• The Korean Journal of Zoology
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• 제38권3호
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• pp.433-441
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• 1995

Mammary tissue-specificity of the caprine $\beta$-lactoglobulin promoter appears to be secured by repression in non-expressing cells. In order to identify the mechanism of the negative regulation, the upstream promoter sequence of the caprine $\beta$-lactoglobulin gene was analyzed in detail. The repression was mediated by the upstream flanking sequence from -47O to -205. The sequence could repress the promoter activity of $\beta$-lactoglobulin in either orientation. The effect of the putative negative regulation element of caprine $\beta$-lactoglobulin on heterlogous promoters, however, varied: the promoter activity of herpes simplex virus thimidine kinase was either repressed or activated by the sequence depending on its orientation, while the SV4O early promoter was activated rather than repressed. The regulatory sequence involving the putative negative regulatory element was strongly shifted with the nuclear extract from non-mammary HeLa and CV-1 cells, while only weak shift was observed with that of mammary HC11 cells. Such correlation between repression and factor binding suggests that the protected regions in foot-printing assay may be the negative regulatory elements of $\beta$-lactoalobulin that serve tissue-specific repression.

• Journal of the Computational Structural Engineering Institute of Korea
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• 제25권3호
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• pp.227-235
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• 2012

The void distribution in concrete materials strongly affects its material properties. Therefore, the identification of spatial distribution of void is important to understand and estimate material behavior. To examine and quantify the void distribution inside lightweight aggregates, CT(computed tomography) image is used. 3D lightweight aggregate images are generated by stacking of cross-sectional images from CT. Spatial distribution of void of aggregate along the direction is visualized on the sphere using probability distribution function. Stiffness of lightweight aggregate for the directions is also examined. It is confirmed that direction-based probability distribution and stiffness from CT images are effective in characterizing void distributions of aggregates.

• Journal of Life Science
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• 제20권3호
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• pp.453-456
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• 2010

Leukemia is the abnormal increase of hematopoietic progenitor cells in tissues, resulting in anemia, increased susceptibility to infection and impaired blood clotting. The adenosine deaminase (ADA) gene is an important druggable target for the treatment of leukemia patients. Genetic and molecular analyses were performed to determine the effects of ADA gene mutations in 20 leukemia patients in the Korean population. To analyze the relationship between genotype and phenotype, the ADA genomic DNAs - including 1,092 bp of 12 exons and partial intron sequences flanking each exon - were sequenced and compared. In this study, the known mutations in other diseases, more than 50 mutations already reported in patients with severe combined immunodeficiency disease (SCID) and autism, were not found, but two novel mutations in leukemia patients were discovered. They include one nonsense mutation (A>C at nt position 478, F101F) and one missense mutation (G>A at nt position 778, E260K). One missense mutation (G>A at nt position 22, D8Y) was also detected in 20 normal control patients (allelic frequency of 7.5%). Interestingly, subjects in the Korean population retained 2 bp insertion at the intron 6 (IVS6-52insGC), something that has never been shown in other populations. The genetic study to find out the correlation between the mutant alleles and leukemia patients revealed no association statistically (p>0.05). The mutation found in leukemia needs further study to determine its possibility as a molecular marker for the diagnosis of leukemia.

• Molecules and Cells
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• 제19권3호
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• pp.428-435
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• 2005

This study was carried out to assess the potential of SSR markers for variety identification by comparing SSR markers and morphological traits in tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties. Twenty-seven SSR markers were polymorphic in 66 pepper varieties, revealing a total of 89 alleles. Average polymorphism information content (PIC) value was 0.529, ranging from 0.03 to 0.877. Cluster analysis of the band patterns separated the varieties into three groups corresponding to varietal types. Morphological trait-based clustering showed some degree of similarity to dendrogram topologies based on the SSR index. However, no significance correlation was found between the SSR and morphological data. SSR markers could be used to complement a DUS test of a candidate variety and to select complimentary varieties by pre-screening existing varieties in the context of protecting new varieties of pepper.

• Food Science of Animal Resources
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• 제40권5호
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• pp.758-771
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• 2020

This study was to investigate the prevalence and characteristics of antimicrobial-resistant Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) from 4,264 retail meat samples including beef, pork, and chicken in Korea between 2013 and 2018. A broth microdilution antimicrobial susceptibility testing was performed for S. aureus. Molecular typing by multilocus sequence typing (MLST), spa typing, and pulsed-field gel electrophoresis (PFGE), was performed on mecA-positive S. aureus strain. S. aureus was isolated at a rate of 18.2% (777/4,264), of which MRSA comprised 0.7% (29 strains). MLST analysis showed that 11 out of the 29 MRSA isolates were predominantly sequence type (ST) 398 (37.9%). In addition, ST72, ST692, ST188, ST9, and ST630 were identified in the MRSA isolates. The spa typing results were classified into 11 types and showed a high correlation with MLST. The antimicrobial resistance assays revealed that MRSA showed 100% resistance to cefoxitin and penicillin. In addition, resistance to tetracycline (62.1%), clindamycin (55.2%), and erythromycin (55.2%) was relatively high; 27 of the 29 MRSA isolates exhibited multidrug resistance. PFGE analysis of the 18 strains excluding the 11 ST398 strains exhibited a maximum of 100% homology and a minimum of 64.0% homology. Among these, three pairs of isolates showed 100% homology in PFGE; these results were consistent with the MLST and spa typing results. Identification of MRSA at the final consumption stage has potential risks, suggesting that continuous monitoring of retail meat products is required.

