• Title/Summary/Keyword: Corn breeding

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Agronomic Characteristics of Waxy Hybrid Corn on the paddy soil conditions (논 토양조건에 따른 찰옥수수 교잡종의 작물학적 특성)

  • Ji, Hee Chung;Kim, Choong-Soo;Hong, Bom-Young;Lee, Hee-Bong
    • Korean Journal of Agricultural Science
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    • v.33 no.2
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    • pp.123-127
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    • 2006
  • This study was carried out to compare growth and yield performance for soil conditions at the Maize Genetics and Breeding Lab. in Chungnam National University. The waxy hybrid corn in the drained soil of paddy field showed higher fresh yield per 10a and 100-kernel weight per plant than control. The average dry yield of drained and drainless soil were 804kg/10a and 498kg/10a compared with a control (751kg/10a), respectively. The waxy hybrid corn in the drained soil also had better good stand and stay green characteristics than drainless soil condition, and showed moderately resistance to water stress and weed. Vegetational feature of weeds was characterized as the prevalence of Acalypha auatralis, Echinochloa crus-galli, Persicaria hydrpopiper in drained paddy soil and Persicaria hydrpopiper, Acalypha auatralis, Echinochloa crus-galli in drainless paddy soil, and Echinochloa crus-galli, Acalypha auatralis in upland soil.

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Genetic Distance and Heterosis Degree Among the Developed Waxy Corn Lines (찰옥수수 육성계통간 유전적 거리와 잡종강세 정도)

  • Lee, Moon-Sub;Yang, Jae-Hyeon;Lim, Seung-Bin;Lee, Hee-Bong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.60 no.4
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    • pp.464-469
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    • 2015
  • This study was carried out to find relationships of genetic distance and heterosis expression degree in the developed CNU waxy corn lines. The used material were developed in Corn Breeding Laboratory, Coll. of Agri. & Life Sci., CNU. Total 10 primers used for SSR maker analysis. In genetic distance, the used lines divided into four groups; A group has two lines, B group has one line, C group has ten lines and D group has eleven lines, respectively. Among used lines, CNU427 and CNU588 were very closed as a 74, while CNU451 and CNU429 were remote as a 40 inbred coefficient, respectively. While heterosis degree were very variable not only hybrids but also cross parents. Average heterosis of most cross-parent was high in plant height, ear height, flowering day and ear length. Especially, CNU H09-23 hybrid was high as 79.4% than other hybrids. Here, we gained the fact has closely relationship between genetic distance and heterosis.

Feed Value and Fermentation Quality of Covered Barley Grain Silage with respect to Days after Heading in Honam Region of Korea (호남지역 수확시기에 따른 겉보리 곡실발효사료의 사료가치 및 발효품질)

  • Park, Jong-Ho;Oh, Young-Jin;Cheong, Young-Keun;Song, Tae-Hwa;Park, Tae-Il;Lee, Kwang-Won;Kim, Kyong-Ho;Kim, Yang- Kil;Park, Jong-Chul;Kim, Bo-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.62 no.1
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    • pp.16-23
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    • 2017
  • This study aimed to determine the optimal harvesting time for covered barley to make grain silage, in Honam region of Korea. We harvested six varieties of barley every third day from 24 to 42 days after heading (DAH). The moisture content decreased from 62.4% at 24 DAH to 24% at 42 DAH. The moisture content at 36 DAH was 44.3%; however, moisture content at 39 and 42 DAH was lower than 40%. Yield of covered barley significantly increased from 24 to 42 DAH (p < 0.05). Yield at 36 DAH (557 kg/10a) was not significantly different from that at 39 and 42 DAH (p < 0.05). With respect to the feed value of barley grain silage, the amount of crude fiber and crude ash was different by harvesting time (p < 0.05). However, the amount of crude protein, crude fat, and total digestible nutrients (TDN) from 24 and 42 DAH was not significantly different. The pH of grain silage from 24 to 42 DAH was between 3.8 and 4.2 and it was stable until 36 DAH (p < 0.05). However, the pH of grain silage at 39 and 42 DAH was 5.2 and 5.8, respectively, which was higher than the pH of silage with good fermentation quality. The lactic acid content of barley grain silage from 24 to 42 DAH decreased from 5.5% to 0.5% (p < 0.05). The amount of lactic acid at 36 DAH was higher than that at 39 and 42 DAH (p < 0.05). With respect to moisture content, yield, feed value, and fermentation, the optimal harvesting time for grain silage of covered barley was 36 DAH. This could increase the use efficiency of harvesting machine for barley and reduce the harvesting time gap between whole barley silage and grain silage in Korea. Moreover, using barley grain silage for animal feed could reduce the import of corn.

