• Journal of Animal Science and Technology
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• 제49권3호
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• pp.351-358
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• 2007

본 연구는 국내에서 사용되고 있는 몇 가지 단백질 원료사료를 사용하여 단백질 fraction과 in situ 단백질 분해율을 구한 다음 이들 사이에서의 상관관계를 살펴보고자 실시하였다. 원료사료는 대두박, 콘글루텐, 면실박, 카폭박 및 임자박이었다. 단백질 fraction은 CNCPS에서 제시하는 방법으로 구하였으며, in situ 단백질 분해율은 캐뉼라가 장착된 홀스타인 거세우 3두를 이용하여 반추위에서 원료사료를 4, 8, 12 및 24시간 배양하여 구하였다. 단백질 fraction 중 A fraction은 카폭박이 14.6%로 가장 높았고, 콘글루텐이 0.6%로 가장 낮았다(P<0.05). B1 fraction은 대두박이 8.27%로 가장 높았으며, B2 fraction은 대두박과 면실박이 74%로 가장 높았다. B3 fraction은 임자박이 40%로 다른 원료사료에 비교해 뚜렷하게 높았다. C fraction은 콘글루텐이 약 42.5%로 가장 높았다. In situ 조단백질 분해율은 대두박이 98%로 가장 높았고, 콘글루텐은 28%로 가장 낮았다. 단백질 fraction과 in situ 분해율 사이의 상관관계를 보면, 쉽게 용해되는 부분(A, B1 fraction vs a값) 사이에, in situ 조단백질 분해율과 소화가능한 단백질 fraction 사이에, 그리고 in situ 조단백질 분해율에서 a값을 제외한 값과 B2+B3 fraction 사이에는 상관관계가 높았다(P<0.01). 본 연구결과에 의하면, 단백질 fraction은 원료사료의 반추위내 분해율을 추정하는 데 이용될 수 있을 것으로 사료되며, 앞으로 더 정확한 평가를 위해서는 더 많은 원료사료에 대한 분석이 필요하다고 본다.

• 한국버섯학회지
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• 제20권3호
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• pp.168-172
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• 2022

느타리 재배에서 면실박을 대체하여 채종박을 첨가한 결과는 다음과 같다. 처리 간 화학성은 pH는 4.5~4.7, 총탄소함량은 46.3~46.5%, 총질소함량은 1.6~1.7%, 탄소질소비율은 27.0~27.8로 대조와 뚜렷한 차이를 보이지 않았다. 처리 간 균사생장은 채종박을 5% 첨가한 혼합배지에서 '흑타리'는 85.1 mm, '수한1호'는 72.8 mm로 가장 빨랐고, 균사밀도는 처리 간 뚜렷한 차이를 보이지 않았다. 자실체 특징은 '흑타리'의 경우 채종박이 5% 첨가된 혼합배지에서 갓 색은 L값 32.5로 대조에 비해 어두웠으며, 갓 두께 3.2 mm, 갓 직경 27.6 mm, 대 길이 86.0 mm, 대 직경 9.1 mm, 병 당 유효경수 25.9개, 병 당 수량 189.7 g으로 대조에 비해 높게 나타났다. 채종박이 10% 첨가된 혼합배지에서는 병 당 수량이 184.5 g으로 대조와 대등하게 나타났다. '수한1호'의 경우 채종박이 5% 첨가된 처리에서 갓 색은 L값 28.8로 대조에 비해 어두웠고, 갓 두께 4.5 mm, 대 직경 12.3 mm으로 대조에 비해 높게 나타났으며, 병 당 수량 145.5 g으로 대조와 대등하였다. '흑타리'와 '수한1호' 모두 채종박만 첨가된 처리에서는 병 당 수량이 대조에 비해 낮은 경향을 보였다. 따라서 느타리 재배를 위한 채종박의 첨가량은 5%가 적당하며, 5% 초과시 병 당 수량이 낮아지 경향에 대해서는 추후 면밀한 연구가 진행되어야 할 것으로 판단된다.