• Journal of Gifted/Talented Education
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• 제19권3호
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• pp.565-583
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• 2009

We studied what kind of science-contents problems can also give information on one's creativity for use in the selection of scientifically gifted. 383 7th and 8th graders as subjects, we first analyzed the corelation between the results of creative thinking, scientific attitude, science inquiry, logical thinking and the scientific creativity problem solving skill test. The corelation analysis indicated that science inquiry test could represent other tests except TTCT. We then tested 532 6th graders with TTCT and science inquiry tests concomitantly with the contents- related problems made in such types as naming 20 scientists, writing about a scientist, imagination to overcome a problem, understanding of theory and application and understanding of science laws and application. The results indicated that writing and imagination were most efficient in assessing both the contents and the creativity.

• Journal of the Korean Society of Food Science and Nutrition
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• 제29권6호
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• pp.1025-1029
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• 2000

This study was conducted to investigate whether a DNA comet assay could be applied for identifying irradiated pork and beef. Pork and beef were irradiated with Co-60 gamma rays at 0.1, 0.3, 0.5, 0.7 and 1.0 kGy, and stored in a freezer Cells separated from the samples were embedded in agarose gel on a slide, dissolved in a lysis solution, and electrophoresed at 2 V/cm for 2.0 min by horizontal electrophoesis. The cells were then stained with a silver staining in order to visualize the DNA using a micro-scope. The DNA fragments of the irradiated cells stretched or migrated out of the cells and formed tails towards the anode, giving the appearance of comets, while unirradiated cells formed very short or no tails. The distance of DNA migration increased with irradiation dose. Since the statistical analysis showed a significant correlation between tail length and irradiation dose, a DNA comet assay could provide not only identification but also estimation of the irradiation dose for irradiated beef and pork.

• The Journal of Industrial Distribution & Business
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• 제9권11호
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• pp.25-38
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• 2018

Purpose - This study aimed to identify the sub-dimensions of fashion social media quality (information quality, social quality, service quality, system quality) and investigate how they affect purchase intention through fashion information use behavior (information acceptance, information diffusion). Research design, data, and methodology - Data collection was carried out twice for systematic verification of the research model. In the first data collection, the reliability and validity of research variables were verified through 238 respondents and questionnaires were revised and supplemented based on their responses. In March 2018, the final survey was conducted from 755 respondents the age of 20 to 49. Using SPSS 23.0, descriptive statistics, exploratory factor analysis, correlation analysis were performed. In order to test hypotheses, structural equational modeling technique was employed using AMOS 23.0. Results - First of all, fashion Social media quality consists of four factors including information quality, social quality, service quality and system quality. Second, fashion Social media information quality, social quality, and system quality were shown to have a positive(+) effect on information acceptance behavior, and social quality, service quality and system quality were shown to have a positive(+) effect on information diffusion behavior. It was also determined that the acceptance and diffusion behaviors of fashion information through fashion Social media had positive(+) influence on purchase intention. Conclusions - This study holds academic significance in its identification of the components of fashion Social media quality and for conducting an empirical analysis on the causal relationship between fashion information acceptance and diffusion behaviors, and purchase intention. The results of this study indicate that fashion involvement is the key factors in determining the quality of Social media, the acceptance of information through Social media, and, by extension, the purchase of fashion products. Practitioners in the fashion industry may use the findings of this study in order to build more effective Social media strategy.

• Journal of Welding and Joining
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• 제35권3호
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• pp.82-87
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• 2017

Recent trends in shipbuilding and offshore industries are a huge increase in the ship size and the exploration and production of oil and natural gas in the arctic offshore region. High performance steel plates are required by these industrial trends. Also in IMO(International Maritime Organization) has begun to regulate of fuel of ship to environmental protection, therefore it is little bit difficult to use bunker-C oil to working ship. As the problem of environmental change such as global warming is emerged, the operation of the ship is considered to be involved in the environmental change problem, and the regulation of environmental pollution is gradually strengthened. As these environmental regulations are strengthened demand for LNG fuel ships is rapidly increasing. Currently, cryogenic steels used in LNG tanks include aluminum alloy, SUS 304, and 9%-Ni steel. Those steels are has high cost to construction of large LNG carrier. The new materials were suggested several steel mills to decrease construction cost and easy construction. The new cryogenic steel should be evaluate safety to applied real structure include LNG ship. Therefore, in this study, fracture toughness of weld joints were investigated with cryogenic steel for application of LNG tank.

• Journal of the Korea Convergence Society
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• 제8권3호
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• pp.49-61
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• 2017

Recently, convenience and usability are increasing with the development and deployment of various mobile applications on the Android platform. However, important information stored in the smartphone is leaked to the outside without knowing the user since the malicious mobile application is continuously increasing. A variety of mobile vaccines have been developed for the Android platform to detect malicious apps. Recently discovered server-based polymorphic(SSP) malicious mobile apps include obfuscation techniques. Therefore, it is not easy to detect existing mobile vaccines because some other form of malicious app is newly created by using SSP mechanism. In this paper, we analyze the correlation between the similarity of the method in the DEX file constituting the core malicious code and the permission similarity measure through APK de-compiling process for the SSP malicious app. According to the analysis results of DEX method similarity and permission similarity, we could extract the characteristics of SSP malicious apps and found the difference that can be distinguished from the normal app.