• Molecules and Cells
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• v.20 no.3
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• pp.305-314
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• 2005

Investigation of chemically synthesized inositol 1,4,5-trisphosphate [$Ins(1,4,5)P_3$] analogs has led to the isolation of a novel binding protein with a molecular size of 130 kDa, characterized as a molecule with similar domain organization to phospholipase C-${\delta}1$ (PLC-${\delta}1$) but lacking the enzymatic activity. An isoform of the molecule was subsequently identified, and these molecules have been named PRIP (PLC-related, but catalytically inactive protein), with the two isoforms named PRIP-1 and -2. Regarding its ability to bind $Ins(1,4,5)P_3$ via the pleckstrin homology domain, the involvement of PRIP-1 in $Ins(1,4,5)P_3$-mediated $Ca^{2+}$ signaling was examined using COS-1 cells overexpressing PRIP-1 and cultured neurons prepared from PRIP-1 knock-out mice. Yeast two hybrid screening of a brain cDNA library using a unique N-terminus as bait identified GABARAP ($GABA_A$ receptor associated protein) and PP1 (protein phosphatase 1), which led us to examine the possible involvement of PRIP in $GABA_A$ receptor signaling. For this purpose PRIP knock-out mice were analyzed for $GABA_A$ receptor function in relation to the action of benzodiazepines from the electrophysiological and behavioral aspects. During the course of these experiments we found that PRIP also binds to the b-subunit of $GABA_A$ receptors and PP2A (protein phosphtase 2A). Here, we summarize how PRIP is involved in $Ins(1,4,5)P_3$-mediated $Ca^{2+}$ signaling and $GABA_A$ receptor signaling based on the characteristics of binding molecules.

• IMMUNE NETWORK
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• v.11 no.2
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• pp.114-122
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• 2011

Background: The leukocyte common antigen (CD45) is a transmembrane-type protein tyrosine phosphatase that has five isoforms. Methods: We generated seven murine mAbs against human CD45 by injecting cells from different origins, such as human thymocytes, PBMCs, and leukemic cell lines. By using various immunological methods including flow cytometry, immunohistochemistry, and immunoprecipitation, we evaluated the reactivity of those mAbs to CD45 of thymus as well as tonsil lysates. Furthermore, we transiently transfected COS-7 cells with each of gene constructs that express five human CD45 isoforms respectively, and examined the specificities of the mAbs against the transfected isoforms. Results: In case of thymocytes, lymphocytes, and monocytes, all the seven mAbs demonstrated positive reactivities whereas none was reactive to erythrocytes and platelets. The majority of immune cells in formalin-fixed paraffin-embedded thymus and tonsil tissues displayed strong membranous immunoreactivity, and the main antigen was detected near 220 kDa in all cases. Among the mAbs, four mAbs (AP4, DN11, SHL-1, and P6) recognized a region commonly present in all the five isoforms. One mAb, YG27, recognized four isoforms (ABC, AB, BC, and O). Two mAbs, P1 and P14, recognized the isoforms that contain exon A encoded regions (ABC and AB). Conclusion: In this study, we confirmed that AP4, DN11, SHL-1, YG27 and P6, are mAbs reactive with the CD45 antigen whereas P1 and P14 are reactive with the CD45RA antigen.

• Publications of The Korean Astronomical Society
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• v.14 no.2
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• pp.123-128
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• 1999

The beam size and the antenna efficiency of TRAO 14m radio telescope were measured at 86.2, 98.0 and 115.2 GHz. The beam size was determined by deconvolving the azimuthal scanning data of the Venus. To test the accuracy of our deconvolution method, the beam size at 86.2 GHz was compared with that determined from the mapping data of SiO maser line and we found no difference between them. With above beam sizes, antenna efficiencies were calculated from the continuum measurements of Venus, Jupiter and Moon. The beam efficiencies at these frequencies are appeared to be 0.49, 0.45 and 0.41, respectively. On the other hand, we also suggest that an automatic focus setting can be adopted for TRAO radio telescope based on the gradual change of best focus with elevation.

