• 제목/요약/키워드: CpG

검색결과 921건 처리시간 0.026초

Influences of Cathodic Protection and Coating Properties on the Corrosion Control of Metallic Structure in Extremely Acidic Fluids

  • Chang, H.Y.;Yoo, Y.R.;Jin, T.E.;Kim, Y.S.
    • Corrosion Science and Technology
    • /
    • 제4권6호
    • /
    • pp.242-249
    • /
    • 2005
  • A lot of parts in FGD (Flue Gas Desulfurization) systems of fossil-fuel power plants show the environments in which are highly changeable and extremely acidic corrosive medium according to time and locations, e.g. in duct works, coolers and re-heaters etc. These conditions are formed when system materials are immersed in fluid that flows on them or when exhausted gas is condensed into thin layered acidic medium to contact materials of the system walls and roofs. These environments make troublesome corrosion and air pollution problems that are occurred from the leakage of the condensed solution. To cathodically protect the metallic structures in extremely acidic fluid, the properties of the protective coatings on the metal surface were very important, and epoxy Novolac coating was applied in this work. On the base of acid immersion tests, hot sulfuric acid decreased the hardness of the coatings and reduced greatly the content of $Na_2O$, $Al_2O_3$, and $SiO_2$ among the main components of the coating. A special kind of CP(Cathodic Protection) system has been developed and tested in a real scale of the FGD facility. Applied coating for this CP system was peeled off and cracked in some parts of the facility. However, the exposed metal surface to extremely acidic fluid by the failure of the coatings was successfully protected by the new CP system.

Immobilization of oxidative enzymes onto Cu-activated zeolite to catalyze 4-chlorophenol decomposition

  • Zol, Muhamad Najmi Bin;Shuhaimi, Muhammad Firdaus Bin;Yu, Jimin;Lim, Yejee;Choe, Jae Wan;Bae, Sungjun;Kim, Han S.
    • Membrane and Water Treatment
    • /
    • 제11권3호
    • /
    • pp.195-200
    • /
    • 2020
  • In this study, a biocatalyst composite was prepared by immobilizing oxidoreductases onto Cu-activated zeolite to facilitate biochemical decomposition of 4-chlorophenol (4-CP). 4-CP monooxygenase (CphC-I) was cloned from a 4-CP degrading bacterium, Pseudarthrobacter chlorophenolicus A6, and then overexpressed and purified. Type X zeolite was synthesized from non-magnetic coal fly ash using acetic acid treatment, and its surfaces were coated with copper ions via impregnation (Cu-zeolite). Then, the recombinant oxidative and reductive enzymes were immobilized onto Cu-zeolite. The enzymes were effectively immobilized onto the Cu-zeolite (79% of immobilization yield). The retained catalytic activity of CphC-I after immobilization was 0.3423 U/g-Cu-zeolite, which was 63.3% of the value of free enzymes. The results of this study suggest that copper can be used as an effective enzyme immobilization binder because it provides favorable metalhistidine binding between the enzyme and Cu-zeolite.

PHOSPHODIESTERASE 억제제 (PDE-1), SODIUM NITROPRUSSIDE, AMITRIPTYLINE, 및 CHLORPROMAZINE의 항-혈소판작용

  • 전보권;안상건;최상현;신경호;이민수;천연숙
    • 한국응용약물학회:학술대회논문집
    • /
    • 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
    • /
    • pp.286-286
    • /
    • 1994
  • Thrombin (0.25 U/ml : TB), 소-피부 collagen (200 $\mu\textrm{g}$/ml : CG), adenosine 5'-diphesphate (4,0 $\times$ $10^{-5}$M : ADP), 및 epinephrine (4,0 $\times$ 10 $^{-5}$M : EPI)의 가토-혈소판 응집과 단백인산화작용에 미치는 PDE-I (3-isobutyl-1-methylxanthine : IBMX, 및 KR 30075), amitriptyline (AP), chlorpromazine (CP), 및 sodium nitrogrosside (SNP)의 염향을 비교-검토하였다. 그 결과, KR은 2,2 $\times$ $10^{-7}$M 이하의 $IC_{50}$/에서 EPI > ADP > CG > TB 순으로 각각을 억제하였으며, SNP 보다도 강하였고; KR-30075보다 약하나 IBMX, AP, 및 CP도 각 응집재의 작용을 억제하였으며 특히 EPI에 대하여 $10^{-8}$M 이하의 $IC_{50}$/에서 유의한 억제력을 보였다. 각 응집제들은 41 kD 인산화는 유의하게 증가시키며 47 kD와 20 kD 단백인산화는 감소시켰는데; 모든 항응집성 약물이 41 kD 인산화-증가는 유의하게 억제하였다, 아울러, AP와 CP는 47 kD 단백인산화-감소에 영향을 미치지 않았으나 20 kD 단백인산화-감소는 억제하였다. PDE-I (IBMX와 KR)와 SNP는 47 kD와 20 kD 단백인산화-감소를 다소 약화시켰으며, 43 kD와 22 kD 단백인산화를 KR > IBMX > SNP순으로 유의하게 증가시켰고, KR의 22 kD 단백인산화작용은 현저하였다.

