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오끼나와해곡 남쪽해역의 저탁류 퇴적물의 특성 (Characteristics of a Turbidite Sediment from the Southern Margin of the Okinawa Trough, Japan)

  • 현상민
    • 한국해양학회지
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    • 제30권2호
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    • pp.69-76
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    • 1995
  • 오끼나와 해곡의 남쪽 해저 2051 m에서 채취된 저탁류 퇴적물(RN88-PC5)에 대하여 조사하였다. 반원양성 점토질 퇴적물과 천해에서 운반된 사질퇴적물 사이에는 저저성 유공충의 조성, 입도조성 및 화학적 원소의 조성 등에서 현저한 퇴적학적 특성의 차이 를 보인다. 저탁류 퇴적물인 사질퇴적물에 있어서는 점토질 퇴적물에는 포함되어 있지 않은 전형적인 신호초 해역의 유공충을 다량으로 함유하고 있는 것으로 보아 천해에서 유입된 퇴적물임이 거희확실하다. 이들 사질퇴적물은 저탁류와 관련되어 운반 되었으 며, 사질퇴적물과 점토질퇴적물 사이에는 다른 퇴적학적인 기작이 작용되었음을 시사 한다. /SUP 14/C 연대측정 결과와 유공충의 안정 산소동위원소값의 변화는 과거 약 1 만년으로부터 현재에 이르기까지 포층해수의 점진적인 온난화 경향을 보인다. 또한 Termination 1b의 기록을 보일 뿐만 아니라 약 2천년전과 7천년전의 2회에 걸친 담수 유입의 증거도 보여진다.

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Artomyces microsporus의 배양적 특성과 균사 적정 배양 조건 설정 (Culture Characteristics and Optimal Conditions for Mycelial Growth of Artomyces microsporus)

  • 민경진;이은지;박혜성;이찬중
    • 한국균학회지
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    • 제49권1호
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    • pp.45-55
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    • 2021
  • 본 연구에서는 A. microsporus의 최적 배양 조건을 구명하고자 하였다. A. microsporus 균사체의 효율적 배양을 위한 조건은 최적 배양 온도 25℃, pH 5.0, 탄소원 soluble starch 1%, 유기질소원 malt extract 1%, 무기질소원 NH4H2PO4 0.1%, 아미노산 asparatic acid 0.1% 조건으로 선발되었고 유기산, 무기염류 첨가 시 균사생육이 저조해지는 영향을 주어 선발되지 않았다. 새로이 SMNA로 명명한 A. microsporus의 최적 배지는 기존의 PDA 배지 상에서 배양한 결과와 비교하였을 때, 균사의 생육이 균일하고 안정적이며 배양 기간을 15.1% 정도 단축하는 효과가 있었다. SMNA 배지는 추후 A. microsporus의 균사체 배양 방법에 널리 활용될 수 있을 것으로 판단된다.

Haraldiophyllum hawaiiense sp. nov. (Delesseriaceae, Rhodophyta): a new mesophotic genus record for the Hawaiian Islands

  • Paiano, Monica O.;Huisman, John M.;Cabrera, Feresa P.;Spalding, Heather L.;Kosaki, Randall K.;Sherwood, Alison R.
    • ALGAE
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    • 제35권4호
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    • pp.337-347
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    • 2020
  • Haraldiophyllum hawaiiense sp. nov. is described as a new mesophotic alga and a new genus record for the Hawaiian Islands. Six specimens were collected at a depth range of 81-93 m from Papahānaumokuākea Marine National Monument, and their morphology investigated, as well as molecular phylogenetic analyses of the plastidial ribulose-1,5-bisphosphate carboxylase-oxygenase large-subunit (rbcL) gene and a concatenated alignment of rbcL and nuclear large-subunit rRNA gene (LSU) sequences. Phylogenetic analyses supported H. hawaiiense sp. nov. as a distinct lineage within the genus Haraldiophyllum, and sister to a large clade containing the type species, H. bonnemaisonii, as well as H. crispatum and an undescribed European specimen. The six Hawaiian specimens were shown to be identical, but unique among other species of the genus as well as the recently segregated genus Neoharaldiophyllum, which comprises half of the species previously included in Haraldiophyllum. The vegetative morphology of H. hawaiiense sp. nov. resembles Neoharaldiophyllum udoense (formerly H. udoensis); however, no female or post-fertilization structures were found in the Hawaiian specimens to allow a more comprehensive comparison. The molecular phylogenies demonstrate that Haraldiophyllum is paraphyletic, suggesting either that the Myriogrammeae tribe includes undescribed genera, including Haraldiophyllum sensu stricto, or that Neoharaldiophyllum species should be transferred into the genus Haraldiophyllum. However, based on vegetative morphology and molecular analyses, and pending resolution of this taxonomic issue, the Hawaiian specimens are placed within the genus Haraldiophyllum. This new record for the Hawaiian Islands highlights the novel biodiversity from mesophotic depths, reaffirming the need for further investigation into the biodiversity of Mesophotic Coral Ecosystems.

