• Journal of Microbiology and Biotechnology
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• 제22권1호
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• pp.156-158
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• 2012

A synthetic coprisin analog peptide, 9-mer dimer CopA3 (CopA3) was designed based on a defensin-like peptide, Coprisin, isolated from the bacteria-immunized dung beetle Copris tripartitus. Here, CopA3 was investigated for its antimicrobial activity and cancer cell growth inhibition. CopA3 showed antimicrobial activities against various pathogenic bacteria and yeast fungus with MIC values in 2~32 ${\mu}M$ ranges, and inhibited the cell viabilities of pancreatic and hepatocellular cancer cells, except MIA-Paca2, Hep3B, and HepG2 cells, in a dose-dependent manner. The average $IC_{50}$ values of CopA3 against pancreatic and hepatocellular cancer cells were 61.7 ${\mu}M$ and 67.8 ${\mu}M$, respectively. The results indicate that CopA3 has potential in the treatments of pancreatic and hepatocellular cancers as well as microorganism infection disease.

• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.264-269
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• 2012

Recently, we reported that the synthetic Coprisin analog peptide 9-mer dimer CopA3 (consisted of all-L amino acid sequence) was designed based on a defensin-like peptide, Coprisin isolated from Copris tripartitus. The 9-mer dimer CopA3 (L-CopA3) had antibacterial activity and induced apoptosis in human leukemia cells via a caspase-independent pathway. In this study, all of amino acid sequences of L-CopA3 were modified to all D-form amino acids (DCopA3) to develop a more effective antimicrobial peptide. We investigated whether D-CopA3 had antimicrobial activities against pathogenic microorganisms and pro-apoptotic effects in human leukemia cells (U937, Jurkat, and AML-2). The synthetic peptide D-CopA3 had antimicrobial activities against various pathogenic bacteria and yeast fungus with MIC values in the 4~64 ${\mu}M$ range. Moreover, D-CopA3 caused cell growth inhibition, and increased the chromosomal DNA fragmentation and the expression of inflammatory cytokines, TNF-${\alpha}$ and IL1-${\beta}$, transcripts in human leukemia cells. The all-D amino acid peptide DCopA3 proved as effective as the L-CopA3 reported previously. These results provide the basis for developing D-CopA3 as a new antibiotic peptide.

• Journal of Animal Science and Technology
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• 제64권6호
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• pp.1132-1143
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• 2022

Insects are a valuable natural source that can produce a variety of bioactive compounds due to their increasing species diversity. CopA3 is an antimicrobial peptide derived from Copris tripartitus (i.e., the dung beetle). It is known to increase the proliferation of colonic epithelial and neuronal stem cells by regulating cell cycle. This research hypothesized that CopA3 can promote the proliferation of porcine muscle satellite cells (MSCs). The effects of CopA3 on porcine MSCs, which are important for muscle growth and regeneration, remain unclear. Here, we investigated the effects of CopA3 on porcine MSCs. According to viability results, we designed four groups: control (without CopA3) and three treatment groups (treated with 5,10, and 25 ㎍/mL of CopA3). At a CopA3 concentration of 5 ㎍/mL and 10 ㎍/mL, the proliferation of MSCs increased more than that observed in the control group. Furthermore, compared to that in the control, CopA3 treatment increased the S phase but decreased the G0/G1 phase ratio. Additionally, early and late apoptotic cells were found to be decreased in the 5 ㎍/mL group. The expressions of the myogenesis-related transcription factor PAX7 and MYOD proteins were significantly upregulated in the 5 ㎍/mL and 10 ㎍/mL groups, whereas the MYOG protein remained undetected in all group. This study suggested that CopA3 promotes muscle cell proliferation by regulating the cell cycle of MSCs and can regulate the activity of MSCs by increasing the expressions of PAX7 and MYOD.

• 한국환경생태학회지
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• 제28권6호
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• pp.664-696
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• 2014

본 연구는 대청도와 소청도의 곤충다양성에 관한 연구를 위하여 실시하였으며 2011년 6월부터 10월까지 6회에 걸쳐 주간과 야간조사를 병행하여 실시하였다. 대청도에서 조사된 곤충은 총 9목 75과 286종이며 소청도에서 조사된 곤충은 총 8목 42과 106종으로 대청도와 소청도에서는 9목 78과 336종의 곤충이 서식하는 것으로 조사되었다. 기존 참고문헌을 포함하여 대청도와 소청도에 서식하는 전체 곤충을 정리하면 총 10목 103과 744종으로 나비목이 29과 439종으로 59%의 비율을 차지하며 우점하였고, 딱정벌레목이 27과 124종으로 16%의 비율로 조사되었다. 이 중에서 환경부 지정 멸종위기야생동물은 2종으로 애기뿔소똥구리와 왕은점표범나비가 확인되었다. 그 외 IUCN 적색목록에 포함된 종이 2종(봄처녀나비, 큰주홍부전나비), 국외반출승인대상종 25종, 고유종 5종, 한국적색목록 6종, 특정종 77종, 기후민감종이 2종으로 확인되었다. 본 조사에 새롭게 조사되어 신규등록된 곤충은 대청도에서 58과 147종이며 소청도에서 24과 43종으로 확인되었다.

• 한국환경생태학회지
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• 제25권4호
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• pp.526-539
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• 2011

본 논문은 물영아리오름 습지에 서식하는 곤충류를 파악하여 습지 보전에 대한 기초적인 정보를 제공하고자 실시되었다. 조사결과 총 9목 73과 229종이 조사되었으며, 물영아리오름 습지는 6목 39과 136종, 수령산은 9목 52과 121종이 조사되었다. 조사된 229종의 곤충류 중 멸종위기종인 애기뿔소똥구리가 포함되었다. 또한 오염되지 않은 습한 산 주위에서 주로 서식하는 늦반딧불이가 3개체 조사되었으며 이는 청정지역임을 나타내는 대표적인 종으로 습지에서 보호되어야 할 가치가 있는 것으로 판단된다. 또한 물영아리오름 습지의 나방류 확인을 위해 야간 유인등 조사를 실시하였으며 조사 결과 총 76종이 조사되었다. 넓은띠담흑수염나방이 0.12의 우점도 지수로 전체 우점종으로 조사되었으며, 종다양도는 7월 조사에서 3.42로 가장 높게 나타났다.