  • PDF

식이 중 아연과 단백질의 수준이 성숙쥐의 지질대사에 미치는 영향 (Effect of Different Dietary Zinc and Protein Levels on Lipid Metabolism)

  • 정명일
    • Journal of Nutrition and Health
    • /
    • 제22권1호
    • /
    • pp.9-22
    • /
    • 1989
  • The effect of different levels of Zn (0, 30, 300ppm) and protein(7, 20, 40%) in the diet upon lipid metabolism was investigated in Sprague-Dawley male rats weighting 180.54$\pm$29.08g(n=450 fed one of nine diets in a 3$\times$3 factorial design for 5 weeks. The reults obtained were summarized as following. 1) Total lipid contents in serum and liver were tended to be lower in LZn group than CZn and Hzn groups. Those of LP group were higher than CP and HP groups. 2) HDL-cholesterol and total cholesterol contents in serum were significantly affected by dietary Zinc level and were increased as dietary Zinc level increase. 3) Total cholesterol in liver and muscle were tended to be decrease as dietary Zinc level increase. Those in LP group were higher than CP and HP groups. 4) Zinc contents in plasma, liver, muscle and testis were tended to be lower in LZn group than CZn and HZn groups. 5) Protein contents in plasma and liver lower in LZn group than CZn and HZn groups when dietary protein level was 7% and 20%. Those in LP group were lower than those in CP and HP groups. 6) Cu contents in plasma, liver, muscle and testis were tended to be decreased as dietary Zinc level increase.

  • PDF

Effects of Aluminum Sulfate and Protein Levels on Broiler Performance

  • Park, I. H.;K. H. Nahm
    • 한국가금학회:학술대회논문집
    • /
    • 한국가금학회 2002년도 가을 학술발표논문집
    • /
    • pp.103-105
    • /
    • 2002
  • This study was conducted to determine how broiler performance with the level of crude protein(23 CP%, 21 CP% and 19 CP%) can be changed and affected, adding Alum(Al$_{2}$(SO$_{4}$)$_{3}$ㆍ14H$_{2}$O) and no Alum in litter(two ALUM levels and 3 Protein levels with 2$\times$3 factorial design). Alum was added as a top dressing to the litter at a rate of 200g ALUM / kg of rice bran. With the exception of the protein levels for feed :gain(P〈 0.05), there were no significant differences in feed intake, weight gain and feed :gain of chicks. For ammonia gas emission, both the main effects of Alum and protein(P〈 0.01 and 0.05) at 3weeks and interaction between Alum and protein(P〈 0.05), the main effect of Alum(P〈 0.01) at 6weeks did affected them. In litter excluded the main effects of Alum(P〈 0.05), there was a significant difference among N contents ranged from pH to the rate of C: Organic-N(P〈 0.01).

  • PDF

Growth and physiological responses of broiler chickens to diets containing raw, full-fat soybean and supplemented with a high-impact microbial protease

  • Erdaw, Mammo M.;Wu, Shubiao;Iji, Paul A.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제30권9호
    • /
    • pp.1303-1313
    • /
    • 2017
  • Objective: This study evaluated the change and function of the pancreas, and small intestine in relation to growth performance of broilers on diets supplemented with raw soybean meal (RSBM) and protease. Samples of test ingredients and diets, after mixing and prior to being used were also assessed on contents of anti-nutritional factors. Methods: A $3{\times}3$ factorial study was used, with three levels of RSBM (commercial soybean meal [SBM] was replaced by RSBM at 0, 10%, or 20%) and protease (0.1, 0.2, or 0.3 g/kg). Each treatment was replicated six times with nine birds per replicate. Birds were housed in cages, in climate-controlled room and fed starter, grower and finisher diets. Results: Levels of trypsin inhibitors in the diets, containing varying levels of RSBM ranged between 1,730.5 and 9,913.2 trypsin inhibitor units/g DM. Neither RSBM nor protease supplementation in diets significantly affected (p>0.05) the body weight of broilers in the entire periods (0 to 35-d). Increasing the level of RSBM in diets increased the weight of the pancreas at d 10 (p<0.000), d 24 (p<0.001), and d 35 (p<0.05). Increasing levels of RSBM in the diets reduced the apparent ileal digestibility of crude protein (CP), and amino acid (AA) at d 24. Increasing level of RSBM in the diets decreased (p<0.01) pancreatic protein content, but this was increased (p<0.05) when protease was added to the diets (0 to 10-d). Increasing the level of protease improved the pancreatic digestive enzymes, including trypsin (p<0.05), chymotrypsin (p<0.01), and general proteolytic enzymes (p<0.05). Conclusion: The commercial SBM could be replaced at up to 20% by RSBM for broilers. Although protease supplementation slightly improved the digestive enzymes, and the ileal digestibilities of CP and AA, the CP and AA were negatively affected by increasing RSBM.

