• Plant Resources
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• 제1권1호
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• pp.53-59
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• 1998

Laboratory experiments for the removal efficiency of heavy metals in land application of sludge, the accumulation and translocation of heavy metals in x plants after transplanting, and the responses of Minari growth with different ratio of land application of sludge were conducted to determine the potential ability of bioremediation with Minari plants. The removal rate and translocation of copper. zinc. lead. and cadmium in soil and plants were compared after transplanting the Minari plants to soil treated with different ratio of sludge. The removal efficiency of heavy metals in soil incorporated with sludge was different with application ratio, but increased with growing periods of Minari plants. The removal efficiency of Cu, Zn, Pb, and Cd ranged from 67 to 74% from 51% to 63%, from 37% to 71%. and from 15% to 25% after 45 days of transplanting. respectively. The amount removed the copper value. 65.9 mg/kg, observed to be highest in soil incorporated 3% sludge after 45 days. The translocation of Cu. Zn. Pb. and Cd from shoots to roots ranged from 18 to 53%, from 17 to 32%, from 14 to 49%, and from 23 to 38% over growing periods. respectively. In plant responses it appeared to be inhibited the plant growth in the treatment compared with the control at early stage of growth. However, the fresh weights of Minari plant increased from 12.5 to 62.5% in the sludge application after 45 days relative to the control. Therefore the Minari might play a useful role in bioremediation of Cu, Zn, Pb, and Cd in the land application of sludge.

• KSBB Journal
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• 제17권1호
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• pp.38-43
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• 2002

중금속 결합 단백질인 metallothionein (MT)를 발현하는 CUP1 유전자를 다중으로 함유하는 재조합 Saccharomyces cerevisiae의 균체성장과 중금속 제거특성을 조사하였다. CUP1 유전자를 다중으로 함유하는 재조합 효모는 중금속을 포함한 배지에서의 균체성장과 중금속 흡착능이 중금속에 대하여 내성을 가지고 있는 야생효모나 숙주세포에 비하여 우수하였다. 서로 다른 플라스미드를 함유하는 중금속을 함유하는 배지에서의 균체성장과 중금속 흡착능의 차이는 중금속에 대한 내성과 중금속 흡착에 MT 단백질의 발현 수준이 중요한 영향을 미치는 것으로 추정되었다. 구리를 함유하지 않고 고농도의 아연과 망간을 함유하는 배지에서 재조합 효모는 높은 균체 농도와 중금속 제거능을 나타내었는데, 이는 MT 단백질을 발현하는 CUP1 promoter가 아연과 망간에 의해서도 발현이 유도된다는 것을 알 수 있었다. MT 단백질을 효율적으로 발현하여 재조합 효모에 의한 중금속 흡착을 최대화 할 수 있는 최적의 구리농도는 0.31 mM로 결정되었으며, 비이온계 계면활성제인 Triton X-100은 재조합 효모의 균체성장과 중금속 흡착을 증가시켰지만, 대사 저해제는 균체성장과 중금속 흡착을 모두 저해하였다.

• 한국어병학회지
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• 제25권2호
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• pp.103-109
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• 2012

The objective of this study was to investigate the effects of exposured $Cu^{2+}$ on growth and bioaccumulation of abalone Haliotis discus hannai. Abalone were exposed to various concentration of $Cu^{2+}$ (0, 5, 10, 20, $40{\mu}g/L$). Bioaccumulation of tissues, hepatopancreas, muscles and gills were measured. hepatopancreas and gills $Cu^{2+}$ concentration of abalone increases to extent during the 4 weeks accumulation time. But muscles showed no significant changes, with respect to control. These data indicate that abalone Haliotis discus hannai hepatopancreas and gills can be considered adequate target tissues for waterborne exposured of $Cu^{2+}$. Weight growth rate of abalone exposed to $Cu^{2+}$ was significantly decreased in 20 and $40{\mu}g/L$ groups compared to control. This study revealed that high $Cu^{2+}$ concentration (${\geq}20{\mu}g/{\ell}$) reduced growth of abalone. These data indicate that excessive waterborne $Cu^{2+}$ can affect the toxicity of xenbiotics to abalone through alterations in growth rate. Thus, environmental standard of $Cu^{2+}$ $20{\mu}g/L$, should be considered a potential source of variation in toxicological studies with abalone.

