• Korean Journal of Medicinal Crop Science
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• v.26 no.5
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• pp.401-407
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• 2018

Background: In Korea, seeds of Panax ginseng C. A. Meyer need to be stored under cold temperature and high humidity condition for months to break physiological dormancy, making storage difficult until spring-sowing. This study was conducted to test the effects of seed storage conditions and seed treatment on the emergence of seedling after spring-sowing in a nursery greenhouse. Methods and Results: After dehiscence, endocarp dried seeds in mild or completely, and wet seeds were stored in $2^{\circ}C$ and $-3.5^{\circ}C$ during winter. Storage at $-3.5^{\circ}C$ resulted in a lower emergence rate (ER) than that at $2^{\circ}C$, and additional cold ($2^{\circ}C$) treatment before or after storage at $-3.5^{\circ}C$ increased the ER. Endocarp dehydration prevented pre-germination at $2^{\circ}C$ storage and increased the ER of seeds stored at $-3.5^{\circ}C$. ER was also dependent on the batch of seeds. However, seed treatments before sowing had only limited effects on ER. Root loss was the main reason for damping-off; prolonged cold storage of seeds increased damping-off, as the detection of pathogens was not high. Conclusions: This study showed that storage conditions such as temperature and moisture content of seeds, affect the ER after spring-sowing and vitality of seedlings, suggesting further attention on seed control for secure seedling stands after spring-sowing.

• Korean Journal of Medicinal Crop Science
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• v.18 no.6
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• pp.416-420
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• 2010

Root yield and quality of ginseng cultured in paddy soil was low relatively compared with that of upland soil because of moisture injury in root during rainy season. Drainage class in soils generally divided into 6 classes, and it is possible to cultivate ginseng practically in imperfectly drainage class (IDC). This study carried out to select the varieties that is suitable for paddy soil, which is easy to be generated rusty-colored root and physiological-discolored leaf. Experiment plot arranged with the condition of soil humidity contents such as poorly drainage class (PDC) and imperfectly drainage class (IDC), and upland soil. Growth characteristics and root yield were investigated in four-year-old ginseng of varieties, Cheonpoong (CP), Yeonpoong (YP), Hwangsookjong (HS), and Jakyeongjong (JK). CP among four varieties showed the highest yield in IDC and CP was the lowest ratio in leaf discoloration and rusty-colored root. HS was followed by CP in the order of root yield, but it had the weakness that the ratio of rusty-colored root was high respectively.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.241-244
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• 2005

This study was carried out to compare growth characteristics, and extract and crude saponin contents of 4-year-old ginseng cultured by direct seeding and transplanting cultivation at several farms of the main producing district, Geumsan and Eumsung. Though root weight per plant of direct seeding cultivation was lower than that of transplanting cultivation, but yield of the former was higher than that of the latter owing to high rate of survival plant and Leaf Area Index. Dry matter partitioning ratio of direct seeding cultivation was high in primary root and low in secondary root because direct seeding cultivation elongated the length of primary root, while it suppressed the growth of secondary root. Ratio of rusty root was decreased in condition of direct seeding cultivation, while the contents of extract and crude saponin were lower than that of transplanting cultivation.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.104-104
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• 2017

Sorghum (Sorghum bicolor (L.) Moench.) is one of the most important crops for human and animal nutrition. Nonetheless, sorghum has a cyanogenic glucoside compound which can be degraded into hydrogen cyanide, toxic to humans and animals even with tiny amount. In consequence, breeding materials with a low cyanide level has been a top priority in sorghum breeding programs. To fulfill our long-term goal, we are screening sorghum accessions with low cyanide level, which would be an important breeding material for food safety. We collected seeds of various sorghum accessions and analyzed relevant metabolites to find useful breeding materials of sorghum accessions containing low cyanide. Fourteen wild relatives were obtained from the University of Georgia in US, a reference accession BTx623, and three local varieties from National Agrobiodiversity Center of Rural Development Administration in Korea, and one wild species from the Wild Plant Resources Seed Bank of Korea University in Korea. Sorghum plants were grown in plastic greenhouse under natural conditions. After growing, leaf samples were harvested at different developmental stages: seedling phase, vegetative phase (right before flowering), and reproductive phase (ripening). Using collected samples, quantification analysis were performed by an HPLC system for three metabolites (dhurrin, 4-hydroxybenzaldehyde, and 4-hydroxyphenylacetic acid) in sorghum plants. Prior to metabolome analysis, specific experimental condition for HPLC system was set to be able to separate three metabolites simultaneously. Under this condition, these metabolites were quantified in each accession by HPLC system. We observed that the metabolite contents were changed differently by developmental stages and accessions. We clustered these results into five groups as patterns of their contents by developmental stages. Most of accessions showed that 4-hydroxybenzaldehyde content was very high at seedling stage and decreased rapidly at vegetative phase. Interestingly, the patterns of dhurrin content were very different among clusters. However, 4-hydroxyphenylacetic acid content was maintained at low levels by developmental stages in most accessions. The results would demonstrate how dhurrin and alternative degradation pathways are differentiated in each accession.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.5
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• pp.294-299
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• 2000