메타포를 적용한 정보시각화의 단순화 (The Simplification of information visualization using metaphor)

  • 김성곤
    • 문화기술의 융합
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    • 제7권3호
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    • pp.303-310
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    • 2021
  • 거시적 데이터 변화를 단순한 형태로 유추 비교 및 분석하는 시각 정보 콘셉트 개발방법이 필요하다. 시각 정보 콘셉트의 개발에는 시각화 형태 선택, 수사학적 효과 선택 그리고 디지털 표현요소 선택 작업이 필요한데, 이 중에서, 사용자에게 시각 정보를 효과적으로 전달하기 위한 수사학적 효과 선택 방법의 예를 제시한다. 본 연구에서는 기간별 및 업종별 주가 분석을 위해서, 원관념과 비유되는 보조관념을 통하여 거시적 관점에서 데이터 비교 분석이 가능한 메타포 수사학을 선택했다. 갓털이 퍼져나가는 민들레를 메타포로 사용한 2차원 3단 형태 변화와 시간에 변화 따라서 형태와 색상이 변하는 코랄 작약 꽃송이를 메타포로 사용한 3차원의 3단 형태 변화 정보표현 방식을 제시한다. 이러한 수사학적 메타포를 사용하면, 거시적인 거래 변화와 업종별 주가 비교 분석이 가능하다.

Micronesia, Chuuk Lagoon에 서식하는 파랑돔(Pomacentrus coelestis)의 생식년주기 (Annual Reproductive Cycle of the Neon Damselfish Pomacentrus coelestis, in Chuuk Lagoon, Micronesia)

  • 김한준;한정훈;허성표;이균우;최영웅
    • 한국수산과학회지
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    • 제54권2호
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    • pp.180-187
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    • 2021
  • This study was conducted to investigate the reproductive characteristics of the Neon damselfish Pomacentrus coelestis in Chuuk lagoon (7°27' N, 151°53' E), Micronesia, Equatorial Pacific Ocean. The specimens used in this study were collected from August 2009 to September 2010 from around the coral reef area at a depth of 3-10 m in the northwest coast of Weno Island. Overall, 228 individuals were collected over 14 months from this area, of which 115 were females and 108 were males. Among 108 males, 30 were found to be hermaphrodites and the sex of 5 individuals could not be determined. The overall sex ratio was approximately 1.06:1 (female:male), and the ratio of sexually active individuals was 0.79:1. The gonadosomatic index (GSI) fluctuated and showed the highest value of 8.43±1.21 in December 2009 and the lowest value of 3.36±0.71 in September 2009 in sexually active females. Males showed the highest GSI value of 2.64±0.93 in June 2010 and the lowest value of 0.35±0.06 in July 2010. Based on the fluctuation of GSI, the trends in the appearance of monthly sexually active individuals, and biopsy of the gonads, P. coelestis was found to be a hermaphrodite species of protogynous type and is considered to be reproductively active throughout the year.

A Review on Multidecadal Coastal Changes at Funafuti, Tuvalu from 1897 to 2015

  • Ahmed, Harun-Al-Rashid;Chan-Su, Yang
    • 대한원격탐사학회지
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    • 제39권1호
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    • pp.23-45
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    • 2023
  • Tuvalu is a small reef islands country in the Pacific Ocean. Its coastal regions are very much dynamic due to the profound effects of tropical cyclones and sea level rise (SLR). However, research works on coastline dynamics of Tuvalu mainly cover its capital, Funafuti. Therefore, this review summarizes the extent of long-term coastal changes in different islets of Funafuti and on overall Tuvalu. In Funafuti, highly accreting areas are Te Afualiku, Fuafatu, Motugie, and Amatuku, and highly eroding areas are Fuagea and Tefala with the fully disappeared islet of Vasafua after 2005. However, in spite of different causes and supposition of scientists on disappearing these lands the accretion is more dominant than erosion which resulted in 7.3% net increase of land areas of Tuvalu over 117 years till 2015. Severe tropical cyclones mainly caused accretion of land areas by forming coral rubble rampart formation and further reworks and erosion to small sandy islands whereas frequent low-energy cyclones mainly caused erosion. Though, till now severe erosion of coastal areas are not evident by global SLR, islets of Funafuti experienced remarkable shoreline increase as formation of 30-40 m wide rubble rampart formation along 19 km in 1971 by tropical cyclone Bebe and net increase of area of 3.45 ha by tropical cyclone Pam in 2015. In spite of such overall accretion of coastal areas several scientists suspect drowning of its areas in future because of high SLR (~5.1±0.7 mm/year) at Funafuti which supposedly will not work as a breakwater anymore. Thus, protection measures should be taken to prevent coastline erosion as well as land reclamation activities should be done following the global examples.

