• Mycobiology
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• 제47권4호
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• pp.466-472
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• 2019

For the purpose of protecting the rights of Lentinula edodes breeders, we developed a new simple sequence repeat (SSR) marker set consisting only of genetically independent tetranucleotide or longer core motifs. Using available genome sequences for five L. edodes strains, we designed primers for 13 SSR markers that amplified polymorphic sequences in 20 L. edodes cultivars. We evaluated the independence of every possible marker pair based on genotype data. Consequently, eight genetically independent markers were selected. The polymorphic information content values of the markers ranged from 0.269 to 0.764, with an average of 0.409. The markers could distinguish among 20 L. edodes cultivars and produced highly repeatable and reproducible results. The markers developed in this study will enable the precise identification of L. edodes cultivars, and may be useful for protecting breeders' rights.

• The Plant Pathology Journal
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• 제24권4호
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• pp.384-391
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• 2008

Rice blast disease, caused by the pathogenic fungus Magnaporthe grisea, is a serious issue in rice (Oryza sativa L.) growing regions of the world. Transcript profiling in rice inoculated with the fungus has been investigated using the transcriptomics technology, serial analysis of gene expression (SAGE). Short sequence tags containing sufficient information which are ten base-pairs representing the unique transcripts were identified by SAGE technology. We identified a total of 910 tag sequences via the GenBank database, and the resulting genes were shown to be up-regulated in all functional categories under the fungal biotic stress. Compared to the compatible interaction, the stress and defense genes in the incompatible interaction appear to be more up-regulated. Particularly, thaumatin-like gene (TLP) was investigated in determining the gene and protein expression level utilizing Northern and Western blotting analyses, resulting in an increase in both the gene and the protein expression level which arose earlier in the incompatible interaction than in the compatible interaction.

• 마이크로전자및패키징학회지
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• 제13권2호
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• pp.1-7
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• 2006

본 논문에서는 솔더 레지스트(solder resist)의 두께와 코어의 물성에 따른 인쇄회로기판의 철의 크기와 형상에 대하여 연구하였다. 인쇄회로기판의 굽힘 변형은 적층되는 재료의 열팽창계수의 차이에 의해 발생한다. 따라서 굽힘 변형의 감소를 위해서는 열팽창계수의 차이가 작은 적층 재료를 사용하는 것이 필요하며, 구조 형상에서도 상면과 하면의 불균일성을 완화시킬 필요가 있다. 또한, 적층 재료에서 코어의 강성을 높여 점의 발생을 억제할 수 있다. 코어를 이루는 복합재료는 적층 순서와 섬유 각에 따른 물성 특성의 방향성에 따라 굽힘과 비틀림이 연성되는 현상을 보이며, 이와 같은 성질을 이용하면 휨을 제어할 수 있다. 본 연구에서는 2층으로 구성된 chip scale package (CSP) 기판의 휨에 대한 연구로, 실험 및 유한 요소해석 툴을 이용하여 개선 결과를 도출하였다.

• Steel and Composite Structures
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• 제22권1호
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• pp.63-77
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• 2016

Top-down construction is a construction technique in which pit excavation and structure construction are conducted simultaneously. Reducing construction time and minimizing noise and vibration which affect neighboring structures, the technique is widely employed in constructing downtown structures. While H-steel columns have been commonly used as core columns, concrete filled steel tube (CFT) columns are at the center of attention because the latter have less axial directionality and greater cross-sectional efficiency than the former. When compared with circular CFT columns, square CFT columns are more easily connected to the floor structure and the area of percussion rotary drilling (PRD) is smaller. For this reason, square CFT columns are used as core columns of concrete encased and filled square (CET) columns in underground floors. However, studies on the structural behavior and concrete stress transfer of CET columns have not been conducted. Since concrete is cast according to construction sequence, checking the stress of concrete inside the core columns and the stress of covering concrete is essential. This paper presents the results of structural tests and analyses conducted to evaluate the usability and safety of CET columns in top-down construction where CFT columns are used as core columns. Parameters in the tests are loading condition, concrete strength and covering depth. The compressive load capacity and failure behavior of specimens are evaluated. In addition, 2 cases of field application of CET columns in underground floors are analyzed.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2005년도 제48회 춘계 학술연구 발표회
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• pp.36-36
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• 2005

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2004년도 International Conference of Sericultural and Entomological Sciences
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• pp.67-67
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• 2004

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2002년도 추계 합동 특강 및 학술발표대회
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• pp.143-143
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• 2002

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2005년도 제48회 추계 학술연구 발표회
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• pp.36-36
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• 2005

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2004년도 제47회 춘계 학술연구 발표회
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• pp.59-59
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• 2004

• 한국육종학회지
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• 제41권4호
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• pp.369-376
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• 2009

Cowpea might have been introduced from China to Korea and cultivated for several hundred years but it has never been a staple food crop in Korea. In this study, genetic diversity of 492 Korean cowpea landrace accessions that have passport information was estimated using six SSR markers. The mean of Weir's gene diversity was 0.665 from all accessions investigated in the study. Cowpea gene diversity of six local provinces in Korea was ranged from 0.370 in accessions of Gangwon to 0.680 in Jeonra provinces. Low gene diversity of the cowpea genepool of Gangwon province was probably derived from relatively few introductions. Especially SSR markers VM36 and VM39 seem to be good markers to distinguish the Gangwon accessions from others by occurring at a specific locus with higher than 78% of allele frequency. Except for the Gangwon province with the low genetic diversity, gene diversity of cowpea accessions from other provinces was ranged from 0.600 to 0.680 indicating no big differences among provinces. Distribution pattern of the allele frequencies was similar among the other provinces. This may reveal that Korean farmers might exchange cowpea seeds easily with even their neighbors with geographical barriers. A core collection, 100 landraces, ca. 20% of base collection, was developed at the 70% of a similarity coefficient level using random sampling approaches after stratification of the entire landrace collection based on the phenetic dendrogram. The variability of SSR in the base and core collections of Korean cowpea landrace was compared by calculating Weir's gene diversity. The mean of Weir's gene diversity of the core was 0.707 while that of the base collection was 0.665. The higher diversity index in the core collection indicates that it maintains the initial variability and well represents the base collection. The core collection included one of determinate accession (IT 216155) and two of no branching type accessions (IT 103959 and IT 161024). The core collection could be used to guide more efficient management and utilization of the entire collection. This core collection should be revised periodically as additional accessions are collected and further characterization is conducted.