• Asian-Australasian Journal of Animal Sciences
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• 제24권1호
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• pp.65-72
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• 2011

Twenty-four Holstein dairy cows were used to evaluate the single and combined effects of different levels of crude protein (CP) and monensin treatment during early lactation on blood metabolites, milk yield and digestion of dairy cows. The experiment was designed as a completely randomized block with a $3{\times}2$ factorial arrangement of treatments. The factors were three concentrations of CP supplement (19.5, 21.4, and 23.4% of dry matter) and two levels of monensin (0 and 350 mg per cow per day). The experiment consisted of three phases and each phase was 3 wk in length. Monensin did not affect milk yield, lactose, solids-non-fat (SNF), blood glucose, triglyceride and DMI, but increased blood cholesterol, blood urea nitrogen (BUN), insulin and reduced blood ${\beta}$-hydroxybutyrate (BHBA), milk fat and protein percentage. Monensin premix significantly decreased rumen ammonia, but rumen pH and microbial protein synthesis were not affected by monensin treatment. Increasing dietary CP improved milk and protein production, but did not alter the other components of milk. Digestibility of NDF, ADF, CP were improved by increasing dietary CP. Increasing dietary CP from 19.5 to 21.4% had no significant effect on ruminal ammonia, but increasing CP to 23.4% significantly increased ruminal ammonia. There was a linear relationship between level of crude protein in the diet and volume of urine excretion. Microbial protein synthesis was affected by increasing CP level; in this way maximum protein synthesis was achieved at 23.4% CP.

• Asian-Australasian Journal of Animal Sciences
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• 제3권1호
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• pp.21-25
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• 1990

Fructo-oligosaccharides are found in many plants, such as onion, burdock and wheat. They are not well hydrolyzed by digestive enzymes in animals, but are peculiarly assimilated by Bifidobacterium and some useful bacteria. In our previous experiment (Kobayashi et al., 1987) it was suggested that they were effective in decreasing energy loss in the metabolism of dairy calves. In the present study, the effects of fructo-oligosaccharides on body weight, milk-yield and milk-components (fat, protein and solids-not-fat) were investigated in dairy cows. Lactating cows were fed a standard diet containing fructoligo saccharides at 18.70g, 9.35g and 0.0g (control) per 100kg body weight, day for three weeks. Neither treatments significantly affected any of the parameters examined. The fructo-oligosaccharides were assumed to be hydrolyzed by rumen microorganisms and hardly to affect the bacterium florae in the intestines of the lactating cows.

• 한국임상수의학회지
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• 제19권2호
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• pp.228-235
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• 2002

유방염은 젖소에 있어서 유량의 감소, 유질 저하로 인한 우유 폐기문제, 젖소의 도태, 치료비 및 노동력의 부담등을 초래하는 가장 경제적 손실이 큰 질환이다. 현재까지 비유기 최종 착유 후에 항생제를 주입하는 건유기 치료법이 유방염 관리에 가장 효과적이며 폭넓게 쓰이고 있는 방법으로 알려져 있다. 하지만, 건유기 치료는 건유기 주입 항생제 제품들이 건유기 초기에만 지속적인 활성을 지니기 때문에 건유기 말기와 분만 전기에 있어서의 신규 감염은 방어할 수 없다. 따라서 본 연구는 건유기 유방염 관리를 위해 PLGA를 이용한 서방형 생분해 cephalexin microsphere를 제조하여 이의 임상적 효능을 평가하고자 하였다. PLGA는 무독성의 조직 반응을 일으키지 않는 특성으로 인해 약물 방출 조절 체계의 일환으로 인정을 받아왔다. 본 연구에서 cephalexin microsphere는 표면에 특징적인 구멍을 가지고 있는 구형 모양으로 확인이 되었으며 약물 방출 시험에서 초기 과다 방출 이후로 21일간 약물의 방출이 점차 감소한 뒤 3주와 4주 사이에 2차 방출을 보이는 맥동성 방출 양상이 관찰되었다. 현장적용 시험에서는 cephalexin microsphere를 주입한 실험군에서 대조군에 비해 분만 후 신규 감염율 및 치료율, 평균 체세포 수의 변화의 측면에서 볼 때 유의성 있는 차이가 있음을 확인하였다. 따라서 생분해 microsphere를 이용한 건유기 치료법은 건유기 동안 신규 감염을 예방하고 기감염을 감소시킴으로써 종전의 건유기 치료를 좀 더 효과적으로 증진시킬 수 있을 것으로 사료된다.

