• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.196-196
• /
• 2017

Oilseed crop Camelina (Camelina sativa L.) is a suitable for biodiesel production that has high adaptability under low-nutrient condition like marginal land and requires low-input cost for cultivation. Enhanced abiotic stress tolerance of Camelina is very important for oil production under the wide range of different climate. CsRCI2s (Rare Cold Inducible 2) are related proteins in various abiotic stresses that predicted to localized at plasma membrane (PM) and endoplasmic reticulum (ER). These proteins are consist of eight-family that can be divided into tail (CsRCI2D/E/F/G) and no-tail (CsRCI2A/B/E/H) type of C-terminal. However, it is still less understood the function of C-terminal tail. In this study, CsRCI2D/H genes were cloned through gateway cloning system that used pCB302-3 as destination vector. And we used agrobacterium-mediated transformation system for generation of overexpression (OX) transformants. Overexpression of target gene was confirmed using RT-PCR and segregation ratio on selection media. We analyzed physiological response in media and soil under abiotic stresses using CsRCI2D and CsRCI2H overexpression plant. To compare abiotic stresses tolerance, wild type and CsRCI2D/H OX line seeds were sown on agar plate treated with various NaCl and mannitol concentration for 7 days. In the test of growth rate under abiotic stress on media, CsRCI2H OX line showed similar to NaCl and mannitol stress. In the other hand, CsRCI2D OX line showed to be improved stress tolerance that especially increased in 200mM NaCl but was similar on mannitol media. In greenhouse, WT and CsRCI2D/H OX lines for physiological analysis and productivity under abiotic stresses were treated 100, 150, 200mM NaCl. Then it was measured various parameters such as leaf width and length, plant height, total seed weight, flower number, seed number. CsRCI2H OX line in greenhouse did not show any changes in physiological parameters but CsRCI2D OX line was improved both physiological response and productivity under NaCl stress. Among physiological parameters of CsRCI2D OX line under NaCl stress, leaf length and width were observed shorter than WT but it were slightly longer than WT in 200mM NaCl stress. Furthermore, total seed weight of CsRCI2D OX line under stress displayed to decrease than WT in normal condition, but it was gradually raised with increasing NaCl stress then more than WT relatively. These results suggested CsRCI2D might be contribute to improve abiotic stress tolerance. However, function of CsRCI2H is need to more detail study. In conclusion, overexpression of CsRCI2s family can generate various environmental stress tolerance plant and may improve crop productivity for bio-energy production.

• Journal of Forest and Environmental Science
• /
• v.34 no.4
• /
• pp.338-351
• /
• 2018

This study was conducted in a long-term experimental forest in the central hardwoods region of southwestern Michigan to retrospectively examine the role of past forest management practices and climate on red oak (Quercus rubra L.) productivity. Initially, in 1971, plots within the experimental forest were treated separately with a clearcut and shelterwood regeneration harvest in an attempt to increase red oak regeneration. From 1987-1989, a new study was initiated within a portion of the clearcut and shelterwood plots to evaluate the effectiveness of additional oak crop tree release using mechanical and chemical applications. Cumulative diameter and mortality rates of 719 red oaks were monitored across the four silvicultural treatments: Clearcut-A (clearcut without additional release treatment), Clearcut-B (clearcut with additional release treatment), Shelterwood-A (shelterwood without additional release), and Shelterwood-B (shelterwood with additional release) plus an untreated control. Increment cores were obtained from red oak trees and neighboring competitor species. Tree-ring analyses (dendrochronology) were applied to examine the effect of these silvicultural treatments and climatic factors (temperature and precipitation) on red oak productivity. The results indicated that crop tree release following a clearcut or shelterwood harvest reduced mortality rates and thus increased survival of red oak. Red oak in control plots or plots only receiving the initial regeneration harvesting treatment and no additional competition release were negatively affected by climatic stress, which included summer moisture stress. In contrast, red oak in plots that received the competition release treatment from shade tolerant tree species not only had higher tree level productivity (i.e., tree basal area) and lower mortality rates, but were also relatively more resilient to climatic stress by showing limited or no associations between climate and growth.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.46 no.1
• /
• pp.1-5
• /
• 2001

Field experiment was conducted during 1993-94 season to determine the pattern of nutrient uptake and productivity of maize/sweet potato intercropping system. Four levels of nitrogen (0, 50, 100 and 150kg N ${ha}_{-1}$) and four levels of potassium (0, 40, 80 and 120kg $K_2$O ${ha}_{-1}$) formed treatment variables. Plants were sampled periodically to determine dry matter and tissue concentrations of N and K in the individual plant components of intercropped maize and sweet potato. Nitrogen and potassium fertilizer did not interact significantly to nutrient uptake by any plant parts of intercropped maize and sweet potato. But application of N fertilizer independently enhanced N uptake in all the plant parts of maize and sweet potato. The uptake of N in leaf, leaf sheath, stem, husk, and cob of maize increased upto 90 days after planting (DAP) but grain continued to accumulate N till its maturity. Sweet potato exhibited a wide variation in N uptake pattern. Sweet potato leaf shared the maximum uptake of N at 50 DAP which rapidly increased at 70 DAP and then declined. Declination of N uptake by petiole and stem were observed after 120 DAP whereas N uptake by tuber increased slowly upto 90 DAP and then rapidly till harvest. Rate of applied K had very little effect on the uptake patterns in different components of intercropped maize. Pattern of K uptake by leaf, petiole and stem of sweet potato showed almost similar trend to N uptake. But uptake of K by tuber increased almost linearly with the K application. Pattern of N and K uptake by grain and tuber paralleled the grain yield of maize and sweet potato respectively. Intercropped productivity of maize and sweet potato found to be better by the application of 100kg N and 120 kg $K_2$O ${ha}_{-1}$