• Journal of Navigation and Port Research
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• v.27 no.1
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• pp.9-18
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• 2003

This is a study for international competitiveness analysis if korea ship management service using the collected data from the fields in industry. making a comparison if ship management company between domestic ones in Korea and mutinational ones in international market is more suitable for it's purpose. but competitiveness level of korean ship management companies are too low to compare. Therefore in this study, ship management devision in Korea overseas shipping companies are selected as a target of camparision and the representative of Korea ship management industry. The analysis of competitiveness is divided by price competition and non-price competition. The former is consist of element likes as crew management cost, dry-docking cost, repair cos, stores & spares cost, lubricants cost and management free cost, The later os consist of element like as quality of sea & shore personnel, efficiency of company's organization, ability of management system. qualifications & certifications, know -hoe for the ship management, structure of control & support ship and office automation & IT system. According to this analysis the ship management division in korean shipping companied are superior to the foreign specialized ship management companies generally. So, it is necessary that korea ship management industry attempt level-up continously and expand into international ship management market positively.

• KSBB Journal
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• v.13 no.2
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• pp.147-154
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• 1998

Enzymatic hydrolysis using an immobilized enzyme was carried out to produce chitosan oligosaccharides (COSs) from chitosan effectively. Chitosanase was immobilized on eight different carriers by physical adsorption. The enzyme immobilized on chitin had higher activity than those immobilized on the other carriers in spite of its lower adsorption. The activity of chitin-immobilized enzyme was more than 90% of the original activity. Optimal temperature of the immobilized enzyme increased by about $15^{\circ}C$ and its thermostability was excellent in relatively wide range of temperature. But its effects of pH did not improve compared to the free enzyme. The immobilized enzyme produced 153 mg/g chitosan of the reducing sugar for 3hrs of hydrolytic incubation time. The total content of higher oligomers, tetramer to hexamer, among amount of total COSs obtained for 2hrs was more than 90%. In kinetic parameters for both enzymes, immobilized enzyme showed lower affinity for substrate and reaction rate than free enzyme, however, no reduction of the rate for high substrate concentrations. Consequently, chitin-immobilized could effectively hydrolyse chitosan and produce the higher COSs without activity decrease in comparison with the free enzyme.

• Genomics & Informatics
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• v.12 no.4
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• pp.240-246
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• 2014

Mutation in HNF1B, the hepatocyte nuclear factor-$1{\beta}$ (HNF-$1{\beta}$) gene, results in maturity-onset diabetes of the young (MODY) 5, which is characterized by gradual impairment of insulin secretion. However, the functional role of HNF-$1{\beta}$ in insulin secretion and glucose metabolism is not fully understood. We identified a family with early-onset diabetes that fulfilled the criteria of MODY. Sanger sequencing revealed that a heterozygous P159L (CCT to CTT in codon 159 in the DNA-binding domain) mutation in HNF1B was segregated according to the affected status. To investigate the functional consequences of this HNF1B mutation, we generated a P159L HNF1B construct. The wild-type and mutant HNF1B constructs were transfected into COS-7 cells in the presence of the promoter sequence of human glucose transporter type 2 (GLUT2). The luciferase reporter assay revealed that P159L HNF1B had decreased transcriptional activity compared to wild-type (p < 0.05). Electrophoretic mobility shift assay showed reduced DNA binding activity of P159L HNF1B. In the MIN6 pancreatic ${\beta}$-cell line, overexpression of the P159L mutant was significantly associated with decreased mRNA levels of GLUT2 compared to wild-type (p < 0.05). However, INS expression was not different between the wild-type and mutant HNF1B constructs. These findings suggests that the impaired insulin secretion in this family with the P159L HNF1B mutation may be related to altered GLUT2 expression in ${\beta}$-cells rather than decreased insulin gene expression. In conclusion, we have identified a Korean family with an HNF1B mutation and characterized its effect on the pathogenesis of diabetes.

• Korean journal of applied entomology
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• v.46 no.2
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• pp.183-191
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• 2007

The external ultrastructural morphology and distribution of antennal sensilla and hair-pencils of Palpita indica (Saunder) were examined by scanning electron microscopy. The antennal flagellum of male and female has six types of sensilla: long trichodea, short trichodesa, chaetica, coeloconicum around spines, camaniformia and styloconica. There are sexual differences in antennae of P. indica. The female antenna has a coeloconicum sensillum without spines nearby the long trichodea sensilla on ninth flagellomere. Otherwise, auricillica sensilla are distribute on the basal segment of male antenna. These structures are not showed in the female antenna. There are a lot of pores on the hair tufts of the male hair-pencils.