Chemical Composition of Some Tropical Foliage Species and Their Intake and Digestibility by Goats

  • Kongmanila, Daovy;Ledin, Inger
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제22권6호
    • /
    • pp.803-811
    • /
    • 2009
  • The chemical composition and water extractable dry matter (DM) of foliages from Erythrina (Erythrina variegata), Fig (Ficus racemosa), Jackfruit (Artocarpus heterophyllus), Jujube (Ziziphus jujuba Mill), Kapok (Ceiba pentandra) and Mango (Mangifera indica) and the feed intake, digestibility and N retention when feeding these foliages were studied in two experiments. In Experiment 1, 12 male goats, 3.5 months old and weighing 14.6 kg, were randomly allocated to a diet consisting of one of the foliages in a change-over design with three periods. The foliages were offered ad libitum at the level of 130% of the average daily feed intake. The Erythrina foliage had a low content of DM and condensed tannins (CT) and a high concentration of crude protein (CP) in leaves plus petioles (193 g/kg DM) and stem, while the Mango foliage had a low CP (69 g/kg DM) and high DM content. The other foliages were intermediate. High content of CT was found in the leaves plus petioles of Jackfruit foliage and in the stem of Fig and Mango foliage. There was a difference in feed intake, nutrient intake, apparent digestibility and N retention between the foliages, with Erythrina, Jackfruit and Kapok foliage being significantly higher in these parameters than Fig, Jujube and Mango foliage. The water extractable DM could be used to estimate N retention, but not DM digestibility in this study. In Experiment 2, 4 male goats weighing 13.4 kg and 6 months old were allocated to a 4${\times}$4 Latin square design. The treatments were: water spinach ad libitum and Fig, Jujube or Mango foliage ad libitum +0.5% of BW as water spinach DM. Feed intake, apparent digestibility and N retention were not significantly different among the foliage diets, but higher than for water spinach alone (p<0.05). Supplementation with water spinach to a diet consisting of low quality foliages such as Fig, Jujube and Mango, increased DM and CP intake, apparent digestibility and N retention, compared to feeding these foliages as sole feeds.

Iodine Supplementation of Leucaena leucocephala Diet for Goats. I. Effects on Nutrient Utilization

  • Rajendran, D.;Pattanaik, A.K.;Khan, S.A.;Bedi, S.P.S.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제14권6호
    • /
    • pp.785-790
    • /
    • 2001
  • Twelve indigenous male goats, comprising of six intact and six castrated (2.5-3 years; $24.4{\pm}0.62kg$) were assigned evenly into two dietary treatments, viz. $I_0$ and $I_{100}$ and were used to study the effect of supplementation of iodine on the nutrient utilization when their diet contained Leucaena leaf meal. They were offered a conventional concentrate mixture along with Leucaena leucocephala leaf meal, the latter to meet 50% of their crude protein (CP) requirements, and supplemented with either no iodine ($I_0$) or 0.1 mg of iodine ($I_{100}$)/day/animal as potassium iodide for a period of 105 days. Wheat straw given ad libitum was the sole source of roughage. A metabolism trial of 8 days duration was conducted after 90 days of experimental feeding. It was observed that the overall dry matter (DM) intake during experimental period was higher (p<0.05) in $I_{100}$ group as compared to $I_0$ group (508.6 vs. $443.7g\;d^{-1}$). The intake of CP, digestible crude protein (DCP) and metabolisable energy (ME), although non-significant, tended to be higher in the iodine supplemented group, $I_{100}$. Digestibility of dry matter, organic matter (OM), CP, ether extract and crude fiber (CF) did not differ (p>0.05) between the treatments. However, nitrogen retention was higher (p<0.01) in $I_{100}$ than $I_0$ with the values being 2.63 and $1.70g\;d^{-1}$, respectively. No difference (p>0.05) was evident in the retention of calcium and phosphorus between the two groups. The castrated animal exhibited lower DM intake concurrent with higher digestibility of DM and crude fibre (p<0.05), and organic matter and total carbohydrates (p<0.01) when compared to intact ones. It was concluded that supplementation of iodine to leucaena based ration may help in improving the DM intake and nitrogen utilization by goats.