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.26 no.4
• /
• pp.311-317
• /
• 1981

As the low labor-productivity is being floated up to be the most limiting factor in barley cropping, the endeavor of many researchers concerned have been concentrated on recovering from that. For this reason, in this experiment 8 kinds of sowing methods were employed to survey the yield productivity and the labor-requirement of each methods. And with promising the possibility of consistent mechanization, both prediction of the assurable requirement of labor investment and calculation of the labor productivity in each method were estimated so that the possibility of labor-saving in barley cultivation investigated. Also sowing methods for labor-saving cultivation were presented according to the labor-structure of farm as divided with the phases.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.162-162
• /
• 2017

Atractylodes japonica and Atractylodes macrocephala belongs to Atractylodes genus and theirs roots are used as 'Baek-chul'(White atractylodes rhizome) in Korea Pharmacopoeia. 'Baek-chul' widely used in traditional herbal remedies in Asia. Concurrently, the 'Baek-chul' demand enormously increased in the market due to the growing interest in favoring health benefits but it is depend on import from China. Thus we conducted breeding research to solve the problems associated with domestically cultivated 'Baek-chul' such as low productivity and low disease tolence. We crossed A. japonica (female parent) and A. macrocephala (male parent) in the greenhouse in September 2000. the next season 119 crossed line seeds were collected and germinated in the green house. Among them we selected the good lines in the same season. then conducted replicated yield trials (RYT) in 2014 to local adaptability test (LAT) in 2015-2016. The results showed that the 'AJM16' line has beneficial charactersitcs like, increased plant height, large rhizome diameter, narrow leaf width compared with a 'Sang-chul' (check cultivar) and varied flower color from A. japonica (female parent). The line 'AJM16' was also exhibited decreased anthracnose symptoms(Colletotrichum gloeosporides Penz.). Simultaneously the root yield of AJM16 was 2,409 kg per are, which was increased 130% higher yield compared to 'Sang-chul' (check cultivar) at three various regions from 2015 to 2016. Therefore, we named AJM16 as new cultivar 'Man-chul'. According the results, we have developed a new Atractylodes spp. hybrid cultivar 'Macn-chul' with high yielding and disease tolerance by National Institute of Horticulture and Herbal Science, RDA, in 2016.

• Korean Journal of Soil Science and Fertilizer
• /
• v.50 no.6
• /
• pp.509-519
• /
• 2017

Crop simulation models are valuable tools for estimating crop yield, environmental factors and management practices. The objective of this study was to evaluate the effect of soil types on barley productivity using CERES (Crop Environment REsource Synthesis)-barley, cropping system model. So the behavior of the model under various soil types and climatic conditions was evaluated. The results of the sensitivity analysis in temperature, $CO_2$, and precipitation showed that soil types had a direct impact on the simulated yield of CERES-barley model. We found that barley yield in clay soils would be more sensitive to precipitation and $CO_2$ in comparison with temperature. And the model showed limited accuracy in simulating water and nitrogen stress index for soil types. In general, the barley grown on clay soils were less sensitive to water stress than those grown on sandy soils. Especially it was found that the CERES model underestimated the effect of water stress in high precipitation which led to overprediction of crop yield in clay soils. In order to solve these problems and successfully forecast grain yield, further studies on the modification of the water stress response of crops should be considered prior to use of the CERES-barley model for yield forecasting.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.65 no.4
• /
• pp.327-338
• /
• 2020

In recent years, the temperature of Korea has been rapidly increasing due to global warming. Over the past 40 years, the temperature of Korea has risen by about 1.26℃ compared to that in the early 1980s. By region, the west region of the Gangwon Province was the highest at 1.76℃ and the Jeonnam Province was the lowest at 0.96℃. As the temperature continues to rise, it is expected that the rice yield will decrease in the future using the current standard cultivation method. As a result of global warming, the periods in which rice cultivation could be possible in regions each year has increased compared those to the past, showing a wide variety from 110 days in Taebaek to 180 days in Busan and Gwangyang. In addition, the transplanting time was delayed by 3-5 days in all regions. The average annual yield of rice showed an increasing trend when we analyzed the average productivities of developed varieties for cooked rice since the 1980s, especially in the early 1990s, which showed a rapid increase in productivity. The relationship between the average temperature at the time of development and the rice yield was divided into the periods before and after 1996. The higher the average temperature, the lower the yield of the developed varieties until 1996. However, since 1996, the increase in the average temperature did not show a trend in the productivity of the developed varieties. The climate change adaptability of developed rice varieties was investigated by analyzing the results of growing crops nationwide from 1999 to 2016 and the change in the annual yields of developed varieties and recently developed varieties as basic data to investigate the growth status of the crops in the country. As a result of annual comparisons of the yields of Taebongbyeo (2000) and Ungwangbyeo (2004) developed in the early 2000s for Odaebyeo, which was developed in the 1980s, the annual yields were relatively higher in varieties in the 2000s despite the increase in temperature. The annual yields of Samgwangbyeo (2003) and Saenuribyeo (2007), which were recently developed as mid-late-type varieties, were higher than those of an earlier developed variety called Chucheongbyeo, which was developed in the 1970s. Despite the rapid increase in temperature, rice cultivation technology and variety development are well adapted to climate change. However, since the biological potential of rice could reach its limit, it is necessary to develop continuous response technology.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.160-160
• /
• 2017