• The Korean Journal of Soil Zoology
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• v.5 no.1
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• pp.21-32
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• 2000

A faunistic study on the spiders (Arachnida: Araneae) from Mt. Jumbong Kangwon-do was carried out from 1996 to 1999. During the survey period, 144 species of 88 genera belonging to 27 families including 5 undetermined species were identified from 900 collecting individuals. The dominant families were Linyphiidae 21 species; 14.6% of total species), Araneidae (20 species, 13.9%), Theridiidae (16 species, 11.1%), and Salticidae 12 species, 8.3%). The composition of the species of web builders was richer than that of hunters. As for species commonness with adjacent countries, the numbers of common species between Mt. Jumbong and Japan, Mt. Jumbong and China, and Mt. Jumbong and Russia were 15 species (80.4%), 98 species (68.5%), and 48 species (33.6%), respectively. The number of common species among these four regions were 39 species (27.3%). So, the spider species composition in Mt. Jumbong seemed being much similar to those in neighbor countries. An outline of biogeographical distribution of spiders can be summarized as 1 Cosmopolitan species (0.7%), 16 Palearctic species (11.2%), 7 Holarctic species (4.9%), 25 Korean endemic species (17.4%) and no Oriental species. In the spider fauna at Mt. Jumbong, therefore, there are high level of occurrence of Korean endemic species with northern spiders as Palearctic and Holarctic species. The level and direction of man-made development may have negative effect on regional biota.

• Korean Journal of Social Welfare Studies
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• v.48 no.1
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• pp.91-111
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• 2017

Purposes of this study, summoning thoughts and activities of Toynbee Hall of the UK and Hull House of the US held in between the end of 19C and the early 20C, are two folded: first, it is to examine the momentum and aims for which 'social' work was progressively established at that time; second, it is to look for implications for today's social work practice through understanding their characteristic activities and the context in which 'social' work was devised as an alternative in the two nations. The study method mainly relies on literature review, but further goes on to analyze the spirit of the age when settlement house was constructed as a welfare space and activities, leadership demonstrated in there, and to draw meanings for today, in terms of three dimensions: aim and location, professionality and education, and social action. Some of useful findings are: first, the COS and settlement house need to be considered in a continuum of socially responsive remedies against poverty and social work practice was developed in the process of 'suggestion-performance-critique-alternative suggestion-emergence of social work', rather than contrasting the two as opposite roots of social work practice. Second, settlement house was a socially constructed welfare space that contained intersectional dynamics of class, gender, personal vs. social, private vs. public, surrounding poverty issue. Third, besides differences between the two settlements, both purported for public goods and well-being and tried to realize the 'social' in that society. Lastly, this study explored historical meanings of settlement house as the welfare space with critical questions and discussed implications for social work practice today.

• The Journal of Korean Medicine
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• v.45 no.1
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• pp.17-43
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• 2024

Objectives: Underpinned by the context of a Korean traditional medicine cohort study on healthy aging, this research primarily aims to guide the selection of Clinical Outcome Assessments (COAs) for elderly healthy aging patient registry research, offering insights into the selection process; and secondly, to streamline the resource-intensive process of obtaining permissions for validated COAs, benefiting future traditional Korean medicine clinical researchers. Methods : In this study, we identified outcomes through a review of previous studies, followed by a process involving expert consultations to select the final outcomes. Subsequently, for the selected outcomes that were Clinical Outcome Assessments (COAs) developed tools, we searched in commercial databases to confirm the availability of Korean versions and the necessity of obtaining permissions. Finally, we obtained permissions for their utilization and, when needed, acquired the original instrument questionnaire through payment. Results: Through a literature review of existing observational studies, a total of 57 outcomes were selected, with 19 of them identified as COA instruments. Upon verifying usage permissions for these 19 instruments, it was found that 17 required author-specific permissions, and among these, 2 needed a purchase as they were commercially available. Conclusion: This study provides a detailed overview of outcome selection and permission acquisition for elderly patient registry research. It underscores the importance of Clinical Outcome Assessment (COA) tools and the rigorous approval process, aiming to enhance research reliability. Continuous verification of COA information is essential, and future research should explore Core Outcome Set (COS) development through consensus-building approaches like Delphi studies.