• 한국약용작물학회지
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• 제11권1호
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• pp.19-23
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• 2003

초임계추출(SFE)를 이용한 초피나무 과피 중 신미성분의 최적추출조건을 조사하였다. 최적 추출 압력은 $300kg/cm^2$ 이었으며 온도는 $60^{\circ}C$, 추출 부피는 $12\;m{\ell}$, flow rate는 3m/min 그리고 modifier로 사용한 methanol의 함량비율은 20%였다. 추출시간은 초반 20분 안에 대부분의 신미성분이 추출되었다. 또한 용매추출법과 비교한 결과 추출효능이 약 40%정도 향상되었고 시간은 20분으로 단축되었으며 유기용매 methanol은 12 ml밖에 소요되지 않을 뿐만 아니라 주요한 추출용매로 무독성 액화$CO_2$를 사용하기에 독성 유기용매의 다량 사용을 피할 수 있는 친환경적이고 경제적인 방법으로 생각되어 진다.

• 한국작물학회지
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• 제40권6호
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• pp.711-715
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• 1995

수도 저온발아성이 양호한 품종의 육성을 위한 효율적인 저온발아성 검정 조건을 모삭하기 위해 최근 육성된 신품종과 도입품종을 합하여 50품종을 공시, 1994년 영남농업시험장에서 13 , 16 , 25 에서 20일간 발아시험을 실시한 바, 그 결과를 요약하면 다음과 같다. 1. 13 , 16 , 25 에서 발아시킨 50품종들의 발아율 기초통계량 분석 결과, 13 11일 치상일에서 평균발아율이 43.8%, 발아율의 절위가 92, 그리고 분산이 748로 가장 높아 품종간 저온발아성의 차가 가장 현저한 조건이었다. 2. 13 11일 치상일의 발아율과 평균발아일수, 발아계수와의 상관에서 고도의 상관이 검증되어 13 11일 치상일의 발아율로써 저온발아성을 나타내는 지표로 시용 가능할 것으로 여겨진다. 3. 13 11일 치상일의 발아율을 근거로 공시품종들의 저온발아성을 구분해본 바, 동진벼 등 11품종이 저온발아성이 양호하였고 상주벼 등 17품종이 저온발아성이 매우 불량하였다. 4. 공시품종의 저온발아성과 몇가지 미질관련형질들과의 상관에서 출수 소요일수 및 아밀로스함량은 고도의 정의 유의상관이, 입폭과는 부의 유의상관이 인정되었다.

• Reproductive and Developmental Biology
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• 제34권3호
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• pp.247-252
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• 2010

This study was carried out between 2008 and 2009 in four dairy farms to investigate the effect of feeding of whole crop barley silage on the reproductive performance of Holstein heifers. Two diets, mixed hay or whole crop barley silage separately from concentrate were fed 6-month old Holstein heifers (=37). In control group (=CON), heifers (n=16) were fed 6 kg (/head) mixed hay and 4 kg (/head) commercial diet. In whole crop barley silage group (=WBS), heifers (n=21) were fed 10 kg (/head) whole crop barley silage, 4 kg (/head) mixed hay and 2 kg (/head) commercial diet. To manage body weight gain, the body condition score of heifers were measured every month. The results obtained were as follows: 1. Body weight in CON and WBS heifers at 13-, 14-, 15- and 17-month old were $340{\pm}17.9$ and $342{\pm}13.6\;kg$, $356{\pm}15.7$ and $366{\pm}14.7\;kg$, $382{\pm}13.1$ and $387{\pm}14.4\;kg$, and $429{\pm}15.0$ and $417{\pm}10.3\;kg$, respectively. 2. Body condition score in CON and WBS heifers at 9-, 12-, 15- and 17-month old were $2.88{\pm}0.04$ and $2.80{\pm}0.04$, $2.88{\pm}0.04$ and $2.80{\pm}0.04$, $2.89{\pm}0.08$ and $3.00{\pm}0.07$, and $2.89{\pm}0.08$ and $3.00{\pm}0.07$, respectively. 3. Average age of sexual maturity in CON and WBS heifers were $437.3{\pm}9.9$days and $939.6{\pm}12.5$days, WBS group heifers were significantly shorter (p<0.05) than CON group heifers. 4. First-service conception rates in CON or WBS group were 81.3% (13/16) and 66.7% (14121), respectively, and cumulative conception rate to 2nd artificial insemination were 87.5% for CON and 85.7% for WBS group. Conception rate was not different between treatments.