• 대한가정학회지
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• 제29권2호
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• pp.47-53
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• 1991

A. niger H-18 and A. fumigatus E-29 wrer selected for their strong abilities to produce cellulase. The λd numerical values of the cotton fabrics inoculated with A. niger H-18 and A. fumigatus E-29 were 580 nm for the both strains of molds. By the growth of molds, lightness, original color scale, and grey scale of the fabrics gradually decreased while chroma increased. The minimum inhibitory concentrations of mold-proofing agents, such as Leperon WL, 8-hydroxyquinoline copper acetate, trimethylol melamine and dimethyl ethylene urea was 50 ppm. Glycoxale was not effective at the above mentioned concentration. Since Leperon WL, trimethylol melamine and dimethyl ethylene urea effectively inhibited the growth of A. niger H-18 and A. fumigatus E-29, tensile strength and elongation of the fabrics were not changed. however, cotton fabrics treated with glycoxale of the fabrics were not changed. However, cotton fabrics treated with glycoxale and inoculated with A. niger H-18 and A. fumigatus E-29 showed decreased in tensile strength by 31.1% and 33.9%, respectively.

• 아시안잔디학회지
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• 제12권4호
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• pp.195-202
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• 1998

The development of a protocol for high efficiency of embryogenic callus separation, maintenance and plant regeneration from the seeds of zoysiagrass cv. Zenith was studied. Embryogenic callus ratio is absolutely determined by genotype, but by adding high concentration of copper into medium, changing light condition and maintaining callus on initial induction medium for 8∼10 weeks, embryogenic callus can be easily distinguished and its growth can be promoted. There were significant differences among selected callus lines (each from one seed) according to their growth rates and regeneration percentages. Callus pre-treatment with activated charcoal inhibited callus growth, increased the level of precocious germination during culture and promoted shoot cluster formation after transfer to regeneration medium. For long-term callus maintenance, N6AA medium was better than MS medium, because the former inhibited non-embryogenic callus formation and kept vigor of embryogenic callus. The best callus lines Z-(5) has been successfully used for transformation and somaclonal variation selection in our laboratory.

• 대한가정학회지
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• 제31권2호
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• pp.213-219
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• 1993

Aspergillus fumigatus(AF) and Penicillum citrinum(PC) were selected for their strong abilities to produce protease. The result are summarized as follows; 1. The λd numerical values of the silk fabrics with AF and PC were 560 and 580 nm for the both stains of molds. 2. By the growth of molds, lightness, original color scale, and grey scale of the fabrics gradually decreased while chroma increased. 3. The minimum inhibitory concentrations of mold proofing agents, such as Leperon WL, 8-hydroxyquinoline copper acetates, trimethylol melamine and dimethly ethylene urea was 50 ppm. 4. Glycoxale was not effective at the abovely concentration. Since Leperon WL, dimethyl ethylene urea effectively inhibited the growth of PC silk, tensile strengh and elongation of the fabrics were not changed.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제44회 동계 정기학술대회 초록집
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• pp.590-590
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• 2013

Direct growth of graphene using CVD method has been done on CVD grown boron nitride substrate. From the SEM image, we have shown that the size of grain of graphene could be clearly controlled by varying the amount of injected hydrocarbon. To convince the existence of graphene on boron nitride, XPS and Raman has been checked. Both B1s and N1s peaks in XPS spectra and the Raman peak around 1,370 $cm^{-1}$ demonstrated that boron nitride did remain after high temperature treatment during the graphene growth process. And along the graphene grain boundary, the Raman fingerprint of graphene was neatly appeared.

• 한국축산식품학회지
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• 제28권1호
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• pp.27-31
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• 2008

본 연구는 사료내 형태별 구리와 아연 첨가가 비육돈의 생산성, 영양소 소화율 도체 및 육질 특성에 미치는 영향을 알아보기 위해 실시하였다. 3원 교잡종(Landrace x Yorkshire x Duroc) 비육돈 64두를 공시하였으며, 시험개시 시의 체중은 55.60 kg이었다. 1) 무기태 구리 $(CuSO_4{\cdot}5H_2O$ 30 ppm), 2) 유기태 구리(Cu-methionine, 30 ppm), 3)무기태 아연(ZnO, 80 ppm) 및 4) 유기태 아연(Zn-methionine, 80 ppm)으로 4개 처리를 하여 처리당 4반복, 반복당 4두씩 완전임의 배치하였다. 0-5주 동안 일당 사료 섭취량은 무기태 아연 처리구가 유기태 구리 및 아연 처리구와 비교하여 유의적으로 높게 나타났다(p<0.05). 그러나 전체 시험기간 동안 일당 증체량, 사료 섭취량 및 사료 요구율은 처리구간에 유의적인 차이를 보이지 않았다(p>0.05). 시험 5주째의 건물 및 질소 소화율은 처리구간에 유의적인 차이를 보이지 않았으나(p>0.05), 시험 10주째의 건물 및 질소 소화율은 유기태 아연을 첨가한 처리구가 다른 처리구와 비교하여 유의적으로 낮게 나타났다(p<0.05). 육의 pH는 Cu-Met처리구가 아연을 첨가 급여한 처리구와 비교하여 유의적으로 높게 나타났으며, 관능검사에서 육색은 Cu-met과 $CuSO_4$ 처리구가 Zn-met과 ZnO처리구와 비교하여 유의적으로 높게 나타났고(p<0.05), 근내지방도는 Cu-met 처리구가 다른 처리구와 비교하여 유의적으로 낮게 나타났으며(p<0.05), 경도에 있어서는 Zn-met 처리구가 유기태 구리 및 무기태 아연 처리구와 비교하여 유의적으로 높게 나타났다(p<0.05). 결론적으로 환경오염을 야기 시키는 무기태 구리 및 아연의 첨가 대신 유기태 구리 및 아연의 첨가는 성장 능력, 등심 단면적 및 육색에 영향을 미치지 않았다.

