• Molecules and Cells
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• 제38권5호
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• pp.441-451
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• 2015

Recognition of cytosolic DNA initiates a series of innate immune responses by inducing IFN-I production and subsequent triggering JAK1-STAT1 signaling which plays critical roles in the pathogenesis of infection, inflammation and autoimmune diseases through promoting B cell activation and antibody responses. The stimulator of interferon genes protein (STING) has been demonstrated to be a critical hub of type I IFN induction in cytosolic DNA-sensing pathways. However, it still remains unknown whether cytosolic DNA can directly activate the JAK1-STAT1 signaling or not. And the role of STING is also unclear in this response. In the present study, we found that dsDNA directly triggered the JAK1-STAT1 signaling by inducing phosphorylation of the Lyn kinase. Moreover, this response is not dependent on type I IFN receptors. Interestingly, STING could inhibit dsDNA-triggered activation of JAK1-STAT1 signaling by inducing SHP-1 and SHP-2 phosphorylation. In addition, compared with normal B cells, the expression of STING was significantly lower and the phosphorylation level of JAK1 was significantly higher in B cells from MRL/lpr lupus-prone mice, highlighting the close association between STING low-expression and JAK1-STAT1 signaling activation in B cells in autoimmune diseases. Our data provide a molecular insight into the novel role of STING in dsDNA-mediated inflammatory disorders.

• Steel and Composite Structures
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• 제14권3호
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• pp.295-312
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• 2013

In this paper, a simplified approach based on critical temperature for fire resistance design of steel-concrete composite beams is proposed. The method for determining the critical temperature and fire protection of the composite beams is developed on the basis of load-bearing limit state method employed in current Chinese Technical Code for Fire safety of Steel Structure in Buildings. Parameters affecting the critical temperature of the composite beams are analysed. The results show that at a definite load level, section shape of steel beams, material properties, effective width of concrete slab and concrete property model have little influence on the critical temperature of composite beams. However, the fire duration and depth of concrete slab have significant influence on the critical temperature. The critical temperatures for commonly used composite beams, at various depth of concrete and fire duration, are given to provide a reference for engineers. The validity of the practical approach for predicting the critical temperature of the composite beams is conducted by comparing the prediction of a composite beam with the results from some fire design codes and full scale fire resistance tests on the composite beam.

• 한국지반공학회논문집
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• 제23권3호
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• pp.123-131
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• 2007

일련의 CID 삼축압축시험과 실내콘관입시험을 통하여 부산모래에서의 상태정수와 정규화된 콘저항치의 상관관계를 파악하였다. 삼축압축시험결과를 토대로 부산모래의 한계상태선을 도출하였으며 3개의 한계상태정수는 $M=1.39(\phi_{cs}=34^{\circ}),\;\Gamma=1.07$ 및 $\lambda=0.068$인 것으로 산출되었다. 부산모래의 상태정수와 정규화된 콘저항치의 상관관계는 $(q_c-p)/p'=27.6\exp(-10.9\Psi)$의 식으로 표현되었으며, 이는 응력이력에 관계없이 동일함을 확인하였다. 문헌조사를 통해 얻은 다른 모래시료의 정규화된 콘저항치 기울기 m 및 $\Psi=0$에서의 절편값 $\kappa$를 비교 분석한 결과, 부산모래는 유사한 $\lambda_{ss}$ 값을 갖는 모래시료 중 가장 큰 $\kappa$값을 보였으나, m과 $\kappa$가 $\lambda_{ss}$와 갖는 관계는 기존에 발표된 자료와 일치함을 확인하였다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Dietary and Medicinal Antimutgens and Anticarcinogens
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• pp.48-49
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• 2001

The carcinogens 2-amino-3-methylimidazo［4, 5-f］quinoline (IQ) and 1, 2-dimethylhydrazine (DMH) induce colon tumors in the Fisher 344 rat that contain mutations in Ctnnbl, the gene for b-catenin, but the pattern of mutation differs from that found in human colon cancers. In both species, mutations affect the glycogen synthase kinase 3$\beta$ (GSK-3$\beta$) consensus region of $\beta$-catenin, but whereas they directly substitute critical Ser/Thr phosphorylation sites in human colon cancers, the majority of mutations cluster around Ser$_{33}$ in the rat tumors.(omitted)d)

• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Effects of Edible Phytochemicals and Their Synthetic Derivatives on Carcinogenesis and Mutagenesis
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• pp.5-6
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• 2001

The carcinogens 2-arnioo-3-methylimidazo［4,5-f］quinoline (IQ) and 1,2-dimethylhydrazine (DMH) induce colon tumors in the Fisher 344 rat that contain mutations in Ctnnb1, the gene for b-catenin, but the pattern of mutation differs from that found in human colon cancers. in both species, mutations affect the glycogen synthase kinase 3$\beta$ (GSK-3$\beta$) consensus region of $\beta$-catenin, but whereas they directly substitute critical Ser/Thr phosphorylation sites in human colon cancers, the majority of mutations cluster around Ser$^{33}$ in the rat tumors.(omitted)

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.3003-3008
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• 2007

Critical nozzle has been frequently employed to measure the flow rate of various gases, but hydrogen gas, especially being at high-pressure condition, was not nearly dealt with the critical nozzle due to treatment danger. According to a few experimental data obtained recently, it was reported that the discharge coefficient of hydrogen gas through the critical nozzle exceeds unity in a specific range of Reynolds number. No detailed explanation on such an unreasonable value was made, but it was vaguely inferred as real gas effects. For the purpose of practical use of high-pressure hydrogen gas, systematic research is required to clarify the critical nozzle flow of high-pressure hydrogen gas. In the present study, a computational fluid dynamics(CFD) method has been applied to predict the critical nozzle flow of high-pressure hydrogen gas. Redlich-Kwong equation of state that take account for the forces and volume of molecules of hydrogen gas were incorporated into the axisymmetric, compressible Navier-Stokes equations. A fully implicit finite volume scheme was used to numerically solve the governing equations. The computational results were validated with some experimental data available. The results show that the coefficient of discharge coefficient is mainly influenced by the compressibility factor and the specific heat ratio, which appear more remarkable as the inlet total pressure of hydrogen gas increases.