• 한국작물학회지
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• 제37권6호
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• pp.534-540
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• 1992

벼 건답직파재배에서 온도와 파종심도를 달리 하여 벼 품종의 출아와 중배축 신장을 조사한 결과를 요약하면 다음과 같다. 1. 온도가 높고 파종심도가 얕을수록 출아율이 높았으며 출아소요기간이 짧았다. 2. 각 처리온도 별로 파종심도 3cm이하에서는 출아율이 큰 차이가 없었으나 5cm이상이 되면 현저히 낮아졌다. 3. 파종 30일 후의 초장과 엽수는 온도가 낮을수록 감소하였고 파종심도가 5cm 이상이 되면 생육이 현저히 저하하였다. 4. 온도가 높고 파종심도가 5cm 이상일 때는 중배축과 하위절간의 신장이 컸다. 5. 파종심도 6cm에서 벼 품종들 중 출아율이 높았고 품종은 오수벼, 탐진벼이었고 중배축의 신장은 품종간 차이가 현저하여 5～16mm의 분포를 나타내었으며 제 1본엽의 출현이 지중 3～4cm에 이루어져서 파종심도는 지중 3～4cm가 적당한 것으로 판단되었다.

• 한국작물학회지
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• 제33권s01호
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• pp.86-97
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• 1988

Sesame(Sesamum indicum L.) is probably the most ancient oilseed crop known in the world. The seed of sesame is used in a variety of ways as food. The whole seed may be eaten raw, either roasted or parched, or fed to birds and stock. Sesame oil is used as a salad or cooking oil, in shortening, margarine and in the manufacture of soap. Minor uses are as a fixative in the perfume industry and formerly as a carrier for fatsoluble substances in pharmaceuticals such as penicillin. One of the minor constituents of sesame oil, sesamin, is used for its synergistic effect in pyrethrin insecticides, in addition of a small quantity of this substance markedly increases the effectiveness of fly sprays. The meal remaining after oil extraction can be used as and animal feed-stuff or as manure. In general sesame meal is considered to be equal to cottonseed or soybean meal as a protein supplement for livestock and poultry. It is especially high in certain amino-acids such as methionine, which is low in soybean meal, and thus can be combined with it or similar meal to form a more balanced ration. An attempt to summarize the literature review on quality improvement of sesame was made to discuss the accomplishments of the past and perspectives in the future. The reviews on quality improvement of sesame were mainly discussed in connection with the cultural practices and genetic informations in current status. The emphasis focussed on environmental variation of quality in cultural practices, such as harvest time, variety by location, climatic condition, fertilizer application, and growth regulator treatment. On the genetic variation of quality, it was discussed on variety background, mutation breeding, correlations, and inheritance of quality related characteristics. It also was discussed on relationship between quality and plant traits, storage condition or period, and seed coat color. Moreover, current research status were reviewed on some minor elements such as sesamin, oxalic acid, and trypsin inhibitor. As a results of the review, the lack of an effort to quality improvement in each utilization area was indicated as a problem area. More active efforts for the improvement of quality were also insufficient to incorporate the available genes for quality in breeding method or collection and analysis of breeding materials. Therefore, researches in the future would be recommended to emphasize on these problem areas.