Hairy vetch (Vicia villosa Roth) winter annual is very effective on reducing chemical nitrogen fertilizer for subsequent com by fixed organic green manure nitrogen fixed during hairy vetch growth. In this experiment, hairy vetch produced above-ground dry matter of 5 ton/ha, nitrogen yield 200 kgN/ha, at com planting on the average during 1997 and 1998. Changes in com yield and nitrogen uptake for two years were investigated after application of nitrogen fertilizer 0, 60, 120, 180, 240 kgN/ha on plot of winter fallow and hairy vetch green manure, respectively. Nitrogen status such as ear-leaf N%, SPAD value at silk and dough stage, and com yield decreased in proportion to reduction of nitrogen fertilizer at winter fallow, but nitrogen status and yield of com were not different among nitrogen fertilizer rate at hairy vetch green manure. Com yield (total dry matter) at 0 kgN/ha plot of hairy vetch was 22, 20 ton/ha in 1997, 1998, respectively and com could produce more dry matter 9, 13 ton/ha by hairy vetch green manure compared with winter fallow under the condition of no nitrogen fertilizer in 1997, 1998, respectively. Com yield (total dry matter) at 60kgN/ha of hairy vetch green manure was higher than that of high N fertilizer rate such as 180, 240 kgN/ha of winter fallow. Nitrogen uptake of com at plot of hairy vetch-no nitrogen fertilizer slightly decreased than at plot of hairy vetch - nitrogen fertilizer, but com absorbed more nitrogen of 141, 159 kgN/ha by hairy vetch green manure compared with winter fallow under no nitrogen fertilizer condition in 1997, 1998, respectively. Nitrogen fertilizer reduction for com by hairy vetch green manure was 149, 161kgN/ha in 1997, 1998, respectively. Still more, com could absorb more soil nitrogen by nitrogen fertilizer 60kgN/ha of hairy vetch green manure than by high nitrogen fertilizer such as 180, 240 kgN/ha at winter fallow. It is concluded that nitrogen fertilizer for corn could be reduced by winter cultivation and soil incorporation of hairy vetch at com planting.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.4
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• pp.409-416
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• 1987

The object of this study was to investigate the differences in dry matter production, physiological characteristic and grain yield of soybean (Glycine max (L.) Merr.) as affected by planting dates in southern region of Korea. This experiment was conducted using four planting dates and four varieties over 3 years (1982-1984). The different responses of soybean varieties to different temperature and daylength, days from planting to flowering were ranged from 43 to 55 days when planted on May 10 long daylength and low temperature condition. Flowering duration was ranged from 12 to 27 days and 9 to 13 days for the plantings made on May 10 and July 9, respectively. Therefore, it was the shortest under the short daylength and high tempera-ture condition. It also appeared that the later planting caused the lower dry matter weight and leaf area index which resulted in lower grain yield. But grain yield was not directly correlated with the unbalance of Xink/Source Ratio and the lodging from excessive vegatative growth. The grain yield was the highest in May 30 and the lowest in July 9 planting: it was ranged from 2.26 to 2.55 t/ha in the former and 0.85 to 1.20 t/ha in the latter, respectively. The increase in yield was depended on the increase in the number of pods per plant, the number of seeds per pod and the seed weight. The distribution ratio of dry matter to leaves was the hightest at the sixth trifoliolate leaf stage and gradually declined thereafter in all planting dates, and it also appeared that the later planting, the lower the ratio of dry matter to leaves.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.3
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• pp.259-263
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• 2007