• 한국자기학회지
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• 제2권3호
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• pp.228-232
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• 1992

고온초전도체 $Y_1Ba_2Cu_3O_{7-y}$의 grain을 높은 자기장에서 정렬시켜 여러가지 자기적 특성을 조사하였다. Grain이 정렬된 초전도체는 단결정과 같이 Cu-O layer에 수직, 수평 방향에 대해 자기이방성을 가진다. 2~77 K의 온도영역에서 측정한 lower critical field$(H_{c1})$은 온도가 증가하면서 선형적으로 감소하였는데, Cu-O layer에 대하여 외부 자기장이 수직인 경우가 더욱 급격히 감소하였다. 온도변화에 따른 자율화$(4{\pi}\;X)$의 측정으로부터 극저온의 자화율이 -1에 가까운 값을 가진다는 것을 알 수 있었다. 또한 이 자화 곡선을 London의 공식과 two-fluid model을 이용해 최소자승법으로 fitting하여 절대 영도에서 침투깊이$({\lambda}_0)$를 구하였다. 또한 2 K에서 외부 자기장에 따른 자화곡선을 측정하였으며, Bean의 critical state model을 이용한 임계전류밀도$(J_c)$의 계산으로부터 grain내부는 단결정 수준의 높은 $(J_c)$를 가지고 있음을 알 수 있었다.

• Structural Engineering and Mechanics
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• 제49권2호
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• pp.203-223
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• 2014

The extensive use of prestressed reinforced concrete (PSC) highway bridges in marine environment drastically increases the sensitivity to both fatigue-and corrosion-induced damage of their critical structural components during their service lives. Within this scenario, an integrated method that is capable of evaluating the fatigue reliability, identifying a condition-based maintenance, and predicting the remaining service life of its critical components is therefore needed. To accomplish this goal, a procedure for fatigue reliability prediction of PSC highway bridges is proposed in the present study. Vehicle-bridge coupling vibration analysis is performed for obtaining the equivalent moment ranges of critical section of bridges under typical fatigue truck models. Three-dimensional nonlinear mathematical models of fatigue trucks are simplified as an eleven-degree-of-freedom system. Road surface roughness is simulated as zero-mean stationary Gaussian random processes using the trigonometric series method. The time-dependent stress-concentration factors of reinforcing bars and prestressing tendons are accounted for more accurate stress ranges determination. The limit state functions are constructed according to the Miner's linear damage rule, the time-dependent S-N curves of prestressing tendons and the site-specific stress cycle prediction. The effectiveness of the methodology framework is demonstrated to a T-type simple supported multi-girder bridge for fatigue reliability evaluation.

• Wind and Structures
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• 제25권5호
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• pp.415-432
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• 2017

The nonlinear aerodynamic instability of a tensioned plane orthotropic membrane structure is theoretically investigated in this paper. The interaction governing equation of wind-structure coupling is established by the Von $K\acute{a}rm\acute{a}n's$ large amplitude theory and the D'Alembert's principle. The aerodynamic force is determined by the potential flow theory of fluid mechanics and the thin airfoil theory of aerodynamics. Then the interaction governing equation is transformed into a second order nonlinear differential equation with constant coefficients by the Bubnov-Galerkin method. The critical wind velocity is obtained by judging the stability of the second order nonlinear differential equation. From the analysis of examples, we can conclude that it's of great significance to consider the orthotropy and geometrical nonlinearity to prevent the aerodynamic instability of plane membrane structures; we should comprehensively consider the effects of various factors on the design of plane membrane structures; and the formula of critical wind velocity obtained in this paper provides a more accurate theoretical solution for the aerodynamic stability of the plane membrane structures than the previous studies.

• Corrosion Science and Technology
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• 제10권1호
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• pp.1-5
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• 2011

The major purpose of the present study is to evaluate the performance of various galvanized (GI) or galvannealed (GA) mild steels and AHSS in stamping applications. Finite Element Analysis (FEA) of selected stamping operations was conducted to estimate the critical pressure boundary conditions that exist in practice. Using this information, laboratory tribotests, e.g. Twist Compression (TCT), Deep Drawing (DDT) and Strip Drawing (SDT) Tests, were developed to evaluate the performance of selected lubricants and die materials/coatings in forming galvanized steels of interest. The sheet materials investigated included mild steels and AHSS (e.g. DP600 GI/GA, DP780 GI/GA, TRIP780 GA and DP980 GI/GA). Experimental results showed that galvanized material resulted in more galling, while galvannealed material showed more powdering and flaking. The surface roughness and chemical composition of galvanized sheet materials affected the severity of galling under the same testing conditions, i.e. lubricants and die materials/coatings. The results of this study helped to determine the critical interface pressure that initiates lubricant failure and galling in stamping selected galvanized sheet materials. Thus, to prevent or postpone the critical interface conditions, the results of this study can be used to select the optimum combination of galvanized sheet, die material, die coating and lubricant for forming structural automotive components.