• 한국버섯학회지
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• 제12권4호
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• pp.367-370
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• 2014

총탄소함량은 T4를 제외한 모든 처리구에서 대등하였으며, 총질소함량은 T3에서 2.17%로 대조구에 가장 근접한 값을 나타내었다. C/N율은 T4에서 39로 다른 처리구보다 높았다. 혼합배지에 따른 자실체의 생육특성을 조사한 결과 갓직경은 T2에서 가장 컸고, 대직경은 T2와 T4에서 가장 컸고, 대길이는 T1과 T2에서 가장 많았다. 유효경수는 T1과 T3에서 가장 많았다. 혼합배지에 따른 처리구별 수량은 T3에서 171g/병으로 대조구와 대등하였고, 회수율도 89.2%로 대조구와 대등한 결과를 나타내었다.

• Asian-Australasian Journal of Animal Sciences
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• 제12권3호
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• pp.493-502
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• 1999

Majority of livestocks are kept in the tropics. Demand for animal products (meat and milk) is continuously increasing and is related to human population growth. Consequently, potential feeds should be continuously identified particularly on their nutritive values. Crop residues and agricultural by-products are the main feed sources for feeding livestock in the tropics. Their nutritive values ranging from low to medium quality level although Some agricultural by-products such as cotton seed meal and coconut meal are rich in nitrogaen contents. From literatures, nutritive values in these ingredients are mainly based on their chemical composition and to some extent based on limited number of in vivo studies. However, optimum of inclusion in the diet is suggested. Development of tree legumes should be thoroughly considered since they grow well in most tropical regions. In order to improve nutritive value of tropical feeds, biological treatments should be considered. Effect of secondary compounds decreasing efficiency of nutrient utilization in the rumen, to some extent could be reduced by introducing probiotics.

• Asian-Australasian Journal of Animal Sciences
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• 제16권9호
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• pp.1339-1347
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• 2003

An experiment was conducted to investigate the effects of microbial phytase ($Natuphos^{(R)}$) supplementation in combination with carbohydrases (composed of enzymes targeted to soybean meal (SBM) dietary components such as $\alpha$-galactosides and galactomannans; $Endo-Power^{(R)}$) to corn-soybean meal based diet (CSD) and complex diet (CD) with a partial replacement of SBM with rape seed meal (RSM) and cotton seed meal (CSM) on growth performance and nutrient digestibility of growing pigs. A total of 168 growing pigs averaging $13.18{\pm}1.77kg$ of initial body weight was arranged as a $2{\times}2$ factorial design with main effects of diet types (corn-SBM based diet (CSD) and complex diets (CD; 5% of SBM was replaced with 2.5% of RSM and 2.5% of CSM in diet for phase I (0 to 3 weeks) and 6% of SBM was replaced with 3% of RSM and 3% of CSM in diet for phase II (4 to 7 weeks))) and enzyme supplementation (none and 0.1% of phytase (500 FTU/kg diet) and 0.1% of carbohydrases). The diet with enzyme application were formulated to have a 0.18% unit lower aP than diets without enzyme application. Each treatment had three replicates with 14 pigs per replicate. To determine supplementation effect of phytase and carbohydrases on ileal amino acid digestibility of SBM, RSM and CSM, a total of 18 T-cannulated pigs (initial body weight; $13.52{\pm}1.24kg$) were assigned to six dietary treatments in the present study. Dietary treatments in metabolic trial included 1) SBM diet, 2) SBM diet+with enzymes (phytase (500 FTU/kg) and carbohydrases at 0.1%, respectively), 3) CSM diet, 4) CSM diet+enzymes, 5) RSM diet and 6) RSM diet+enzymes. During whole experimental period (0 to 7 wks), there was no difference in growth performance between diets (CSD and CD). However, dietary phytase and carbohydrases supplementation significantly improved gain/feed ratio (G:F) of growing pigs. During the phase II (4-7 weeks), dietary phytase and carbohydrases supplementation significantly improved all fecal nutrient digestibilities (Dry matter (DM), gross energy (GE), crude protein (CP), crude fat (CF), calcium (Ca) and phosphorus (P)). Dietary phytase and carbohydrases supplementation improved significantly overall ileal amino acid digestibilities of SBM, RSM and CSM based diets (p<0.05). The simultaneous inclusion of phytase and carbohydrases in both of CSD and CD reduced feed cost per kg body weight gain (FCG). Also, results suggest that 2.5 to 3% of RSM and CSM, respectively, might be used as a protein source in growing pig diets without having an adverse effect on the growth performance and nutrient digestibility and simultaneous phytase and carbohydrases addition improves nutritional value of SBM, RSM and CSM by improving ileal amino acid digestibilities.