Drought stress is a major constraint of crop development and productivity. Plants have evolutionally developed several mechanisms at the molecular, cellular, and physiological levels to overcome drought stress. The basic Leucine zipper (bZIP) transcription factor (TF) family members are starting to be concerned about their roles in drought stress responses. In this study, we functionally characterized OsbZIP66, a rice group-E bZIP TF, to be associated with rice drought tolerance mechanisms. Expression of OsbZIP66 was significantly induced upon treatments of rice plants with drought, high salinity, and ABA. These observations and the fact that the OsbZIP66 promoter contains ten ABA-responsive elements suggest that OsbZIP66 is up-regulated by drought stress in an ABA-dependent manner. Overexpression of both OsbZIP66 in a whole plant body and specifically in roots enhanced drought tolerance of rice plants, indicating that the rice drought tolerance positively correlates with the expression levels of OsbZIP66. Thus, our results demonstrated that OsbZIP66 has a potential for use in biotechnological development of high-yielding rice plants under drought conditions.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.121-121
• /
• 2017

The productivity of rice has been influenced by various abiotic factors including temperature which cause to limitations to rice yield and quality. Rice yield and quality are adversely affected by high temperature globally. In the present study, four Korean four cultivars such as Dongan, Ilpum, Samkwang, Chucheong were investigated in order to explore molecular mechanisms of high temperature at seedling stage. Rice seedlings grown at $28/20^{\circ}C$ (day/night) were subjected to 7-day exposure to $38/28^{\circ}C$ for high-temperature stress, followed by 2-D based proteomic analysis on biological triplicates of each treatment. The growth characteristics demonstrated that Dongan is tolerant while Ilpum is sensitive to high-temperature stress. High temperature has an adverse effect in the seedling stage both in high temperature sensitive and tolerant cultivar. Two-dimensional gels stained with silver staining, a total of 722 differential expressed protein spots (${\geq}1.5-fold$) were identified using Progenesis SameSpot software. However, a total of 38 differentially expressed protein spots were analyzed by LTQ-FT-ICR MS. Of these, 9 proteins were significantly increased while 10 decreased under high-temperature treatment. Significant changes were associated with the proteins involved in the carbohydrate metabolism, photosynthesis, and stress responses. Proteome results revealed that high-temperature stress had an inhibitory effect on carbon fixation, ATP production, and photosynthetic machinery pathway. The expression level of mRNA is significantly correlated with the results obtained in the proteome investigation. Taken together, these findings provide a better understanding of the high-temperature resistance by proteomic approaches, providing valuable insight into improving the high-temperature stress tolerance in the global warming epoch.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.50 no.1
• /
• pp.1-4
• /
• 2005

This experiment was conducted to investigate the soil nitrogen credit of biological nitrogen fixation (BNF) and the nitrogen balance of soybean in soybean-barley cropping systems. Soybean cultivar, Shinpaldalkong2 and barley cultivar, Olbori, were used in soybean mono-cropping (SM), barley monocropping (BM), and barley­soybean double cropping system. The barley-soybean double cropping system was treated with two different levels of nitrogen fertilizers, 0 nitrogen fertilizer (BS-F0), and standard nitrogen fertilizer (BS-F1). Nitrogen and organic matter concentrations in soil of BS-F1 plot on October, 2001 were increased $4.8\%\;and\;5.9\%$, respectively, compared with those on October, 2000. The ranges of BNF rate in soybean were $69.1\~ 88.2\%$ in two years, and the rate was the highest in BS-F0 plot and the lowest in SM plot. The ranges of nitrogen harvest index (NHI) in all treatments were $83.9\~86.7\%$. The yield was 270 kg/10a in BS­F1 plot and 215 kg/10a in BS-F0 plot. However, the nitrogen balances were +0.6 kg/10a of gain of soil nitrogen in BS-F0 plot and -0.4 kg/10a of loss of soil nitrogen in BS-F1 plot. In comparisons of SM and BS-F1 plots, although the seed yields were similar in two plots, the loss of soil nitrogen was higher in SM than BS-F1 plot. Overall, our results suggest that barley-soybean double cropping system was more effective in respect to seed productivity and soil nitrogen conservation than soybean monocropping system, and the N credit to following crops by soybean cultivation was identified in soybean double cropping system.