• 한국작물학회지
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• 제55권1호
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• pp.8-13
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• 2010

자운영 재배농가에 자운영 안정적 입모확보에 기초자료를 얻고자 자운영 지속재배와 매년파종시 입모 및 생육특성을 조사한 결과는 다음과 같다. 1. 자운영 지속재배포장에서 가을철 토양 속 자운영 종자는 이앙전 로타리는 토양표면에서 15 cm깊이까지 고루 분포한 반면, 부분경운 이앙시는 주로 10 cm깊이 이하에서 대부분 분포하였다. 2. 자운영 지속재배시 출아깊이는 부분경운 이앙시 1~3 cm. 로터리경운 이앙시 1~5 cm에서 출아가 되었다. 3. 자운영 지속재배는 표면에서 출아하는 매년파종 자운영보다 월동전 뿌리 길이가 깊어 월동율이 높고 논 정지 작업시 종자가 토양과 고루 혼합이 되어 입모균일도가 10.6~11.7% 가량 높아 입모수 확보가 유리하였다. 4. 자운영 지속재배시 건물생산성은 수분부족 및 저온 등기상환경이 불량 할 경우 매년파종보다 건물생산성은 높았으나 환경조건이 양호할 경우는 크게 차이가 없었다.

• 한국식품영양학회지
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• 제36권6호
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• pp.551-560
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• 2023

This study was performed to investigate antioxidant and anti-inflammatory activities of perilla(Perilla frutescens L.) seed, flower and leaf according to extraction condition. Perilla seed extracts(PSE), perilla flower extracts(PFE), perilla leaf extracts(PLE) was extracted by stirring extraction (STE, 25℃), shaking extraction (SHE, 80℃), and sonication assisted extraction(SAE, 25℃) with 94% ethanol, 60% ethanol and distilled water, followed by analysis of total polyphenol and flavonoid and testing radical scavenging activities. The highest total polyphenol content (5.47, 9.36, 38.58 mg gallic acid equivalent/g), total flavonoid content(5.77, 8.62, 46.44 mg catechin equivalent/g), ABTS(10.68, 19.46, 63.56 mg trolox equivalent/g) and DPPH(6.51, 7.69, 79.73 mg trolox equivalent/g) radical scavenging activity of PSE, PFE and PLE was observed in the HWE with 60% ethanol,. Among the three extraction method, SHE provided the best results for yield, polyphenol, flavonoid content of perilla seed, flower, leaf in comparison to STE or SAE. SHE with 60% ethanol of perilla seed, flower, leaf more effectively inhibited secretion of nitric oxide(NO) and pro-inflammatory cytokine in RAW 264.7 macrophage exposed to LPS compared to other extraction solvent and method. Therefore, these extracts obtained from perilla seed, flower, leaf could be used antioxidant and anti-inflammatory ingredients in the food industry.

• 한국작물학회지
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• 제57권4호
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• pp.317-323
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• 2012

국내 육성 유채 품종의 소포자 배양을 통한 효율적인 소포자배 생산법의 확립을 위해 '탐미유채'를 대상으로 소포자 배양 시 적당한 꽃봉오리의 크기, 고온처리시간, 배양밀도, 기내 증식조건 등의 배양조건을 구명하고자 실시한 결과, 소포자배 발생은 1핵기말과 2핵기 초기 상태의 소포자가 포함된 3.0~3.5 mm 크기의 꽃봉오리에서 채취한 소포자에서 배발생률이 가장 높았으며, 배지 1 mL 당 약 388개의 소포자 배가 발생하였다. 배양 초기에 $32.5^{\circ}C$에서 2일간 열처리하였을 때 배발생률이 가장 높았다. 소포자의 배양밀도에 따른 배발생은 $5{\times}10^4$개/mL일 때 가장 높았으며, 배양밀도가 더 이상 높아지면 배발생을 억제하였다. 발생한 소포자배는 $0.5mg{\cdot}L^{-1}$ NAA와 $1mg{\cdot}L^{-1}$ BA가 첨가된 MS 고체배지에 치상하여 배양하면 정상적인 신초로 재분화되었고, 발달한 신초를 절취하여 식물성장 조절제가 첨가되지 않은 MS고체배지에서 배양하면 뿌리가 발달하여 정상적인 식물체로 성장하였다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.318-318
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• 2017