• Asian-Australasian Journal of Animal Sciences
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• 제13권10호
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• pp.1388-1393
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• 2000

The present study was conducted to examine the feasibility of utilising bamboo (Bambusa arundinacea) shoot shell (BSS) in ruminants. Chemical composition, rumen degradability and some antinutritional compounds were determined for fresh and boiled BSSs to evaluate its feed value and safety. Thirty-two Holstein heifers were allocated to four groups and used to investigate the response in growth rate to supplementing ammoniated rice straw with fresh shell (phase 1) or silage of boiled BSS (phase 2). All animals were offered ammoniated straw ad libitum with 1kg of cotton seed meal (phase 1) or 0.5 kg of cotton seed meal and 0.5 kg of concentrate mixture (phase 2) per head per day. The BSS was supplemented at levels of 0, 3, 6 or 9 kg/d (phase 1) and 0, 5, 10 or 15 kg/d (phase 2) (as fed basis). The BSS was very high in moisture content, and its contents of crude protein and neutral detergent fiber were 13~16% DM and 65~76% DM, respectively; boiling resulting in higher moisture and protein. No hydrocyanic acid was detected in both BSSs and content of tannins was negligible. Rumen degradability of BSS was reasonably high, and with boiling the rapidly degradable fraction decreased, and potentially degradable component increased. Silage of the boiled BSS was slightly lower in both rapidly and slowly degraded fractions than the fresh BSS. Animals consumed all supplemented BSSs without any adverse health problems. Intake of ammoniated straw decreased with the increasing levels of BSS, but total intake was higher in almost all supplementary groups than in the non-BSS. Heifers had a higher growth rate in phase 1 with fresh BSS than in phase 2 with ensiled shell, and daily weight gains were 622, 629, 744 or 690 g in phase 1, and 578, 575, 677 or 635 g in phase 2 at four BSS levels, respectively. For both phases growth rate was significantly higher for the animals in groups 3 and 4 than those in groups 1 and 2 (p<0.01), with little difference between groups 1 and 2 (p>0.05) but significant difference between groups 3 and 4 (p<0.05). Supplementation with BSS also resulted in an improved feed conversion rate, with the least concentrate consumption in group 3 for both phases. It is concluded that the BSS has a high potential nutritional value as indicated by its medium protein content, reasonably high rumen degradability, and that inclusion of BSS in ammoniated rice straw diet is not only safe to animals, but also may improve growth rate of ruminants and feed conversion rate. It may be disadvantageous to use high amounts of BSS in ammoniated straw diets.

• Asian-Australasian Journal of Animal Sciences
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• 제15권1호
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• pp.26-33
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• 2002