해안재해 대응 그린 인프라스트럭쳐의 국제 연구동향 분석 (An Analysis of International Research Trends in Green Infrastructure for Coastal Disaster)

  • 송기환;송지훈;석영선;김호준;이정아
    • 한국환경복원기술학회지
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    • 제26권1호
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    • pp.17-33
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    • 2023
  • Disasters in coastal regions are a constant source of damage due to their uncertainty and complexity, leading to the proposal of green infrastructure as a nature-based solution that incorporates the concept of resilience to address the limitations of traditional grey infrastructure. This study analyzed trends in research related to coastal disasters and green infrastructure by conducting a co-occurrence keyword analysis of 2,183 articles collected from the Web of Science (WoS). The analysis resulted in the classification of the literature into four clusters. Cluster 1 is related to coastal disasters and tsunamis, as well as predictive simulation techniques, and includes keywords such as surge, wave, tide, and modeling. Cluster 2 focuses on the social system damage caused by coastal disasters and theoretical concepts, with keywords such as population, community, and green infrastructure elements like habitat, wetland, salt marsh, coral reef, and mangrove. Cluster 3 deals with coastal disaster-related sea level rise and international issues, and includes keywords such as sea level rise (or change), floodplain, and DEM. Finally, cluster 4 covers coastal erosion and vulnerability, and GIS, with the theme of 'coastal vulnerability and spatial technique'. Keywords related to green infrastructure in cluster 2 have been continuously appearing since 2016, but their focus has been on the function and effect of each element. Based on this analysis, implications for planning and management processes using green infrastructure in response to coastal disasters have been derived. This study can serve as a valuable resource for future research and policy in responding to and managing various disasters in coastal regions.

Dihydroaustrasulfone alcohol induces apoptosis in nasopharyngeal cancer cells by inducing reactive oxygen species-dependent inactivation of the PI3K/AKT pathway

  • Kok-Tong Tan;Yu-Hung Shih;Jiny Yin Gong;Xiang Zhang;Chiung-Yao Huang;Jui-Hsin Su;Jyh-Horng Sheu;Chi-Chen Lin
    • The Korean Journal of Physiology and Pharmacology
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    • 제27권4호
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    • pp.383-398
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    • 2023
  • Dihydroaustrasulfone alcohol (DA), the synthetic precursor of a natural compound (austrasulfone) isolated from the coral species Cladiella australis, has shown cytotoxic effects against cancer cells. However, it is unknown whether DA has antitumor effects on nasopharyngeal carcinoma (NPC). In this study, we determined the antitumor effects of DA and investigated its mechanism of action on human NPC cells. The MTT assay was used to determine the cytotoxic effect of DA. Subsequently, apoptosis and reactive oxygen species (ROS) analyses were performed by using flow cytometry. Apoptotic and PI3K/AKT pathway-related protein expression was determined using Western blotting. We found that DA significantly reduced the viability of NPC-39 cells and determined that apoptosis was involved in DA-induced cell death. The activity of caspase-9, caspase-8, caspase-3, and PARP induced by DA suggested caspase-mediated apoptosis in DA-treated NPC-39 cells. Apoptosis-associated proteins (DR4, DR5, FAS) in extrinsic pathways were also elevated by DA. The enhanced expression of proapoptotic Bax and decreased expression of antiapoptotic BCL-2 suggested that DA mediated mitochondrial apoptosis. DA reduced the expression of pPI3K and p-AKT in NPC-39 cells. DA also reduced apoptosis after introducing an active AKT cDNA, indicating that DA could block the PI3K/AKT pathway from being activated. DA increased intracellular ROS, but N-acetylcysteine (NAC), a ROS scavenger, reduced DA-induced cytotoxicity. NAC also reversed the chances in pPI3K/AKT expression and reduced DA-induced apoptosis. These findings suggest that ROS-mediates DA-induced apoptosis and PI3K/AKT signaling inactivation in human NPC cells.