고려홍삼으로부터 분리한 compound K 함유분획에 의한 대식세포의 toll-like receptor-의존성 신호전달로 활성조절 분석 (Compound K (CK) Rich Fractions from Korean Red Ginseng Inhibit Toll-like Receptor (TLR) 4- or TLR9-mediated Mitogen-activated Protein Kinases Activation and Pro-inflammatory Responses in Murine Macrophages)

  • 양철수;고성룡;조병구;이지연;김기혜;신동민;육재민;손현수;김영숙;위재준;도재호;조은경
    • Journal of Ginseng Research
    • /
    • 제31권4호
    • /
    • pp.181-190
    • /
    • 2007
  • 본 연구에서는 고려홍삼으로부터 새로 분리한 CK 함유분획을 이용하여 마우스 대식세포에 대한 선천면역반응 조절에 미치는 영향을 조사하였다. 본 연구에서 사용된 농도의 CK 함유분획에서는 세포독성 효과가 관찰되지 않았으며 CK함유 분획의 전처리에 의하여 그람음성세균의 LPS, 또는 CpG-ODN에 의해 유도되는 NF-${\kappa}B$와 MAPK 활성 및 전염증성 사이토카인, NO의 분비가 TLR4 및 TLR9 특이적으로 억제되었다. 이와 같은 결과는 CK 함유분획이 TLR4을 매개로하는 염증반응뿐만 아니라 TLR9을 통한 염증반응에도 영향을 미치는 것으로 해석된다. 따라서 앞으로 CK 함유 분획에 포함된 개별 사포닌 등 시료 성분에 대한 면밀한 분석, 그리고 이들 개별 물질이 각각의 신호전달 체계에 미치는 영향과 그 기작에 대한 연구가 더욱 필요할 것으로 사료되며 염증억제제로서의 개발 가능성을 탐구하기 위한 생체 내에서의 효능 및 작용기전 분석이 요구된다.

Comparative Calorimetric Evaluation of Ammoniated Straw-Based Rations Supplemented with Low Levels of Untreated and Formaldehyde Treated Groundnut Cake and Fish Meal with Respect to Growing Buffalo Calves

  • Tiwari, C.M.;Jadhao, S.B.;Chandramoni, Chandramoni;Murarilal, Murarilal;Khan, M.Y.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제13권6호
    • /
    • pp.761-773
    • /
    • 2000
  • Eighteen growing male Murrah buffalo (Bubalus bubalis) calves were divided into three groups consisting of six animals each and fed three urea ammoniated wheat straw (UAS) -based rations supplemented with concentrate mixtures (roughage: concentrate ratio 58:42) containing deoiled ground nut cake, GNC (8%), formaldehyde treated GNC (8%) or fish meal (8%) to undertake comparative evaluation of these rations in terms of their $CH_4$ production and growth (285 d duration) potential. A digestibility trial (10 d duration) was followed by a comparative calorimetric study in respiration chamber. Dry matter (DM) intake (84.3 to $89.3g/kg\;W^{0.75}d^{-1}$) did not differ between treatments. The digestibility coefficient of DM, organic matter (OM), crude protein (CP), neutral and acid detergent fiber did not differ significantly in different diets. Urinary energy loss as a percent of gross energy (GE) was not affected by diets. Average values of $CH_4$ production were 84.3, 77.6 and 99.1 g/d and $CH_4$ energy losses as percent of gross energy were 5.7, 5.2 and 6.1 percent on .GNC, formaldehyde treated GNC and fishmeal, respectively, and did not differ significantly. When expressed per unit of digestible OM intake, $CH_4$ production (g) was lower (p<0.05) on formaldehyde treated GNC (30.6) than on untreated GNC (30.6) and fish meal (31.9). Total ME intake and heat production were similar and hence the energy balances on different diets were similar. Nutritive value of rations in terms of digestible CP and ME were similar. Average daily gain calculated on the basis of regression of fortnights on cumulative liveweight gain in calves fed on concentrate containing unprotected GNC, protected GNC and fish meal were 437.1, 483.9 and 481.6 g, respectively. This indicated that the intake of energy was sufficient to meet the requirement of calves growing at 400 g per d. However, CP intake was around 150% of the stipulated standard (Kearl, 1982). Feed conversion ratios on unprotected GNC, protected GNC and fish meal were 11.60, 11.10 and 10.4 respectively. It was concluded that because significantly (p<0.05) low $CH_4$ is produced on protected GNC (8%), it is very good and sustainable protein source in comparison to poor quality fish meal and untreated GNC to be used in concentrate mixture for supplementing UAS-based diets.