In order to assess the effect of feeding raw or processed cotton (Gossypium) seed meal (CSM) on meat and wool production, 30 male crossbred lambs (3-4 months) of uniform body weight were assigned equally to five dietary treatments in a completely randomised design. The CSM was processed by three different methods i.e., cooking the meal at $100^{\circ}C$ for 45 minutes, treatment with 1% calcium hydroxide ($Ca(OH)_2$) for 24 h and iron treatment in the ratio of 1 part free gossypol (FG) to 0.3 parts of iron for 30 minutes. The lambs were fed isonitrogenous and isocaloric concentrate mixtures, containing 30% deoiled peanut meal (reference diet) and 40% of either raw, cooked, $Ca(OH)_2$ or iron treated CSM for 180 days. The raw and variously processed CSM replaced about 50% nitrogen of reference concentrate mixture. The concentrate mixtures were fed to meet 80% of the protein requirements (NRC, 1985) along with ad libitum chopped maize (Zea mays) hay. The slaughter weight, empty body weight and carcass weight was higher ($p{\leq}0.01$) in lambs fed cooked CSM incorporated diets, compared to diets containing deoiled peanut meal (DPNM). These parameters were not influenced by feeding diets containing either raw, $Ca(OH)_2$ or iron treated CSM in comparison DPNM diets. The carcass length, loin eye area and edible and inedible portion of carcass and the meat: bone ratio in whole carcass were also not affected by feeding CSM based diets. Among various primal cuts, the yield of legs was lower ($p{\leq}0.05$) from raw CSM fed lambs in comparison to DPNM fed lambs. The fat content in the Longissimus dorsi muscle was reduced ($p{\leq}0.05$) in lambs fed processed CSM based diets compared to those fed DPNM diet. Replacing DPNM with either raw or processed CSM based diets did not influence the sensory attributes and overall acceptability of meat. The wool yield was higher ($p{\leq}0.05$) in iron treated CSM fed lambs. The fibre length and fibre diameter were comparable among lambs on various dietary regimes. Among lambs fed variously processed CSM diets, the feed cost per kg of edible meat production was lower ($p{\leq}0.05$) on $Ca(OH)_2$ treated CSM, followed by cooked CSM diet and then on raw CSM based diets compared to DPNM diet. The CSM after 1% $Ca(OH)_2$ treatment or cooking for 45 minutes appears to be a satisfactory protein supplement in lamb diets for meat and wool production to replace at least 50% nitrogen of scarce and costly peanut meal.

• Asian-Australasian Journal of Animal Sciences
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• 제16권8호
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• pp.1143-1150
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• 2003

This study was conducted to investigate in vitro methane production of feed ingredients and relationship between the content of crude nutrients and methane production. Feed ingredients (total 26) were grouped as grains (5 ingredients), brans and hulls (8), oil seed meals (9) roughages (3), and animal by-product (1) from their nutrient composition and their methane production protential were measured by in vitro gas test. Among the groups, the in vitro methane productions for both 6 and 24 h incubation were highest in grains, followed by brans and hulls, oil meals and roughages, animal byproducts. Within the group of grains, methane production from wheat flour was the highest, followed by wheat, corn, tapioca, and then oat. Within the brans and hulls, soybean hull showed the highest methane production and cotton seed hull, the lowest. Methane production from oil meals was lower compared with grains and brans and hulls, and in decreasing order production from canola meal was followed by soybean meal, coconut meal, and corn germ meal (p<0.01). Three ingredients were selected and the interactions among feed ingredients were evaluated for methane production. Correlation coefficient between measured and estimated values of the combinations were 0.91. Methane production from each feed ingredient was decreased with increasing amount of crude fiber (CF), protein (CP) and ether extract (EE), whereas positive relationship was noted with the concentrations of N-free extract (NFE). The multiple regression equation (n=134) for methane production and nutrient concentrations was as follows. Methane production (ml/0.2 g DM)=(0.032${\times}$CP)-(0.057${\times}$EE)-(0.012${\times}$CF)+(0.124${\times}$NFE) (p<0.01; $R^2$=0.929). Positive relationship was noted for CP and NFE and negative relationship for CF and EE. It seems possible to predict methane production potential from nutritional composition of the ingredients for their effective application on formulating less methane emitting rations.

