of elite Cymbidium lines via tissue culture should be presented. However, the slow process of conventional breeding and the lack of useful genes in Cymbidium species delays the introduction of new cultivars to the commercial market. To solve these limitations, efficient regeneration and genetic transformation systems should be established in the improvement of Cymbidium breeding program. During the last several decades, some progress has been made in tissue culture and genetic transformation in Cymbidium species. We review the recent status of tissue culture and genetic transformation systems in Cymbidium plants.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.379-387
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• 2010

Oilseed rape (Brassica napus L.) is an important crop due to its high oil content in the seed. Recently, the demand for the improvement of crop for biodisel energy source is increased as oil prices in the world has increased dramatically. Until now, oilseed rape breeding was carried out by cross-hybridization between different varieties and related germplasms. However, like as many other crops, the application of tissue culture and gene transformation systems has been introduced into oilseed rape breeding program including the development of transgenic canola plants. In this study, we reviewed a history of tissue culture and genetic transformation research in oilseed rape plants and indicated some important aspects for the production of transgenic oilseed rape plants.

• Journal of Sensor Science and Technology
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• v.33 no.3
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• pp.139-146
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• 2024

Surface-enhanced Raman scattering (SERS) enables the detection of various types of π-conjugated biological and chemical molecules owing to its exceptional sensitivity in obtaining unique spectra, offering nondestructive classification capabilities for target analytes. Herein, we demonstrate an innovative strategy that provides significant machine learning (ML)-enabled predictive SERS platforms through surface-engineered graphene via complementary hybridization with Au nanoparticles (NPs). The hybridized Au NPs/graphene SERS platforms showed exceptional sensitivity (10^-7 M) due to the collaborative strong correlation between the localized electromagnetic effect and the enhanced chemical bonding reactivity. The chemical and physical properties of the demonstrated SERS platform were systematically investigated using microscopy and spectroscopic analysis. Furthermore, an innovative strategy employing ML is proposed to predict various analytes based on a featured Raman spectral database. Using a customized data-preprocessing algorithm, the feature data for ML were extracted from the Raman peak characteristic information, such as intensity, position, and width, from the SERS spectrum data. Additionally, sophisticated evaluations of various types of ML classification models were conducted using k-fold cross-validation (k = 5), showing 99% prediction accuracy.

• Annals of Clinical Microbiology
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• v.18 no.2
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• pp.37-43
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• 2015

Background: Tuberculosis is globally the most important cause of death from single pathogen. Rapid and accurate identification of mycobacteria is essential for the control of tuberculosis. We evaluated a fluorescence in situ hybridization (FISH) method using peptide nucleic acid (PNA) probes for the differentiation of Mycobacterium tuberculosis complex (MTB) and nontuberculous mycobacteria (NTM) in direct smears of sputum specimens. Methods: The cross-reactivity of MTB- and NTM-specific PNA probes was examined with reference strains of M. tuberculosis ATCC 13950, Mycobacterium kansasii ATCC 12479, Mycobacterium fortuitum ATCC 6841, several clinical isolates of mycobacteria (Mycobacterium abscessus, Mycobacterium avium, Mycobacterium intracellulare, Mycobacterium gordonae and Mycobacterium chelonae), and 11 frequently isolated respiratory bacterial species other than mycobacteria. A series of 128 sputa (89 MTB culture positive, 29 NTM culture positive, and 10 under treatment culture negative) with grades of trace to 4+ were used to evaluate the performance of the method. Results: The MTB- and NTM-specific PNA probes showed specific reactions with the reference strains of MTB and M. kansasii and clinical isolates of mycobacteria except M. fortuitum ATCC 6841, and no cross-reactivity with other tested bacteria. The PNA probe-based FISH assay for detection of MTB had a sensitivity and specificity of 100%, respectively. The sensitivity and specificity of the NTM-specific PNA probe was 100%. The smear grades of the PNA FISH test were same as with those of the fluorescence AFB stain in 2+ or higher grade. Conclusion: Detection and differentiation based on PNA FISH is sensitive and accurate for detecting mycobacteria and for differentiating MTB from NTM in clinical sputum smears.