Some Aspects of High Lysine Maize Breeding using Opaque-2 Gene (Opaque-2 인자를 이용한 고라이신 옥수수의 육종)

  • Bong-Ho Chae
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.5 no.1
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    • pp.57-64
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    • 1969
  • Several field and sweet corn varietiea from several sources were crossed with a variety carrying the opaque-2 gene to determine the phenotypic interactions in the breeding of high lysine maize. Although opaque-2 lines showed lower protein content than the corresponding normal varieties, there was no correlation between the protein levels of the two types. opaque-2 maize contained more lysine, but no relationship was found between the protein content and the lysine content of either normal or opaque-2 types, suggesting that high lysine corn using the opaque-2 gene may be developed independently from the protein content. The F2 segregation ratios for normal and opaque-2, 100-kernel weights, percentage seed set, opaque-2 phenotype, disease susceptibility, and the relationship between protein and lysine content of normal and opaque-2 were investigated. The determinations and observations were made on the F2, F3, and BC1 Lysine content was determined by the ion exchangeresin combined with paper chromatography method. Most crosses segregated in a 1-opaque-2 : 3-normal ratio as expected. Opaque-2 segregates were lighter than the normal type and smaller in size. A mottled phenotype of opaque-2 maize observed in the Philippines yellow endersperm. In some varieties opaque-2 maize was very susceptible to the ear and kernel rot disease. No. 5(female) and opaque-2(male). Selectlon of a double mutant of waxy and opaque-2 by using the iodine technique and electric lamp was discussed. opaque-2 and floruy-2 were not allels. Different percentage of seed set were observed in the segregation of aewx crossed with opaque-2. An unusual gametophytie relationship was involved in a cross between Glutinous.

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Effects of coated cysteamine hydrochloride on muscle fiber characteristics and amino acid composition of finishing pigs

  • Bai, Miaomiao;Liu, Hongnan;Xu, Kang;Yu, Rong;Oso, Abimbola Oladele;Deng, Jinping;Yin, Yulong
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.9
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    • pp.1430-1438
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    • 2019
  • Objective: This experiment was designed to determine the effects of coated cysteamine hydrochloride (CC) on muscle fiber characteristics, amino acid composition and transporters gene expression in the longissimus dorsi muscle (LDM) of finishing pigs. Methods: Two hundred and sixteen Duroc/Landrace/Yorkshire cross-bred male finishing pigs were fed with a corn-soybean basal diet supplemented with 0, 70, and 140 mg/kg cysteamine. Each group contained eight replicates of nine pigs per replicate. After 29 days, one pig was randomly selected from each replicate and slaughtered. Blood and LDM samples were collected and analyzed. Results: The results showed that supplemental dietary CC increased (p<0.05) the muscle fiber density. And CC supplementation also up-regulated (p<0.05) the expression of myosin heavy chain 1 (MyHC1) and MyHC2x mRNA levels, and down-regulated (p<0.05) MyHC2b expression in the LDM. Additionally, supplemental dietary CC reduced (p<0.05) the concentration of total cholesterol in the plasma and enhanced (p<0.05) the concentrations of essential amino acid and total amino acid in the LDM. The relative expression levels of chloramphenicol acetyltransferase 2, $b^{0,+}$ amino acid transporter, and $y^+$-L-type amino acid transporter 1 were upregulated (p<0.05) in the LDM when pigs were fed with the dietary CC of 70 mg/kg. Conclusion: Cysteamine supplementation could increase fiber density and distribution of fiber types. It also improved the deposition of protein in the LDM by up-regulated the expression of amino acid transporters.