This study was conducted to find optimum mixed sowing ratio of green manure crops to reduce the use of chemical fertilizer as well as to increase the crop yield potential which will foster the utilization of green manure crops in the upland field in view of environment friendly agriculture. According to the study, the mixed ratio, 50:50, of hairy vetch and green barley showed highest nitrogen production yield in the soil due to the relatively higher organic nitrogen supply from the hairy vetch plant as well as nitrogen fixation from the air rather than other mixed ratio. In the 50:50 mixed ratio of hairy vetch and green barely total nitrogen amount in the soil showed 17.2kg per 10a, but in the other treatment ratio such as 75:25, 25:75. total nitrogen fixation amount were 16.7, 16.9 respectively. We also conducted the experiment to compare the effect of the mixed sowing treatment of green manure crops on the production of corn cultivated as a succeeding plant of hairy vetch. According to the result, the mixed ratio, 50:50, of hairy vetch and green barley treatment showed highest yield potential of corn as 153kg per 10a in seed weight which is due to the relatively higher organic nitrogen supply from the hairy vetch plant as well as nitrogen fixation from the air rather than other mixed ratio. In the mixed sowing treatment of hairy vetch 100 and barley 0 ratio, the corn production showed 148kg per 10a which is 5kg lower than that of hairy vetch 50 and barley 50 ratio, but showed statistically no difference between those two treatment. Otherwise, different treatments, such as hairy vetch 75 and barley 25, 25 and 75, 0 and 100 showed statistically different each other. Therefore, it was concluded that green manure crops, such as hairy vetch, green barley and rye were very effective crops to increase the soil fertility and gave the positive effect to the crops to give vegetative and propagative growth condition and, in turn, increased the yield potential. We have to make policy to enhance the utility of green manure crops in the upland crops as well as faddy field for the soil fertility and crop yield production which will guarantee prominent quality of environment friendly agriculture products.

• 한국환경과학회지
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• 제28권12호
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• pp.1061-1069
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• 2019

Common reed (Phragmites australis) is widespread in reclaimed land and wetland habitats. Every year, the common reed produces extensive colonies by means of underground rhizomes and ground-surface stolons. From an agricultural point of view, the common reed's large biomass is a good material for supplying organic matter. However, it has not yet been studied in terms of seedling production, transplanting conditions, and decomposition characteristics in reclaimed land. Seeds were harvested from the native common reed in Saemangeum, South Korea, the previous year and stored on an open field. The seeds were sowed in the greenhouse at the beginning of April. Common reed decomposition was studied from June to September, with the use of coarse mesh (5 mm) stem litterbags, on three samping dates and with five replicate packs per sample. These packs were dug in five soil condition (low-salinity topsoil, subsoil, high-salinity topsoil, subsoil, paddy topsoil) to 0.2 m and 0.4 m depth. The highest germination rate of common reed seeds was observed in non-salt solution, but the exhibited germination rate was 70% at 9.38 dS m^-1. The plant height of young reed decreased steadily with increasing salinity, but leaf number did not decrease by 9.38 dS m^-1. The survival rate of the two-year-old reed was 83.3%, which was 35% higher than that of the one-year reed. The transplant success rate was 0% in the no vinyl mulching in the soil, but the first year and second year seedlings survived rates were 63% and 83.3%, respectively, in vinyl mulching. Common reed decomposition rates were faster low salinity than high salinity. All nutrient contents were found to fluctuate significantly with time by soil conditions. We also need to study the growth rate of reed transplanting seedlings by soil moisture contents and the comparison of degradation in common reed tissues.