Kalkitoxin attenuates calcification of vascular smooth muscle cells via RUNX-2 signaling pathways

  • Saroj K Shrestha;Se-Woong Kim;Yunjo Soh
    • Journal of Veterinary Science
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    • 제24권5호
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    • pp.69.1-69.11
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    • 2023
  • Background: Kalkitoxin (KT) is an active lipopeptide isolated from the cyanobacterium Lyngbya majuscula found in the bed of the coral reef. Although KT suppresses cell division and inflammation, KT's mechanism of action in vascular smooth muscle cells (VSMCs) is unidentified. Therefore, our main aim was to investigate the impact of KT on vascular calcification for the treatment of cardiovascular disease. Objectives: Using diverse calcification media, we studied the effect of KT on VSMC calcification and the underlying mechanism of this effect. Methods: VSMC was isolated from the 6 weeks ICR mice. Then VSMCs were treated with different concentrations of KT to check the cell viability. Alizarin red and von Kossa staining were carried out to examine the calcium deposition on VSMC. Thoracic aorta of 6 weeks mice were taken and treated with different concentrations of KT, and H and E staining was performed. Real-time polymerase chain reaction and western blot were performed to examine KT's effect on VSMC mineralization. Calcium deposition on VSMC was examined with a calcium deposition quantification kit. Results: Calcium deposition, Alizarin red, and von Kossa staining revealed that KT reduced inorganic phosphate-induced calcification phenotypes. KT also reduced Ca++-induced calcification by inhibiting genes that regulate osteoblast differentiation, such as runtrelated transcription factor 2 (RUNX-2), SMAD family member 4, osterix, collagen 1α, and osteopontin. Also, KT repressed Ca2+-induced bone morphogenetic protein 2, RUNX-2, collagen 1α, osteoprotegerin, and smooth muscle actin protein expression. Likewise, Alizarin red and von Kossa staining showed that KT markedly decreased the calcification of ex vivo ring formation in the mouse thoracic aorta. Conclusions: This experiment demonstrated that KT decreases vascular calcification and may be developed as a new therapeutic treatment for vascular calcification and arteriosclerosis.

전남 화순군 백아산 아천동굴(석회동굴) 동굴생성물을 이용한 생광물화작용 연구 (Carbonate Biomineralization Using Speleothems and Sediments from Baekasan Acheon Cave (Limestone Cave) in Hwasun-gun, Jeollanam-do, South Korea)

  • 김유미;서현희;조경남;정다예;신승원;허민;노열
    • 한국광물학회지
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    • 제31권2호
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    • pp.113-121
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    • 2018
  • 전라남도 화순군에 위치한 백아산 아천동굴은 전남지역에서 발견된 유일한 석회동굴이다. 이 연구에서는 백아산 아천동굴 내부에서 채취한 동굴생성물(동굴산호, 붕암)과 주변 점토 퇴적물의 광물학적 특성을 확인하고, 생성물 내에 존재하는 호기성 미생물을 농화배양하여 탄산염광물을 형성하는 생광물화작용에 대해 알아보고자 하였다. 연구를 위한 시료는 동굴 내 세 지점에서 점토, 동굴산호, 붕암을 채취하였다. XRD 분석결과, 동굴산호와 붕암은 주로 탄산염광물인 Mg가 풍부한 방해석(Mg-rich calcite)으로 이루어져 있었고, 점토는 석영, 백운모, 질석으로 구성되어 있었다. 탄산염광물 형성 미생물의 농화배양을 위하여 각각 소량의 동굴생성물을 D-1 배지에 넣고 상온의 호기 조건에서 미생물을 배양하였다. 그리고 미생물들의 탄산염광물 형성능을 확인하고자 요소가 포함된 D-1 배지에 칼슘이온(Ca-acetate, Ca-lactate)을 주입한 후 각 시료로부터 농화배양된 미생물 배양액을 1% (v/v)씩 주입하였다. 그 결과 모든 조건에서 흰색의 침전물이 형성되었으며, XRD 분석결과 침전물이 방해석과 바테라이트(vaterite)로 구성된 것을 확인하였다. SEM-EDS 분석 결과 Ca, C, O를 주성분으로 하는 능면체, 구형, 주상형의 탄산칼슘이 관찰되었다. 따라서 백아산 아천동굴 내 영구암대에 분포하는 임의의 동굴생성물로부터 확인된 미생물들은 동굴생성물 형성에 관여하고 탄소와 칼슘의 지화학적 순환에 기여했을 것으로 추정된다.