Assessment and Classification of Korean Local Corn Lines by the Application of Principal Component Analysis (I) (Principal Component Analysis Method에 의(依)한 한국재래종(韓國在來種) 옥수수의 해석(解析) 및 계통분류(系統分類)(I))

  • Lee, In Sup;Choe, Bong Ho
    • Korean Journal of Agricultural Science
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    • v.8 no.2
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    • pp.139-151
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    • 1981
  • To obtain breeding materials 57 Korean local corn lines collected were assessed and classified by the application of principal component analysis. The results obtained were as follows. 1. In the result of principal component analysis for 27 characters, 67.1% and 88.6% of total variation could be appreciated by the first four and fir st ten principal component respectively. 2. According to the value of characters and principal components, contribution of characters to principal components were very variable. 3. Biological meaning of the principal component and plant type corresponded to the each principal component were explained clear by the correlation coefficients between principal components and characters. 4. 57 lines were classified into 4 lineal groups by the taxonomic distances.

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Feeding Value of Urea Treated Corncobs Ensiled with or without Enzose (Corn Dextrose) for Lactating Crossbred Cows

  • Khan, M. Ajmal;Sarwa, M.;Nisa, Mahr-Un;Khan, M. Sajjad
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.8
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    • pp.1093-1097
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    • 2004
  • This study was aimed to establish the amount of enzose (corn dextrose) for optimum N fixation in urea treated corncobs (UTC) and their dietary effect on nutrient intake, digestibility and milk yield in crossbred cows. Corncobs were treated with 5% urea and ensiled in laboratory silos with 0, 2, 4 and 6% enzose for 15 days. Total nitrogen (N), neutral detergent fiber (NDF) and neutral detergent insoluble N contents were increased in UTC with the level of enzose. Five early lactating crossbred cows (Sahiwal$\times$Holstein Frisian) were used in a 5$\times$5 Latin Square Design to see the influence of UTC ensiled with 6% enzose on nutrient intake, digestibility, milk yield and its composition. Five iso-nitrogenous and iso-caloric diets were formulated. The UTC30 (control), UTC40, UTC50, UTC60 and UTC70 diets contained 30% UTC ensiled without enzose and 40, 50, 60, 70% UTC ensiled with 6% enzose, respectively. Dry matter, NDF and ADF intakes were increased with the increasing level of UTC ensiled with enzose in the diets of cows. Dry matter, NDF and ADF digestibilities were significantly higher with diets containing UTC ensiled with enzose. Milk yield was significantly higher in cows fed UTC70 compared to those fed on other diets. The milk crude protein percentage was significantly different across treatments. However, milk fat, total solids, solid not fat, true protein and non-protein nitrogen contents of milk remained similar across all diets. Ensilation of UTC with 6% enzose improved the nitrogen retention and thus enhanced the feeding value of UTC for lactating cows.

Evaluation for Concentration of Residual PCDD/Fs in Pig Feed (돼지사료 중의 다이옥신 잔류 평가)

  • Ahn, Yun-Gyong;Shin, Jeoung-Hwa
    • The Korean Journal of Community Living Science
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    • v.23 no.2
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    • pp.129-136
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    • 2012
  • Dioxins are chemical substances that are not properly decomposed degradation under natural photochemical, biological and chemical conditions in the environment, and bio-accumulate through the food chain, so pose a risk of causing adverse effects to human health and the environment. Ninety seven percent of human exposure to dioxins comes through meat, fish, dairy consumption and so on. Pork is the largest proportion meat aspect of daily intake in the Korean food. Pigs are three times more than Cattle in terms of breeding population and production amount in the world, and the amount is increasing every year. Therefore, it is necessary to monitor the exposure level of dioxins in pig's body. Also, it is required to evaluate the presence of these chemical substances in the pig's feed grain such as corn, soybeans, and tallow. The purpose of this study was to evaluate PCDD/Fs in pig's feed to search the origin of PCDD/Fs. Feed samples obtained were wheat from East Europe, corn from South America and America, soybean meal from Korea, America, South America and India and tallow from Korea. The preparation of samples was based on the EPA method 1613. Instrumental analysis was based on the use of high resolution gas chromatography coupled to high resolution mass spectrometry (HRGC/HRMS). The concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 1,2,3,7,8-pentachlorodibenzo-p-dioxin (PCDD), which are the most toxic potent dioxins, were not observed in any of the samples. However OCDD was detected in most of the samples. The TEQs in the feed samples were ranged from 0 pg TEQ/g to 0.09841 pg TEQ/g. Especially, the high level of TEQs was observed in the samples of wheat from East Europe and soybean meal from Korea.