• 한국컴퓨터정보학회논문지
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• 제26권10호
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• pp.139-145
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• 2021

본 논문에서는 소의 사료 취식량(Feed Intake), 반추(Rumination), 발정기(In Heat) 모니터링 기술을 이용하여, RFI(Residual Feed Intake) 모니터링 및 신진대상 이상을 예측하는 웨어러블 디바이스 및 PC용 웹과 스마트폰 어플리케이션을 이용한 모니터링 시스템을 설계하고 구현하였다. 본 시스템의 개발로 농장주는 경제적 효율성의 증가가 기대된다. 사료 섭취량을 분석하면, 소의 체중에 근거한 추천 사료량과 소가 섭취하는 사료량과의 차이를 확인할 수 있으며, Metabolic disorder(신진대사 이상)에 대한 조기 발견이 가능할 것으로 예상된다. 본 논문의 결과물을 사용하는 농장주는 가장 효율적인 성과를 나타내는 소를 구별할 수 있으며, 소의 표피(목)에 부착하는 웨어러블 장치로부터 입력되는 6축 모션 센서 신호와 웨어러블 장치에 부착된 마이크를 통해 입력되는 소의 목넘김 소리를 통해서 소의 반추와 사료섭취량을 측정할 수 있다. 향후에는 심박, 호흡 등의 추가적인 생체신호를 측정할 수 있도록 개선 작업을 진행할 예정이다.

• 한국축산식품학회지
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• 제37권4호
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• pp.606-616
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• 2017

The objective of this research was to investigate the effects microbial transglutaminase (mTGs) on the physicochemical, microbial and sensory properties of kefir produced by using mix cow and soymilk. Kefir batches were prepared using 0, 0.5, 1 and 1.5 Units m-TGs for per g of milk protein. Adding m-TGs to milk caused an increase in the pH and viscosity and caused a decrease in titratable acidity and syneresis in the kefir samples. Total bacteria, lactobacilli and streptococci counts decreased, while yeast counts increased in all the samples during storage. Alcohols and acids compounds have increased in all the samples except in the control samples, while carbonyl compounds have decreased in all the samples during storage (1-30 d). The differences in the percentage of alcohols, carbonyl compounds and acids in total volatiles on the 1st and the 30th d of storage were observed at 8.47-23.52%, 6.94-25.46% and 59.64-63.69%, respectively. The consumer evaluation of the kefir samples showed that greater levels of acceptability were found for samples which had been added 1.5 U m-TGs for per g of milk protein.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제21권3호
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• pp.155-162
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• 2018

Infant formula is classified into standard cow's milk-based and special formulas. This review aimed at summarizing the types of special milk formulas currently sold in Korea, and the appropriate indications for the use of these formulas; lactose free formula, soy-based formula, protein hydrolysate formula, amino acid-based formula, preterm formula, medium chain triglyceride formula, low-phosphorus formula, protein-energy-enriched formula, and formulas for inborn errors of metabolism.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1247-1247
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• 2001

Constituents of animal biofluids such as milk, blood and urine contain information specifically related to metabolic and health status of the ruminant animals. Some changes in composition of biofluids can be attributed to disease response of the animals. Mastitis is a major problem for the global dairy industry and causes substantial economic losses from decreasing milk production and reducing milk quality. The purpose of this study was to investigate potential of NIRS combined with multivariate analysis for cow's mastitis diagnosis based on NIR spectra of milk, blood and urine. A total of 112 bulk milk, urine and blood samples from 4 Holstein cows were analyzed. The milk samples were collected from morning milking. The urine samples were collected before morning milking and stored at -35$^{\circ}C$ until spectral analysis. The blood samples were collected before morning milking using a catheter inserted into the carotid vein. Heparin was added to blood samples to prevent coagulation. All milk samples were analyzed for somatic cell count (SCC). The SCC content in milk was used as indicator of mastitis and as quantitative parameter for respective urine and blood samples collected at same time. NIR spectra of blood and milk samples were obtained by InfraAlyzer 500 spectrophotometer, using a transflectance mode. NIR spectra of urine samples were obtained by NIR System 6500 spectrophotometer, using 1 mm sample thickness. All samples were divided into calibration set and test set. Class variable was assigned for each sample as follow: healthy (class 1) and mastitic (class 2), based on milk SCC content. SIMCA was implemented to create models of the respective classes based on NIR spectra of milk, blood or urine. For the calibration set of samples, SIMCA models (model for samples from healthy cows and model for samples from mastitic cows), correctly classified from 97.33 to 98.67% of milk samples, from 97.33 to 98.61% of urine samples and from 96.00 to 94.67% of blood samples. From samples in the test set, the percent of correctly classified samples varied from 70.27 to 89.19, depending mainly on spectral data pretreatment. The best results for all data sets were obtained when first derivative spectral data pretreatment was used. The incorrect classified samples were 5 from milk samples,5 and 4 from urine and blood samples, respectively. The analysis of changes in the loading of first PC factor for group of samples from healthy cows and group of samples from mastitic cows showed, that separation between classes was indirect and based on influence of mastitis on the milk, blood and urine components. Results from the present investigation showed that the changes that occur when a cow gets mastitis influence her milk, urine and blood spectra in a specific way. SIMCA allowed extraction of available spectral information from the milk, urine and blood spectra connected with mastitis. The obtained results could be used for development of a new method for mastitis detection.