• Asian-Australasian Journal of Animal Sciences
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• 제14권1호
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• pp.21-29
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• 2001

An experiment was conducted with twenty crossbred male lambs to assess the effect of cotton (Gossypium) seed meal (CSM) on blood constituents and immunity. Lambs were randomly assigned to a reference diet (30% deoiled peanut meal, DPNM) and four test diets containing 40% of either raw, 45 minutes cooked, 1% $Ca(OH)_2$ and iron (1 free gossy-pol, FG : 0.3 Fe) treated CSM (replacing approximately 50%, reference concentrate mixture). These isonitrogenous and isocaloric concentrate mixtures were fed to meet 80% of protein requirements (NRC, 1985) along with ad lib maize hay for 180 days. Blood was collected at 60, 120 and 180 days post feeding. The lambs were sensitized with Brucella abortus S99 antigen after 140 days and were subjected to ELISA and delayed type hypersensitivity. Blood haemoglobin, erythrocyte count, leucocyte count, total protein, total albumin, total globulin, urea, creatinine concentration and aspartate aminotransferase activity in lambs fed on raw or processed CSM were comparable to the values of reference lambs. The higher (p<0.01) blood glucose levels observed in CSM fed lambs at 60 days of feeding was latter reduced to the levels comparable with those on reference diet at 120 and 180 days of feeding. The alanine amino transferase activity was lower in lambs fed raw and cooked CSM containing diets at 120 and 180 days of feeding. A marginal increase in serum iron and alkaline posphatase activity was observed in iron treated group and raw CSM fed lambs, respectively. The humoral immune response and DTH reactivity was lower (p<0.05) in lambs fed raw CSM (consuming 302.83 mg FG/day). Cooking, $Ca(OH)_2$ and iron treatment of raw CSM showed a positive response in alleviating the suppression of immune response owing to the reduced consumption of FG by 40.19, 17.40% and 26.73%, respectively in these diets. The present study thus indicated that consumption of 40% raw CSM (302.83 mg FG/day) though did not affect majority of the haematological and blood biochemical parameters, but markedly suppressed the immune mechanism of lambs.

• 한국양식학회지
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• 제11권1호
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• pp.19-29
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• 1998

참전복 사료에 적합한 단백질원을 조사하기 위하여 카제인, 어분, 육분, 우모분, 혈분, 대두박, 콘글루텐 밀, 면실박, 미역분을 첨가한 배합사료와 천연 먹이인 생미역과 건조 다시마를 선정하여 그 이용성을 비교하였다. 평균체중 0.11 g인 치패를 실험수조마다 70마리씩 수용하여 18주간 사육한 결과, 생존률은 카제인, 북양어분, 육분, 우모분, 미역분과 소맥분을 첨가한 실험구들이 서로 유의차없이(P>0.05) 타 실험구보다 높은 경향을 보였다. 실험구들의 최종평균체중은 0.27~0.74 g으로 북양어분을 첨가한 실험구가 0.74 g으로 가장 높았으며, 대두박 및 미역분말을 첨가한 실험구들이 북양어분 첨가구와 유의적인 차이없이(P>0.05) 양호한 결과를 보였다. 카제인 첨가구는 0.64 g으로 북양어분 첨가구와는 유의차를 보였으나(P<0.05). 다음으로 면실박과 소백분 첨가구가 0.55~0.57 g으로 양호한 성적을 보였으며, 육분 첨가구는 0.44 g, 천연 먹이인 생미역과 건조 다시마 공급구는 0.29~0.34 g, 우모분과 콘글루텐 밀 첨가구는 0.26~0.27 g으로 나타났으며, 혈분 첨가구가 0.19 g으로 가장 낮은 값을 보였다(P<0.05). 증체율과 각장 및 각폭도 평균체중 변화와 유사한 경향이었다. 실험 종료시 가식부의 수분과 단백질 함량은 각각 78.5~79.6% 및 13.2~14.2%로 사료의 단백질원에 거의 영향을 받지 않았다(P>0.05). 이상의 결과로부터 어분, 대두박, 면실박, 미역분말 또는 소백분은 참전복 사료의 훌륭한 단백질원이 될 것으로 기대되어 참전복 사료의 단가를 절감시키고, 사료의 질을 향상시키는데 큰 도움이 될 것으로 전망된다.