• Journal of Korean Biological Nursing Science
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• v.3 no.1
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• pp.1-10
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• 2001

뇌손상이라고 하는 과도한 stress를 받았을 때 free cortisol의 분비되는 양과 urinary free cortisol의 circadian 리듬에 어떻게 영향을 주는지 확인하기 위하여 시도하였다. 연구대상은 대조군은 건강한 젊은 여성 6명과 실험군응 CT상 뇌에 손상을 받은 4명의 여성으로 30대 환자이었다. 담당의사와 중환자실 관리책임자의 동의하에서 시도되었으며, 실험기간은 2000년 7월 1일에서 7월 10일까지였다. 대조군과 실험군의 뇨를 채취하여 뇨중 free cortisol 농도의 circadian rhythm을 알아보기 위해 채뇨 후 분석하였다. 채뇨는 뇌손상을 받고 응급실을 통해 신경외과 중환자실에 입원한 지 5시간 이내에 해당된 환자로 24시간 유지되는 foley catherization 상태하에서 12:00부터 3일동안 72시간을 2시간 간격으로 채뇨하였고, 대조군은 오전 12시부터 24시간 동안 2시간 간격으로 채뇨하였다. 측정방법으로는 cortisol의 정량은 solid-phase radioimmuoassay 방법을 이용하였으며, 분석재료는 Coat-A-Count(R) Cortisol kit(DPC, U.S.A.)을 사용하여 DPC사의 측정방법을 따랐다. 연구대상자의 free cortisol의 총량은 대조군에서는 $42.8{\mu}g$이었고, 실험군은 1일에 $991.2{\mu}g$, 2일에 $809{\mu}g$, 3일에 $544.2{\mu}g$으로 대조군과 통계적으로 유의한 차이를 나타내(p<.05), 실험군에서 현저하게 증가된 양상을 보였고, 시간이 지나면서 점점 감소하는 경향을 나타냈다. 시간별로 t-검정으로 분석한 결과로는 모든 시간대에서 대조군과 실험군의 평균치는 통계적으로 유의한 차이를 나타냈다. Free cortisol의 circadian에서는 대조군에서는 정상인의 cortisol의 circadian의 경우와 같은 리듬을 보였으나, 손상을 받은 실험군의 경우 분비량은 현저하게 증가했음을 보여주었다. 최고치가 제1일에 18:00과 다음날 10:00에 나타나 최고치가 2회 나타났으며, 제2일에도 제1일과 마찬가지로 18:00에 나타났고, 제3일에는 24:00에 나타나 제1일보다 제2일에는 최고치가 한 번 나타난 리듬을 보여주었고, 분비량은 2일에 감소하였다. 제3일에는 최고치가 8시간 지연된 나타난 리듬의 변화를 보여주었다. 최저치는 제1일, 제2일, 제3일 모두 24:00에 나타난 리듬을 보여주었다. 이상의 결과에서 실험군인 뇌손상 환자군에서는 뇌손상이 과도한 stress로 작용하여 환자의 free cortisol 분비량과 circadian 리듬에도 영향을 주는 것으로 나타났다. 그러므로 뇌손상환자를 간호하는 간호사는 스트레스상태인 것을 인지하여 환자 개개인에 필요한 간호를 해야 할 것으로 사료된다.

• The Journal of the Korean dental association
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• v.54 no.11
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• pp.897-906
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• 2016

Objectives: Periodontitis is multifactorial disease mainly caused by microbial community. Recently, some research has been conducted to find other possible risk factors including stress hormones related to periodontitis. Psychological stress can affect the periodontal health by a variety of biological mechanisms. This study compared the stress hormone levels in healthy subjects and patients with periodontal disease using saliva in order to investigate the association between periodontitis and stress. Methods: The human saliva was collected from 38 periodontally healthy individuals and 34 patients with chronic periodontitis under Institutional Review Board. Their age was 20-60 years ($40.3{\pm}10.45$). From these samples, determination of salivary levels of cortisol and Dehydroepiandrosterone (DHEA) performed by enzyme immunoassay kit (Salimetrics Europe, Suffolk, UK). The independent t-test and Mann-Whitney test for trend was applied using IBM SPSS statistics version 12.0 Program to analyze statistically significant differences. Results: Salivary cortisol levels of periodontitis patients were higher than those levels of healthy subjects (P < 0.001), while salivary DHEA levels of periodontitis patients were not significantly different (P = 0.431). Salivary cortisol/DHEA ratio of periodontitis patients was higher than those levels of healthy subjects (P < 0.001). Conclusions: Our study demonstrates the high levels of cortisol concentrations and cortisol/DHEA ratio in saliva of periodontitis patients than those of healthy subjects. Since cortisol levels and cortisol/DHEA ratio can be significant factors related to the severity of periodontal disease, our study would be helpful for early diagnosis and treatment of periodontal disease.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.7
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• pp.3167-3171
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• 2016