• Neonatal Medicine
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• 제15권1호
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• pp.75-79
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• 2008

목 적 : 혈변을 보였으나 확진 할 수 없었던 4개월 이하의 영아에서 내원 당시의 임상 상태 및 2년 후의 추적관찰을 통해 얻은 정보로 임상 소견 및 향후 알레르기 질환의 발현 여부에 대하여 알아보고 치료 방침 설정에 도움이 되고자 본 연구를 시행하였다. 방 법 : 신생아와 조기 영아기에 혈변을 보았던 환아들 중 감염 및 수술적 질환을 제외한 환아의 임상소견 및 2년 후의 추적관찰을 시행하였다. 결 과 : 모든 환아에서 RAST 우유 검사는 음성이었고, 환아들 모두는 자연 호전 되었으며, 2년 후 추적관찰 결과 예후는 양호하였다. 내원 당시의 총 호산구수 증가 유무에 따른 2년 후의 알레르기 성향에서 차이를 보이지 않았다. 결 론 : 신생아와 조기 영아기에 혈변을 보았던 환아들 중 감염 및 수술적 질환을 제외한 환아의 임상양상 및 예후는 모두 양호하였고, 특별한 치료 없이 모두 호전되었으므로 불필요한 치료를 배제할 수 있을 것이다.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제25권3호
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• pp.263-275
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• 2022

Purpose: The present international survey among healthcare providers aimed to collect data on theoretical knowledge and clinical practices in the diagnosis and management of cow's milk protein allergy (CMPA) and lactose intolerance (LI) in infants. Methods: A global survey was conducted in several countries with diverse health care settings. The survey consisted of multiple-choice questions in 3 main domains: (1) understanding and clinical practices around CMPA and LI; (2) case scenarios; and (3) disease-specific knowledge and potential educational needs. Results: Responses were available from 1,663 participants. About 62% of respondents were general practitioners or general pediatricians, and the remainder were pediatric allergists/gastroenterologists (18%) or other health practitioners (20%). The survey identified knowledge gaps regarding the types of CMPA (IgE-mediated vs. non-IgE-mediated) and the clinical overlap with LI. The survey suggested diverse clinical practices regarding the use of hypoallergenic formulas, as well as misconceptions about the prebiotic benefits of lactose in extensively hydrolyzed formulas in non-breastfed infants with CMPA. Responses to the two case scenarios highlighted varying levels of awareness of the relevant clinical practice guidelines. While respondents generally felt confident in managing infants with CMPA and LI, about 80% expressed an interest for further training in this area. Conclusion: The current survey identified some knowledge gaps and regional differences in the management of infants with CMPA or LI. Local educational activities among general and pediatric healthcare providers may increase the awareness of clinical practice guidelines for the diagnosis and treatment of both conditions and help improve clinical outcomes.

• 한국초지조사료학회지
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• 제36권2호
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• pp.98-103
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• 2016

전라북도 고창 조사 지역 내 유기 낙농 농가 수는 연도가 지남에 따라 증가하지 않았지만, 전체적인 사육규모와 사육두수는 증가하였다. 연 평균 유량은 국내 검정 농가 평균에 비해 유기 낙농가가 최대 9 kg/d가 낮았지만 유지방과 유단백 함량에서는 큰 차이를 보이지 않았다. NRC (2001)의 사양표준 프로그램을 이용하여 영양소 공급 수준을 평가한 결과, 급여 사료의 에너지 비율 특히 비구조탄수화물의 비율을 감소시킬 필요가 있으며, 또한 대사단백질 및 섬유소 비율을 증가시켜 전체적인 영양소 공급 수준을 조정한다면 착유우의 건강상태 및 유생산성이 개선될 것으로 생각된다.