Sowing time of barley after cultivation of rice has frequently been delayed because of rainfall or some other reasons by rice cultivation. Partial tillage direct grain seeder with eight row, which had been developed for rice sowing and showed many advantages in wet field, were tested for barley sowing. After flooding during $2{\sim}3days$, plots were designed to make wet condition. Three sowing methods were tested; high ridged broadcasting, plat drill seeding and partial tillage direct grain seeding. It were impossible to sow properly even in 27% of soil water content by high ridged broadcasting, plat drill seeding but could be possible to sow normally by partial tillage direct grain seeder in 42% of soil water content as good as in 27% of soil water content. Initial growth condition after sowing in plots of partial tillage direct grain seeder were normal even in plots sown in more than 50% of soil water content. No. of spike, which was $508/m^2$, in plot of partial tillage direct grain seeder sowed at 30% soil water content was better than plat drill seeding, $404/m^2$. Yield and yield components of plot of partial tillage direct grain seeder, were higher than plot sowed by plat drill seeder in same soil water content. Partial tillage direct grain seeding can be a good sowing way for barley especially in wet condition. However, parts of seeder have to be improved for barley sowing; 1) ridged width of partial tillage direct grain seeder should be $10{\sim}20cm$ wider than 10 cm, which is necessary for drainage during barley growing season in wet paddy field. 2) sowing width of partial tillage direct grain seeder was not same with one of drill seeder which was the best width for light interception and should be shorter than 30cm.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.1
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• pp.37-71
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• 2021

Rapeseed is a typical winter crop, and its freezing stress tolerance is a major feature for winter survival. Therefore, it is important to comprehend clearly the physical and molecular mechanisms of rapeseed under freezing stress conditions. This study investigates the physical and transcriptome changes of two rapeseed lines, 'J8634-B-30' and 'EMS26', under cold acclimation and freezing temperature treatments. The proline content of 'J8634-B-30' at 5 ℃ increased 8.7-fold compared to that before treatment, and there was no significant change in that of 'EMS26' RNA-sequencing analysis revealed 5,083 differentially expressed genes (DEGs) of 'J8634-B-30' under cold acclimation condition. Among the genes, 2,784 (54.8%) were up-regulated and 2,299 (45.2%) were down-regulated. The DEGs of 'EMS26' under cold acclimation condition were 5,831 genes, and contained 2,199 up-regulated genes (37.7%) and 3,632 down-regulated genes (62.3%). Among them, only DEGs annotated in the cold response-related signaling pathways were selected, and their expression in the two rapeseed lines was compared. Comparative DEGs analysis indicated that cold response related signaling pathways are proline metabolism and ABA (Abscisic acid) signaling. And ICE (Inducer of CBF expression) - CBF (C-repeat-binding factor) - COR (Cold-regulated) signaling were the significantly differentially expressed transcripts in the two rapeseed lines. The major induced transcripts of 'J8634-B-30' induced P5CS (Δ'-pyrroline-5-carboxylate synthetase), which is related to proline biosynthesis, PYL (pyrabactin resistance-like protein, ABA receptor) and COR413 (cold-regulated 413 plasma membrane 1). In conclusion, these result provide a foundation for understanding the mechanisms of freezing stress tolerance in rapeseeds. Further functional studies should be performed on the freezing stress-related genes identified in this study, which can contribute to the transgenic and molecular breeding for freezing stress tolerance in rapeseed.

• Research in Plant Disease
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• v.21 no.4
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• pp.315-320
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• 2015

Soybean mosaic virus (SMV) is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. SMV belongs to potyvirus and causes typical symptoms such as mild mosaic, mosaic and necrosis. SMV is seed-borne and also transmitted by aphid. Eleven SMV strains, G1 to G7, G5H, G6H, G7H, and G7a were reported in soybean varieties in Korea. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SML-F3/B3/FIP/BIP from coat protein gene sequence of SMV. After the reaction of RT-LAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I under daylight and UV light. Optimal reaction condition was at $58^{\circ}C$ for 60 min and the primers of RT-LAMP showed the specificity for nine SMV strains tested in this study.

• Korean Journal of Medicinal Crop Science
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• v.3 no.1
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• pp.30-34
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• 1995

Theses studies were conducted to clarify the germinating characteristics of seed treated with different temperature, light, and ($Gibberellin(GA_3)$) to produce the homogeneous seedlings Angelicogigas NAKAI. The results obtained are summarized as follows: Rate and speed of germinating at $25^{\circ}C$ were higher than $15^{\circ}C$and $20^{\circ}C$ by 87.3% and 68.9% and also the required day for germination at 25°C was shortest by 14.1 days. Germination rate at the light condition was 90.7% and that at the dark was 65.7%. Germinating ability of seed stored at $4^{\circ}C$and $-10^{\circ}C$ for 165 days as dried states was 92.0% and 91.3%, while that at room temperature was 50.3%, but the longer storage of seed than 165 days decreased the ability. Gibberellin treatment by 10ppm to seed stored at $4^{\circ}C$ and by 6ppm to seed stored at $-10^{\circ}C$ increased the germination rate more by 20.9% and 31.7% than nontreated seed.