Prostate cancer (PCa) is one of the most common diseases in men. It is important to assess prognostic factors and whether high cortisol levels and complex hormonal interactions could be responsible for PCa development. We evaluated the relationship between cortisol, leptin and estrogens in 141 men, 71 with PCa and the remaining 70 constituting a low risk group (LRG). They were recruited for this study from a total of 2906 middle-aged men (ages 45-70 years) who completed an evaluation for prostatic diseases at the Urology Division, Hospital de Clínicas "$Jos{\acute{e}}$ de San $Mart{\acute{i}}n$", University of Buenos Aires, in May 2009. In this cross sectional study, cortisol, PSA, total-testosterone, free-testosterone, bioavailable testosterone, LH and estradiol were measured in serum. We observed increased cortisol levels in PCa patients as compared to LRG cases (p=0.004,). Leptin and estradiol levels were also higher in PCa patients (p=0.048; p<0.0001, respectively). Logistic regression analysis indicated that serum cortisol (OR: 1.110 (95% CI 1.016-1.213), p=0.022), estradiol (OR: 1.044 (95% CI 1.008-1.081), p=0.016) and leptin (OR: 1.248 (95% CI 1.048-1.487), p=0.013) explained 27% of the variance of dependent variables, even after adjusting for age, smoking, BMI and waist circumference. We found increased cortisol levels in PCa patients as compared to LRG, as well as an altered circulating hormonal profile.

• Korean Journal of Child Studies
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• v.33 no.3
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• pp.83-97
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• 2012

The purpose of this study is to investigate the effects of sand play therapy on the parenting stress and saliva cortisol levels of parents undergoing child counseling programs. The study was conducted with 13 parents who were undergoing child counseling programs. The sessions were held every week for 45 minutes from July, 2011 to September, 2011. To evaluate the effects of sand play therapy, pre test and post test were conducted and the results were then analyzed. The therapy sessions consist of parents making sand boxes, and this was done without consideration of any particular theme. The instrument used was Abidin (1990)'s Parenting Stress Instrument (PSI). Saliva cortisol levels were obtained at the pre-post stage of the sand play therapy. The data were analyzed by means of frequency and the Wilcoxon Rank Sum Test was conducted by the SPSS. The major findings were as follows; There were significant differences in terms of the decreases in the areas of perception of all the parenting stresses examined, including daily life stress, child temperament stress, child relationship stress, and learning-expectation stress, as well as in saliva cortisol levels. Finally, this study suggests that parenting stress and saliva cortisol levels have a positive relationship, and the effects of sand play therapy were significant with decreases in parenting stress and saliva cortisol levels. There is a clear need for parents undergoing child counseling programs to engage in sand play therapy to decrease parenting stress and saliva cortisol levels.

• Journal of Environmental Science International
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• v.29 no.5
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• pp.575-578
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• 2020

The plasma cortisol of nurserypigs was examined using an outdoor efficacy testwith a digital content-based approach in animal welfare convergence types. Nine nurserypigs,without discriminating between female and male, were classified into 2 groups of 3 pigs each: control and group 1 (effect+nature), control and group 2(effect+nature+music). The control group was the same for group 1 and 2 to compare the effects using a t-test. There was no significant difference in plasma cortisol levels between the control group and group 1 until 4 h after stress induction. However, significant differences were subsequently found between the control group and group 1 from 8 h to 72 h (p<0.05). Further, plasma cortisol was not affected in group 2 at 0 h through 8 h and 72 h. At 12 h through 48 h, group 2 showed a reduction in plasma cortisol level compared to the control group(p<0.05). These results indicated that after stress induction, applying effect plus nature or effect plus nature plus music can effectively decrease plasma cortisol levels in nursery pigs within8 h through 72 h and may serve as a better model for digital content-based approach in animal welfare convergence types.

• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.2
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• pp.311-317
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• 2011

The goal of this study was to clarify effects of sleep restriction on a diurnal rhythm of salivary cortisol and DHEA levels in Korean adolescents.83 middle school students were recruited to participate in restricted sleep period group (less than 4h/day) or unrestricted sleep period group (from 6h/day to 7h/day). Both were 14 to 17 years old. They were instructed to keep the sleep-awakening schedule and sampling protocol. Saliva samples of cortisol and DHEA were collected at 8h, 12h, 16h and 20h. Salivary hormones were analysed with salivary cortisol(or DHEA) EIA kit according to a fixed assay protocol. Cortisol levels of restricted sleep period group and unrestricted sleep period group significantly decreased according to the sampling times. Cortisol levels of sleep restricted group was significantly higher than those of usual sleep group at all sampling times. At 8h, DHEA levels of both groups were significantly higher than those at 12h, 16h and 20h. However, DHEA levels of restricted sleep period group did not differ from those of unrestricted sleep period group at all sampling times.Cortisol and DHEA levels of both group showed the typical diurnal rhythm regardless of sleep status. Restricted sleep may increased cortisol release, not DHEA release, which indicated a changed HPA axis.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.5
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• pp.734-741
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• 2019

Objective: The objective of the study was to determine the relationship between plasma and salivary cortisol concentrations in beef cattle that were subjected to handling prior to sampling. Methods: Twenty-one Nguni cows of three age categories; 5 to 7 yr (n = 7), 8 to 10 yr (n = 6), and 11 to 13 yr (n = 8) were handled for five consecutive weeks. In the pen, a human avoidance test was performed and cattle responses to restraint in the chute and crush were observed. In addition, rectal temperature readings were taken and, faecal samples were collected and analysed for glucocorticoid metabolites. Through the handling and restraint process, excretory and vocalisation behaviour, as a sign of stress were observed and recorded. Thereafter, six cows were randomly selected and subjected to an adrenocorticotropic hormone (ACTH) challenge. Blood and saliva samples were extracted to determine cortisol concentrations. Results: Repeated handling affected (p<0.05) faecal glucocorticoid metabolites, rectal temperatures, avoidance distance, crush scores as well as urination and defaecation behaviour. Acclimation to handling was variable based on each respective parameter. Saliva cortisol concentrations increased and decreased significantly (p<0.001). A peak value of $136.78{\pm}15.869nmol/L$ was observed 30min after administration of ACTH, from a baseline value of $8.75{\pm}15.869nmol/L$. Plasma cortisol concentrations did not differ (p>0.05) across the time of sampling. A low and insignificant correlation (r = 0.0131, p>0.05) between plasma and saliva cortisol was therefore observed. Conclusion: We conclude that if beef cows are subjected to handling prior to sampling, a weak relationship exists between plasma and salivary cortisol levels.

• The Korean Nurse
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• v.27 no.1 s.144
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• pp.51-56
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• 1988

Abnormal Cortisol and Thyroxine responses in depression or stress have extensively studied by many investigator. Cortisol hypersecretion and disturbed dexamethasone suppression are the most prominent in endogenous depressed patients. During stress, the am

• Journal of Aquaculture
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• v.17 no.3
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• pp.161-166
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• 2004

The influence of TBTO (Tributyltin-Oxide) at a series of concentrations (0.52, 1.41 and 3.05 $\mu$g/L) for a period of 3 weeks on estradiol-17$\beta$, testosterone and cortisol levels in male Korean rockfish, Sebastes schlegeli was investigated. Compared to the stable concentration of estradiol-17$\beta$, plasma testosterone level was decreased significantly at the 3rd week after TBTO exposure in plasma of fish (＞1.41 $\mu$g/L). The most profound physiological variation in TBTO exposed fish was a dramatic increase in plasma cortisol level at 3rd week. Conclusively, TBTO exposure caused increase level of cortisol as well as alteration of testosterone in the Korean rockfish. These results suggest that TBTO at environmentally relevant (nanomolar) concentrations disrupt endocrine secretions.

• The Journal of Internal Korean Medicine
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• v.10 no.1
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• pp.125-136
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• 1989

This study was investigated to clarify the effect of Sin Bee Tang (神秘湯) on the renal function, arterial blood pressure and plasma cortisol. The results obtained were follows; 1. Urine volume and glomerular filtration rate were decreased significantly after Sin Bee Tang water extract, 0.1ml/kg, administration. 2. Glomerular filtration rate, renal plasma flow and urinary excretion of electrolytes were increased significantly after Sin Bee Tang water extract, 0.25ml/kg, administration. 3. Plasma cortisol concentration increased significantly after Sin Bee Tang water extract, 0.25ml/kg, administration. These results suggest that the therapeutic action of Sin Bee Tang for 'Su Chun (水喘)' has a relation with the increase of plasma cortisol and renal hemodynamic effect.