• 한국결정성장학회지
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• 제28권1호
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• pp.21-27
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• 2018

본 연구에서는 $Cu-BaFe_{12}O_{19}$계 강자성 복합재료를 제조하기 위하여 실온 기계적 합금화법(MA)을 적용하였다. 실험에서는 성분원소의 무게비를 각각 $Cu:BaFe_{12}O_{19}=4:1$, 3 : 2, 2 : 3 및 1:4으로 실시하였다. 모든 조성의 혼합분말에서 80분 동안 MA 처리에 의하여 금속 Cu에 $BaFe_{12}O_{19}$ 가 분산된 $Cu-BaFe_{12}O_{19}$ 복합재료를 제조할 수 있었다. Cu 및 $BaFe_{12}O_{19}$의 MA 처리 중 강자성 복합재료의 생성과정은 X선 회절분석 및 자기특성의 측정으로부터 자세히 관찰할 수 있었다. MA 처리로 얻어진 $Cu-BaFe_{12}O_{19}$ 복합재료의 자화값은 $BaFe_{12}O_{19}$ 양의 증가와 함께 점점 증가하였으며, 보자력 값은 모든 조성에서 MA 처리에 따른 $BaFe_{12}O_{19}$ 분말입자의 미세화에 따라 점점 감소됨을 알 수 있었다. 한편 $Cu:BaFe_{12}O_{19}=4:1$ 및 3 : 2 조성의 혼합분말의 경우 80분 동안 MA 처리하여 얻어진 $Cu-BaFe_{12}O_{19}$ 강자성 복합재료의 보자력 값이 각각 1400 Oe 및 1450 Oe로 여전히 높은 값을 보이는 사실로부터 Cu rich 조성 혼합분말의 경우 MA 처리 과정 중 강자성 $BaFe_{12}O_{19}$ 분말의 입자미세화가 연성 Cu의 존재에 의하여 크게 억제되어 비교적 큰 보자력 값을 보이는 것으로 판단된다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.128-128
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• 2017

Copper (Cu) is very toxic to plant cells due to its inhibitory effects on many physiological and biochemical processes. In spite of its potential physiological and economic significance, molecular characterization after Cu stress has so far been grossly overlooked in sorghum. To explore the molecular alterations that occur in response to copper stress, the present study was executed in ten-day-old Cu-exposed leaves of sorghum seedlings. The growth of shoots was markedly reduced, and ionic alterations were prominently observed in the leaves when the seedlings were exposed to different concentrations (0, 100, and $150{\mu}M$) of $CuSO_4$. Using two-dimensional gels with silver staining, 643 differentially expressed protein spots (${\geq}1.5-fold$) were identified as either significantly increased or reduced in abundance. Of these spots, a total of 24 protein spots (${\geq}1.5-fold$) from Cu-exposed sorghum leaves were successfully analyzed by MALDI-TOF-TOF mass spectrometry. Of the 24 differentially expressed proteins from Cu-exposed sorghum leaves, a total of 13 proteins were up-regulated, and 11 proteins were down-regulated. The abundance of most identified protein species, which function in carbohydrate metabolism, stress defense, and protein translation, was significantly enhanced, while that of another protein species involved in energy metabolism, photosynthesis and growth and development were severely reduced. The resulting differences in protein expression patterns together with related morpho-physiological processes suggested that these results could help to elucidate plant adaptation to Cu stress and provide insights into the molecular mechanisms of Cu responses in $C_4$ plants. The over-expression of GAPDH plays a significant role in assisting Sorghum bicolor to attenuate the adverse effects of oxidative stress caused by Cu, and the proteins involved in resistance to stress helped the sorghum plants to tolerate high levels of Cu.