• Molecules and Cells
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• v.25 no.1
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• pp.43-49
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• 2008

Cancer Osaka thyroid, also known as Tpl-2 (Cot) is a member of the MAP3K kinase family and plays a key role in the regulation of the immune response to pro-inflammatory stimuli such as lipopolysaccharide (LPS) and tumour necrosis $factor-{\alpha}$ ($TNF-{\alpha}$). A series of Cot constructs with an N-terminal 6xHis tag were transiently expressed in HEK293 cells: $Cot_{130-399}$ (kinase domain), $Cot_{1-388}$ (N-terminal and kinase do-mains), $Cot_{1-413}$, $Cot_{1-438}$ (containing a putative PEST sequence), $Cot_{1-457}$ (containing both PEST and degron sequences) and $Cot_{1-467}$ (full-length protein). These Cot proteins were pulled down using an anti-6xHis antibody and separated by 2D electrophoresis. The gels were silver-stained and 21 proteins were detected that did not appear, or had substantially reduced intensity, in the control sample. Three of these were identified by MS and MS/MS analysis as Hsp90, Hsp70 and Grp78. Hsp90 appeared to bind to the kinase domain of Cot and this interaction was further investigated using co-immuno-precipitation with both overexpressed Cot in HEK293 cells and endogenous Cot in Hela cells.

• Journal of Microbiology and Biotechnology
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• v.26 no.7
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• pp.1267-1277
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• 2016

Currently, enzymatic synthesis of lactulose, a synthetic prebiotic disaccharide, is commonly performed with glycosyl hydrolases. In this work, a new type of lactulose-producing biocatalyst was developed by displaying β-galactosidase from Bacillus stearothermophilus IAM11001 (Bs-β-Gal) on the surface of Bacillus subtilis 168 spores. Localization of β-Gal on the spore surface as a fusion to CotX was verified by western blot analysis, immunofluorescence microscopy, and flow cytometry. The optimum pH and temperature for the resulting spore-displayed β-Gal was 6.0 and 75℃, respectively. Under optimal conditions, it showed maximum activity of 0.42 U/mg spores (dry weight). Moreover, the spore-displayed CotX-β-Gal was employed as a whole cell biocatalyst to produce lactulose, yielding 8.8 g/l from 200 g/l lactose and 100 g/l fructose. Reusability tests showed that the spore-displayed CotX-β-Gal retained around 30.3% of its initial activity after eight successive conversion cycles. These results suggest that the CotX-mediated spore-displayed β-Gal may provide a promising strategy for lactulose production.

• Journal of IKEEE
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• v.18 no.4
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• pp.593-602
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• 2014

One of the purposes of an artificial neural netowrk(ANNet) is to implement the largest number of functions as possible with the smallest number of nodes and layers. This paper presents a CoreNet which has a multi-leveled input value and a multi-leveled output value with a 2-layered ANNet, which is the basic structure of an ANNet. I have suggested an equation for calculating the capacity of the CoreNet, which has a p-leveled input and a q-leveled output, as $a_{p,q}={\frac{1}{2}}p(p-1)q^2-{\frac{1}{2}}(p-2)(3p-1)q+(p-1)(p-2)$. I've applied this CoreNet into the simulation model 1(5)-1(6), which has 5 levels of an input and 6 levels of an output with no hidden layers. The simulation result of this model gives, the maximum 219 convergences for the number of implementable functions using the cot(${\sqrt{x}}$) input leveling method. I have also shown that, the 27 functions are implementable by the calculation of weight values(w, ${\theta}$) with the multi-threshold lines in the weight space, which are diverged in the simulation results. Therefore the 246 functions are implementable in the 1(5)-1(6) model, and this coincides with the value from the above eqution $a_{5,6}(=246)$. I also show the implementable function numbering method in the weight space.

• Journal of Ginseng Research
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• v.8 no.2
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• pp.114-123
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• 1984

This study was conducted to investigated the morphology and distribution of starch granule in ginseng root. The results obtained are as follows; The starch contents of main and lateral ginseng 1$.$cot were 23-32% and 14-16%, respectively, and it was varied significantly with growing season, namely 15-37% in Summer (May to August) and 3-6% in Winter (November to March). Thus the starch content of ginseng root was different depending on the portien of ginseng root and growing seasons, but a significant difference was not observed by a growing period of ginseng. The starch granules showed nearly round or oval shape having the diameter 2-8${\mu}$ and their size were increased with a growing period of ginseng. The crystalline structure of starch granules was found to be B-type by X-ray diffraction study.

• Journal of IKEEE
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• v.15 no.4
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• pp.354-362
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• 2011

One of the unsolved problems in Artificial Neural Networks is related to the capacity of a neural network. This paper presents a CoreNet which has a multi-leveled input and a multi-leveled output as a 2-layered artificial neural network. I have suggested an equation for calculating the capacity of the CoreNet, which has a p-leveled input and a q-leveled output, as $a_{p,q}=\frac{1}{2}p(p-1)q^2-\frac{1}{2}(p-2)(3p-1)q+(p-1)(p-2)$. With an odd value of p and an even value of q, (p-1)(p-2)(q-2)/2 needs to be subtracted further from the above equation. The simulation model 1(3)-1(6) has 3 levels of an input and 6 levels of an output with no hidden layer. The simulation result of this model gives, out of 216 possible functions, 80 convergences for the number of implementable function using the cot(x) input leveling method. I have also shown that, from the simulation result, the two diverged functions become implementable by precalculating the weight values. The simulation result and the precalculation of the weight values give the same result as the above equation in the total number of implementable functions.

• Journal of the Mineralogical Society of Korea
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• v.16 no.1
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• pp.49-63
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• 2003

Rare and unusual occurrence of hydrothermal minerals were found in Geode mine area. They are developed in the late stage of hydrothermal alteration of earlier skarns and later by the open-space filling crystallization. The alteration of earlier skarns of clinopyroxene, garnet, and plagioclase formed mainly chlorite or sometimes uncommon hydrothermal minerals of prehnite, illite, and pumpellyite. Open-space filling crystallization characterized by hydrothermal minerals developedin open sapce or good are prehnite, pumpellyite, clinozoisite, illite, and Ca-zeolites of stilbite annstellerite. Mineral phases and paragenesis are examined in detail by microscopy, XRD, SEM, and EPMA. Using the Schreinemaker's method, equibrium reactions among these minerals are establishedand isothemal-isobaric phase diagrams of $\mu$$H_2O$-$\mu$$CO_2$cot are plotted. Hydrothermal minerals such asprehnite, pumpellyite, clinozoisite, illite, and some chlorite were ffrmed under high partial pressure of $CO_2$with relatively low $H_2$O fugacity. Later, stilbite and calcite in association with illite crystallized under relatively both high partial Pressure of $CO_2$and high $H_2$O fugacity.