Consumption of Oxidized Soybean Oil Increased Intestinal Oxidative Stress and Affected Intestinal Immune Variables in Yellow-feathered Broilers

  • Liang, Fangfang;Jiang, Shouqun;Mo, Yi;Zhou, Guilian;Yang, Lin
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.8
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    • pp.1194-1201
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    • 2015
  • This study investigated the effect of oxidized soybean oil in the diet of young chickens on growth performance and intestinal oxidative stress, and indices of intestinal immune function. Corn-soybean-based diets containing 2% mixtures of fresh and oxidized soybean oil provided 6 levels (0.15, 1.01, 3.14, 4.95, 7.05, and $8.97meqO_2/kg$) of peroxide value (POV) in the diets. Each dietary treatment, fed for 22 d, had 6 replicates, each containing 30 birds (n = 1,080). Increasing POV levels reduced average daily feed intake (ADFI) of the broilers during d 1 to 10, body weight and average daily gain at d 22 but did not affect overall ADFI. Concentrations of malondialdehyde (MDA) increased in plasma and jejunum as POV increased but total antioxidative capacity (T-AOC) declined in plasma and jejunum. Catalase (CAT) activity declined in plasma and jejunum as did plasma glutathione S-transferase (GST). Effects were apparent at POV exceeding $3.14meqO_2/kg$ for early ADFI and MDA in jejunum, and POV exceeding $1.01meqO_2/kg$ for CAT in plasma and jejunum, GST in plasma and T-AOC in jejunum. Relative jejunal abundance of nuclear factor kappa B ($NF-{\kappa}B$) P50 and $NF-{\kappa}B$ P65 increased as dietary POV increased. Increasing POV levels reduced the jejunal concentrations of secretory immunoglobulin A and cluster of differentiation (CD) 4 and CD8 molecules with differences from controls apparent at dietary POV of 3.14 to $4.95meqO_2/kg$. These findings indicated that growth performance, feed intake, and the local immune system of the small intestine were compromised by oxidative stress when young broilers were fed moderately oxidized soybean oil.

Development of AFLP and STS Markers Related to Stay Green Trait in Multi-Tillered Maize

  • Jang Cheol Seong;Lee Hee Bong;Seo Yong Weon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.49 no.4
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    • pp.358-362
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    • 2004
  • In order to develop molecular markers related to stay green phenotype, AFLP analysis was conducted using near-isogenic lines for either stay green or non stay green trait. Both lines have characteristics of multi-ear and tillers (MET). Two out of 64 primer combinations of selective amplification identified three reproducible polymorphic fragments in MET corn with stay green. Both of E+AGC/M+CAC and E+AAG/M+CAA primer combinations produced two and one specific polymorphic fragments linked to stay green trait, respectively. For the conversion of AFLPs to sequence tag sites (STSs), primers were designed form both end sequences of each two polymorphic fragments. One fragment, which was amplified with E+AAG/M+CAA primer combinations, possessed 298 bp long and showed a $91\%$ homology with maize retrotransposon Cinful-l. One out of two polymorphic fragments produced with E+AGC/M+CAC primer combination had 236 bp long and matched a $96\%$ homology with an intron region of 22kDa alpha zein gene cluster in Zea mays. One out of two PCR fragments amplified with MET2 primer set in the stay green MET was not produced in the non-stay green MET. The developed AFLP and STS marker could be used as an efficient tool for selection of the stay green trait in the MET inbred.